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NANOPARTICLES
Contents:
Introduction Advantages and Disadvantages Methods of Preparation Characterization Evaluation Applications
INTRODUCTION
Nanoparticles are sub-nanosized colloidal structures composed
of synthetic or semi-synthetic polymers. Its size ranges from 1-100nm.
2.Nanocapsule
ADVANTAGES :
Reduction in the frequency of the dosages taken by the patient More uniform effect of the drug Reduction of drug Side Effects Reduced fluctuation in circulating drug levels Avoids hepatic first pass metabolism
DISADVANTAGES :
High cost Productivity more difficult Reduced ability to adjust the dose Highly sophisticated technology Requires skills to manufacture Difficult to maintain stability of dosage form.
Methods of preparation
A : Amphiphilic Macromolecules Cross Linking
1)Heat crosslinking. 2)chemical crosslinking.
B : Polymerization Methods
1) Emulsion polymerization 2) Dispersion polymerization 3) Interfacial condensation polymerization 4) Interfacial complexation
monomer supply
Catalyst
Nucleated micelle
Stabilized polymeric
micelle
(micelle)
(nanospheres)
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Dispersion polymerization:
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Nancapsules 30-300nm
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stirring
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Evaluation of nanoparticles :
1. Particle size :
Photon correlation spectroscopy(PCS) : For smaller particle. Laser diffractrometry : For larger particle. Electron microscopy (EM) : Required coating of conductive material such as gold & limited to dry sample. Transmission electron microscopy (TEM) : Easier method &
Permits differntiation among nanocapsule & nanoparticle .
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2.Density : Helium or air using a gas pycnometer Density gradiant centrifugation 3. Molecular weight : Gel permeation chromatography using refractive index detector. 4. Structure & Crystallinity : X-ray diffraction Thermoanalytical method such as, 1) Differential scanning calorimetry 2) Differential thermal analysis 3) Thermogravimetry 5. Surface charge: Surface charge of particle can be determined by measuring particle velocity in electrical field.
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6. Invitro release : Diffusion cell Recently introduced modified Ultra-filtration tech. Media used : phosphate buffer 7. Nanoparticle yield :
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Dry Powder Aerosol: L ung cancer treatment can be achieved by using nanoparticles in dry powder aerosol form .
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Applications:
Application Cancer therapy Purpose Targeting, Reduced toxicity, enhance uptake of antitumor agent Target reticuloendothelial system for intracellular infection Prolong systemic drug effect. Enhance immune response Enhanced bioavailability and significantly higher expression level Improved retention of the drug and reduced washed out. Material Polyalkylcyanoacrylate with anticancer agent Poly alkyl cyanoarylate
Poly methyl metha acrylate nanoparticles with vaccines DNA gelatin nanoparticles, DNA chitosan nanoparticles Poly alkyl cyanoacrylate nanoparticles , antiinflammatory agent
DNA delivery
Ocular delivery
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References:
Targetted and controlled drug delivery by S.P. VYAS and R.K. KHAR
Jain N.K. Advances in controlled and novel Drug Delivery, CBS publisher & Distributers, Edition 1st 2001, Pg. 408
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