ASSAY

LIST
Drug Amodiaquine and metabolite Amodiaquine, Chloroquine and metabolites Amoxicillin Artemether and dihydroartemisinin Artemisinin Matrix Plasma DBS Detection UV UV Sample volume 500 L 100 L 100 L 50 L 50 L 70 L 250 L 50 L 70 L 500 L 50 L 100 L 250 L 250 L 500 L 100 L 250 L 100 L 250 L Analogue Analogue Internal standard Range 4-4192 ng/mL 80-3450 nmole/L LLOQ 5 ng/mL 100 nmole/L

Plasma Plasma Plasma Blood

UV MS/MS MS/MS MS/MS MS/MS MS/MS MS/MS UV MS/MS MS/MS UV UV UV UV UV UV UV

Stable isotope labeled Artesunate Artesunate Stable isotope labeled Stable isotope labeled Stable isotope labeled Analogue Stable isotope labeled Stable isotope labeled Indomethacin Analogue Analogue Analogue Analogue Analogue Analogue

40-23000 ng/mL 1.12-575/ ng/mL 1.12-575 0.82-876.3 ng/mL 1.1-977 ng/mL 0.096-83.72/ 0.16-287.5 0.96-837.2/ 1.6-2875 1.1-977/ 1.56-2875 120-11500 ng/mL ng/mL ng/mL nmole/L

50 ng/mL 1.4/ 1.4 ng/mL 1.03 ng/mL 1.4 ng/mL 0.12/ 0.2 ng/mL 1.2/ 2 ng/mL 1.4/ 1.95 ng/mL 150 nmole/L 15 ng/mL 1.5 ng/mL 50 ng/mL 5 ng/mL 1 ng/mL 30 nmole/L 26 ng/mL 125 nmole/L 24/ 21 ng/mL

Artesunate and dihydroartemisinin

Plasma Plasma Blood

Atovaquone Azithromycin

Plasma Plasma Plasma

12-828 ng/mL 1.282.8 ng/mL 40-4600 ng/mL 4-23000 ng/mL 0.8-6900 ng/mL 24-3450 nmole/L 21-23000 ng/mL 100-23000 ng/mL 19.2-23000 ng/mL 16.8-1150 ng/mL

Carboxyprimaquine Chloroquine and metabolite

Plasma Whole blood Plasma DBS

Lumefantrine

Plasma DBS

Lumefantrine and Des-butylLumefantrine

Plasma

Drug Mefloquine Metabolite Oseltamivir and Oseltamivir carboxylate

Matrix Plasma, Whole blood & Serum Plasma Urine Saliva

Detection UV MS/MS MS/MS MS/MS UV MS/MS UV MS/MS UV MS/MS UV MS/MS MS/MS MS/MS

Sample volume 200 L 50 L 50 L 50 L 250 L 100 L 100 L 50 L 250 L 50 L 1000 L 50 L 50 L 50 L Analogue

Internal standard

Range 56-4600 ng/mL 0.8-345/ 8-11500 4-1725/ 24-34500 0.8-345/ 8-11500 12-4198 ng/mL ng/mL ng/mL ng/mL

LLOQ 70 ng/mL 1/ 10 ng/mL 5/ 30 ng/mL 1/ 10 ng/mL 15 ng/mL 3 ng/mL 25 ng/mL 1.5 ng/mL 10 ng/mL 1.5 ng/mL 60 ng/mL 48.8 ng/mL 0.95 ng/mL 0.05 g/mL

Stable isotope labeled Stable isotope labeled Stable isotope labeled Analogue Stable isotope labeled Analogue Stable isotope labeled Analogue Stable isotope labeled Analogue Stable isotope labeled Stable isotope labeled Stable isotope labeled

Piperaquine

Whole blood DBS DBS Plasma Plasma Urine Urine

2.4-1150 ng/mL 20-3450 ng/mL 1.0-661.3 ng/mL 8-5750 ng/mL 1.20-575 ng/mL 48-11500 ng/mL 39.04-57500 ng/mL 0.76-575 ng/mL 0.05-57.5 g/mL

Peramivir

Plasma Plasma

Zanamivir

Plasma

DBS = Dried blood spot