PRSS3 protease, serine, 3 [ Homo sapiens ]

Homo sapiens chromosome 9, GRCh37.p5 Primary Assembly

No mispriming library specified

Using 1-based sequence positions
OLIGO

start len

LEFT PRIMER
RIGHT PRIMER

tm

gc% any

3' seq

100 20 59.98 55.00 5.00 0.00 CGATCCAGTGCTTAGCTTCC

268 20 60.11 50.00 5.00 2.00 AGGTTTCCCTGGCTGAAACT

SEQUENCE SIZE: 1050

INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 169, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
1 TTTCCTTGTTAGCTGCGATACACAAATACATGAGCCAGATCCTTTCCTGAGGCCAGGAAG

61 CCTGGAATCTAATAACATTGGGCGGTGGATAAAGTCCCCCGATCCAGTGCTTAGCTTCCG
>>>>>>>>>>>>>>>>>>>>
121 TTAATGGAGCCATGGATGGAAGCGGATGTCAGGCGCAGTGGGGGAGAAATTTGCGGGGGT

181 GGCCCTGTCTAGGGCCAGAGAAACAACCTCTGAAGCTTAGATCCAGCCCTAGAGGGAAGA

241 AAGATGTGAGTTTCAGCCAGGGAAACCTAGCACTTTAAGAAATAGAAACCCTTTCAAAAT
<<<<<<<<<<<<<<<<<<<<
301 TTGAGGGCAGGTAGCTCTCCTAAAAGTTGCAGAAATGGCCACTTCTTTCACGTTCTCTTC

361 GACTGCTTTCTGCCGTGTCTGCCGTTTTCCTCCTCCCGCACCTTTTTGTCTCCTAGGGTG

421 GCATAGCCTTCCAAACAGATCTGCCTACAGCATTTTACCTGCAATTTTACACTGATTTTA

481 TAGAAAGGATGGCTTATTGTTCATGAGAAACGTGTACTGTTTTTAACCAGATGTGTTATA

541 TTTTCAGTATAACCTGGGTGTTGTGCAGTGGGAAATTACGGAAAGAATCAGGACACGTGG

601 TTAGGTAGGGGCCCAGTGGCGTCAGACTTAGCTCTTCGTGACTGCGAGCTTCTTGGTTTA

661 ATAAGGATATTGGCTCGCTCTGTTCCTGGGGTTTCTGGGAAAAGATATGGAACTGAAATG

721 AAGGTTTCATTGAATATGGCCCACTGATTTTACAAGCAGAGGTGCCTGAGATAAGCAAAA

781 GGTGAGGCATGACCTCTGGCTGTGCATTATTTGTTCTGATATTACAGGAGCCAAGCATTC

841 CACTTCCTTCTTTTTAAAGAGCTTTGATTTCCTCTCACATCTTCATGAAGAAGGAAACAG

901 TGCCCCCACTTACTTATCCTCAGGCCACACCTGTTCCCATTTATTCCGTGAGTTCCTCCC

961 GCACCCTTCTGGTTGATGTTGGTTGTGATGGCCGAGGCGAAGTTATTGAATCTCACATTT

1021 GGCACCAGTTGTTTCTGTTTCTCATCTCCC

KEYS (in order of precedence):

>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS

start len

tm

1 LEFT PRIMER

gc% any

3' seq

100 20 59.98 55.00 5.00 0.00 CGATCCAGTGCTTAGCTTCC

RIGHT PRIMER

342 20 60.12 45.00 6.00 2.00 GTGGCCATTTCTGCAACTTT

PRODUCT SIZE: 243, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER

99 20 59.98 55.00 5.00 1.00 CCGATCCAGTGCTTAGCTTC

RIGHT PRIMER

342 20 60.12 45.00 6.00 2.00 GTGGCCATTTCTGCAACTTT

PRODUCT SIZE: 244, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
3 LEFT PRIMER

72 20 60.04 45.00 3.00 2.00 ATAACATTGGGCGGTGGATA

RIGHT PRIMER

268 20 60.11 50.00 5.00 2.00 AGGTTTCCCTGGCTGAAACT

PRODUCT SIZE: 197, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER

565 20 59.94 45.00 4.00 3.00 GCAGTGGGAAATTACGGAAA

RIGHT PRIMER

795 20 60.11 50.00 8.00 0.00 AGGTCATGCCTCACCTTTTG

PRODUCT SIZE: 231, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
Statistics
con too

in

in

no

sid many tar excl bad

ered
Left

tm

tm high high

GC too too any

high
3' poly end

Ns get reg GC% clamp low high compl compl

X stab

7966

28

0 2587 3089

0 144 2116

Right 7875

28

0 2599 3064

0 151 2030

Pair Stats:
considered 307, unacceptable product size 292, high end compl 1, ok 14
primer3 release 1.1.4

(primer3_results.cgi release 0.4.0)

ok

Trp1 Translocation protein 1 [ Drosophila melanogaster ]

Drosophila melanogaster chromosome 2L, complete sequence
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
319 20 59.99 50.00 4.00 2.00 AAGTCTGGAGGCGACAAGAA
RIGHT PRIMER
606 20 59.98 55.00 2.00 2.00 GCCAAGTAGCAGGATGAAGC
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 288, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
1 TTCTCTTTTTTCCTCTTATAGGAATACACCGAACCGGGTGCCCAGAAGGTAGACAAGCCG
61 TCAAAGGACGAGAAGAACGTGGCCAAGTGGCTGAAGAAGAACGTTAAGACGAAGAAGACC
121 AAATTCCTGAGCCACATCGTCGAATACTTCACCTCCAGCAAGGCCATCGATGCTCTGCTA
181 AAGTCCAAATTCACCGAAGGCAGCAATCCGCTTTTCACCACCCGGGAGCAGGTAATCGAG
241 TTTCTAGACGTGATGCTGGAGCACAAGTTCTTCCATCGCGCCAAGAAGGTGCCCGTCACT
301 CTGGAGGAGATCAGAGGAAAGTCTGGAGGCGACAAGAAGGCGGACAAGGAGAAGACCCAA
>>>>>>>>>>>>>>>>>>>>
361 GATAAGGAAAAGGACAAGGCAAAGGATGAGAAGAAGGATACCGATCCGGAAGGCGATAAT
421 GGCCAAGGAGACGGAGGTGCGTCTGGCAACGAAAAAGAGAAGGAGAAAAAGGAGAAGAAA
481 AAGCGCAAGATCCGCCTGGACATGCATCCTGAGCAGATCTTCGTGGACGGCTCCGAGGCA
541 TACGTCTGGATCTACGATCCCATACCGCTCCACTACTGGATATTCGGCTTCATCCTGCTA
<<<<<<<<<<<<<<
601 CTTGGCGCCGTTGGCATTTGCCTATTCCCTCTGTGGCCTCCACTGCTACGCAAGGGTGTC
<<<<<<
661 TACTACTTGTCCATTGCGGCTGCCGGCTTCCTTGTTTTCATCCTGGCTCTGACAATTGTA
721 CGTCTAATTGTGTTCATCATCGTGTGGGCATTAACCGGCGGCAAGTTGCACTTCTGGATC

781 TTCCCCAACCTGACTGAGGATGTGAGCTTCTTCGCCTCCTTCTGGCCACTATATGAGGTT
841 TGTTTCGAATTATCCATTTATGTTAGTTTACTAAATTACCTTCATCTCTAGAGCAACTAT
901 AACAGTGACCCAAATAATTCGTCCGCCAAGACCGACAAGAAATCGAAATCGAAGGACAAG
961 AAGAAGGAAAAAGAAAGCGATGCCGAGGACACAGCCGTGGATGCGGATGGAGACGCTGAC
1021 GGTGATGTGGATGCTGAGGTTTCCACGCTT
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
317 20 59.99 55.00 3.00 2.00 GAAAGTCTGGAGGCGACAAG
RIGHT PRIMER
606 20 59.98 55.00 2.00 2.00 GCCAAGTAGCAGGATGAAGC
PRODUCT SIZE: 290, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER
584 20 59.98 50.00 2.00 0.00 TCGGCTTCATCCTGCTACTT
RIGHT PRIMER
788 20 60.04 50.00 4.00 0.00 TTGGGGAAGATCCAGAAGTG
PRODUCT SIZE: 205, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
3 LEFT PRIMER
583 20 59.98 50.00 2.00 1.00 TTCGGCTTCATCCTGCTACT
RIGHT PRIMER
788 20 60.04 50.00 4.00 0.00 TTGGGGAAGATCCAGAAGTG
PRODUCT SIZE: 206, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
4 LEFT PRIMER
317 20 59.99 55.00 3.00 2.00 GAAAGTCTGGAGGCGACAAG
RIGHT PRIMER
570 20 60.06 55.00 4.00 2.00 GAGCGGTATGGGATCGTAGA
PRODUCT SIZE: 254, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8252 0 0 0 50 0 1966 4371 0 1 13 151 1699
Right 8416 0 0 0 50 0 1933 4541 0 1 0 139 1752
Pair Stats:
considered 126, unacceptable product size 107, high any compl 2, ok 17
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

DMPK dystrophia myotonica-protein kinase [ Homo sapiens ]

Homo sapiens chromosome 19, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER 68 20 59.95 60.00 2.00 1.00 AGGCAGAGAGAAGGGGAGAC
RIGHT PRIMER 244 20 60.07 50.00 5.00 3.00 CGTCCGATCACCTTCAGAAT
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 177, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
1 CAGAACCTTACACGGTGTTTATGTGGGGAAGCCCCTGGGAAGCAGACAGTCCTAGGGTGA
61 AGCTGAGAGGCAGAGAGAAGGGGAGACAGACAGAGGGTGGGGCTTTCCCCCTTGTCTCCA
>>>>>>>>>>>>>>>>>>>>
121 GTGCCCTTTCTGGTGACCCTCGGTTCTTTTCCCCCACCACCCCCCCAGCGGAGCCCATCG
181 TGGTGAGGCTTAAGGAGGTCCGACTGCAGAGGGACGACTTCGAGATTCTGAAGGTGATCG
<<<<<<<<<<<<<<<<
241 GACGCGGGGCGTTCAGCGAGGTAAGCCGAACCGGGCGGGAGCCTGACTTGACTCGTGGTG
<<<<
301 GGCGGGGCATAGGGGTTGGGGCGGGGCCTTAGAAATTGATGAATGACCGAGCCTTAGAAC
361 CTAGGGCTGGGCTGGAGGCGGGGCTTGGGACCAATGGGCGTGGTGTGGCAGGTGGGGCGG
421 GGCCACGGCTGGGTGCAGAAGCGGGTGGAGTTGGGTCTGGGCGAGCCCTTTTGTTTTCCC
481 GCCGTCTCCACTCTGTCTCACTATCTCGACCTCAGGTAGCGGTAGTGAAGATGAAGCAGA
541 CGGGCCAGGTGTATGCCATGAAGATCATGAACAAGTGGGACATGCTGAAGAGGGGCGAGG
601 TGAGGGGCTGGGCGGACGTGGGGGGCTTTGAGGATCCGCGCCCCGTCTCCGGCTGCAGCT
661 CCTCCGGGTGCCCTGCAGGTGTCGTGCTTCCGTGAGGAGAGGGACGTGTTGGTGAATGGG

721 GACCGGCGGTGGATCACGCAGCTGCACTTCGCCTTCCAGGATGAGAACTACCTGGTGAGC
781 TCCGGGCCGGGGTGACTAGGAAGAGGGACAAGAGCCCGTGCTGTCACTGGACGAGGAGGT
841 GGGGAGAGGAAGCTCTAGGATTGGGGGTGCTGCCCGGAAACGTCTGTGGGAAAGTCTGTG
901 TGCGGTAAGAGGGTGTGTCAGGTGGATGAGGGGCCTTCCCTATCTGAGACGGGGATGGTG
961 TCCTTCACTGCCCGTTTCTGGGGTGATCTGGGGGACTCTTATAAAGATGTCTCTGTTGCG
1021 GGGGGTCTCTTACCTGGAATGGGATAGGTC
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
112 20 59.84 50.00 4.00 0.00 TTGTCTCCAGTGCCCTTTCT
RIGHT PRIMER
221 20 59.99 60.00 6.00 2.00 GAAGTCGTCCCTCTGCAGTC
PRODUCT SIZE: 110, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER
111 20 59.84 55.00 4.00 0.00 CTTGTCTCCAGTGCCCTTTC
RIGHT PRIMER
221 20 59.99 60.00 6.00 2.00 GAAGTCGTCCCTCTGCAGTC
PRODUCT SIZE: 111, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
3 LEFT PRIMER
202 20 59.99 60.00 6.00 3.00 GACTGCAGAGGGACGACTTC
RIGHT PRIMER
373 20 60.22 55.00 6.00 2.00 AGCCCAGCCCTAGGTTCTAA
PRODUCT SIZE: 172, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER
202 20 59.99 60.00 6.00 3.00 GACTGCAGAGGGACGACTTC
RIGHT PRIMER
372 20 60.22 60.00 6.00 3.00 GCCCAGCCCTAGGTTCTAAG
PRODUCT SIZE: 171, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 7829 0 0 0 275 0 689 5723 0 1 43 167 931
Right 7796 0 0 0 285 0 717 5639 0 4 61 158 932
Pair Stats:
considered 118, unacceptable product size 104, high end compl 4, ok 10
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

MBNL1 muscleblind-like splicing regulator 1 [ Homo sapiens ]

Homo sapiens chromosome 3, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
826 20 60.11 50.00 4.00 0.00 TCACACCTGTAATCCCAGCA
RIGHT PRIMER
926 20 59.97 55.00 3.00 1.00 GTAGAGACGGGGTTTCACCA
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 101, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
1 TTAACGCAGTATTCTATTCAGCAATGGTTCCATATTTTCCATATGTTTATAGATAACATT
61 CAGATATCGAATTTTCATATATGTTTTAATTCATTTATTTATTTCTTTTATCTAGGAGAA
121 ATATAATGGTTTCCAAATGTCCTATACTAGATTATTGATAGATTTTGTCATTAGTTTTAC
181 TATTGAATACCATGAAATATCAGCTGTTGCAGCCACTAAATAGTTACAAACTGTTGGTAC
241 TACAGTTTACTAGTCAATCCATGGTTATATGCTTGTGCTATGCTAAATATAAAGGATTCA
301 AGATGAGGACTTAAGCAGTTCATATTTGTATTTAGAATATTATCAAAGATATTTTCTTAA
361 CTTGTCTCAGTATTAGAATTTACAGCCCATTTTCAGCTTATTGTATCCAGAAATTTCAAT
421 CATATCCAATAATTTATCAGCATTTGTTGATGACAGTAAAAGTGACACATTAGCATTTTA
481 TTTGTTTCTGTGTTGTTTGATTACAACAGTGACATTTTAGCACTCTTTGTGGACGGATAA
541 CTCTTCTAGTAGAAAGAAAGCAGGAGGGTGGTCAAGCTAAGTGACAGATACAAAGAGTTG
601 GAGGACATAGGGCTTTCTGTGGGTAAGAAATGGATGAGAAAAGCTTTATGTCACTGACTA
661 GGCTTGCAATTCAGTATTCCCCTATCAAAGGCCCCCTTACTGATGTTTCTGTTTACCAGA
721 TCTTGGTTTCAAGATGCCAGGTTCTGAAGAGATACACCACGAGTTCTTATAAACACTATA

781 TCTAAATTTGGTTTGAAAGTCTAATGTTTGGCTGTGTGCAGTGGCTCACACCTGTAATCC
>>>>>>>>>>>>>>>
841 CAGCACTTTGGGAGGCCGAGGCAGGCAGATCACGAGGTCAGGAGATTGAGGCCGTCTTGG
>>>>>
901 CCAACATGGTGAAACCCCGTCTCTACTAAAATACAAAAAATTAGCTGGGCATGGTGGCGC
<<<<<<<<<<<<<<<<<<<<
961 ATGCCTGTAATCCCAGTTACTTGGGAGCTGAGGCAGGGGGATTGCTTGAACTCGGGAGGC
1021 GGAGGTTGCAGTGAGCTGAGATCATGCCAC
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
826 20 60.11 50.00 4.00 0.00 TCACACCTGTAATCCCAGCA
RIGHT PRIMER
956 20 59.96 45.00 4.00 2.00 CACCATGCCCAGCTAATTTT
PRODUCT SIZE: 131, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER
553 20 60.11 50.00 2.00 0.00 AAAGAAAGCAGGAGGGTGGT
RIGHT PRIMER
837 20 59.90 55.00 4.00 2.00 TTACAGGTGTGAGCCACTGC
PRODUCT SIZE: 285, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER
689 20 59.83 50.00 4.00 0.00 AGGCCCCCTTACTGATGTTT
RIGHT PRIMER
926 20 59.97 55.00 3.00 1.00 GTAGAGACGGGGTTTCACCA
PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER
688 20 59.83 50.00 5.00 0.00 AAGGCCCCCTTACTGATGTT
RIGHT PRIMER
926 20 59.97 55.00 3.00 1.00 GTAGAGACGGGGTTTCACCA
PRODUCT SIZE: 239, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8940 0 0 0 835 0 4799 1409 0 1 21 65 1810
Right 8852 0 0 0 652 0 4413 1848 0 1 20 79 1839
Pair Stats:
considered 34, unacceptable product size 27, ok 7
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) [ Homo sapiens ]
Homo sapiens chromosome 3, GRCh37.p5 Primary Assembly

Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
35 20 59.99 50.00 7.00 3.00 TGACTGGCATTCAAGCTGTC
RIGHT PRIMER
324 20 60.10 55.00 4.00 1.00 CCCTCCGTACCAGTTCTCAA
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 290, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
1 GAAGAGACCCAGCAACCCACAGAGTTGAGAAATTTGACTGGCATTCAAGCTGTCCAATCA
>>>>>>>>>>>>>>>>>>>>
61 ATAGCTGCCGCTGAAGGGTGGGGCTGGATGGCGTAAGCTACAGCTGAAGGAAGAACGTGA
121 GCACGAGGCACTGAGGTGATTGGCTGAAGGCACTTCCGTTGAGCATCTAGACGTTTCCTT
181 GGCTCTTCTGGCGCCAAAATGTCGTTCGTGGCAGGGGTTATTCGGCGGCTGGACGAGACA
241 GTGGTGAACCGCATCGCGGCGGGGGAAGTTATCCAGCGGCCAGCTAATGCTATCAAAGAG
301 ATGATTGAGAACTGGTACGGAGGGAGTCGAGCCGGGCTCACTTAAGGGCTACGACTTAAC
<<<<<<<<<<<<<<<<<<<<
361 GGGCCGCGTCACTCAATGGCGCGGACACGCCTCTTTGCCCGGGCAGAGGCATGTACAGCG
421 CATGCCCACAACGGCGGAGGCCGCCGGGTTCCCTGACGTGCCAGTCAGGCCTTCTCCTTT
481 TCCGCAGACCGTGTGTTTCTTTACCGCTCTCCCCCGAGACCTTTTAAGGGTTGTTTGGAG
541 TGTAAGTGGAGGAATATACGTAGTGTTGTCTTAATGGTACCGTTAACTAAGTAAGGAAGC
601 CACTTAATTTAAAATTATGTATGCAGAACATGCGAAGTTAAAAGATGTATAAAAGCTTAA
661 GATGGGGAGAAAAACCTTTTTTCAGAGGGTACTGTGTTACTGTTTTCTTGCTTTTCATTC

721 ATTCCAGAAATCATCTGTTCACATCCAAAGGCACAATTCATTTTGAGTTTCTTTCAAAAC
781 AAATCGTTTGTAGTTTTAGGACAGGCTGATGCACTTTGGGCTTGACTTCTGATTACCCTA
841 TTGTTAAATTAGTGACCCCTCTTAGTGTTTTCCTGTCCTTTATTTCGGAGGACGCACTTC
901 GAAGATACCAGATTTTATGGGTCATCCTTGGATTTTGAAGCTTATAACTGTGACAAAAAA
961 TGTGAAGGGAAGAGATTTGAAACATGTGGAAGGAAAAGTGAGTGCAGACTATAAACTTCC
1021 AAAAAGACAAGCCCAAAATACACCTAAACG
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
35 20 59.99 50.00 7.00 3.00 TGACTGGCATTCAAGCTGTC
RIGHT PRIMER
248 20 59.86 55.00 3.00 3.00 TTCACCACTGTCTCGTCCAG
PRODUCT SIZE: 214, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER
35 20 59.99 50.00 7.00 3.00 TGACTGGCATTCAAGCTGTC
RIGHT PRIMER
148 20 59.83 50.00 3.00 3.00 TTCAGCCAATCACCTCAGTG
PRODUCT SIZE: 114, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER
798 20 59.87 50.00 5.00 1.00 AGGACAGGCTGATGCACTTT
RIGHT PRIMER
1038 20 60.08 40.00 2.00 0.00 TTTTGGGCTTGTCTTTTTGG
PRODUCT SIZE: 241, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
4 LEFT PRIMER
229 20 59.86 55.00 3.00 0.00 CTGGACGAGACAGTGGTGAA
RIGHT PRIMER
514 20 60.07 55.00 2.00 0.00 GGGGAGAGCGGTAAAGAAAC
PRODUCT SIZE: 286, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8237 0 0 0 135 0 2610 3634 1 2 40 135 1679
Right 8318 0 0 0 135 0 2842 3363 0 0 44 123 1811
Pair Stats:
considered 99, unacceptable product size 85, ok 14
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

INSR insulin receptor [ Homo sapiens ]

Homo sapiens chromosome 19, GRCh37.p5 Primary Assembly

Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
438 20 59.98 55.00 4.00 2.00 CCTTTCTCCTTCTGCACCTG
RIGHT PRIMER
544 20 60.07 45.00 4.00 0.00 TGGCAAGCCCTGTTAAAATC
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 107, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
1 TTCACTTCTTCTTCCATATAAGCCATGTTACAGAGGAGGATAGGAGTCTTGGAGTTGTGC
61 AAGAGTTGTGGGCAGGCCTGTTTAGTGGTGTGCAGGGGATGTAGGGGCAGAATCTTGAGG
121 GAAGTGCAGGATGATGGGGAGCCATGGTGGGCGTTGGGGCCTGGGAGGGACCGCCTTCAG
181 GAGAATGAGAACTCAGGGCATTTGGGCAATGGAGATCGGGCTCCCTGGGAAGCCCTCACC
241 ACACCCAGGTAAAAGGTAATTAGGTCTTGATTGCAGGTCAGGAGAGCAGTCCGGGAAGGA
301 AAGTCAGCCAGCGAGGTGGGAGGATTGACAAAACAAATACATTTAGCTTTCCTTGCCCCA
361 TTTCTGACCGCGGGCACCTTTATCCCCGTAAATTATCTCCTGAAGATCTTCTATGTTGAC
421 GCACTGGAGACAGTTCCCCTTTCTCCTTCTGCACCTGCCGCGTTACTCAATCCCCTGTAA
>>>>>>>>>>>>>>>>>>>>
481 TCCCATTGTCCCGACTGCATCATTTCAGAGGTGGTGATTTTAAGGATTTTAACAGGGCTT
<<<<<<<<<<<<<<<<
541 GCCAAAAGAATTATTTTCTTGGCGTTTTGAAAGTAATTACTTAGGCATGGGTACTTTACA
<<<<
601 GAGAAAGCTACTCATCCCGGCTGGCTGCAGAGTTTACAGGGCCCGGGATGAAAACACAGG
661 GCCCAGGTTTCCTGTCCATGAAGCCGGCTCTGCCCCTGATCCTTCTGATGCATCCACCGT

721 GCGTCTGCTCACCTGTCTTGCTTTCTGTTCATTTTCTCTTGTAGTGTGTCCCGGCATGGA
781 TATCCGGAACAACCTCACTAGGTTGCATGAGCTGGAGAATTGCTCTGTCATCGAAGGACA
841 CTTGCAGATACTCTTGATGTTCAAAACGAGGCCCGAAGATTTCCGAGACCTCAGTTTCCC
901 CAAACTCATCATGATCACTGATTACTTGCTGCTCTTCCGGGTCTATGGGCTCGAGAGCCT
961 GAAGGACCTGTTCCCCAACCTCACGGTCATCCGGGGATCACGACTGTTCTTTAACTACGC
1021 GCTGGTCATCTTCGAGATGGTTCACCTCAA
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
829 20 59.98 50.00 4.00 2.00 CATCGAAGGACACTTGCAGA
RIGHT PRIMER
1032 20 59.90 50.00 4.00 2.00 AAGATGACCAGCGCGTAGTT
PRODUCT SIZE: 204, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER
828 20 59.98 50.00 4.00 2.00 TCATCGAAGGACACTTGCAG
RIGHT PRIMER
1032 20 59.90 50.00 4.00 2.00 AAGATGACCAGCGCGTAGTT
PRODUCT SIZE: 205, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER
313 20 59.90 50.00 5.00 3.00 GAGGTGGGAGGATTGACAAA
RIGHT PRIMER
457 20 59.98 55.00 4.00 0.00 CAGGTGCAGAAGGAGAAAGG
PRODUCT SIZE: 145, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00
4 LEFT PRIMER
313 20 59.90 50.00 5.00 3.00 GAGGTGGGAGGATTGACAAA
RIGHT PRIMER
436 20 60.03 55.00 4.00 2.00 GAACTGTCTCCAGTGCGTCA
PRODUCT SIZE: 124, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8161 0 0 0 24 0 1978 4167 0 7 0 171 1814
Right 8182 0 0 0 24 0 1830 4414 0 3 0 167 1744
Pair Stats:
considered 292, unacceptable product size 273, ok 19
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

HRH1 histamine receptor H1 [ Homo sapiens ]

Homo sapiens chromosome 3, GRCh37.p5 Primary Assembly

Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
57 20 60.33 45.00 4.00 2.00 ATTACCCCATGAAGCGTTGA
RIGHT PRIMER
209 20 60.00 55.00 4.00 2.00 ACCTCATACACCGGAAGTGC
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 153, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
1 TTTAATTGTGATAAAATATACATAACAACATGAGTGAGTGCTAAAATATGTGAATTATTA
>>>>
61 CCCCATGAAGCGTTGAAAAAGAAAGACAAGAAGGGGAGAAACGCTCTTCCCAAGTTCCCC
>>>>>>>>>>>>>>>>
121 GGTCCATGCACATACCAAATAACTCTCCTCTATTCTCCACTCCACCATTCATTCAACAAC
181 ATCTATTGAGCACTTCCGGTGTATGAGGTGCTCCTCTAGGCACACGGGATTCCTGCAGAA
<<<<<<<<<<<<<<<<<<<<
241 CAGAGCAGACAAGCATTCCTGCCCTGGTGAGGCCTACATTGTACTTTCCGGCTTCTCCAG
301 GCCCGTGACATCTGCTGTCTCCTGCATTCTCCAGGCCCTGACATCTGCTGTCCCCTAGAT
361 TCTCCAGGCCCATGACATCTGCTGTCCCCTGGCTTCTCCAGGCCCATGACATCTGCTGTC
421 CCCTGGCTTCTCCAGGCTGTGACATCTCCTGTCCCCTGGCTTCTCCAGGCCCGTGACATC
481 TGCTGTCCCCTAGATTCTCCAGGCCCGTGACATCTGCTGTCCCCTGGCTTCTCCAGGCCC
541 ATGACATCTGCTGTCCCCTGGCTTCTCCAGGCCCATGACATCTCCTGTCCCCTGGCTTCT
601 CCAGGCCCATGACATCTGCTGTCCCCTGGCTCCTCCAGACCCACGACATTTGCTGTCCCC
661 TGGATTCTCCAGGCTGTGACATCTGCTGTCCCCTGGCTTCTCCAGGCCCGCAACATCTCC

721 TTCCCCTGGCTTCTCCAGGCTAGTGACATCTGCTGCCCCCTGGCTTCTCCAGGCGCATGA
781 CATCTACTGTCCCTTGGCTTCTCCAGGCCCATGACATCTGCTGACCCCTGCATTCTCCAG
841 ACCAGTGACATCTGCTGGTCCCCTGCATTCTCCAGGCCTGTGACATCTGCTGTTCCCTGG
901 ATTCCCCAGGCCCGTGACATCTGCTGTCCCCTGTATTCTCCAGGCCCGTGACATCTGCTG
961 TCCCCTGGCCTCTCCAGGCCCGTGACATCTGCTGTCCCCTGGCTTCTCCAGGCCCGTGAC
1021 ATCTGCTGTCCCCTGCATTCTCCAGGCCCG
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
57 20 60.33 45.00 4.00 2.00 ATTACCCCATGAAGCGTTGA
RIGHT PRIMER
211 20 60.00 55.00 4.00 2.00 GCACCTCATACACCGGAAGT
PRODUCT SIZE: 155, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER
57 20 60.33 45.00 4.00 2.00 ATTACCCCATGAAGCGTTGA
RIGHT PRIMER
175 20 59.89 45.00 2.00 0.00 TGAATGAATGGTGGAGTGGA
PRODUCT SIZE: 119, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER
57 20 60.33 45.00 4.00 2.00 ATTACCCCATGAAGCGTTGA
RIGHT PRIMER
212 20 60.13 55.00 4.00 0.00 AGCACCTCATACACCGGAAG
PRODUCT SIZE: 156, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER
57 20 60.33 45.00 4.00 2.00 ATTACCCCATGAAGCGTTGA
RIGHT PRIMER
208 20 60.13 55.00 4.00 2.00 CCTCATACACCGGAAGTGCT
PRODUCT SIZE: 152, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 7778 0 0 0 101 0 1153 4973 0 6 0 208 1332
Right 7925 0 0 0 1 0 749 5655 0 6 0 184 1329
Pair Stats:
considered 384, unacceptable product size 371, high end compl 2, ok 11
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

TP63 tumor protein p63 [ Homo sapiens ]

Homo sapiens chromosome 3, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
220 20 59.93 50.00 4.00 0.00 GCCCTGACAAAAGGGATACA
RIGHT PRIMER
468 20 60.07 45.00 6.00 2.00 ATATCGATGCGAACCAAAGC
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 249, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
1 CAATAGAACAAGAGTCCGTCTCCAAAAAAAAAAAAAAAATTAAAGTTATTTTCAGGTTTA
61 GCATAATCCTAAACCATCACTCAAAGCCCCTCTCTCAGAGAAGTCAATTGTCTTACTCAA
121 AAAGTTGGAGATCTCTACCAAAATGTTAGGTTTAAAAGAAAAGAAAATATTCTGGTACAC
181 TGGTAAAATTTCCTTACATGTGACAGCAGTAATATCAGTGCCCTGACAAAAGGGATACAC
>>>>>>>>>>>>>>>>>>>>
241 TGTGGTGATGAGAAAAAGGCACTGACTGAGAGACAGAAAACCTGGATTATGGTCTTGATC
301 ATTTATGCAAACTTAGCCAAGTCACTTGATCTTTCTAAAATTCAGTCTGTGCCTCAGATG
361 AGAAAGAGGATGAGAATGGAGAAACCTACTCTGTTTTCTTAAGGTGAGATGATAGTATTA
421 ATAATTAACGTAGAAAGTTTTTAGAAGGGCTTTGGTTCGCATCGATATATTGTCTGCCAT
<<<<<<<<<<<<<<<<<<<<
481 TCATGAGTGTCCTTGGTTAAGTGATTTTGCTTCTCTTGGATTTTTGTTCACTTTTCTGCA
541 TTCTACCTATAAATTTTACAATCAGGTTTCTACTCACATATCACATGGTAATTATATTTT
601 TATTAAATTTCTTTCTTGTTCACAACCAGGCTTATTTCAGGCCCAGTTCATTTCTTCTAA
661 ATTAGGGCACTTGAACCCTTATCTGAGCAGCTACTGAGATCACCTAAAAAGATGTAAGTT
721 CTTCGTCATGGATACCTGCATCTTAGCTTCTAATTCTTTGAGCCAAATTATATATCCTCA

781 TGTTTATACACAGACTTCCCATTTTAAAAAGTATTTTTTCATATATTTATCTTAGTTGAG
841 CCTCATAACCAAGTTTGTAATCCCCAGTTTTATAGATGAGGAAACTTGAAGATGTAAAGG
901 TCACATGGCTAATAACCAGCCAAGGATGGGGAAGAATCCAGGCCTCCCTCACCCTAATTC
961 AAGTCTCTCCACCATCCTAGTTAGACTGTTTCTCCTCTGGTTTGAGACCAGGGAGGGGCA
1021 TACAGGAGCAGGCAGTGCCTGTGTAGTTTG
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
219 20 59.93 50.00 4.00 2.00 TGCCCTGACAAAAGGGATAC
RIGHT PRIMER
468 20 60.07 45.00 6.00 2.00 ATATCGATGCGAACCAAAGC
PRODUCT SIZE: 250, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER
218 20 59.93 50.00 4.00 2.00 GTGCCCTGACAAAAGGGATA
RIGHT PRIMER
468 20 60.07 45.00 6.00 2.00 ATATCGATGCGAACCAAAGC
PRODUCT SIZE: 251, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
3 LEFT PRIMER
214 20 60.11 50.00 8.00 0.00 ATCAGTGCCCTGACAAAAGG
RIGHT PRIMER
468 20 60.07 45.00 6.00 2.00 ATATCGATGCGAACCAAAGC
PRODUCT SIZE: 255, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER
635 20 60.05 45.00 4.00 0.00 TTTCAGGCCCAGTTCATTTC
RIGHT PRIMER
740 20 59.67 50.00 4.00 0.00 TGCAGGTATCCATGACGAAG
PRODUCT SIZE: 106, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8935 0 0 0 859 0 4679 1065 0 4 18 74 2236
Right 8925 0 0 0 656 0 4642 1232 3 1 6 89 2296
Pair Stats:
considered 135, unacceptable product size 125, ok 10
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

RB1 retinoblastoma 1 [ Homo sapiens ]

Homo sapiens chromosome 13, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
237 20 59.15 45.00 4.00 2.00 TTCTGCCCTCCTTAATTTGG
RIGHT PRIMER
503 20 60.10 50.00 8.00 3.00 GGAGAAGCGCCTCTTTTTCT
SEQUENCE SIZE: 1044
INCLUDED REGION SIZE: 1044
PRODUCT SIZE: 267, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
1 TACCATTCAGATCACTGAATTTATAAAGTACCCATCTAGTACTTGAAAAAGTAAAGTGTT
61 CTGCCAGATCTTAGGTATAGAGGACCCTAACACAGTATATCCCAAGTGCACTTTCTAATG
121 TTTCTGGGTCCTGAAGAATTAAGATACAAATTAATTTTACTCCATAAACAGACTGTTAAT
181 TATAGGAGCCTTAATTTTTTTTTCATAGAGATTTGTCTAATTGCATCTCAAAATTATTCT
>>>>
241 GCCCTCCTTAATTTGGGAAGGTTTGTGTTTTCTCTGGAATGGTACATGTCTTCCATGTAT
>>>>>>>>>>>>>>>>
301 CTTTTGAACTGGCAATTGTCTATTTATCTTTTATTTTTTTAAGTCAGTATGGTCTAACAC
361 TGGCATGTTCAAAGCCACATTATTTCTAGTCCAAAATTACAAGTAATCAAGGGTCATTAT
421 GGGTTAGGCATTAATGTTTCTATCTGATTTTGTGCAAAAGCTTCAAATTAAAACAGCTGC
481 ATTAGAAAAAGAGGCGCTTCTCCCCTCCCCTACACCTAAAGGTGTATTTAAACTATCTTG
<<<<<<<<<<<<<<<<<<<<
541 TGTGATTAACTTATTTAGAGATGCTGTAACTTAAAATAGGGGATATTTAAGGTAGCTTCA
601 GCTAGCTTTTAGGAAAATCACTTTGTCTAACTCAGAATTATTTTTAAAAAGAAATCTGGT
661 CTTGTTAGAAAACAAAATTTTATTTTGTGCTCATTTAAGTTTCAAACTTACTATTTTGAC
721 AGTTATTTTGATAACAATGACACTAGAAAACTTGACTCCATTTCATCATTGTTTCTGCAT

781 GAATATCATACAAATCAGTTAGTTTTTAGGTCAAGGGCTTACTATTTCTGGGTCTTTTGC
841 TACTAAGTTCACATTAGAATTAGTGCCAGAATTTTAGGAACTTCAGAGATCGTGTATTGA
901 GATTTCTTAAATAATGCTTCAGATATTATTGCTTTATTGCTTTTTTGTATTGGTTAAAAC
961 TGTACATTTAAAATTGCTATGTTACTATTTTCTACAATTAATAGTTTGTCTATTTTAAAA
1021 TAAATTAGTTGTTAAGAGTCTTAA
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
362 20 60.90 45.00 4.00 1.00 GGCATGTTCAAAGCCACATT
RIGHT PRIMER
503 20 60.10 50.00 8.00 3.00 GGAGAAGCGCCTCTTTTTCT
PRODUCT SIZE: 142, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER
356 20 58.78 45.00 7.00 2.00 AACACTGGCATGTTCAAAGC
RIGHT PRIMER
503 20 60.10 50.00 8.00 3.00 GGAGAAGCGCCTCTTTTTCT
PRODUCT SIZE: 148, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER
362 19 59.52 47.37 4.00 2.00 GGCATGTTCAAAGCCACAT
RIGHT PRIMER
503 20 60.10 50.00 8.00 3.00 GGAGAAGCGCCTCTTTTTCT
PRODUCT SIZE: 142, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
4 LEFT PRIMER
236 21 59.45 42.86 4.00 2.00 ATTCTGCCCTCCTTAATTTGG
RIGHT PRIMER
503 20 60.10 50.00 8.00 3.00 GGAGAAGCGCCTCTTTTTCT
PRODUCT SIZE: 268, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8789 0 0 0 688 0 5698 474 0 3 59 64 1803
Right 8825 0 0 0 1129 0 5414 496 0 1 54 57 1674
Pair Stats:
considered 68, unacceptable product size 63, ok 5
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

TNF tumor necrosis factor [ Homo sapiens ]

Homo sapiens chromosome 6, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
395 20 60.10 55.00 6.00 2.00 GCACAGGCCTTAGTGGGATA
RIGHT PRIMER
519 20 60.11 55.00 2.00 0.00 CGGTTCACTCTCCACATCCT
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 125, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
1 TAATCAGCCCTCTGGCCCAGGCAGTCAGTAAGTGTCTCCAAACCTCTTTCCTAATTCTGG
61 GTTTGGGTTTGGGGGTAGGGTTAGTACCGGTATGGAAGCAGTGGGGGAAATTTAAAGTTT
121 TGGTCTTGGGGGAGGATGGATGGAGGTGAAAGTAGGGGGGTATTTTCTAGGAAGTTTAAG
181 GGTCTCAGCTTTTTCTTTTCTCTCTCCTCTTCAGGATCATCTTCTCGAACCCCGAGTGAC
241 AAGCCTGTAGCCCATGTTGTAGGTAAGAGCTCTGAGGATGTGTCTTGGAACTTGGAGGGC
301 TAGGATTTGGGGATTGAAGCCCGGCTGATGGTAGGCAGAACTTGGAGACAATGTGAGAAG
361 GACTCGCTGAGCTCAAGGGAAGGGTGGAGGAACAGCACAGGCCTTAGTGGGATACTCAGA
>>>>>>>>>>>>>>>>>>>>
421 ACGTCATGGCCAGGTGGGATGTGGGATGACAGACAGAGAGGACAGGAACCGGATGTGGGG
481 TGGGCAGAGCTCGAGGGCCAGGATGTGGAGAGTGAACCGACATGGCCACACTGACTCTCC
<<<<<<<<<<<<<<<<<<<<
541 TCTCCCTCTCTCCCTCCCTCCAGCAAACCCTCAAGCTGAGGGGCAGCTCCAGTGGCTGAA
601 CCGCCGGGCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGAGATAACCAGCTGGTGGT
661 GCCATCAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTCAAGGGCCAAGGCTGCCC
721 CTCCACCCATGTGCTCCTCACCCACACCATCAGCCGCATCGCCGTCTCCTACCAGACCAA

781 GGTCAACCTCCTCTCTGCCATCAAGAGCCCCTGCCAGAGGGAGACCCCAGAGGGGGCTGA
841 GGCCAAGCCCTGGTATGAGCCCATCTATCTGGGAGGGGTCTTCCAGCTGGAGAAGGGTGA
901 CCGACTCAGCGCTGAGATCAATCGGCCCGACTATCTCGACTTTGCCGAGTCTGGGCAGGT
961 CTACTTTGGGATCATTGCCCTGTGAGGAGGACGAACATCCAACCTTCCCAAACGCCTCCC
1021 CTGCCCCAATCCCTTTATTACCCCCTCCTT
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
238 20 60.14 55.00 6.00 2.00 GACAAGCCTGTAGCCCATGT
RIGHT PRIMER
474 20 60.07 55.00 4.00 0.00 ATCCGGTTCCTGTCCTCTCT
PRODUCT SIZE: 237, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER
865 20 59.89 60.00 3.00 3.00 CTATCTGGGAGGGGTCTTCC
RIGHT PRIMER
998 20 60.11 55.00 3.00 3.00 ATGTTCGTCCTCCTCACAGG
PRODUCT SIZE: 134, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
3 LEFT PRIMER
132 20 59.86 50.00 2.00 0.00 GAGGATGGATGGAGGTGAAA
RIGHT PRIMER
414 20 60.10 55.00 6.00 2.00 TATCCCACTAAGGCCTGTGC
PRODUCT SIZE: 283, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
4 LEFT PRIMER
238 20 60.14 55.00 6.00 2.00 GACAAGCCTGTAGCCCATGT
RIGHT PRIMER
414 20 60.10 55.00 6.00 2.00 TATCCCACTAAGGCCTGTGC
PRODUCT SIZE: 177, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 7882 0 0 0 0 0 1267 4879 0 5 22 180 1529
Right 7828 0 0 0 0 0 1139 5020 1 0 22 189 1457
Pair Stats:
considered 51, unacceptable product size 39, ok 12
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

PVRL1 poliovirus receptor-related 1 (herpesvirus entry mediator C) [ Homo sapiens ]

Homo sapiens chromosome 11, GRCh37.p5 Primary Assembly

Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
75 20 60.01 60.00 3.00 1.00 GCCCTTACACAGAGGAGCAG
RIGHT PRIMER
371 20 59.94 45.00 3.00 1.00 TGGAAATGTGAGTTGGGACA
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 297, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
1 GCACCGAGGAGATGGACCGGACAGAATCTGTGTGTGCTTCCGTGGGCACAGGCCCCAGAG
61 AACCCCTTTCAGAGGCCCTTACACAGAGGAGCAGTTTCATGGGGTGGGCCCAGTGGTCAC
>>>>>>>>>>>>>>>>>>>>
121 AAAATGAAGCTCCCTCCCTTCTCCCTCTGGGGTAATGACAAGGTGGGGAGAGGCCCAGGG
181 TGCAGCCTCCACCAGACTGAAAAAAAACAGATGAGGCAGCCTCTATCCCTGTGGGTGTCG
241 GGAGGAGACAGGCCAGGCCTCTCCAGCTGGGACCCAGCCTCTCGCCACTCCCAAGAGCAG
301 GACAGGGAGGCTGCCAGGGTGTAGGATGTCCATCCCAGGGTGCAGGGCGTCTGTCCCAAC
<<<<<<<<<
361 TCACATTTCCAGTGAAGCCCGGGCTCATCCCTTGCAGGTGAGGGTCACAGTCTGCCCATC
<<<<<<<<<<<
421 AGTCCTGGAGTTCTTCAGACCCAGAATTGCGGGCCTTGAAGGCCAGTGTCCTGGAAGGGA
481 GGCAGGTGTGAGCCTGCAAGTGTGCATGCCCAGCCATGGTATGGACATGTGTCTCTGGGC
541 ATGTAAATGTGAACCAGTGTGAACAGGCTCCGTCTGCATGCTGAGTGTGCATGTGGGAGC
601 CCGTGGCTGTGCCGTGGCAACGTGCCATTCTCTGAGCCAGCGAATGGCAGTGTGTTGGGA
661 GGTCTGAGAAGGCAGCTGCATCCGTGCCTCTGGGAGGATTCGGTTCTCCCCCAGCTTGCC
721 GAGGCCCTGCCTGATGGTCTGACACGAGGCACAGCTGCTGCAGCTGCAGATGGACAGAAG

781 GGCTTCCCAGAGGTGGACCCCAGGCCTCCCCACTCTCCCTGTGGCTGGCTGCACTGCATG
841 CTGGGGGGTGTAGTTCTTGCAGCTTCCAGGCCTAATCTGATGCCGGAGCATTTCCTGCCT
901 GAGGAAGCGCCAGGCATTGGTTTCGGAGGCAAACCCAAACATTCTCTTTGACCCCAAACC
961 TCCAGATCCTAGATCCAGACTGTAAGCCCTAACACTTCACTCCACCTCAGATCTATCCAA
1021 AGCCCCCAGCACCAGCCCACCCACCTCAGT
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
141 20 59.92 55.00 4.00 3.00 CTCCCTCTGGGGTAATGACA
RIGHT PRIMER
371 20 59.94 45.00 3.00 1.00 TGGAAATGTGAGTTGGGACA
PRODUCT SIZE: 231, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER
352 20 59.94 45.00 3.00 0.00 TGTCCCAACTCACATTTCCA
RIGHT PRIMER
575 20 59.91 55.00 4.00 2.00 AGACGGAGCCTGTTCACACT
PRODUCT SIZE: 224, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER
184 20 59.84 50.00 5.00 0.00 AGCCTCCACCAGACTGAAAA
RIGHT PRIMER
371 20 59.94 45.00 3.00 1.00 TGGAAATGTGAGTTGGGACA
PRODUCT SIZE: 188, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER
352 20 59.94 45.00 3.00 0.00 TGTCCCAACTCACATTTCCA
RIGHT PRIMER
571 20 60.16 55.00 4.00 3.00 GGAGCCTGTTCACACTGGTT
PRODUCT SIZE: 220, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 7570 0 0 0 0 0 517 5899 2 2 42 193 915
Right 7660 0 0 0 1 0 663 5752 1 1 41 182 1019
Pair Stats:
considered 72, unacceptable product size 61, ok 11
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

ESR1 estrogen receptor 1 [ Homo sapiens ]

Homo sapiens chromosome 6, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
859 20 60.02 55.00 3.00 0.00 CAGTCACAAGACAGGGCAGA
RIGHT PRIMER
1004 20 59.96 50.00 4.00 2.00 AATCATGTTGGCTCCTACGG
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 146, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
1 ATGGTCATAACAGCCTCCTGTCTACCGACTCAGAACGGATTTTACCAAAACTGAAAATGC
61 AGGCTCCATGCTCAGAAGCTCTTTAACAGGCTCGAAAGGTCCATGCTCCTTTCTCCTGCC
121 CATTCTATAGCATAAGAAGACAGTCTCTGAGTGATAATCTTCTCTTCAAGTAGGTACTCC
181 TATTTCTTCTCAATTTATTTTTTCCTTTTTGATATAATGTGCTACTGTTTACAAGCATAT
241 TGTAACTTCAGAGCTTACCTCTCATCTTTAAAAAATGTTCATTTTTTTGTCTTTCTGCTC
301 CAAGGATATTTTGCAAAGTTACTGGCAAGTATTCCTGGGATGATAAAATGTGAAATCTAA
361 ACTTGGTACAGTGGAAATTCACTTCTAGAATAATATTTAGCTGAGGCAGAGGGCAATCCG
421 ACTACCCTTTTCTTAGTACAGCACACACAGGCTGCCTGTCTGTTCCAGATAACATAAATG
481 TATTGGATCTAGCACTAGCTAGGAGACACTGTATTGTTGAACTGTGTTAGAATTTTATAA
541 GCTCTTAATTGGACAAATCTCAGAGTAGCATGAACACACTACCTGTTTTCTGAATCTTTG
601 GAGCCATAACTTACGTGAGTTTGAACTAAGCGATGTGAATAAGCCATTATTTGTTTCCTA
661 AAGGCAGTCAAGTTTTCTGAAAAGCTACACATTTAGCAGCAAAAGAACGAGCCCCTCTGT

721 CTTGAAATGGGCCTCTGATTTTAAGCAAGCTCTTTTGAGTCCTGGTGTCCCATTTTCTCA
781 GTTCCTTTTTGCCTCACAATGGCACATACATAATGACTCCACCACATATAGCAGTGGGCT
841 ACTCGGGTAATGATGTGGCAGTCACAAGACAGGGCAGAATACTTTCATTTTGGTTAGAGG
>>>>>>>>>>>>>>>>>>>>
901 AATGCCACATGTCTTAGGAAATGCTCGCTGAACTGTAAGTTTCATACTTTGTCAAGAAGA
961 TAACCAGTATTCTCTCAAACAAGTCCGTAGGAGCCAACATGATTAAAAGATTTTAAAGCA
<<<<<<<<<<<<<<<<<<<<
1021 ATTTACTCGATAGAAGGGTTGGGCTTAATC
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
834 20 59.96 55.00 6.00 2.00 GTGGGCTACTCGGGTAATGA
RIGHT PRIMER
1004 20 59.96 50.00 4.00 2.00 AATCATGTTGGCTCCTACGG
PRODUCT SIZE: 171, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER
760 20 59.90 50.00 3.00 0.00 TCCTGGTGTCCCATTTTCTC
RIGHT PRIMER
878 20 60.02 55.00 3.00 2.00 TCTGCCCTGTCTTGTGACTG
PRODUCT SIZE: 119, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER
760 20 59.90 50.00 3.00 0.00 TCCTGGTGTCCCATTTTCTC
RIGHT PRIMER
1004 20 59.96 50.00 4.00 2.00 AATCATGTTGGCTCCTACGG
PRODUCT SIZE: 245, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER
859 20 60.02 55.00 3.00 0.00 CAGTCACAAGACAGGGCAGA
RIGHT PRIMER
1001 20 60.13 55.00 4.00 1.00 CATGTTGGCTCCTACGGACT
PRODUCT SIZE: 143, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8522 0 0 0 247 0 4395 1551 0 0 45 99 2185
Right 8643 0 0 0 347 0 4555 1464 0 0 44 97 2136
Pair Stats:
considered 22, unacceptable product size 13, ok 9
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

PGR progesterone receptor [ Homo sapiens ]

Homo sapiens chromosome 11, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
651 20 59.98 40.00 4.00 0.00 GCCATTGCTTTTGGTGTTTT
RIGHT PRIMER
855 20 59.97 50.00 3.00 2.00 GGTGTTGGGAAAACTGGCTA
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 205, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
1 CACATTTGCTTAATCCAGTCTGTCATTGATGGACATTTGGCTTGGTTCCAAGTCTTTGCT
61 ATTGTGAATAGTGCTTCAGTAAACATATGTGTGCCTGTGTCTTTATAGTAGAATGATTTA
121 TAATCCTCTGGGTTTATACCCAGTAATGGGATTGCTGGGTCAAATGGTAATTCTAGTTCT
181 AGATCCTTGAGGAATTGCTGCACTGTCTTCCACAATGGTTGAACTAATTTACACTCCCAC
241 CAACAGTGTAAAAGCTTTCCTATTTCTCCACATCCTCTCCAGCATCTGTTGTTTCCTGAC
301 TTTTTAATGGTTGCCATTCCAACTGCCATGAGATGGTATCTTATTGTGATTTTGATTTGC
361 ATTTCTCTAATGACTAGTGATGATGAGCATTTTTTCATGTCTGTCGGCTGCATAAATGTC
421 TTTTTTTGAGAAGTGTCTGCTCATATCCTTTGCCCAATTTATGAGGTTGTTTTTTCCTTG
481 TAAATTTAAGTTCCTTCTAGATTCTGGATATTAGTCCTTTGTCAGATGGATAGATTGCAA
541 AAATTTTCTTCCATTCTGTAGGTTGCCTGTTCCCTCTGATGATAGTTTCTTTTGCTGTGC
601 AGAAGTTCTTTAGTTTAATTAGATCCCATTTGTCAATTTTGGCTTTTGTTGCCATTGCTT
>>>>>>>>>>
661 TTGGTGTTTTAGACATGAAGTCTCTGCCTACACCTATCTCCTGAATAGTGTTGCCTAGGT
>>>>>>>>>>

721 TTTCTTCTAGGATTTTTATGGTTTGCAGTCTTACATTTAAGTCTTTAATCCATCTTGAGT
781 TAATTTTTGTATAAGGTGTTAGGAAGGGGTCCAGTTTCAGTTTTCTGCTTATGGCTAGCC
<<<<<
841 AGTTTTCCCAACACCATTTATTAAACAGGGAATCCTTTCCCCATTGCTTGTTTGAGTCAA
<<<<<<<<<<<<<<<
901 GTTTGTCAAAGATCAGATGGTTGTATATGTGTGGTGTTATTTCTGAGGTCTCTTTTCTGT
961 CCATTGGTCTATATATCCGTTTTGGTACCAGTGCCATGCTGTTTTGGTTACTGCAGCCTT
1021 GTAGTATAGTTTGAAGTCAGGTAGTGTGAT
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
559 20 60.25 55.00 3.00 1.00 TAGGTTGCCTGTTCCCTCTG
RIGHT PRIMER
855 20 59.97 50.00 3.00 2.00 GGTGTTGGGAAAACTGGCTA
PRODUCT SIZE: 297, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
2 LEFT PRIMER
642 20 60.25 40.00 4.00 0.00 GCTTTTGTTGCCATTGCTTT
RIGHT PRIMER
855 20 59.97 50.00 3.00 2.00 GGTGTTGGGAAAACTGGCTA
PRODUCT SIZE: 214, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
3 LEFT PRIMER
644 20 59.72 35.00 4.00 0.00 TTTTGTTGCCATTGCTTTTG
RIGHT PRIMER
855 20 59.97 50.00 3.00 2.00 GGTGTTGGGAAAACTGGCTA
PRODUCT SIZE: 212, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER
651 20 59.98 40.00 4.00 0.00 GCCATTGCTTTTGGTGTTTT
RIGHT PRIMER
858 20 59.69 45.00 3.00 0.00 AATGGTGTTGGGAAAACTGG
PRODUCT SIZE: 208, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 8611 0 0 0 93 0 4443 1420 0 5 65 72 2513
Right 8545 0 0 0 90 0 4473 1356 0 4 60 82 2480
Pair Stats:
considered 126, unacceptable product size 114, high any compl 1, high end compl 1, ok 10
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

HPV225_r3 16S ribosomal RNA [ Helicobacter pylori v225d ]

Helicobacter pylori v225d chromosome, complete genome
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
338 20 59.96 60.00 2.00 0.00 GTGTGGGAGAGGTAGGTGGA
RIGHT PRIMER
500 20 59.99 55.00 3.00 3.00 GTTTAGGGCGTGGACTACCA
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 163, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
1 CCTGAGAGGGTGAACGGACACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCA
61 GCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAAGCAGCAACGCCGCGTGGAGGATG
121 AAGGTTTTAGGATTGTAAACTCCTTTTGTTAGAGAAGATAATGACGGTATCTAACGAATA
181 AGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCGG
241 AATCACTGGGCGTAAAGAGCGCGTAGGCGGGATAGTCAGTCAGGTGTGAAATCCTATGGC
301 TTAACCATAGAACTGCATTTGAAACTACTATTCTAGAGTGTGGGAGAGGTAGGTGGAATT
>>>>>>>>>>>>>>>>>>>>
361 CTTGGTGTAGGGGTAAAATCCGTAGAGATCAAGAGGAATACTCATTGCGAAGGCGACCTG
421 CTGGAACATTACTGACGCTGATTGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCC
481 TGGTAGTCCACGCCCTAAACGATGGATGCTAGTTGTTGGAGGGCTTAGTCTCTCCAGTAA
<<<<<<<<<<<<<<<<<<<<
541 TGCAGCTAACGCATTAAGCATCCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAG
601 GAATAGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGATACACGAAG
661 AACCTTACCTAGGCTTGACATTGAGAGAATCCGCTAGAAATAGTGGAGTGTCTGGCTTGC

721 CAGACCTTGAAAACAGGTGCTGCACGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTT
781 AAGTCCCGCAACGAGCGCAACCCCCTTTCTTAGTTGCTAACAGGTCATGCTGAGAACTCT
841 AAGGATACTGCCTCCGTAAGGAGGAGGAAGGTGGGGACGACGTCAAGTCATCATGGCCCT
901 TACGCCTAGGGCTACACACGTGCTACAATGGGGTGCACAAAGAGAAGCGATACTGCGAAG
961 TGGAGCCAATCTTCAAAACACCTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTGCATGA
1021 AGCTGGAATCGCTAGTAATCGCAAATCAGC
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
338 20 59.96 60.00 2.00 0.00 GTGTGGGAGAGGTAGGTGGA
RIGHT PRIMER
553 20 60.04 50.00 4.00 2.00 TGCGTTAGCTGCATTACTGG
PRODUCT SIZE: 216, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER
239 20 60.07 50.00 3.00 0.00 GGAATCACTGGGCGTAAAGA
RIGHT PRIMER
500 20 59.99 55.00 3.00 3.00 GTTTAGGGCGTGGACTACCA
PRODUCT SIZE: 262, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
3 LEFT PRIMER
755 20 60.05 55.00 5.00 2.00 CAGCTCGTGTCGTGAGATGT
RIGHT PRIMER
943 20 59.96 45.00 6.00 2.00 TCTTTGTGCACCCCATTGTA
PRODUCT SIZE: 189, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER
755 20 60.05 55.00 5.00 2.00 CAGCTCGTGTCGTGAGATGT
RIGHT PRIMER
904 20 59.96 50.00 5.00 1.00 CGTAAGGGCCATGATGACTT
PRODUCT SIZE: 150, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8192 0 0 0 1 0 2354 3907 0 1 0 175 1754
Right 8287 0 0 0 1 0 2404 3916 1 1 0 161 1803
Pair Stats:
considered 87, unacceptable product size 76, high end compl 1, ok 10
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

LHB luteinizing hormone beta polypeptide [ Homo sapiens ]

Homo sapiens chromosome 19, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
247 20 59.92 55.00 5.00 3.00 TGGTGGGTCCTGAATAGGAG
RIGHT PRIMER
525 20 60.01 55.00 3.00 2.00 GCACAGATGGTGGTGTTGAC
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 279, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
1 GCACCAAGGATGGAGATGCTCCAGGTAAGACTACAGGGCCCCTGGGCACCTTCCACCTCC
61 TTCCAGGCCATCACTGGCATGAGAAGGGGCAGACCCGTGTGAGCTGTGGAAGGAGGCCTC
121 TTTCTGGAGGGGCATGACCCCCAGTAAGCTTCAGGTGGGGCAGTTCCTGAGGGTGGGGAT
181 CTGAAATGTTGGGGCATCTCAGGTCCTCTGGGCTGTGGGGTGGGCTCTGAAAGGCAGGTG
241 TCCGGGTGGTGGGTCCTGAATAGGAGATGCCGGGAAGGGTCTCTGGGTCTTTGTGGGTGG
>>>>>>>>>>>>>>>>>>>>
301 TGTACCACGCGGGATGGGAAGGCCAGGACTCGGGGCTGCGGTCTCAGACCTGGGTGAAGC
361 AGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAGCATGGGCGGGGCATGGGC
421 ATCCAGGGAGCCGCTTCGGCCATGGTGCCACCCCATCAATGCCATCCTGGCTGTCGAGAA
481 GGAGGGCTGCCCAGTGTGCATCACCGTCAACACCACCATCTGTGCCGGCTACTGCCCCAC
<<<<<<<<<<<<<<<<<<<<
541 CATGGTGAGCTGCCTGGGGCCAGGGGCAGATGCTGCCACCTCAGGGCCAGACCCACAGAG
601 GCAGCGGGGGAGGAAGGGTGGTCTGCCTCTCTGGCCTGCGGTTGGGGAATGGGGTGTGGG
661 AAGGCAGGAACAGAGGGCTTCCTGGGCTCCTGAGTCCAGGACCTGTGGGGTCAGCTTGGG
721 AGCTCAGCTGAGGCGCTGGCCTCAGGCACATGCTCATTCCCCCACTCACACGGCCTCCAG

781 ATGCGCGTGCTGCAGGCGGTCCTGCCGCCCCTGCCTCAGGTGGTGTGCACCTACCGTGAT
841 GTGCGCTTCGAGTCCATCCGGCTCCCTGGCTGCCCGCGTGGTGTGGACCCCGTGGTCTCC
901 TTCCCTGTGGCTCTCAGCTGTCGCTGTGGACCCTGCCGCCGCAGCACCTCTGACTGTGGG
961 GGTCCCAAAGACCACCCCTTGACCTGTGACCACCCCCAACTCTCAGGCCTCCTCTTCCTC
1021 TAAAGACCCTCCCCGCAGCCTTCCAAGTCC
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
8 20 60.03 55.00 8.00 2.00 GGATGGAGATGCTCCAGGTA
RIGHT PRIMER
266 20 59.92 55.00 5.00 0.00 CTCCTATTCAGGACCCACCA
PRODUCT SIZE: 259, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER
341 20 59.84 60.00 7.00 2.00 GTCTCAGACCTGGGTGAAGC
RIGHT PRIMER
525 20 60.01 55.00 3.00 2.00 GCACAGATGGTGGTGTTGAC
PRODUCT SIZE: 185, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
3 LEFT PRIMER
352 20 60.16 55.00 3.00 0.00 GGGTGAAGCAGTGTCCTTGT
RIGHT PRIMER
525 20 60.01 55.00 3.00 2.00 GCACAGATGGTGGTGTTGAC
PRODUCT SIZE: 174, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
4 LEFT PRIMER
8 20 60.03 55.00 8.00 2.00 GGATGGAGATGCTCCAGGTA
RIGHT PRIMER
307 20 59.85 50.00 6.00 0.00 TGGTACACCACCCACAAAGA
PRODUCT SIZE: 300, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8034 0 0 0 83 0 177 6923 1 3 0 168 679
Right 8060 0 0 0 83 0 165 6934 1 0 0 166 711
Pair Stats:
considered 49, unacceptable product size 42, ok 7
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

OCA2 oculocutaneous albinism II [ Homo sapiens ]

Homo sapiens chromosome 15, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
587 20 59.99 55.00 3.00 2.00 GAGAAGAACGCAACCTCCAG
RIGHT PRIMER
858 20 60.00 55.00 7.00 0.00 AACCACACTCAGTGGGAAGG
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 272, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
1 TTTGCAGAACCAGGCAGGGGCCAGGGGCTCAGGACAAGTGCCCGGTGCTCCCTTCCCATT
61 GGGCGAATCAGAGCCTGGGGCCCGGCGGTGAAGCTCCCCAGGTGACTCTAATGTGCAGTG
121 CACTTTGAGGAGCACTACTTAGACCAATGTGACAGTCTACAATGTGTAGATTTAGGGTGA
181 GTGATTCTGAGGAAAAGAAACCCGAGGCTGTTAGCAGTTGTGGGCAGCTGCTGTCTACCT
241 AAACCAGCTGCGGTTTGCTTGCTTGGTGAGCCTGAGCTTCGCGGGGCGGGCATGGCATCT
301 GCCATCCAGGACCCTGGGACGGGGCCTGCCAGGGCAAGGAGCTGAGCATTGAACGCATCT
361 GGGGATAACTATCTCTCATAGAAGAACTAATGAGGATTGAACCTGAACACAGAGATGAAA
421 GAGCTGAGTAGACTGCAAAGAATTGCACAAAAACTGCTTGTTCTGCAAATTTAGTTTACA
481 ACAATTTGAGTGCAGTTCACATTGGTGTAATGTGATTTAGGATCATAAGTCCCTAAAGTT
541 TCATTACACTGATGAAAAGCAAATGCCTATACTGTTCTTGTTTTATGAGAAGAACGCAAC
>>>>>>>>>>>>>>
601 CTCCAGCCCCTGGAGCAGCTGAGATCTGAGGATATCAGGAATACCAGGAATGGGGAGGTC
>>>>>>
661 CTGGCTGTCCCTGGAAACCCAGCGGCATGACACCTGTGTCACCTGGGTCCCCGTTTCTAT
721 GCTGGAGTGGCAGGCAGCAAGGAAATATTTTGAGTTTGAGGCCATATGCATTTTGGAAAG

781 ATCACTGTGGCTGGGGGGTTGGGAGCAGCTGGGGAGTTGAAGAAAGCCACTGCCAAGGCC
<<
841 TTCCCACTGAGTGTGGTTGCCAGGCCGGGGCAGGGAAGCACTGGTGAGCTGCAGGGGGGA
<<<<<<<<<<<<<<<<<<
901 TTCCCAGTTGGTGATGGGTGATGGATGGTGGGATCAGTGGGCCCCTGGGAAAGGCCAGGT
961 GTGGCTTCAGCACTCTGGGAGTTTTGGGTACCTTTATGTCTAGGGACACAAAGAGGTAGA
1021 TGGACACAGTGGTTTGAGCTGCAGAAGAGA
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
641 20 60.01 55.00 5.00 2.00 ATACCAGGAATGGGGAGGTC
RIGHT PRIMER
858 20 60.00 55.00 7.00 0.00 AACCACACTCAGTGGGAAGG
PRODUCT SIZE: 218, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER
587 20 59.99 55.00 3.00 2.00 GAGAAGAACGCAACCTCCAG
RIGHT PRIMER
793 20 59.83 50.00 5.00 0.00 CAGCCACAGTGATCTTTCCA
PRODUCT SIZE: 207, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
3 LEFT PRIMER
587 20 59.99 55.00 3.00 2.00 GAGAAGAACGCAACCTCCAG
RIGHT PRIMER
770 20 60.22 45.00 6.00 1.00 TGCATATGGCCTCAAACTCA
PRODUCT SIZE: 184, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
4 LEFT PRIMER
641 20 60.01 55.00 5.00 2.00 ATACCAGGAATGGGGAGGTC
RIGHT PRIMER
770 20 60.22 45.00 6.00 1.00 TGCATATGGCCTCAAACTCA
PRODUCT SIZE: 130, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8229 0 0 0 94 0 2210 4173 2 5 41 122 1582
Right 8246 0 0 0 57 0 2469 3737 0 0 43 123 1817
Pair Stats:
considered 79, unacceptable product size 65, high end compl 3, ok 11
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok

EFNA5 ephrin-A5 [ Homo sapiens ]

Homo sapiens chromosome 5, GRCh37.p5 Primary Assembly
Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO
start len
tm gc% any 3' seq
LEFT PRIMER
48 20 60.11 50.00 2.00 2.00 GGGGTGCGAGTTTGTCTTTA
RIGHT PRIMER
330 20 59.83 50.00 2.00 0.00 CATCCAGAGCACCAGAAACA
SEQUENCE SIZE: 1050
INCLUDED REGION SIZE: 1050
PRODUCT SIZE: 283, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
1 GCTTCTCTCCATCTTGTGATTCCTTTTTCCTCCTGAACCCTCCAGTGGGGGTGCGAGTTT
>>>>>>>>>>>>>
61 GTCTTTATCACCCCCCATCCCACCGCCTTCTTTTCTTCTCGCTCTCCTACCCCTCCCCAG
>>>>>>>
121 CTTGGTGGGCGCCTCTTTCCTTTCTCGCCCACTTTCATTTTTATTTATTCATATTTATTT
181 GGCGCCCGCTCTCTCTCTGTCCCTTTGCCTGCCTCCCTCCCTCCGGATCCCCGCTCTCTC
241 CCCGGAGTGGCGCGTCGGGGGCTCCGCCGCTGGCCAGGCGTGATGTTGCACGTGGAGATG
301 TTGACGCTGGTGTTTCTGGTGCTCTGGATGTGTGTGTTCAGCCAGGACCCGGGCTCCAAG
<<<<<<<<<<<<<<<<<<<<
361 GCCGTCGCCGACCGCTACGCTGTCTACTGGAACAGCAGCAACCCCAGGTAAGCCGATAAC
421 AGGCGGAGAGCCAGGGCTACCCGGGCCTCGGGGCCCTGCGGAAGGAGCGGCGGCGGGGAT
481 CGGCGCGCACCAACCGCTGGCGGCGCGCACCCTCGGAGCCTGCGTCGCCCGGGCGCTGAT
541 GGCTTGAGGCGTCGGCGGGAAGCGCTCGCGTCGCGCCCCTCTTTGTTAAGCCGCCGCGGA
601 AAGGCCAGGTACCCCTGGGAGTCCCCGGGGCTGCCTAGACCAACTGCTTCTGCGGGGCGC
661 CGGGAGACTCGGAAAAGGGCGGGAGCCGGGGAGTAGGGACTTCTCTGCTTCCCAGCCCCG
721 GCATCTGGCTGGCATCCGGTTCCCGGCGCGGGGAGCCCCCAGTGTGCGCAGCCGGGAGCT

781 CCTTCCTGGAGCGTGGGTCCGCAGGGACTGCGGAGAGAACCCGCCAAACATCCCCCAAAG
841 TTTTTTTTTTTTTTTTTTTTTTTTTAATTTTAATTTTCCCTTCTCTTTTCCCGGTGCGCG
901 CAGAGAGAGCGCACGGGCTGGGGGAAGTGAGGGGGGCTGGGAAGCGGAAGCTTTAGGTTT
961 TTATTTTCCTCTTCAACTGCGAGGAAATCATTCGGCCCCTGAGTCAGTCTGGGCCCGGCG
1021 CGCCTGGCAGTGGAGAGGATTCTCCCATCT
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len
tm

gc% any

3' seq

1 LEFT PRIMER
48 20 60.11 50.00 2.00 2.00 GGGGTGCGAGTTTGTCTTTA
RIGHT PRIMER
332 20 59.83 50.00 2.00 0.00 CACATCCAGAGCACCAGAAA
PRODUCT SIZE: 285, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
2 LEFT PRIMER
48 20 60.11 50.00 2.00 2.00 GGGGTGCGAGTTTGTCTTTA
RIGHT PRIMER
335 20 59.74 55.00 2.00 1.00 ACACACATCCAGAGCACCAG
PRODUCT SIZE: 288, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER
48 20 60.11 50.00 2.00 2.00 GGGGTGCGAGTTTGTCTTTA
RIGHT PRIMER
162 20 59.69 40.00 2.00 0.00 AAAAATGAAAGTGGGCGAGA
PRODUCT SIZE: 115, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
4 LEFT PRIMER
48 20 60.11 50.00 2.00 2.00 GGGGTGCGAGTTTGTCTTTA
RIGHT PRIMER
161 20 59.69 40.00 2.00 0.00 AAAATGAAAGTGGGCGAGAA
PRODUCT SIZE: 114, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
Statistics
con too in in
no tm tm high high
high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab
Left 8141 0 0 0 1459 0 469 5475 0 4 57 115 562
Right 8292 0 0 0 1536 0 453 5594 0 0 36 117 556
Pair Stats:
considered 542, unacceptable product size 501, high end compl 8, ok 33
primer3 release 1.1.4
(primer3_results.cgi release 0.4.0)

ok