APPENDIX 12

RR CANOLA TESTING REQUIREMENTS - MONSANTO QA GUIDELINES (and internal PAC requirements)

Test Required Method Details of Test Comments
Gateway PCR
All plants tested for presence of
unintended events (including RT200)
900 seeds (<0.5% AP
@ 99% conf.)
PCR
Testing for presence of unintended
events (including RT200)
Only required if generation
wasn't gateway'd
Trait Testing
Roll towel;
Spray trays;
RuR test strips;
PCR
To confirm presence of Roundup Ready
Trait in line
max 3% impurity level @ 95%
confidence level
600 seeds (<0.5% AP
@ 95% conf.)
PCR
Testing for presence of unintended
events (including RT200)
Trait Testing
Roll towel;
Spray trays;
RuR test strips;
PCR
To confirm presence of Roundup Ready
Trait in line
max 3% impurity level @ 95%
confidence level
1400 seeds PCR
Testing for presence of unintended
events (not specific for RT200)
Inhouse testing requirement only
(not required by Monsanto)
Trait Testing
Roll towel;
Spray trays;
RuR test strips;
PCR
To confirm presence of Roundup Ready
Trait in line
max 15% impurity level @ 95%
confidence level
Gateway PCR
All plants tested for presence of
unintended events
900 seeds (<0.5% AP
@ 99% conf.)
PCR
Testing for presence of unintended
events
Only required if generation
wasn't gateway'd
Basic nil required
Testing required only if
contamination expected
*Monsanto requirement for unintended events includes RT200 only, internal requirements require additional screening as appropriate
REPORTING DATES MAIN SEASON PRODUCTION CONTRA PRODUCTION
STAGE 1 August 1 March 1
STAGE 2 August 1 March 1
STAGE 3 August 1 March 1
STAGE 4 March 1 (prior to planting) March 1 (prior to planting)
R
O
U
N
D
U
P

R
E
A
D
Y
C
O
N
V
E
N
T
I
O
N
A
L
Production Stage
Prebasic
Basic
Commercial
Prebasic
QCLM-4 Genetic Purity Testing
Copyright Pacific Seeds Pty Ltd
Last updated March 2014
S:\qc\GENETIC PURITY\CANOLA\ROUNDUP READY\
Roundup Ready - Testing Summary.xls