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  • Spectrophotometry Lab

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    meeechers
    17 views23,309 pages
    The document contains graphs and tables showing the relationship between wavelength, absorbance, and concentration for the compounds Bromothymol Blue and Thymol Blue. Specifically, it shows absorbance measurements across wavelengths from 350-700 nm for the individual compounds and their sum, and it shows increasing absorbance at 618 nm with higher concentrations of Bromothymol Blue. The data and graphs indicate the absorbance properties and behaviors of these compounds at different wavelengths and concentrations.

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    Lab

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    The document contains graphs and tables showing the relationship between wavelength, absorbance, and concentration for the compounds Bromothymol Blue and Thymol Blue. Specifically, it shows absorbance measurements across wavelengths from 350-700 nm for the individual compounds and their sum, and it shows increasing absorbance at 618 nm with higher concentrations of Bromothymol Blue. The data and graphs indicate the absorbance properties and behaviors of these compounds at different wavelengths and concentrations.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    17 views23,309 pages

    Spectrophotometry Lab

    Uploaded by

    meeechers
    The document contains graphs and tables showing the relationship between wavelength, absorbance, and concentration for the compounds Bromothymol Blue and Thymol Blue. Specifically, it shows absorbance measurements across wavelengths from 350-700 nm for the individual compounds and their sum, and it shows increasing absorbance at 618 nm with higher concentrations of Bromothymol Blue. The data and graphs indicate the absorbance properties and behaviors of these compounds at different wavelengths and concentrations.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 23309

    Wavelength vs.

    Absorbance
    1
    0.9
    0.8
    0.7
    0.6
    Absorbance (Bromothyl Blue)
    Absorbace

    Absorbance (Thymol Blue)

    0.5
    0.4
    0.3
    0.2
    0.1
    0
    350

    400

    450

    500

    550

    Wavelength (nm)

    600

    650

    700

    Wavelength vs. Absorbance (Sum of Bromothymol Blue and Thymol Blue)


    1.2

    0.8

    Absorbance

    Absorbance (Sum of Bromothymol Blue


    and Thymol Blue)

    0.6

    0.4

    0.2

    0
    350

    400

    450

    500

    550

    Wavelength (nm)

    600

    650

    700

    Wavelength vs. Absorbance (Bromothyl Blue)


    1
    0.9
    0.8
    0.7
    0.6

    Absorbance

    Absorbance (Bromothyl Blue)

    0.5
    0.4
    0.3
    0.2
    0.1
    0
    350

    400

    450

    500

    550

    Wavelengths (nm)

    600

    650

    700

    Wavelegth vs. Absorbance (Thymol Blue)


    1
    0.9
    0.8
    0.7
    0.6

    Absorbance

    Absorbance (Thymol Blue)

    0.5
    0.4
    0.3
    0.2
    0.1
    0
    350

    400

    450

    500

    550

    Wavelength (nm)

    600

    650

    700

    Absorbance of Bromothymol Blue at 618 nm vs. Concentration


    0.7

    f(x) = 0.0274249268x

    0.6

    0.5

    0.4

    Absorbance
    Linear (Absorbance)

    Absorbance
    0.3

    0.2

    0.1

    0
    0

    10

    15

    Concentration in M

    20

    25

    Absorbance vs. Wavelength


    1.1
    1
    0.9
    0.8
    0.7
    Absorbance (Bromothyl Blue)
    Absorbance (Thymol Blue)

    0.6
    Absorbance

    Absorbance (Sum of Bromothymol Blue


    and Thymol Blue)

    0.5
    0.4
    0.3
    0.2
    0.1
    0
    350

    400

    450

    500

    550

    Wavelength (nm)

    600

    650

    700

    Wavelengt Absorbance AbsorbanceAbsorbance (Sum of BConcentration


    Absorbance
    in mol/L of the Brothymol Blue
    370
    0.15
    0.551
    0.701
    23.2
    0.651
    380
    0.174
    0.606
    0.78
    18.6
    0.459
    390
    0.191
    0.665
    0.856
    13.9
    0.396
    400
    0.195
    0.731
    0.926
    9.3
    0.298
    410
    0.183
    0.799
    0.982
    4.64
    0.128
    420
    0.16
    0.845
    1.005
    430
    0.139
    0.872
    1.011
    434
    0.133
    0.874
    1.007
    440
    0.12
    0.87
    0.99
    450
    0.108
    0.821
    0.929
    460
    0.103
    0.75
    0.853
    470
    0.104
    0.657
    0.761
    480
    0.105
    0.571
    0.676
    490
    0.111
    0.471
    0.582
    500
    0.123
    0.374
    0.497
    510
    0.14
    0.299
    0.439
    520
    0.16
    0.245
    0.405
    530
    0.188
    0.2
    0.388
    540
    0.224
    0.172
    0.396
    550
    0.27
    0.159
    0.429
    560
    0.318
    0.159
    0.477
    570
    0.37
    0.167
    0.537
    580
    0.422
    0.179
    0.601
    590
    0.487
    0.196
    0.683
    600
    0.55
    0.195
    0.745
    610
    0.597
    0.171
    0.768
    618
    0.603
    0.143
    0.746
    620
    0.6
    0.131
    0.731
    630
    0.545
    0.092
    0.637
    640
    0.437
    0.056
    0.493
    650
    0.302
    0.031
    0.333
    660
    0.189
    0.016
    0.205
    670
    0.103
    0.008
    0.111

    8.604

    13.076

    Wavelengt Absorbance (Bromothyl Blue)


    370
    0.15
    380
    0.174
    390
    0.191
    400
    0.195
    410
    0.183
    420
    0.16
    430
    0.139
    434
    0.133
    440
    0.12
    450
    0.108
    460
    0.103
    470
    0.104
    480
    0.105
    490
    0.111
    500
    0.123
    510
    0.14
    520
    0.16
    530
    0.188
    540
    0.224
    550
    0.27
    560
    0.318
    570
    0.37
    580
    0.422
    590
    0.487
    600
    0.55
    610
    0.597
    618
    0.603
    620
    0.6
    630
    0.545
    640
    0.437
    650
    0.302
    660
    0.189
    670
    0.103

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