A Detailed Lesson Plan in Biology

I. Objectives: At the end of the 60 minute period at least 75% 0f the student should be able to
1. Explain the uses of Microscope
2. Label the part of Microscope
3. Manipulate the Microscope properly

II. Subject Matter: The Microscope
A Book: Reece Jane B, Campbell Biology. 9
th
.ed. Pearson Education, Inc., publishing as Pearson Benjamin
Cummings
B. Materials: Microscope, Specimens, Extension wire, Charts, Paper, tape, Scissors,
C. Value Integration: Self Reliance and Diligence

III. Procedure:
A. Learning Activities
Teachers Activity
A.Review
Good Morning Class

Let u pray first


Checking of Attendance, Say present and raise your hand if you are
here


Please be settle, pick-up the pieces of paper under your chair

So how was your morning today?

That good, Yesterday we have discussed the different types of cell,
please get and pass your assignment to the front

Today we will, learn about the microscope, its uses and part and
how to manipulate it

B. Motivation

I have a pictures of different parts of microscope, you need to
arrange the parts like what youre doing in a puzzle, do you
understand



C. Lesson proper

Microscope is a device used to view small things that cannot be
seen by naked.

Can a microscope be able to view the plant and animal cell?

There are different types of microscope, we have the simple,
compound and electric microscope

For our lesson today we will learn about the Compound
Students Activity

Good Morning Maam

(One assign student will lead the
Prayer)

(Students will say present and raise
their hands as he teacher call their
name)

(Students will pick up the pieces of
paper under their chair)

It was wonderful and Fun Maam

(Student will get their assignments
and pass it to the front)







Yes Maam
(student will do the activity)














Yes maam



(student listen attentively)

Microscope. Its parts and function and how to manipulate it

(shows a picture or chart of a microscope)



Condenser: This is a system of different lens elements which
is mounted beneath the stage of the microscope.
Base: This is the bottom part of the microscope, it contains
the lamp.
Coarse Focus: Also referred to as rough focus, this knob
raises and lowers the microscope stage quickly. It should
only be used in connection with the low magnification
lenses.
Eyepiece Lens: Also known as ocular lenses, they magnify
the image of the objective.
Fine Focus: This focus knob moves the stage up and down in
small steps. It is used to focus at different layers of the
specimens.
Head: This is the top part of the microscope. It carries the
eyepiece(s) and other optical elements.
Mechanical Stage: This type of stage is equipped with a
slide holder and two knobs to turn.
Nosepiece (or revolving nosepiece, turret): This part carries
the objectives. It can be rotated.
Objective Lens: This is a highly magnifying lens system; it is
located close to the specimen to be observed. The image of
the objective is then magnified again by the ocular lens
which is close to the eye.
Stage: This is the flat surface on which the slides are placed
on. It can be moved up and down for focusing.
Stage Clips: These are clips that hold the slide.
Trinocular Head: This microscope head has three exits, two
for viewing (for binocular vision) and a third exit to connect
a camera.

The proper way of using the microscope
(demonstration)
First familiarize yourself with the microscope.
Inspect the head. The head holds the optical elements,
which include the eyepiece and eyepiece tube, the nose
piece, and the objective lenses (there can be as many as 5
lenses).
Study the arm. The arm connects the head to the base. The
microscope is usually carried by the arm.
Examine the base. The base holds up the microscope and
provides the stage onto which samples are placed. The base
also contains the illuminator and the focusing knobs (both
fine and coarse).
Second, prepare your slide.
Position your sample between 2 pieces of glass to make a slide.


(student listen attentively)



















(student listen attentively while taking
down notes)


























(Student observe the demonstration
of the teacher and write down
important procedure)











Place the slide on the center of the stage over the glass hole.
Move the 2 stage clips over the sides of the slide to secure it
into place

Third, arrange the revolving nose piece and knobs.
Turn the nose piece turret until the shortest lens is above your
sample. It should click and feel rigid when it is in place. The
shortest objective lens is the least powerful and is the easiest
level to begin when magnifying an object.
Twist the coarse focus knob (the large one) on the side of the
base so the stage moves upward toward the short objective
lens. Perform this without looking into the eyepiece. It's
important to make sure the slide does not touch the lens. Stop
twisting the coarse knob just before the slide contacts the lens.

Fourth, focus the microscope.
Look through the eyepiece.
Arrange the illuminator until you've arrived at a comfortable
level of light. The brighter the illuminator shines, the better
you will be able to see your specimen, but do set it to the
maximum brightness if it hurts your eye.
Twist the coarse focus knob the opposite way as you had
before, so the stage moves away from the lens. Do this
slowly until the sample begins to focus.
Turn the fine focus knob the perfect the focus in greater
detail.

Fifth, magnify the image.
Reposition the slide as necessary to situate the area of
desire study directly under you gaze.
Switch lenses by rotating the nose piece turret to a longer
lens.
Perform necessary focusing adaptations.

Sixth, put the microscope away.
Turn off the power, lower the stage, remove your specimen and
cover the equipment with a dust-repellent cover.


Now, are you prepared to use the compound microscope?
Okay, Very Good

Now lets do the groupings because we only have ten pieces of
compound microscope that is available to our laboratory

Count from 1 -10, number one will stay here followed by number
Two and so on and so ford.

D. Application

Each group has one microscope the 5 prepared slides, your task are
to view the 5 specimen under the microscope and then draw what
you have seen.



E. Generalization

Can a microscope view the plant and animal cell?

So microscope is used to?

Correct, Microscope is an apparatus used in library to view an








































Yes Maam


(Student will count of from 1 to 10)


(Student will proceed to their
designated places)



(Student will do the activity; Student
will use the microscope by group on
their own, and view 5 types of
specimens. After viewing they will
draw what they have seen using the
microscope.)

Yes Maam

View small things that cannot seen
by naked eye


specimen, it has different type from simple to compound.

Microscope has different parts, that you need to familiarized to be
able to use the microscope properly

Do you have any question, if you do please raise your hand and ask
a question regarding to our topic






(Students raise their hand and ask
questions)

IV. Assessment
In a one whole piece of paper student will:
A. Explain the importance of microscope (10 POINTS)
B. Give or Label the parts that are ask in the picture below(14 POINTS)

C. Arrange the following sentence on how to use the microscope properly: Use 1to 6 number to show
your arrangement (6POINTS)
___a Turn off the power, lower the stage, remove your specimen and cover the equipment with a
dust-repellent cover.
___b Look through the eyepiece.
___c Turn the nose piece turret until the shortest lens is above your sample.
___ d Reposition the slide as necessary to situate the area of desire study directly under you gaze.
____e Inspect the head. The head holds the optical elements, which include the eyepiece and
eyepiece tube, the nose piece, and the objective lenses.
____ f Position your sample between 2 pieces of glass to make a slide.

Assessment Table:

Section Correct Responds
Number of Student
Percentage Decision
II-Star 45/50 90% Proceed
II-Armstrong 40/50 80% Proceed
II-Mendel 38/ 50 75% Proceed

V. Assignment
In a sheet of paper answer the numbers 1-10 on Test A and 1-5 on Test B, from your textbook page 12-14.

Answer
A-6
B-4
C-3
D-5
E-1