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TraceEvidencel:
HairsandFibers
111
Introduction
Hairisencounteredasphysicalevidenceina
widevarietyofcrimes.
Althoughitisnotyetpossibletoindividualizea
humanhairtoanysingleheadorbodythrough
itsmorphology,itstillhasvalueasphysical
evidence.
112
Introduction
Whenproperlycollectedandsubmittedtothe
laboratoryaccompaniedbyanadequate
numberofstandard/referencesamples,hair
canprovidestrongcorroborativeevidencefor
placinganindividualatacrimescene.
113
MorphologyofHair
Hairisanappendageoftheskinthatgrowsout
ofanorganknownasthehairfollicle.
114
Thelengthofahairextendsfromitsrootor
bulbembeddedinthefollicle,continuesintoa
shaft,andterminatesatatipend.
Itistheshaft,whichiscomposedofthreelayers
thecuticle,cortex,andmedullathatis
subjectedtothemostintenseexaminationby
theforensicscientist.
115
CuticleandCortex
Thecuticleisthescalestructurecoveringthe
exteriorofthehair.
Thescalesalwayspointtowardsthetipofthehair.
Thescalepatternisusefulinspeciesidentification.
116
CuticleandCortex
Thecortexisthemainbodyofthehairshaft.
Itsmajorforensicimportanceisthefactthat
itisembeddedwiththepigmentgranules
thatimparthairwithcolor.
Thecolor,shape,anddistributionofthese
granulesprovidethecriminalistwith
importantpointsofcomparisonamongthe
hairsofdifferentindividuals.
117
Medulla
Themedullaisacellularcolumnrunning
throughthecenterofthehair.
Themedullaryindexmeasuresthediameter
ofthemedullarelativetothediameterofthe
hairshaft.
Forhumans,themedullagenerallyoccupies
lessthanonethirdthediameteroftheshaft,
whileforanimalsitisgenerallyonehalfor
greater.
FORENSIC SCIENCE: An Introduction, 2nd ed.
By Richard Saferstein
118
Medulla
Themedullamaybecontinuous,
interrupted,fragmentedorabsent.
119
Medulla
Thepresenceofthemedullavaryfrom
individualtoindividualandevenamong
hairsofagivenindividual.
Medullaealsohavedifferentshapes,
dependingthespecies.
1110
Root
Therootandothersurroundingcellsinthe
hairfollicleprovidethetoolsnecessaryto
producehairandcontinueitsgrowth.
Whenpulledfromthehead,sometranslucent
tissuesurroundingthehairsshaftnearthe
rootmaybefound.Thisiscalledafollicular
tag.
ByusingDNAanalysisonthefolliculartag,the
hairmaybeindividualized.
FORENSIC SCIENCE: An Introduction, 2nd ed.
By Richard Saferstein
1111
ComparingStrands
Thecomparisonmicroscopeisanindispensable
toolforcomparingthemorphological
characteristicsofhair.
Whencomparingstrandsofhumanhair,the
criminalistisparticularlyinterestedin
matchingthecolor,length,anddiameter.
Acarefulmicroscopicexaminationofhairwill
revealmorphologicalfeaturesthatcan
distinguishhumanhairfromthehairof
animals.
FORENSIC SCIENCE: An Introduction, 2nd ed.
By Richard Saferstein
1112
ComparingStrands
Scalestructure,medullaryindex,and
medullaryshapeareparticularlyimportantin
animalhairidentification.
Otherimportantfeaturesforcomparing
humanhairare:
thepresenceorabsenceofamedulla.
thedistribution,shape,andcolorintensityof
thepigmentgranulespresentinthecortex.
1113
ComparingStrands
Themostcommonrequestistodetermine
whetherornothairrecoveredatthecrime
scenecomparestohairremovedfromthe
suspect.
However,microscopichairexaminationstend
tobesubjectiveandhighlydependantonthe
skillsandintegrityoftheanalyst.
1114
Questions
Canthebodyareafromwhichahair
originatedbedetermined?
Cantheracialoriginofhairbedetermined?
Cantheageandsexofanindividualbe
determinedfromahairsample?
Isitpossibletodetermineifahairwasforcibly
removedfromthebody?
FORENSIC SCIENCE: An Introduction, 2nd ed.
By Richard Saferstein
1115
Questions
Areeffortsbeingmadetoindividualizehuman
hair?
CanDNAindividualizeahumanhair?
1116
HairandDNA
RecentmajorbreakthroughsinDNAprofiling
haveextendedthistechnologytothe
individualizationofhumanhair.
TheprobabilityofdetectingDNAinhairroots
ismorelikelyforhairbeingexaminedinits
anagenorearlygrowthphaseasopposedtoits
catagen(middle)ortelogen(final)phases.
1117
HairandDNA
Often,whenhairisforciblyremoveda
folliculartag,atranslucentpieceoftissue
surroundingthehairsshaftneartherootmay
bepresent.
Thishasproventobearichsourceofnuclear
DNAassociatedwithhair.
1118
HairandMitochondrialDNA
MitochondrialDNAcanbeextractedfromthe
hairshaft.
MitochondrialDNAisfoundincellular
materiallocatedoutsideofthenucleusanditis
transmittedonlyfromthemothertochild.
Asarule,allpositivemicroscopichair
comparisonsmustbeconfirmedbyDNA
analysis.
FORENSIC SCIENCE: An Introduction, 2nd ed.
By Richard Saferstein
1119
CollectionandPreservation
Asageneralrule,forensichaircomparisons
involveeitherheadhairorpubichair.
Thecollectionof50fulllengthhairsfromall
areasofthescalpwillnormallyensurea
representativesamplingofheadhair.
Aminimumcollectionoftwodozenfulllength
pubichairsshouldcovertherangeof
characteristicspresentinpubichair.
FORENSIC SCIENCE: An Introduction, 2nd ed.
By Richard Saferstein
1120
CollectionandPreservation
Hairsamplesarealsocollectedfromthevictim
ofsuspiciousdeathsduringanautopsy.
1121
TypesofFibers
Naturalfibersarederivedinwholefromanimal
orplantsources.
Examples:wool,mohair,cashmere,furs,andcotton.
Manmadefibersaremanufactured.
Regeneratedfibersaremanufacturedfromnatural
rawmaterialsandincluderayon,acetate,and
triacetate.
Syntheticfibersareproducedsolelyfromsynthetic
chemicalsandincludenylons,polyesters,and
acrylics.
1122
TypesofFibers
Polymers,ormacromolecules,aresynthetic
fiberscomposedofalargenumberofatoms
arrangedinrepeatingunitsknownas
monomers.
1123
FiberEvidence
Thequalityofthefiberevidencedependson
theabilityofthecriminalisttoidentifythe
originofthefiberoratleastbeabletonarrow
thepossibilitiestoalimitednumberofsources.
Obviously,iftheexaminerispresentedwith
fabricsthatcanbeexactlyfittedtogetherat
theirtornedges,itisavirtualcertaintythatthe
fabricswereofcommonorigin.
1124
FiberEvidence
Microscopiccomparisonsbetweenquestioned
andstandard/referencefibersareinitially
undertakenforcoloranddiameter
characteristics,usingacomparisonmicroscope.
Othermorphologicalfeaturesthatcouldbe
importantincomparingfibersare:
Lengthwisestriationsonthesurfaceofthefiber.
Thepresenceofdelusteringparticlesthatreduce
shine.
Thecrosssectionalshapeofthefiber.
FORENSIC SCIENCE: An Introduction, 2nd ed.
By Richard Saferstein
1125
FiberEvidence
Compositionaldifferencesmayexistinthedyes
thatwereappliedtothefibersduringthe
manufacturingprocess.
1126
MethodsforFiberComparison
Thevisiblelightmicrospectrophotometerisa
convenientwayforanalyststocomparethe
colorsoffibersthroughspectralpatterns.
Amoredetailedanalysisofthefibersdye
compositioncanbeobtainedthrougha
chromatographicseparation.
1127
MethodsforFiberComparison
Infraredspectrophotometryisarapidand
reliablemethodforidentifyingthegenericclass
offibers,asdoesthepolarizingmicroscope.
Dependingontheclassoffiber,eachpolarized
planeoflightwillhaveacharacteristicindexof
refraction.
1128
CollectionandPreservation
Theinvestigatorstaskoflookingforminute
strandsoffibersoftenbecomesoneofidentifying
andpreservingpotentialcarriersoffiber
evidence.
Relevantarticlesofclothingshouldbepackaged
carefullyinseparatepaperbags.
Ifitisnecessarytoremoveafiberfromanobject,
theinvestigatormustusecleanforceps,placeitina
smallsheetofpaper,foldandlabelthepaper,and
placethepaperpacketinsideanothercontainer.
FORENSIC SCIENCE: An Introduction, 2nd ed.
By Richard Saferstein
1129