GEN NHX2

>gi|150247014|emb|AM748092.1| Mesembryanthemum crystallinum mRNA for Na+/H+ antiporter (nhx2 gene)

ATGGAGGATCAGCTGATTTCTCCGGCCGACTCGCGTGGGAGTCCGGCGAAGGAGCAGCAAGCAGCCGGTGTCGGAATCCTTCTTCAGATTATG
ATGCTTGTTCTCTCTTTCATTCTCGGTCATGTCTTGCGTCGTCACAAATTCTTTTACATTCCTGAAGTTAGTGCCTCTCTCTTGATTGGTTTAGTTGT
CGGTGGACTTGCTAACATCTCAAATACAGAAACCAGCATCAGGGGATGGTTTAACTTCCACGAGGAGTTTTTCTTCCTTTTCTTGTTACCTCCAAT
CATATTTCAATCAGGGTTCATTCTTTCACCTAAACCATTCTTCTCAAACTTTGGAGCTATTGTGACATTTTCTATTCTTGGGACCTTCATAGCTTCAG
TGTTAACTGGCCTTCTTGTGTACCTTGGGGGAATCACCTTTCTTATGTACAAACTTCCGTTGATTGAATGCATGATGTTTGGTGCTCTCATATCATC
AACTGATCCTGTCACAGTTTTAGCAATATTCCAGGAACTTGGCACTGATGTGAATCTTTACGCCTTGGTGTTTGGAGAATCTGTTTTGAATGATGCT
GTTGCAATTTCCTTGTACAGGACAATGTCGTTGTTGAACAGTCATATGTCAACTGGACAGAATATCTTCACTCTCGTTGTTGGGTTTCTTGAGACTT
TTGTTGGCTCATTGTCTTCAGGGGTTGGCGTTGGATTTATACCTGCTCTCCTTTTCAAGTATGCAGGTCTAGATATTGATAACCTCCAGAACTTGGA
AAGCTGTCTTTTCGTTCTTTTTCCATATTTCTCGTACATGCTTGCGGAAGGTCTTGGTCTCTCTGGCATTGTATCAATATTGTTTACCGGGATTGTCA
TGAAGCACTATACTTTCTCAAATTTGTCAGAGAAGTCTCAACAATTTGTATCTGAATTTTTCCATCTAATATCATCGTTAGCGGAGACTTTTATATTTAT
ATACATGGGCCTCGATATTGTCATGGAAAAGCACAGCTGGTCACATGTCGGCTTTATATTCTTCTCGATTATATTTATTGTGATTTCAAGGGCTGTCA
ATGTCTTCTCTTGTGGATATCTGATAAATCTGGCTCGTCCTGCACATCGAAAGATACCAATGAAACATCAAAAAGCACCTTGGTACAGCGGGCTAA
GAGGGGCTATGGCTTTTGCTCTTGCTCTCCAATCTGTTCATGATCTACCTGAAGGACATAGCCAAACAATTTTCACAGCGACCACAGCAATAGTTG
TTTTGACGGTTTTACTTATTGGAGGATCAACTGGCACCATGCTTGAAGCCTTGCAAGTTATAGGTGATAGTAATGAAGGCCACATAGGGGATGGCT
TCAACAGGAGTGATGGCTACGTATCTCCTTCCCAAGATGAAGAAGAGTCATCTTCAAATAGATTCAAGATGAGACTAAAAGAGTTCCACCGAAGTG
CACCTTCTTTCTCATCATTGGACAAAAACTACCTGACTCCGTTCTTCACTACCCAGTCCGGGGATGAAGAAGAACATGAAACTCTGATGCCCAGT
CCAAGAAGAGAAGTCTATCTCAGTCGCAACTGA

Nombre

Secuencia

Inic
io
1

Termi
na
20

McNHX2.15
81F
McNHX2GF

ATGGAGGATCAGCTGAT
TTC
GATTTCTCCGGCCGACTC

15

32

McNHX2PCR
F
McNHX2
int3F

GGCTATGGCTTTTGCTCT
TG
CAGGACAATGTCGTTGTT
GA

117
0
597

1189

ATTGGAGGATCAACTGG
CAC
TTCTTCACTACCCAGTCC
GG
McNHX2.15
AGTCTATCTCAGTCGCAA
81R
CTGA
McNHX2 intR GCTCATTGTCTTCAGGG
GTT
McNHX2
GATTTCAAGGGCTGTCA
int3R
ATG

127
9
149
4
155
9
683

1298

105
3

1072

McNHX2PCR
R
McNHX2GR

616

1514
1581
702

Senti
do
Forwar
d
Forwar
d
Forwar
d
Forwar
d

Tm
60.7

Dise

Carlos

64.8

Julio

63.6

Norber
to
Julio

62.2
5

Revers 63.9 Norber

e
to
Revers 64.0
Julio
e
Revers 60.7 Carlos
e
Revers 62.99 Julio
e
Revers 62.02 Julio
e

Primers a pedir
Nombre
McNHX2
intR
McNHX2
int3F
McNHX2
int3R

53
AACCCCTGAAGACAATGAGC
CAGGACAATGTCGTTGTTGA
CATTGACAGCCCTTGAAATC

http://biotools.umassmed.edu/cgi-bin/primer3plus/primer3plus.cgi