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pH on
urease
Introduction
Prediction (Pro 3)
The urease enzyme reacts with urea,
the best pH is 7.
Procedure 1
Results
Name of
tubes
Color of
Reddish
cabbage
indicator
Dropping
(2 drops )
Color after
dropping
Tube A
purple
Citric acid
pink
Tube B
purple
NaCO3
blue
Tube C
purple
Distilled water
purple
Results
Questions
Procedure 2:
Soya
suspension
2ml.
distilled
water
2ml.
Urea 2ml.
Results
Results
Name of Color of
Adding
tubes
indicator substrates
(each substrate
= 2 ml.)
Color
when fills
substrate
s
Color after
adding 5
minute
Tube A
purple
Soya suspension
and 10% urea
blue
green blue
Tube B
purple
10% urea
purple
purple
Tube C
purple
Soya suspension
and distilled water
Light
purple
Light purple
Questions
Procedure 3:
4
drops
2
drops
Drops 3
baking
soda
Drop
acetic
acids
8
drops
6
drops
Results
Name of
tube
Adding
citric
acid
Adding
baking
soda
Color after
vortex
pH by pH
paper
strips
3 ml
Blue
2 drops
3 ml
Purple
4 drops
3 ml
Purple pink
8 drops
3 ml
Pink
16 drops
3 ml
Light pink
Results of pH by using
pH paper strips
Name of tube
pH
4
drops
2
drops
Drops 3
baking
soda
Drop
acetic
acids
8
drops
6
drops
Test pH by Vernier
Name of
tube
color
Green
blue
Blue
Purple
Pink
Red Pink
pH
10.23
7.58
6.44
4.38
4.42
Question
How does pH affect the urease activity? How do
you know?
Ans. pH 7 is the best state for urease to react with urea.
We saw in the test tubes that the tube which had pH
around 7, the color is changing faster than other, when we
left it about 3 min.
Question
Because basic pH will also change the color of the
indicator to blue/green which is very similar when
ammonia has been produced by the urease
enzyme, how can we make sure that the color
change is actually due to the urease activity? Is it
possible to test this?
Ans. Yes, prove by doing an experiment by put the
urea reacts with the ureas first. Then fill the indicator
to see the change.
Conclusion
On this experiment, we have learned about the effect of pH to
enzyme. In this experiment we figure out what is the best range of
pH of the urease to create reaction. The best range of pH to affects
to reaction is around 7 same as our prediction. We can see from the
result that the first test tube which had pH around 7, the color is
changing more and faster from others while leaving for 3 min. We
also ad errors because we dont know how to use the probe to
measure the pH properly. There were 2 tubes that we fill the acid in
different amount, the one that had more acid should had less pH,
but the probe is show up that tube was acidic more than the
another one. Next time we will fix it.
Member
Arisara K. Now
Noramon K. Prim
Piyaporn R. Premier
Nuttacha I. Fah
Inchanok P. Inn