(Quaternary structure)

Myoglobin

Hemoglobin
Mathews et al (2000) Biochemistry (3e) p.214

(Hb) (Mb) Hb
Mb Hb
Mb
Mb 22

P4-1

(R)
relaxed state

Lung

Hb

Hb

Muscle

(T)

Hemoglobin tense state

Myoglobin
Juang RH (2007) BCbasics

Hb Mb Hb
Hb

Hb
heme Hb

Hb
Hb
Mb Hb Hb

Hb
tense (T)
relaxed (R) T R

Mb

P4-2

Hemoglobin

80
60
40
20
0

20

40

60

80

100

Partial pressure of oxygen (pO2, mmHg)

120

Adapted from Garrett & Grisham (1999) Biochemistry (2e) p.480

Percent O2 saturation

100

Myoglobin

Hb S
Mb

Hb Hb

Mb

Hb

P4-3

 heme

Stryer (1995) Biochemistry (4e) p.151

hemeheme
heme
Hb His imidazole E7 His

X-ray

Lehninger Principles of Biochemistry (4e) p. 136

P4-4

Garrett & Grisham (1999) Biochemistry (2e) p.486

Stryer (1995) Biochemistry (4e) p.163

E7 His heme F8 His

E7 His F8 His
F8 helix Hb (T
form R form)
Hb

Hb Hb
Hb Mb Hb Mb

Heme
heme heme
Lehninger Principles of Biochemistry (4e) p. 855 heme

P4-5

WIKIPEDIA

Cartoon by I. Geis

I. Geis Hb
Geis

Wikipedia hemoglobin

P4-6

1
Juang RH (2007) BCbasics

P4-7

4
Mathews et al (2000) Biochemistry (3e) p.195

( Mb) ( Hb)

P4-8

()

( helix, sheet)
Domain ( )
(monomer)

(dimer)

Juang RH (2007) BCbasics

helix sheet

P4-9

GluVal

EF pocket
Mathews et al (2000) Biochemistry (3e) p.236

Hb
heme

(
)

Pauling
molecular disease

P4-10

Mathews et al (2000) Biochemistry (3e) p.239

Glu Val

Glu Val

P4-11

Conformation

Juang RH (2007) BCbasics

()

( ... )

P4-12

hemoglobin

pyruvate dehydrogenase

actin, myosin, virus

Juang RH (2007) BCbasics

(1) (2)
Pyruvate dehydrogenase

Lehninger Principles of Biochemistry (4e) p. 604 pyruvate dehydrogenase p. 182 actin

myosin

P4-13

product

substrate

Garrett & Grisham (1999) Biochemistry (2e) p.573

Lehninger Principles of Biochemistry (4e) p. 697

P4-14

Garrett & Grisham (1999) Biochemistry (2e) p.173

Helix coiled coil

()
Lehninger Principles of Biochemistry (4e) p. 126 keratin

P4-15

Alberts et al (2002) Molecular Biology of the Cell (4e) p.153

Lehninger Principles of Biochemistry (4e) p. 145

P4-16