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TNF aVCAM 1mRNA

20101229 16:
47:
07Wednesday

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.

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Rest
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[
e]

30

[
]
[
]

(
TNF )
(
ECV)

1(
VCAM 1)
mRNA

(
12.
52550g/
ml
)
TNF ;

.
.
.

TNF 25g/
ml

816244825g/
ml
TNF

16(
2.
5%5%10%)

(
RT PCR)

VCAM 1mRNA

TNF VCAM 1mRNA

(
P<0.
01)
(
P>0.
05)
25g/
ml

TNF 162448

VCAM 1mRNA(
P<0.
05P<0.
01)

VCAM 1mRNATNF

VCAM 1mRNA(
P<0.
01)

(
P>0.
05)

1TNF
Abs
t
r
ac
tObj
ec
t
i
v
e:
Toi
nves
t
i
gat
et
heef
f
ectofNi
aoSuanLi
Xi
ancont
ai
ni
ngser
um onex
pr
es
si
onofv
ascul
ar
c
el
l
adhesi
onmol
ec
ul
e(
VCAM 1)mRNAi
nvascul
arendot
hel
i
al
c
el
l
si
nducedbyTNF .
Met
hods:
Vascul
ar
endot
hel
i
alcel
l
swasc
ul
t
ur
edandst
i
mul
at
edwi
t
hdi
f
f
er
entdensi
t
y
(
12.
5,
25,
and50g/
ml
)ofTNF i
nv
i
t
r
o,
.
At
t
hes
amet
i
me,
t
hecel
l
swer
eco i
ncubat
edwi
t
h25g/
mldensi
t
yofTNF f
or8,
16,
24,
and48hour
s
.
They

wer
ei
nt
er
v
edbydi
f
f
er
entdensi
t
y(
2.
5%,
5%,
and10%)
ofNi
aoSuanLi
Xi
anc
ont
ai
ni
ngser
um.Theex
pr
ess
i
onof
VCAM 1mRNAi
nv
asc
ul
arendot
hel
i
alcel
l
swasdet
ect
edbyRT PCR.
Res
ul
t
s:
Theex
pr
es
si
onofVCAM 1

mRNAi
nv
asc
ul
arendot
hel
i
alcel
l
si
nducedbyTNF i
ncr
eas
edsi
gni
f
i
cant
l
y
(
P<0.
01)
,
butnodi
f
f
er
enc
ewas

not
edbet
weendi
f
f
er
entdens
i
t
ygr
oups.
Theex
pr
essi
onofVCAM 1mRNAi
nv
as
cul
arendot
hel
i
al
cel
l
si
nduced

byt
he25g/
mldens
i
t
yofTNF i
ncr
easedsi
gni
f
i
cant
l
yaf
t
er16,
24,
and48hour

i
nc
ubat
i
on(
P<0.
01)
.
Ni
aoSuanLi
Xi
ancont
ai
ni
ngser
um atal
lex
per
i
ment
aldens
i
t
ysi
gni
f
i
cant
l
ydown r
egul
at
ed
t
heex
pr
es
si
onofVCAM 1mRNA(
P<0.
05P<0.
01)
.
Concl
usi
on:
Down r
egul
at
i
ngt
heex
pr
ess
i
onofVCAM 1

i
nvas
c
ul
arendot
hel
i
alc
el
l
smaybeoneoft
hemechani
smsofNi
aoSuanLi
Xi
anr
educ
i
ngt
hei
nf
l
ammat
or
y

i
nj
ur
es
.

2012

Keywor
dsher
bal
Ni
aoSuanLi
Xi
anvascul
arcel
ladhesi
onmol
ec
ul
eTNF vas
cul
arendot
hel
i
alc
el
l
s

(
c
or
oar
yhear
tdi
s
eas
e,
CHD)

(
at
her
osc
l
er
osi
s
,
As)

(
)

1
1.
1

journal.9m ed.net/htm l/qikan/zxyjh/zjzxyjhzz/2010122012/lz/20101230091311303_554728.htm l

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RPMI
1640 (
FCS)
Gi
bc
oBr
l
;
B
Si
gma;
RNATr
i
z
ol
PCR
1.
2
(
endot
hol
i
alcel
lofvessel
s,
ECV)

1.
3

10%RPMI1640(
100u/
ml
)
0.
25%

0.
02%EDTA5%CO237

1.
4
1.
4.
1

415ml
60ml
40

1.
4.
2
42522
15ml
60ml
40
2
2.
1TNF
VCAM 1mRNA 4(
10%RPMI1640
)
TNF (
TNF 12.
525
50g/
ml
)

2.
2TNF
VCAM 1mRNA 5(
10%RPMI1640
)
8162448TNF (
TNF 25g/
ml
81624
48)

2.
3TNF
VCAM 1mRNA 5(
10%RPMI1640
)
TNF (
TNF 25g/
ml
)

(
TNF 2.
5%5%10%
10%)
16
2.
4RT PCRVCAM
1mRNA 6070%24
PBS12Tr
i
z
ol
RNA
(
RT PCR)
GAPDHPCR955mi
n;
9530s
5630s
7230s
35
;
7210mi
n;
1.
7%Bi
oRadquani
t
yone
VCAM 1mRNAGAPDH
VCAM 1700bp5 AGTGGTGGCCTCGTG AATGG 3
5CTG TGTCTCCTG TCTCCG
CT 3
;
act
i
n540bp5 GTG GGG CGCCCCAGG CACCA 3
5 CTCCTTAATGTCACG CAC
GATTTC 3

2.
5
(
s
)
SPSS11.
5FP<0.
05

3
3.
1TNF
VCAM 1mRNA TNF VCAM 1mRNA
(
P<0.
01)
111TNFVCAM1mRNA
3.
2TNF
VCAM 1mRNA 25g/
ml
TNF VCAM 1mRNA
162448(
P<0.
01)
(
P>0.
05)
22
3.
3TNF
journal.9m ed.net/htm l/qikan/zxyjh/zjzxyjhzz/2010122012/lz/20101230091311303_554728.htm l

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 VCAM 1mRNA TNF

VCAM 1mRNA(
P<0.
05P<0.
01)
332TNF
VCAM 1mRNA2MMARK;
A;
B8TNF ;
C16TNF
;
D24TNF ;
E48TNF 3TNF
VCAM 1mRNA
4
(
monocyt
e,
MC)
(
endot
hel
i
al
c
el
l
,
EC)
(
macr
ophage,
MP)
;
MP(
s
moot
hmusc
l
e
c
el
l
,
SMC)
AS[
1]

3[
2]

1(
VCAM 1)
I
NCAMCD106I
L 1I
L 4TNFECVCAM 1
101472TNK(
VLA 4)
EC
VCAM 1[
3]
VCAM 1
[
4]
VCAM 1C(
PKC)

;
PKC[
4]

TNF
VCAM 1mRNATNF (

12.
52550g/
ml
)
VCAM 1mRNA(
P<0.
01)
TNF
VCAM 1mRNA162448
(
2.
5%5%10%)
TNF VCAM 1mRNA(
P<0.
05P<0.
01)

TNF VCAM 1mRNA

I
CAM 1TNF (
)

TNF AS

\
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1\
]Munr
oJ
M,
Cot
r
anRS.
Thepat
hogenesi
sofat
her
oscl
er
osi
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at
her
ogenesi
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r
oniM,
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.
Mi
gr
at
i
onofnat
ur
al
k
i
l
l
ercel
l
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ossendot
hel
i
al
cel
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ayer
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I
mmunol
,
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[
4\
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ol
l
esAR,
Qi
nX,
Spr
i
ngerTA.
Char
act
er
i
z
at
i
onoft
hef
unc
t
i
onofi
nt
er
cel
l
ul
aradhesi
onmol
ecul
e
(
I
CAM) 3andcompar
i
s
onwi
t
hVCAM 1andI
CAM 2i
ni
mmuner
esponses
\
[
J
\
]
.
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p
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1994,
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:
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Emai
l

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