HIV Testing Algorithm (excludes HIV rapid testing)

HIVE/9333 HIV-1/-2 Antibody Evaluation, Serum If indicated, all other tests listed are automatically performed and charged separately If suspect acute HIV infection or highrisk patient, see* *Suspect acute HIV infection or high-risk patient

HIV-1/2 antibody screen

Negative

If HIV antibody screen already performed order 1 of the following: WBAR/23878 HIV-1/-2 Antibody Confirmatory Evaluation, Serum OR WBANF/32466 HIV-1/-2 Antibody Confirmatory Without Immunofluorescence Assay, Serum

Reactive STOP Repeat HIVE/9333 in 3 months for non-high-risk patients, if clinically indicated

HIVQU/81958 HIV-1 RNA Quantification, Plasma OR PHIV/88635 HIV-1 Proviral DNA Qualitative Detection by PCR, Blood

HIV Types 1 and 2 Confirmatory Evaluation, Serum

Negative (high-risk patient)

Negative (low-risk patient)

Positive

HIV-1 antibody confirmation by Western blot

If suspect HIV-2 FHV2Q/91490 HIV-2 DNA/RNA Qualitative Real-Time PCR

If suspect HIV-1

STOP

HIV-1 infection

Indeterminate or Unreadable

If suspect acute HIV infection or high-risk patient, see*

Negative

Positive

HIV-1 infection

Repeat serologic testing HIVE/9333 HIV-1/-2 Antibody Evaluation, Serum in 2 to 4 weeks

HIV-1 Antibody Confirmation by Immunofluorescence, Serum

HIV-2 Antibody Screen, Serum

Positive

Negative
(high-risk patient)

Positive

Negative

Negative

Positive

HIV-2 infection

HIV-1 infection

HIV 2 Antibody IBL (immunoblot) If suspect acute HIV infection or highrisk patient, see* If suspect acute HIV-2 infection

Repeat serologic testing HIVE/9333 in 2 to 4 weeks

For non-high-risk patients, repeat serologic HIV testing HIVE/9333 alone, in 2 to 4 weeks

Positive

Negative

HIV-2 infection

STOP Repeat HIV-2 antibody screen HIV2/86702 HIV Type 2 Antibody Screen, Serum (symptomatic patient) or HIV2M/60356 HIV-2 Antibody Screen, Serum (nonsymptomatic individuals) in 3 months for non-highrisk patients, if clinically indicated

Order Options Unit Code Tests Performed

HIV-1-2 Antibody Screen HIV Types 1 and 2 Confirmatory Evaluation, Serum HIV-1 Antibody Confirmation by Western blot HIV Type 1 Antibody Confirmation by Immunofluorescence, Serum HIV Type 2 Antibody Screen, Serum HIV 2 Antibody IBL (Immunoblood)

HIVE/ 9333
X X X X X X

WBAR/ 23878
X X X X X

WBANF/ 32466
X X X X 12/11

Mayo Foundation for Medical Education and Research (MFMER). All rights reserved. MAYO, Mayo Medical Laboratories and the triple-shield Mayo logo are trademarks and/or service marks of MFMER.

Reviewed 07/2012