SANTA CRUZ BIOTECHNOLOGY, INC.

Actin (C-2): sc-8432

The Power to Question

BACKGROUND
All eukaryotic cells express actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion, Rac regulates actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

STORAGE
Store at 4 C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.

DATA
132 K 90 K 55 K 43 K 34 K -

< Actin

REFERENCES
1. Doolittle, R.F. 1995. The origins and evolution of eukaryotic proteins. Phil. Trans. Royal Soc. London Biol. Sci. 349: 235-240. 2. Maccioni, R.B., et al. 1995. Role of microtubule-associated proteins in the control of microtubule assembly. Physiol. Rev. 75: 835-864.

Actin (C-2): sc-8432. Western blot analysis of Actin expression in C32 whole cell lysate.

Actin (C-2) PE: sc-8432 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2866..

SOURCE
Actin (C-2) is a mouse monoclonal antibody epitope mapping at the C-terminus of human origin.

PRODUCT
Each vial contains 200 g IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-8432 P, (100 g peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). Available as PE conjugate for flow cytometry, sc-8432 PE, 100 tests. Available as agarose conjugate for immunoprecipitation, sc-8432 AC, 500 g/0.25 ml agarose in 1 ml. Available as HRP conjugate for Western blotting, sc-8432 HRP, 200 g/1 ml. Available as fluorescein (sc-8432 FITC) or rhodamine (sc-8432 TRITC) conjugates for immunofluorescence, 200 g/1 ml.

Actin (C-2): sc-8432. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.

Actin (C-2): sc-8432. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue showing cytoskeletal localization.

SELECT PRODUCT CITATIONS

1. Xiao, G., et al. 2001. Retroviral oncoprotein Tax induces processing of NF-kappaB2/p100 in T cells: evidence for the involvement of IKKalpha. EMBO J. 20: 6805-6815. 2. Perskvist, N., et al. 2002. Mycobacterium tuberculosis promotes apoptosis in human neutrophils by activating caspase-3 and altering expression of Bax/Bcl-xL via an oxygen-dependent pathway. J. Immunol. 168: 6358-6365. 3. Castro-Alcaraz, S., et al. 2002. NF-kappa B regulation in human neutrophils by nuclear I kappa B alpha: correlation to apoptosis. J. Immunol. 169: 3947-3953. 4. Crone, S.A., et al. 2003. Colonic epithelial expression of ErbB2 is required for postnatal maintenance of the enteric nervous system. Neuron 37: 29-40. 5. Romanelli , RG., et al. 2004. Expression and function of gonadotropinreleasing hormone (GnRH) receptor in human olfactory GnRH-secreting neurons: an autocrine GnRH loop underlies neuronal migration. J. Biol. Chem. 279: 117-126.

APPLICATIONS
Actin (C-2) is recommended for detection of a broad range of Actin isoforms of mouse, rat, human and Xenopus origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [12 g per 100500 g of total protein (1 ml of cell lysate)], immunofluorescence and immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry (1 g per 1 x 106 cells). Suitable for use as control antibody for Actin siRNA (h): sc-29191 and Actin siRNA (m): sc-29192. Positive Controls: C32 whole cell lysate: sc-2205, BC3H1 cell lysate: sc-2299 or Sol8 cell lysate: sc-2249.

RESEARCH USE
For research use only, not for use in diagnostic procedures.

Santa Cruz Biotechnology, Inc.

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