Professional Documents
Culture Documents
I. HPLC Columns
Caution : Methanol and acetonitrile are hazardous substances, do not use for medical purpose. Always process in a laboratory hood and wear an eye protection and a mask.
(Reference) Influence of organic solvent composition in mobile phase on the retention time.
1
2 5 10
70:30
7.89min 0.55min
V. Applications
69:31
10
8.44min 0.66min
68:32
10
9.10min 0.61min
67:33
10
9.71min 0.73min
66:34
10
Column Flow rate Detection Temperature Sample
10.44min
Special attention should be paid to measure correct amount of organic solvent as retention time is significantly changed by 1% different composition.
5C18-MS-II 4.6 mmI.D. x 150 mm 1.0ml/min 254nm 0.16AUFS 30oC 1. Uracil 2. Naphthalene
VII. Index
187
Technical Information
I. HPLC Columns
V. Applications
VII. Index
188