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Code No: 07A3BS06

Set No. 1

II B.Tech I Semester Regular Examinations, November 2008 APPLIED CHEMISTRY AND BIOCHEMISTRY (Bio-Medical Engineering) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. (a) Dene the terms: i. Specic conductance ii. Equivalent conductance iii. Molar conductance.

(b) Explain why specic conductance decreases and equivalent conductance increases with dilution? (c) How do you determine the cell constant with dilution? 2. (a) What are elastomers? Give examples. (b) Give the monomers of the following: i. Teon ii. Bakelite.

(c) Give an account of vulcanization of rubber and compare natural rubber with Vulcanized rubber? [4+4+8] 3. (a) Discuss the importance of:

i. Pour point ii. Flash point iii. Oxidation Stability in the selection of a good lubricant? (b) Dene viscosity index and saponication value and their signicance. (c) Write a brief note on hydrodynamic lubrication? 4. (a) Illustrate how particles are separated by dierential centrifugation? (b) What is the dierence between nuclear envelop and cell membrane in terms of structure and function? [8+8] 5. (a) Explain how enzymes are useful for detecting human immuno virus (HIV)? (b) Explain holoenzyme composition. 6. (a) Give an account of the cholesterol biosynthesis. (b) What is plasma cholesterol estimation and what is its importance. 7. (a) Describe the origin of platelets and explain their function. (b) Describe a method for the estimation of glucose present in a urine sample. [8+8] 1 of 2 [10+6] [10+6] [6+4+6]

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[ 6+4+6]

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Code No: 07A3BS06

Set No. 1

8. (a) Write the general methods of biochemical analysis of glucose present in the blood. (b) Name the dierent types of chromatographic techniques and explain the use of UV lamp for spotting in TLC. [10+6]

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Code No: 07A3BS06

Set No. 2

II B.Tech I Semester Regular Examinations, November 2008 APPLIED CHEMISTRY AND BIOCHEMISTRY (Bio-Medical Engineering) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. (a) How do you determine the pH of a solution using a reference electrode? (b) What is single electrode potential? How do you determine the emf of a galvanic cell? [8+8] 2. (a) What is polymerization?

(b) Describe the process of co-polymerization with an example. (c) Dierentiate thermoplastics from thermosetting plastics?

3. (a) What are the various additives used to induce or improve the essential properties of lubricating oils. (b) Distinguish between uid lm and boundary lubrication. (c) Dene viscosity and viscosity index? What is its signicance? 4. (a) What are cell organelle?

(b) Describe each organelle and its function. (c) What are the dierences between plant and animal eukaryotic cells? [2+8+6] 5. (a) Dene specicity of an enzyme and explain dierent types of specicities exhibited by enzymes. (b) Dierentiate between the following terms:i. Competitive and non-competitive inhibition ii. Coenzyme and isoenzymes. [8+8]

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[4+8+4]

[6+4+6]

6. (a) Write a note on the structure of steroids. (b) Explain the functions of cholesterol. 7. (a) What are erythrocytes? Explain their origin, structure and functions. (b) Describe the structure and functions of a hemoglobin. 8. (a) Explain various detectors and burners involved in ame photometry. (b) What is the signicance of excess presence of glucose in blood? [10+6] [8+8] [8+8]

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Code No: 07A3BS06

Set No. 3

II B.Tech I Semester Regular Examinations, November 2008 APPLIED CHEMISTRY AND BIOCHEMISTRY (Bio-Medical Engineering) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. (a) Outline the experimental setup and procedure to determine the specic conductivity of 0.1N Solution of KCl at room temperature. (b) What is cell constant? What is its unit? How do you determine it? 2. (a) Give the monomers of the following: i. PVC ii. HDPE iii. Polythene [8+8]

(b) Describe the preparation and engineering uses of Bakelite?

3. Suggest suitable lubricants for each of the following systems with justication: (a) Internal Combustion Engines (b) Cutting tools (c) Gears (d) Steam turbines.

4. Write short notes on:

(a) Electron transport complexes (b) Respiratory inhibitors (c) Golgi complex or golgi apparatus (d) Cytochromes. [16]

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[8+8]

[16]

5. (a) Describe the reversible and irreversible mode of inhibition of enzymes with suitable examples. (b) Explain the laws of the Spectrophotometry and how these are useful in the study of enzymes. [6+10] 6. (a) Describe the replication of DNA. (b) Write short notes on: i. Replication fork ii. RNA polymerase iii. Reverse Transcription.

[7+9]

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Code No: 07A3BS06

Set No. 3

7. (a) What is electrophoresis? How it is useful? Write a note on zone electrophoresis. (b) What is meant by salting out of proteins? Explain the separation of blood serum proteins by electrophoresis. [8+8] 8. (a) What is normal and reversed phase partition chromatography? (b) Write briey on the separation of amino acids by one and two dimensional chromatography. [8+8]

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Code No: 07A3BS06

Set No. 4

II B.Tech I Semester Regular Examinations, November 2008 APPLIED CHEMISTRY AND BIOCHEMISTRY (Bio-Medical Engineering) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. (a) Write down the Nernsts equation and explain the various terms involved? (b) What are its important applications? (c) Explain the source of current in a galvanic cell with an example and the necessary equations. [6+4+6] 2. (a) How is rubber isolated from latex? (c) Write a note on vulcanization of rubber? (b) Describe the preparation and engineering uses of Bakelite?

3. (a) Describe hot-limesoda process of water treatment and what are its advantages over cold process? (b) Calculate lime and soda required for treating 10,000 litres of a sample of water containibg the following in milligrams per litre.Calcium Carbonate 8.1, Magnesium Sulphate 12, calcium sulphate: 13.6,Sodium Chloride 4.6. [10+6] 4. (a) What is Golgi complex? (b) Describe the lipid bilayer arrangement for plasma membrane. (c) List the three major structural elements of Cytoskeleton and give the general function of each. [4+8+4] 5. (a) What are plasma functional enzymes? Explain. (b) How will you carryout diagnosis of Wilsons disease? [6+10] 6. (a) Write about the preparation and characterization of reagents used in Radio Immuno Assay. (b) Dierentiate between nucleotides, nucleosides & organic bases present in nucleic acids. [8+8] 7. (a) What do you understand by blood sugar levels in the body and what is its clinical signicance? (b) Explain one best method for estimation of glucose in a blood sample. [8+8] 8. (a) Name the important techniques used for the measurement of activity of radio isotopes. (b) Describe the important applications of isotopes. 1 of 1 [8+8]

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