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Chapter 4
What is metabolism
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Anabolic reaction
! A chemical reaction where energy is stored in molecules ! Example: photosynthesis
2.!
Catabolic reaction
! ! A chemical reaction where energy is released from molecules Example: Cellular respiration
Oxidation-Reduction Reaction
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Processing of energy by cells which involves a transfer of energy through the flow of electrons
OXIDATION
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REDUCTION
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REDOX Reactions
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An oxidation reaction is always accompanied by a reduction reaction because there are no free electrons in living cells (all electrons are in atoms) If a substance gains an electron in a reduction reaction, that electron must come from another substance that has lost an electron
KEY TERMS
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A series of chemical reactions during which hydrogens or their electrons are passed from one acceptor molecule to another, with the release of energy Electron acceptor molecule molecules that temporarily accept the molecule until they are passed on to another acceptor molecure
LEARNING OBJECTIVE 2
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Define photosynthesis Describe how photosynthesis is important not only to plants but to the entire web of life on planet Earth
KEY TERMS
PHOTOSYNTHESIS
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Biological process that includes capture of light energy and its transformation into chemical energy of organic molecules (such as glucose), which are manufactured from carbon dioxide and water
Photosynthetic Organisms
Continuous range of electromagnetic radiation propagated through space and matter Wavelength distance from one wave peak to the next
One wavelength
700 nm
Orange Color spectrum of visible light 600 nm Yellow Green 500 nm Blue Violet
Gamma rays
400 nm 380 nm
Electromagnetic spectrum
Shorter wavelength
(a) Visible light is that portion of the electromagnetic spectrum that consists of wavelengths from 380 to 760 nm.
Fig. 4-2a, p. 69
Sun Sunlight is a mixture of many wavelengths (b) Electromagnetic radiation from the sun includes ultraviolet radiation and visible light of varying colors and wavelengths.
Fig. 4-2b, p. 69
Photon
Photon is absorbed by an excitable electron that moves into a higher energy level.
Either
Or
Electron acceptor molecule The electron may return to ground level by emitting a less energetic photon. The electron may be accepted by an electron acceptor molecule.
Fig. 4-3, p. 69
Light Energy
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Without photosynthesis, there would be no energy for plants, animals, other organisms Humans and other animals depend on plants for food (energy) and oxygen
Palisade mesophyll
Vein Air space Spongy mesophyll Stoma (a) This leaf cross section reveals that the mesophyll is the photosynthetic tissue. CO2 enters the leaf through tiny pores, or stomata, and H2O is carried to the mesophyll in veins. Fig. 4-4a, p. 70
Mesophyll cells
(b) Notice the numerous chloroplasts in this LM of leaf cells. Inner Outer Stroma membrane membrane
Thylakoid membrane
(c) In the chloroplast, pigments necessary for the light-capturing reactions of photosynthesis are part of thylakoid membranes, whereas the enzymes for the synthesis of carbohydrate molecules are in the stroma. Fig. 4-4bc, p. 70
LEARNING OBJECTIVE 3
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Write a summary reaction for photosynthesis, explaining the origin and fate of each compound involved
KEY TERMS
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CHLOROPHYLL
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Chlorophyll a Molecule
Side chain
Fig. 4-5, p. 71
Photosynthesis
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Chlorophyll captures light energy from the sun and uses it to chemically combine hydrogen from water with CO2 from the atmosphere to produce carbohydrates Oxygen is released as a by-product
Overview: Photosynthesis
Light-dependent Reaction
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3.!
4.!
Chlorophyll absorbs light Electrons are excited and move up to a higher energy level The displaced electron is replaced by an electron from water water dissociates to form oxygen Product: ATP and NADPH
Carbon fixation reactions (in stroma) Chloroplast ATP ADP NADPH NADP+
Light reactions
Calvin cycle
H 2O
O2
CO2
Carbohydrates
Fig. 4-8, p. 73
2.!
Uses ATP and NADPH formed from the previous reaction for fuel Carbon atoms are fixed to existing skeletons of organic molecules
LEARNING OBJECTIVE 4
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Summarize the events of the lightdependent reactions of photosynthesis, including the role of light in the activation of chlorophyll Describe how a proton gradient allows the formation of ATP according to the chemiosmotic model
Chlorophyll absorbs light, becomes energized Energy is used to make adenosine triphosphate (ATP) and to split water Hydrogen from water is transferred to nicotinamide adenine dinucleotide phosphate (NADP+), forming NADPH
Thylakoid membrane
Photosystem
Fig. 4-10, p. 74
KEY TERMS
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In photosynthesis, the linear flow of electrons, produced by splitting water molecules, through photosystems I and II, Products: ATP, NADPH, and O2
Primary 2e electron acceptor EA Relative energy level ADP + Pi Production of ATP ATP
H 2O Photosystem II (P680)
Photosystem I (P700)
1 Electrons are supplied to system from the splitting of H2O by photosystem II, with release of O2 as a byproduct. When photosystem II is activated by absorbing photons, electrons are passed along electron transport chain and are eventually donated to photosystem I.
2 Electrons in photosystem I are reenergized by absorption of additional light energy and are passed to NADP+, forming NADPH.
Fig. 4-11, p. 75
Photosystem 2
!
!
ATP is formed
NADPH is not formed
Chemiosmosis
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Synthesizes ATP in both noncyclic and cyclic electron transport using the energy of a proton gradient established across a membrane Protons flow through a membrane channel within the enzyme ATP synthase
Thylakoid membrane
Protons (H+)
Proton gradient
Fig. 4-12, p. 76
KEY TERMS
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ATP SYNTHASE
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An enzyme complex that synthesizes ATP from ADP, using the energy of a proton gradient; located in thylakoid membranes of chloroplasts and in the inner mitochondrial membrane
Photon
ATP synthase
Photosystem I
Cytochrome Complex
Ferredoxin
Stroma
Fig. 4-13, p. 77
LEARNING OBJECTIVE 5
Summarize the events of the carbon fixation reactions of photosynthesis (Light Independent Reaction)
KEY TERMS
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CARBON FIXATION
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A cyclic series of reactions that fixes carbon dioxide and produces carbohydrate
Carbon Fixation
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Most common pathway of carbon fixation Uses energy stored in ATP and NADPH during lightdependent reactions to chemically fix CO2 3 phases:
1.! Carbon dioxide uptake 2.! Carbon reduction 3.! RuBP regeneration
5 carbon sugar turns into a 6 carbon sugar Immediately splits in two 3-carbon molecule called phosophoglycerate
Phosphoglycerate is converted to phosphoglyceraldehyde-3-phosphate (NADPH and ATP) 1 turn of the Calvin cycle produces two G3P
! !
In 6 turns = 12 G3P 2 of the G3P leave the cycle to be used in carbohydrate synthesis (glucose or fructose)
10 of the G3P that remain are rearranged into 6 molecules of the 5-carbon Ribulose phosphate (RP) Another batch of ATP is expended to add phosphate to RP, forming Ribulose biphosphate to be used for another round of the Calvin Cycle
Ingredients:
! ! !
Product
!
Carbohydrate molecule
6 molecules of CO2 6 molecules of ribulose bisphosphate (RuBP) P P 6 ADP 6 ATP 6 molecules of ribulose phosphate (RP) 3 RuBP P regeneration phase CALVIN CYCLE 1 CO2 uptake phase 12 molecules of phosphoglycerate (PGA) P 12 ATP 12 ADP 12 NADPH 12 NADP+ 12 P P 12 molecules of glyceraldehyde-3phosphate (G3P) P 2 molecules of glyceraldehyde-3-phosphate (G3P)
Fig. 4-14, p. 78
Upper epidermis Palisade mesophyll Bundle sheath cells of veins Mesophyll Spongy mesophyll Chloroplasts
(a) In C3 plants, the Calvin cycle takes place in the mesophyll cells, and the bundle sheath cells are nonphotosynthetic.
(b) In C4 plants, reactions that fix CO2 into four-carbon compounds take place in the mesophyll cells. The four-carbon compounds are transferred from the mesophyll cells to the photosynthetic bundle sheath cells, where the Calvin cycle takes place.
Fig. 4-15, p. 79
Summary: C4 Pathway
CO2
Mesophyll cell
Oxaloacetate (4C)
(3C)
(3C) Pyruvate
Glucose
NADPH
CAM Pathway
Epidermis NIGHT
CO2
Stoma open
Epidermis CO2
Malate Malic
acid
Malate Malic
CO2 acid
Sugar
Mesophyll cell
Vacuole Cytoplasm (c) During the day, when stomata are closed, CO2 is removed from malate and becomes available to be fixed into sugar by the Calvin cycle in the chloroplasts (located in the cytoplasm).
Fig. 4-17bc, p. 81
(b) CAM plants open their stomata at night, and CO2 enters. In mesophyll cells, the CO2 is converted into malate, which is stored in cell vacuoles as malic acid.
LEARNING OBJECTIVE 6
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Write a summary reaction for aerobic respiration, giving the origin and fate of each substance involved
KEY TERMS
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AEROBIC RESPIRATION
!
Process by which cells use oxygen to break down organic molecules, with the release of energy that can be used for biological work
Aerobic Respiration
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During aerobic respiration, a fuel molecule such as glucose is oxidized, forming CO2 and water, with the release of energy stored in the bonds of ATP molecules
1 Glycolysis
2 ATP
2 ATP
32 ATP
Fig. 4-18, p. 82
LEARNING OBJECTIVE 7
!
List and give a brief overview of the four stages of aerobic respiration
Glycolysis Formation of acetyl CoA Citric acid cycle Electron transport system (with associated chemiosmosis)
Glycolysis
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Glucose is split into two molecules of pyruvate with production of a small amount of ATP Hydrogen atoms removed from glucose are transferred to nicotinamide adenine dinucleotide (NAD+), forming NADH
Two pyruvate molecules each lose a molecule of CO2 Remaining acetyl groups combine with coenzyme A, producing acetyl coenzyme A One NADH is formed as each pyruvate is converted to acetyl CoA
Each acetyl CoA enters the citric acid cycle by combining with a 4-carbon compound to form citrate, a 6-carbon compound With two turns of the citric acid cycle, two molecules of acetyl CoA are completely degraded, four CO2 molecules are released
Hydrogens are transferred to NAD+ and FAD, forming NADH and FADH2 Only one ATP is produced directly with each turn of the citric acid cycle
Electron Transport
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Hydrogen atoms (or electrons) from fuel molecules are transferred from one electron acceptor molecule to another Final acceptor in the chain is O2, which combines with the hydrogen to form water
Chemiosmotic Model
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Energy liberated in the electron transport chain is used to establish a proton gradient across the inner mitochondrial membrane Protons flow through a membrane channel within the enzyme ATP synthase
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KEY TERMS
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GLYCOLYSIS
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The first stage of cellular respiration, in which glucose is split into two molecules of pyruvate with the production of a small amount of ATP
KEY TERMS
!
A series of aerobic chemical reactions in which fuel molecules are degraded to carbon dioxide and water, with the release of metabolic energy used to produce ATP
KEY TERMS
!
CHEMIOSMOSIS
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The synthesis of ATP using the energy of a proton gradient established across a membrane; occurs during electron transport in both photosynthesis and aerobic respiration
Proton Gradient
Fig. 4-19, p. 83
LEARNING OBJECTIVE 8
Fermentation
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Anaerobic pathway that degrades glucose and other organic molecules without oxygen Uses glycolysis, but final acceptor of hydrogen is an organic molecule
Alcohol fermentation
! !
In yeasts, certain others Degrades glucose to pyruvate, then splits CO2 off, forming ethyl alcohol
Lactate fermentation
! !
Fermentation
Fig. 4-21a, p. 87
2 ATP
2 Pyruvate
CO2 2 Ethyl alcohol (b) In alcohol fermentation, the twocarbon ethyl alcohol is the end product. 2 Lactate (c) In lactate fermentation, the final product is the three-carbon compound lactate.
Fig. 4-21bc, p. 87
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