APPENDIX 9

Procedure for Spray Test of Triazine Tolerant Canola

Updated Dec 2013

MATERIALS

Equipment: 2L Sprayer (calibrated)

Chemical: Gesprim (or similar chemical)
Active constituent: 600g/L Atrazine

Potting Mix: Available from research bunker

Seed: Representative sample of line being tested
Susceptible line (ie. susceptible control)
Tolerant line that has been tested and passed (ie. tolerant control)

Trays: 2 x 100 cell grow trays (for variety to be tested)
1 x 100 cell grow tray ( for susceptible control, for tested & passed line)

Herbicide rate:
Mix 5ml chemical/250ml water

Sprayer: Pump to maximum pressure

METHOD

Using potting mix, fill all trays.
Sow a number of seeds from each line into each cell of relevant trays, water lightly and
grow using normal horticulture practices.
Label trays with: Variety, batch, grower, date planted, name of test (TT).

When seedlings appear, thin cells, allowing for attrition i.e. keep a number of extra plants to
achieve correct population.
Before spraying remove extra plants, leaving 100 plants per tray.

Plants should be sprayed at two to four leaf stage.
Align trays to be sprayed lengthwise in suitable area for spraying.
Apply herbicide to dry foliage holding spraying wand at height to produce a band width
of 32cm.
Spray trays walking at medium pace with one sweep.
Label trays with date sprayed and name of test (TT).

ASSESSMENT

Triazine Response takes approximately 7 days or less.
Symptoms of the susceptible variety will be rapid and include severe wilting and plant
mortality. Response in the susceptible seedlings will be dramatic and clearcut.

Record results in QC database and other relevant files.

If results obtained from the spray test differ from what is expected, note it in the QC
database and refer to the Genetic Purity Manager immediately to discuss further action.