Expression of metallothionein protein in feline mammary tumors by using

tissue microarray technique

Panchan Sitthicharoenchai
1
, Phimonrat Marlow
1
, Perapux Prutthithaworn
1
,

Sudson Sirivaidyapong
2
, Supradit Wangnaithum
1
,

Sirikachorn Tangkawattana
3
,
nudep !ungsipipat
1"
1.
#epartment o$ %eterinary Pathology, &aculty o$ %eterinary Science, 'hulalongkorn
(niversity, )angkok 1*33* Thailand
2.
#epartment o$ %eterinary Medicine, &aculty o$ %eterinary Science, 'hulalongkorn
(niversity, )angkok 1*33* Thailand
3.
#epartment o$ %eterinary Patho+iology, &aculty o$ %eterinary Medicine, ,hon ,aen
(niversity, ,hon ,aen, Thailand
Background/Aim
Metallothionein -MT. is a protein $ound in many parts o$ +ody and has +een studied and $ound
related to many types o$ tumor including $eline mammary gland tumor -&MT./ The purpose o$
this study was to $ind the relations o$ MT to the histopathological types and histological grades
o$ &MT +y using tissue microarray techni0ue/

ethods
The expression o$ P'1, ,i234 and MT21 were detected using immunohistochemistry in 4*
cases o$ &MT/ The &MT were classi$ied into 5 histopathological types6 tu+ular adenocarcinoma
-T'., papillary adenocarcinoma -P'., cri+ri$orm carcinoma -''. and solid carcinoma -S'.,
and 3 histological grades6 7, 77, and 777/
!esults
The P'1 index $ound in T', P', '', and S' were 38/83913/22, 2:/429:/:8, 33/::912/8*,
and 3;/83914/;8, respectively/ 7n di$$erent histological grades, P'1 index were 51/4;9:/4: -7.,
33/85912/84 -77., and 3;/28918/44 -777./ The ,i234 index $ound in T', P', '', and S', were
34/8;91;/;;, 2;/5:912/*5, 3:/:*924/2*, and 58/55915/*3, respectively/ 7n di$$erent histological
grades, ,i234 index were 13/1:911/38 -7., 35/8491;/*8 -77., and 5;/14913/33 -777., with
signi$icant di$$erences -p0.05. $ound within the histological grades, +ut not in the
histopathological types/ The expression o$ MT21 were $ound in all the 4* cases and the percent
positive area in T', P', '', and S' were 55/1393*/25, 3:/2:923/21, 22/33914/;1, and
3;/1293*/33, respectively/ 7n di$$erent histological grades, MT21 percent positive areas were
32/3;924/8: -7., 34/35923/34 -77., and 53/85932/:4 -777./ The results showed no signi$icant
di$$erences in MT21 expression within the histopathological types and the histological grades/
"onclusion
The expression o$ MT21 cannot $ully indicate the invasiveness o$ the &MT in terms o$
histopathological typing, however, with higher histological grades, the more percent positive
areas o$ MT21 were $ound/

#ey$ords% $eline mammary tumor, immunohistochemistry, metallothionein, tissue microarray
techni0ue