What other name can be given to a nucleotide?

A. Glycosylated nucleoside
B. Purinated pentose
C. Deoxyribonucleotide
D. Nucleoside phosphate
E. Pyrimidinate

Which of the following methods is most like microarray

technology?

A. Gel-filtration chromatography
B. Ramachandran plotting
C. Western blotting
D. SDS-polyacrylamide gel electrophoresis
E. Sanger protein sequencing

Some effort has been made to extract DNA from

fossilized dinosaur bones that have been buried for
many millions of years in arid climates. In what form is
this DNA most likely to be found?
A. A
B. B
C. Z
D. Tetraplex
E. Completely denatured

What name is given to the nucleotide found in DNA

and not in RNA?
A. Uridylate
B. Thymidine
C. Uracil
D. Deoxythymidylate
E. Deoxythymidine

What function is served by the major groove in DNA?

A. It is the binding site for ethidium bromide.
B. It is a binding site for regulatory proteins.
C. It is a region of denatured DNA to which
restriction endonucleases bind.
D. It is a region of DNA that can form a hybrid
duplex with DNA of another species.
E. It is the site of guanine tetraplex formation.

Which structure is found in RNA, but would be the

result of a mutation or faulty annealing in DNA?

A. Hairpin
B. Double-stranded helix
C. Cruciform
D. Sequence complementation
E. Bulge

A graduate student carefully heated DNA to about 80C

and then prepared it for electron microscopy. She
observed a number of small open loops of DNA. What
conclusion did she correctly make from her observation?
A. The open loops contain DNA mutations, such as
cyclobutane dimers and deaminations.
B. The denatured DNA is the site of genes, of coding
regions, and the annealed DNA is the site of noncoding DNA.
C. The open loops contain DNA with a high A-T
content.
D. The open loops contain denatured DNA that is
being transcribed.
E. The open loops are plasmids, and the annealed
DNA is genomic.

Sanger sequencing was performed on DNA from

chloroplasts of a new plant species discovered in the
rain forest of Puerto Rico. The X-ray film showed
bands in the A, C, and T lanes, but the G lane was
blank. What is the most likely explanation for this
unexpected result?
A. ddG was not added to the reaction tube.
B. Different template DNA was added to the
reaction tube.
C. The template DNA contained no G.
D. All 4 ddNTPs were added to that tube.
E. The template DNA contained no C.

Which of the following is not a nucleotide?

A. Coenzyme A
B. FADH2
C. ATP
D. Anti-cytidine monophosphate
E. All of the above are nucleotides.

Telomeres are non-coding sequences on the ends of

chromosomes. They contain a high concentration of
guanosine. Given this information, what other
structure may be in telomeres?
A. Cyclobutane dimers
B. A high concentration of triplexes
C. Cruciform
D. Tetraplexes
E. A-form DNA

Which positions in the purine ring of a purine

nucleotide in DNA have the potential to form
hydrogen bonds but are not involved in WatsonCrick base pairing?

Explain why the absorption of UV light by

double-stranded DNA increases (the
hyperchromic effect) when the DNA is
denatured.

Solubility of the Components of DNA Draw the

following structures and rate their relative solubilities
in water (most soluble to least
soluble):deoxyribose, guanine, phosphate. How are
these solubilities consistent with the threedimensional structure of double-stranded DNA?