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Introduction
Structure of Molecule
- A terameric conjugated chromoprotein having
heterogeneous quaternary structure
A respiratory pigment of animal blood; meant for transporting O2 (oxyhaemoglobin) and CO2 (carbomaenohaemoglobin) as
respective loose complexes; forms irreversible permanent complex (carboxyhaemoglobin) with CO, effecting its O2
transportation capacity
Its empirical formula is C3032H9816O872N780S8Fe4
Its molecular structure was given by Kendrew & Perutz(), based on X-ray diffraction studies
Size 64 X 55 X 50
Haem Molecules
-
4 in number
Non-protein prosthetic groups
Each consists of 4 Pyrolle rings
with 1 central Iron atom
Each Haem can attach to 1
molecule of O2; therefore, all
together can transport 4O2
molecules
Pyrolle Rings
-
Iron Atoms
-
Globin Molecules
-
Functions
4 in number (I - IV)
Empirical formula is C20H14N4
N containing heterocyclic rings with specific side chains
CH3 (at positions 1,3,5 & 8)
CHCH2(at positions 2 & 4)
(CH2)2COOH ((at positions 6 & 7)
Pyrolle rings linked by methine(-CH=; methylene) bridges (at positions , , & )
2+
Polypeptide Chains
-
2 in number
Each consists of 141 Amino acids with valine at N-terminus and arginine at Cterminus
th
Haem pocket formed by histidine at 87 position
th
Binding site for Fe of Haem at 58 position histidine
Polypeptide Chains
-
2 in number
Each consists of 146 Amino acids with valine at N-terminus and histidine at Cterminus
nd
- Haem pocket formed by histidine at 92 position
rd
- Binding site for Fe of Haem at 63 position histidine
Transport O2 from respiratory surface to various tissues as a loose complex oxyhaemoglobin (Hb 4O8; Hb (O2)4); In mammals,
97% of O2 is transported in this form; O2 is attached to Haem part of Hb
Transport of CO2 from tissues to respiratory surface as a loose complex carbomaenohaemoglobin (HbNHCOOH). In
mammals, accounts for 23% of CO2 transported in the blood. CO2 is attached to Globin part of Hb.
+
Serves as an Acid-base buffer, as the constituent amino acids of the Globin either due to negative charge (which binds to H
+
ions) or ionization (e.g., Histidine produce H ions to neutralize alkalinity)