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  • KtbtAFfL'M Amu rNVUVt. I Ucv Eivrnscw A

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    isomerase
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    Glucose isomerase activities were routinely obtained in 600 to 700 40 / mi in an inmcpensive medium. Conditions for production of 41 to 43% fructose syrups have been evaluated. Medium modifications are being evaluated to increase enzymatic activities. A 500-pound (DWB) of syrup will be produced and cleaned up for 90-day toxicity studies. Rats and dogs will be used as test animals.

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    Glucose isomerase activities were routinely obtained in 600 to 700 40 / mi in an inmcpensive medium. Conditions for production of 41 to 43% fructose syrups have been evaluated. Medium modifications are being evaluated to increase enzymatic activities. A 500-pound (DWB) of syrup will be produced and cleaned up for 90-day toxicity studies. Rats and dogs will be used as test animals.

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    11 views2 pages

    KtbtAFfL'M Amu rNVUVt. I Ucv Eivrnscw A

    Uploaded by

    isomerase
    Glucose isomerase activities were routinely obtained in 600 to 700 40 / mi in an inmcpensive medium. Conditions for production of 41 to 43% fructose syrups have been evaluated. Medium modifications are being evaluated to increase enzymatic activities. A 500-pound (DWB) of syrup will be produced and cleaned up for 90-day toxicity studies. Rats and dogs will be used as test animals.

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    of 2

    KtbtAFfL'M Amu rNVUVt.

    I ucv eivrnscw a
    . PAGE__1 _ OF _,2
    50197 7333 PROI/ECT STATUS REPORT
    uI. G .
    OOUtPANY: Penick and Pord . Ltd.
    FOR PERIOD ENDING Auf[ust 31 79 fil-
    *U4~L"
    DIVISION: Bioloaical Research

    ISSUED BY : Eldon D . Nielson


    .w.u
    ~ EXPENDITURES YEAR TO
    rROJECT TITLE AND DEBCRIMTION AAMIUAL DATE
    sTATUS ACTION
    BUDGET % OF
    MqK~ Mom~ Yw m Dsoe
    NQ OUDGET

    132 Biochemical, Synthesis Glucose isomerase activities were Medium modifications are being
    routinely obtained in 600 to 700 evaluated to increase enzymatic
    40/mi in an inmcpensive medium . activities .

    Conditions for production of 41 to Optimization of these processes


    43% fructose syrups have been to produce high fructose syrups
    evaluated . are under investigation .

    Preliminary procedures for purifi- A thorough study of cell lysis


    cation of 41 to 43Z syrups have and cell bleed is under investi-
    been determined . gation to determine the color-
    producing species and, hence,
    aid in purification .

    Small volumes (30 gallons) of A 500-pound (DWB) of syrup will


    41 to 43% fructose syrup have be produced and cleaned up for
    been produced for purification 90-day toxicity studies for
    studies . Cells have been produced self-certification of this
    for feeding-trial . process . Rats and dogs will be
    used as test animals .

    Physiological characteristics of Further observation of the taxo-


    mutant organisms used to produce nomic characteristics and basic
    glucose isomerase were determined cultural behavior patterns of
    for the patent application . the three mutant strains will be
    determined .

    134 Fermentation Development Fermentation scale-up parameters, The fermentation process is


    such as pH, dissolved oxygen, and being optimized with respect 'to
    agitation, were determined for the the .scale-up parameters .
    glucose isomerase fermentation .

    .
    f
    0 RESEARCH AND PRODUCT DEVELOPMENT
    PROJECT STATUS REPORT 50497 7334 PAGE__Z_ OFZ_
    FOR PERIOD ENDiNG Auaust 31 t669
    COMPANY : Peniek and Pnrd- Ltd_

    DryISIpN : Biolottical Research

    ISSUED BY: Eldon D. Nielson


    R .p~ra
    Na . EXPENOITURES YEAR
    AR TO
    .ROJECT TITLE AND DESCRI~TION STAINB ACTION ~~~ OATE
    pq.~t
    ~ Mon~ Y.r eo Ou. WDGET % OF
    il
    T

    135 Starch Biochemistry Reversion has been observed with Project terminated .
    all glucoamylases, hence, it
    appears Impossible to produce
    "pure" glucose from starch
    enzymatically .

    Thin-layer chromatography is not High temperature chromatog-


    satisfactory for determining the rapby is being evaluated
    suitability of commercial enzyme for routine use in a quality
    samples for quality control . control laboratory.

    A new'procedure for the deter- A report on this procedure


    mination of unfermentable carbo- is in preparation and will
    hydrates in syrups has been be distributed to interested .
    developed . personnel at RJR and Penick
    and Ford .

    i
    927 G-B Development Penaweet chronic (90-day) llnimals will be sacrificed
    toxicity study . 9/2/69 and 9/3/69 . Gross
    pathology, body weights, food
    intake, and blood chemistry
    will be finished by 9/12/69 .

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