Exercise 2: Skeletal Muscle Physiology: Activity 2: The Effect of Stimulus Voltage on Skeletal Muscle Contraction Lab

Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. Skeletal muscle fibers are innervated (stimulated) by
You correctly answered: c. motor neurons.
2. A single action potential propagating down a motor axon results in
You correctly answered: d. a single action potential and a single contractile event in the muscle fibers it innervates.
3. In resting skeletal muscle, calcium is stored in
You correctly answered: c. the sarcoplasmic reticulum.
4. During the latent period for an isometric contraction
You correctly answered: c. the cellular events involved in excitation-contraction coupling occur.

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Experiment Results
Predict Question:
Predict Question: As the stimulus voltage is increased from 1.0 volt up to 10 volts, what will happen to the amount of active
force generated with each stimulus?
Your answer : a. The active force will continually increase.
Stop & Think Questions:
What do you see in the active force display when the stimulus voltage is set to 0.0, and why does this observation make
sense?
You correctly answered: a. 0.00 g; there was no activation of skeletal muscle fibers by this stimulus.
What is the lowest stimulus voltage that induces active force in the skeletal muscle?
You correctly answered: b. threshold voltage
6. Enter the threshold voltage for this experiment in the field below and then click Submit to record your answer in the lab
report
You answered: 0,8 volts
12. Enter the maximal voltage for this experiment in the field below and then click Submit to record your answer in the lab
report.
You answered: 10,0 volts
Experiment Data:
Voltage
0.0
0.2
0.8
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0

Length
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75

Active Force
0.00
0.00
0.02
0.15
0.43
0.66
0.87
1.04
1.19
1.32
1.42
1.51
1.59
1.65
1.70
1.74
1.78
1.81
1.82
1.82
1.82
1.82

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Passive Force
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

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Total Force
0.00
0.00
0.02
0.15
0.43
0.66
0.87
1.04
1.19
1.32
1.42
1.51
1.59
1.65
1.70
1.74
1.78
1.81
1.82
1.82
1.82
1.82

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Post-lab Quiz Results

You scored 80% by answering 4 out of 5 questions correctly.
1. Motor unit recruitment refers to
You correctly answered: a. an increase in the number of active muscle fibers to increase the force developed in a muscle.
2. Active tension (or force) in a skeletal muscle fiber results from
You correctly answered: a. activation of cross bridge cycling via increased intracellular calcium levels.
3. The ________ is the minimal stimulus needed to cause a depolarization of the muscle plasma membrane (sarcolemma).
Your answer: c. latent voltage
Correct answer: d. threshold voltage
4. By definition, the ________ is the amount of stimulus required to successfully recruit all the muscle fibers into
developing active force.
You correctly answered: c. maximal voltage
5. Why was a maximal voltage observed in this experiment?
You correctly answered: b. At the maximal voltage, all the muscle fibers contained in this muscle are depolarized and they
all develop active force (that is, they were all successfully recruited).

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Review Sheet Results

1. Describe the effect of increasing stimulus voltage on isolated skeletal muscle. Specifically, what happened to the muscle
force generated with stronger electrical stimulations and why did this change occur? How well did the results compare with
your prediction?
Your answer:
Sesuai dengan praktikum yang telah dilakukan maka kita dapat membuktikan bahwa ketika diberi stimulus yang lebih tinggi
maka active force-nya juga akan semakin tinggi.Dimana setiap voltage ditingkatkan maka active force-nya juga akan
meningkat.Tetapi dari percobaan kita juga harus memperhatikan panjang otot.
2. How is this change in whole-muscle force achieved in vivo?
Your answer:
Jumlah serat yang bekontraksi di suatu otot bergantung pada tingkatan rektumen unit motorik.Dimana semakin banyak
jumlah serat otot yang aktif maka semakin besar tegangan otot itu sendiri.Keseluruhan jumlah serat serat yang berkontraksi
bergabung pada :
1. Ukuran otot (jumlah serat otot yang ada)
2. Tingkat rektumen unit motorik
3. Ukuran masing masing unit motorik.
3. What happened in the isolated skeletal muscle when the maximal voltage was applied?
Your answer:
Berdasarkan percobaan yang telah dilakukan, apabila otot rangka diberikan stimulus volta semaksimal mungkin yaitu 10,0
maka yang akan terjadi adalah meningkatnya active force sehingga mencapai 1,82. Tetapi hal ini disesusaikan juga dengan
panjang otot, dalam hal ini panjang otot adalah 75 mm dan apabila diberikan voltage lebih dari 10,0 maka dapat
menimbulkan abnormal atau kelainan pada otot.

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