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S2214-8604(15)00014-7
http://dx.doi.org/doi:10.1016/j.addma.2015.03.001
ADDMA 31
To appear in:
Received date:
Revised date:
Accepted date:
21-9-2014
2-2-2015
5-3-2015
Ehsan Toyserkani1
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Mihaela Vlasea1
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1-University of Waterloo, Department of Mechanical and Mechatronics Engineering, Waterloo, Ontario, N2L 3G1
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2-Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada M5S 3G9
Submitted to:
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Number of Pages:
21
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Submission Date:
Number of Figures:
Number of Tables:
Contact Author:
Address:
Email:
mlvlasea@uwaterloo.ca
Phone:
1-519-722-1368
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osteochondral defects. In this study, the three dimensional printing (3DP) AM process was
investigated to determine the effect of powder layer orientation on mechanical and structural
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properties of fabricated parts. Five types of standard cylindrical parts were manufactured via AM
with 0, 30, 45, 60 and 90stacking layer orientations relative to the vertical z-axis of the print
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bed, using amorphous calcium polyphosphate (CPP) powder of irregular particle shape, average
aspect ratio 1.70 and particle size between 75-150 m. It was concluded that layer orientation
had an effect on porosity and compressive strength, based on induced powder particle orientation
in the green part during powder layering. The resulting bulk porosity values ranged between 30.0
2.4% to 38.2 2.7%, while the compressive strength ranged between 13.50 1.95 MPa to
45.13 6.82 MPa. The orientation with the highest compressive strength was 90, while
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orientations with the weakest compressive strength were 0 and 45. Based on these results, it
was established that AM-made parts are structurally and mechanically anisotropic. The stacking
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layer orientation which results in the highest strength performance along a preferred loading
orientation can be implemented to further optimize mechanical strength of constructs along the
maximum loading direction.
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Introduction
Porous bone substitutes serve as an artificial matrix providing the mechanical and structural
template for new bone formation. Such porous structures must be biocompatible and ideally
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should promote osteogenesis by being osteoconductive and osteoinductive while degrading invivo at an appropriate rate to allow their replacement by newly-formed bone [1,2]. The porous
structure must also be designed to have an anatomically accurate three dimensional (3D) shape
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in order to maintain a natural contact load distribution post implantation [3] and initial internal
structure in terms of micro- and macro-interconnected porosity to promote cell proliferation,
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metabolic exchange and vascularisation [4]. Furthermore, the mechanical strength and porous
architecture of the bone substitute should ideally be designed to match the load bearing
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requirements at the substitution site and to promote the appropriate bone growth cues as dictated
by mechanostat theory [5]. Ideally, this would suggest anisotropic structural and mechanical
promising fabrication method for making scaffolds or porous constructs in the field of tissue
engineering and regenerative medicine, and specifically for bone substitute fabrication [2,610].
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Using this approach, the anatomical shape and internal porous configuration of the implant is
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first designed in a computer-aided design (CAD) environment. Subsequently, the CAD model is
converted into image slices and the scaffold is manufactured in a layer-by-layer fashion by
repeated stacking powder layers and subsequently injecting a binder solution at locations dictated
by the cross-sectional image of the part layer to be formed as shown in Figure 1. The resulting
product is referred to as a green part. For ceramic structures, such as the calcium polyphosphate
(CPP) used in the present study, further post-green part processing, usually involving thermal
annealing, is necessary to achieve required strength properties and structural characteristics (i.e.
% porosity, pore size and interconnectedness) of the final part [4,8].
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Prior investigations by a number of groups have focused on fine-tuning 3DP process parameters
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to examine their effect on mechanical and structural properties of the final product. The
appropriate binder-powder material systems compatible with both the additive manufacturing
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process and the biological requirements of the bone substitute were investigated [2,11]. Other
studies have examined the effect of the liquid binder used during powder lay-up and green part
formation with regard to its chemical composition [12,13], concentration [10,12,13] and
saturation levels [1416] to determine appropriate binder-powder interactions that would
produce samples with a desired compressive and flexural strength and porosity [10,12,13,15].
Other studies have focused on defining powder composition and blends to yield better powder
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flow characteristics [17,18] and improved mechanical performance of the final structure [12].
The effect of powder particle size and its effect on physical, structural and mechanical properties
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of AM-formed constructs has also been studied [19]. Layer thickness is another parameter that
can be controlled during the 3DP process, with a range of layer thicknesses having been used for
preparation of samples in order to study the effect on mechanical properties [15]. These studies
concluded that, in general, the flexural and compressive strength performance is inversely
proportional to layer thickness [15,20]. The effect of including open or closed macro-channels
within the porous structures during the 3DP processing and its effect on mechanical strength and
biological response of porous constructs has also been studied [12,19,2124]. In this context, a
new type of 3DP platform has been investigated, capable of creating interconnected macrochannels with a feature size below 500 m within the part while avoiding the risk of having
particles trapped within the macro-channels [25,26].
In 3DP, due to the nature of the layer-by-layer manufacturing process, the effect of layer
stacking orientation within the part may influence the physical, structural and mechanical
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properties of constructs so formed. Shanjani et al. [27] and Zhang et al. [20] studied the effect of
layer orientation along the direction of the printing axes and concluded that mechanical strength
characteristics were related to orientations used in forming parts. This effect has not been
explored in detail, as the two previously reported studies focused only on orientations along the
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printing axes z, y, z, without considering intermediate orientations. In the present study, to better
understand the correlation between layer orientation and mechanical properties, standard
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cylindrical parts with 0, 30, 45, 60, and 90 layer stacking orientations with respect to the vertical
axis (z-axis) in the build chamber were fabricated and characterized in terms of porosity, bulk
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density, and compressive strength. It is proposed that the stacking layer orientation within a part
which results in the highest strength can be aligned during part fabrication in the direction of
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anticipated maximum loading, if this is known during the design stage. Or, contrarily, the
orientation resulting in the lowest strength can be avoided from coinciding with expected high
Materials
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2.1
load-carrying directions.
In this study, the powder material used in the AM process was amorphous calcium
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polyphosphate (CPP) powder formed as reported in earlier studies [28]. The powder is
characterized by an irregular particle shape, an aspect ratio 1.70 and particle size range
between 75-150 m. This powder was mixed with polyvinyl alcohol (PVA) powder (Alfa Aesar,
Ward Hill, MA) of particle size < 63 m at a composition ratio of 90 wt% CPP and 10 wt%
PVA. To ensure a homogeneous blending, the CPP and PVA powders were mixed for 4 hours
using a rotating jar mill (US Stoneware, OH). The PVA powder served as an additional binding
agent in combination with the liquid binder (ZbTM58) (3D Systems, Burlington, MA) which also
served as an aqueous solvent for the PVA particles to give acceptable green strength to samples
formed by 3DP.
2.2
Sample Fabrication
Previous studies reported on preparation of porous CPP samples and the effect of CPP powder
size and method of porous construct preparation have been reported for conventional gravity
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sintering and AM using 3DP [11,27]. In vivo studies of samples so-made have been reported
[29,30].The focus of the present study is the determination of how the layer orientation affects
mechanical strength characteristics of samples. To achieve this, cylindrical test samples of 4 mm
diameter and 6 mm height were manufactured with layer orientations at 0, 30, 45, 60, and 90
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with respect to the vertical axis in the build compartment as seen in Figure 2. A 3DP machine
(ZPrint 310 Plus, 3D Systems, Burlington, MA) was used to manufacture the parts in a layer-by-
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layer fashion. The layer thickness was selected to be 175 m with the AM layer-by-layer powder
spread process being undertaken at a 38C. The cylindrical test parts were designed using
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computer-aided design (CAD) software (SolidWorks Corp., Concord, MA) and imported into the
3D printing software (ZPrintTM) in stereolithography (STL) file format. The cylindrical test parts
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were then oriented within the build bed, as seen in Figure 2, with n=10 parts prepared for each
orientation. The green parts were then air-annealed (Lindberg/Blue M, ThermoScientific) with a
50% R.H. in-furnace environment using a pre-established heat treatment protocol [31]. The
annealing cycle used has been reported elsewhere [11,31], with a heat-uprate of 10C/min from
room temperature to 400C, 2 h dwell at 400C to burn off organic binder constituents, continued
heat-up at 10C/min to 500C and then5C/min to 630C, hold for 1 h (this is the so-called Step-
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1 sinter [31]),and then increasing the temperature to 950C at 10C/min and holding at 950C for
1 h (to achieve complete CPP crystallization and final microstructural development [31]).
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Figure 2Parts printed with 0, 30, 45, 60, 90 layer orientation, arranged in the build compartment.
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2.3
Porosity Characterization
The bulk porosity of the sintered cylindrical samples was determined as described previously
[11] by ethanol displacement using Archimedes principle (ASTM C373 standard). An ethanol
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bath kit (Sartorius YDK01 Density Determination Kit, Sartorius AG, Goettingen, Germany) and
a precision micro-scale balance (APX-203, Denver Instrument, Bohemia, NY, US) were used to
of each specimen. Each specimen was then immersed in
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ethanol and sonicated (VWR Ultrasonics Cleaner B2500A-DTH, VWR International, West
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Chester, PA, US) for one hour at 30C and soaked for another hour. Subsequently, the weight of
the specimen suspended in ethanol
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ethanol, dabbed with a lint free cloth to remove excess ethanol and weighed immediately after in
. The bulk porosity
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determined based on the formulae below, (Equations 1 and 2), where the density of ethanol
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at room temperature equals 0.785 g/cm3 and the theoretical density of non-porous CPP
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equals 2.850 g/cm3[28]. A population of n=10 samples was used for this data.
2.4
(1)
(2)
Structural Characterization
The microstructure of final sinter-annealed cylindrical CPP samples with different layer
orientations was examined using secondary electron emission scanning electron microscopy
(SEM, JSM-6460, Jeol, Akishima, Tokyo) operating at 20 kV accelerating voltage. In
preparation for SEM examination, the samples were sputter-coated with a 10 nm thick gold layer
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to make them electrically conductive (Desk II, Denton Vacuum, LCC, Moorestown, NJ, USA).
One representative sample from each category was considered for SEM analysis.
2.5
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The powder size and aspect ratio were determined by placing a thin layer of powder on a
conductive substrate and using secondary electron emission scanning electron microscopy (SEM,
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JSM-6460, Jeol, Akishima, Tokyo) at 20 kV accelerating voltage to view particle images. Five
images were captured at 100x magnification and three at 200x magnification. The particle
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dimension, across the longest orientation (length) and along the dimension perpendicular to this
direction (width), were recorded using the SEM AnalySIS tool in order to estimate particle
2.6
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aspect ratios.
Uniaxial Compression Characterization
Eight samples (n=8) for each different layer orientation were tested in uniaxial compression.
Testing was conducted on dry samples at room temperature using a 1-kN load cell at a loading
Statistical Analysis
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Volume % porosity (denoted hereafter as bulk porosity), bulk density of the porous structure, and
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compression strength data are reported as means and standard deviation. A one-way ANOVA
single factor analysis of variance was used to evaluate the statistical significance of
measurements, followed by Tukey-Kramer post hoc pairwise comparisons to identify the socalled honest significant differences (HSD) between classes of samples using STATISTICA
V12 (StatSoft, Tulsa, OK) with p<0.05 identifying a significant difference. The ASTM C123907 standard was used to analyse and report the performance of ceramics under uniaxial
compression that failed in a brittle fashion. The Weibull distribution described in Equation 3
was used to predict the probability of failure of the ceramic part [32] as well as to provide a
statistical means of evaluating the quality of the measured data set:
(3)
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where
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4 was used as a probability estimator for the Weibull linear regression, where is the
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3.1
Results
Structural Characterization Results
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(4)
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Figure 3a), the parallel layer orientations are highlighted by the dashed white lines. Figure 3b)
and Figure 3c) show examples of sinter necks resulting from the post-AM thermal sinter/anneal
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treatments used for sample preparation. It can be seen that the particles are well bonded together,
forming an open-pored structure. The SEM images obtained for the other layer stacking
orientations were very similar in nature.
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Figure 3 Images of a CPP sample with 60 layer stacking orientation as viewed under SEM with a
magnification of a)x22, b) x100, and c) x300
The SEM image analysis revealed the distribution of powder size along the length and width of
particles as shown in Figure 4. The powder had an irregular shape, with a mean aspect ratio
greater than one. The bulk of particles had the longest axis above 150 m (average particle size
of 177 42 m), while the smallest axis normal to this long dimension was on average below
120 m (average particle size of 104 28 m). The average particle aspect ratio was determined
to be 1.70.
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3.3
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Figure 4 a) The particle size distribution for particle size sieved between 75-150 m, (n=105) b)
Scanning electron microscope (SEM) view of powder particles at magnification x250
The bulk density and porosity results of the cylindrical samples with oriented layers at 0, 30, 45,
60, 90 were calculated using the Archimedes method for density determination. The results are
summarized in Table 1. Figure 5 illustrates the bulk porosity measurements for the different
layer orientation samples indicating statistically significant differences. The maximum bulk
porosity occurred at an orientation of 0 and 45, with values of 38.22.7% and 37.6 3.1%
respectively. The minimum bulk porosity corresponded to samples prepared at an orientation of
90, where the bulk porosity value was equal to 30.0 2.4%.
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Table 1 Bulk porosity and bulk density characteristics for cylindrical CPP samples printed with
layer orientations of 0, 30, 45, 60, and 90 respectively, (n=10)
(p < 0.05)
38.2 2.7
32.2 2.8
37.6 2.1
34.0 3.2
30.0 2.4
(p < 0.05)
1.76 0.08
1.93 0.08
1.78 0.09
1.88 0.09
2.00 0.07
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0
30
45
60
90
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3DP
Layer Orient.
3.4
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Figure 5 Bulk porosity characteristics of cylindrical samples with layer stacking orientations 0, 30,
45, 60, and 90 respectively (n=10). *(p<0.05)
The results listed in Table 2 indicate that the 90 layering orientation in samples results in the
maximum compressive strength of 45.13 6.82 MPa, while the samples prepared at 45 and 0
layer orientations displayed minimum compressive strengths (13.43 4.60 MPa and13.50 1.95
MPa respectively).
Table 2 - Uniaxial compression results and Weibull moduli of cylindrical samples printed with layer
stacking orientations of 0, 30, 45, 60, and 90 respectively, (n=8)
AM
Layer Orient.
0
30
45
60
(p < 0.05)
13.50 1.95
20.62 6.23
13.43 4.60
28.19 2.46
(p < 0.05)
6.95 (0.91)
3.14 (0.93)
3.18 (0.80)
11.98 (0.95)
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90
45.13 6.82
7.02 (0.90)
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Figure 7 Weibull failure probability functions and linear interpolations for compressive strength of
cylindrical samples printed with layer stacking orientations 0, 30, 45, 60, and 90 respectively
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Figure 8 Fracture surface propagation of samples with orientations 0, 30, 45, 60, and 90
respectively. These images are representative of more than 50% of the samples in each category
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The samples were analyzed qualitatively under the microscope to view the orientation of the
fracture path after compression testing to failure. From representative illustrations shown in
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Figure 8, for samples with 30, 45, 60, and 90, the failure occurred, not surprisingly, along
planes parallel to the stacked powder layers. For the 0 orientation, fracture resulted in an
Discussion
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In this study, the optimization of the additive manufacturing process focused on establishing an
orientation that would yield improved mechanical strength characteristics under given loading
conditions, while providing the interconnected porosity required for implant stabilization by
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bone ingrowth throughout a bone substitute. Five categories of test samples, 4 mm in diameter
and 6 mm in height, were fabricated, with different layer orientations at 0, 30, 45, 60, and 90
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with respect to the vertical z -axis in the build compartment (Figure 2). Experimental results
showed that layer orientation had a statistically significant impact on porosity and compressive
strength of samples. The results showed that the 45-oriented samples had the lowest
compressive strength, (13.43 4.60 MPa), while the 90-oriented samples showed the highest
compressive strength, (45.13 6.82 MPa). This considerable difference in strength corresponded
to differences in porosity, whereby the 45-oriented samples had a high bulk porosity value (37.6
2.1%), while the 90 orientation samples, displaying the highest strength, had the lowest
porosity (30.0 2.4%). It is expected that higher porosity would reduce the overall mechanical
strength of a sample.
The difference in compressive strength can be attributed to the additive manufacturing process,
where the orientation of the irregular-shaped CPP particles within each powder layer is
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influenced by the action of the counter-rotating roller as described by Shanjani et al. [27]. The
results shown in Figure 4 illustrate that the CPP powder used in this work has an irregular shape,
with one distinct longer axis. The counter-rotating roller compacts the powder and aligns the
irregular-shaped particles having an aspect ratio larger than 1 with the longest axis generally
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parallel with the build plane. As reported elsewhere [27], the resulting particle-particle packing
favors inter-particle contacts at particle ends within the build planes (i.e. at smaller radius of
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curvature particle profile contacts, with sharper contact zone). The sinter necks are stronger
when the contact profile between particles has a sharper contact zone, where the contact angle
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between particles is larger, therefore the sinter necks between particles within each build plane
(parallel to xy) are expected to be stronger than the sinter necks between build planes (along z
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axis) [27,34].
shear stresses will act at 45 to the applied force direction resulting in shear fracture along this
direction characterized by an oblique fracture path of failed samples. The observed fracture path
for the 45-oriented samples follows this direction which also corresponds to the weakest sinter
neck direction (i.e. that corresponding to larger radius of curvature contact points between
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particles in general). These are also the lowest fracture strength samples. The 30- and 60oriented samples approach this condition and in view of the irregular particle shapes, their
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measured lower strengths may be similarly explained. Fracture path directions shown in Figure 8
correspond closely to these weakest sample plane directions. This is further expanded on as
discussed below using stress tensor equations summarized in Table 3. In contrast, the 90
orientation corresponds to the weakest direction being parallel to the applied force direction, so
that crack propagation and failure is likely along this direction. The 0-oriented samples develop
highest normal stresses along the weakest planes (Table 3) resulting in transverse fractures (or
more likely, complex fracture paths with both transverse and oblique fracture path segments).
This is reflected in the observed fractures displayed by these samples. In porous ceramic
structures such as CPP, sinter necks represent stress concentration sites, therefore the stronger
the sinter neck, the better the resistance to crack propagation. This means that structurally, the
parts fabricated via additive manufacturing are orthotropic parallel and normal to the z- build
directions.
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Stress tensor theory for uniaxial compression [35] allows shear stress and normal stress at an
orientation
to be computed based on Equation 5 and Equation 6, where the only load acting on
, also denoted by
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(5)
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(6)
The shear stress and normal stress computed for each print stacking orientation
are
summarized in Table 3. These theoretical results, along with the structural anisotropy introduced
by additive manufacturing, explain the experimental behavior of the oriented parts shown in
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Figure 6 and Figure 7, and summarized in Table 2. The parts manufactured in the 0 and 45
orientations had the lowest compressive strength, 13.50 1.95 MPa and 13.43 4.60 MPa,
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respectively. This occurs because the layer orientations within those parts coincide with the
direction perpendicular to the principal normal stress for
principal shear stress for
stress tensor theory, does not experience shear or normal stress, therefore the loading is
distributed along the parallel stacked build planes, where the inter-particle contact results in the
strongest sinter neck formation, therefore these parts show the highest compression strength of
45.13 6.82 MPa. When comparing the compressive strength of the 30 orientation, 20.60
6.23 MPa, and 60 orientation, 28.19 2.46 MPa, it can be seen that the 30 orientation can
sustain a lower compressive strength, as the stacked build planes along the 30 orientation
experienced a higher normal stress than compared to the 60 orientation. The experimental
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results, supported by the stress tensor theory and the structural anisotropy hypothesis,
demonstrate that the 3DP process can significantly influence the mechanical strength of parts.
30
45
90
120
135
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90
150
180
Normal stress to
orientation
60
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Print orientation ()
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Table 3 Normal and shear stress distribution relative to layering planes for samples manufactured
with stacked layers of orientation 0, 30, 45, 60, 90 deg respectively
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In this study, there is an inversely proportional correlation between the measured porosity and
compressive strength, where the lowest porosity of 30% corresponded to the highest mechanical
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strength of 45 MPa, and the highest porosity of 38% and 37% corresponded to the lowest
mechanical strength of 13.5 MPa and 13.4 MPa respectively. This finding is in accordance
with the literature, where an increased porosity is ideal for promoting bone ingrowth into bone
substitutes, however this comes at the cost of reducing mechanical properties [33,36,37]. The
reason for the observed volume % porosity difference between sample categories is not clear but
may be related to the level of binder deposited per volume of sample during the powder layer
build-up.
The porosity of all samples is between the measured porosity of trabecular bone (50-90%) and
cortical bone (3-12%) [33]. In addition, the compressive strength of each category of samples is
between the measured ultimate strength of trabecular bone (4-12 MPa) and cortical bone (130180 MPa) [7]. From a previous study [27], the pore size for CPP samples appears independent of
layer orientation, and ranges between 20-150 m in size, with a mean of ~56 m, which is in an
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acceptable range for bone substitutes [33,38,39]. The range of compressive strength obtained in
this study for CPP samples at 90 orientation (45MPa) is similar in value with values obtained
in other studies of 3D printing of CPP at the same orientation (50 MPa) [27]. Furthermore, the
reported for the CPP samples fabricated in this work (3-12) are
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comparable with reported Weibull moduli reported for calcium phosphate samples in the
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literature (3-9)[40] and (5-10)[27], and indicate good reliability in measurements presented in
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this work, as the Weibull modulus is inversely proportional with data scatter. A higher
correlates with a good distribution of porous distribution within the part and good repeatability
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[41].
Based on the results shown in this study, the powder-based additive manufacturing process using
CPP as the raw material is a promising approach in manufacturing implants as bone substitutes
or as components of biphasic tissue-engineered constructs for osteochondral defect repair.
Furthermore, the AM-made structures are anisotropic in nature, offering the possibility of
aligning the layer stacking orientation during part fabrication perpendicular to the path of
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maximum anticipated compressive loading based on kinetic and kinematic data. This benefit will
be explored in a future work. The layer orientation within the part is an important design
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parameter in manufacturing bone substitutes, as the loading kinetics and kinematics on the part
can be estimated and layer orientation can be tuned to ensure greater probability of implant
survival under peak loading conditions.
Conclusion
In this work, the powder-based additive manufacturing process was studied to quantify the effect
of powder layer orientation on mechanical properties of the generated parts. It has been shown
that the layer orientation within the part has a significant influence on the compressive strength
of the resulting structure. Thus layer orientation is an important optimization parameter in the
additive manufacturing design cycle. Furthermore, the results shown in this study can be used to
tune the mechanical strength of an implant along the orientation of maximum loading, if this is
known during the implant design stage. It was concluded that samples made with the 90
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stacking orientation have the highest compressive strength (45.13 6.82 MPa), whereas those
made with the 0 and 45 stacking orientations exhibit the weakest compressive strength (13.50
1.95 MPa).
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Acknowledgment
The authors appreciate the funding support received from The Natural Sciences and Engineering
References
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Szpalski C, Wetterau M, Barr J, Warren SM. Bone tissue engineering: current strategies
and techniques Part I: Scaffolds. Tissue Eng Part B-Reviews 2012;18:24657.
doi:10.1089/ten.teb.2011.0427.
[2]
[3]
Koh JL. The effect of graft height mismatch on contact pressure following osteochondral
grafting: a biomechanical study. Am J Sports Med 2004;32:31720.
doi:10.1177/0363546503261730.
[4]
[5]
Frost HM. Bones mechanostat: a 2003 update. Anat Rec A Discov Mol Cell Evol Biol
2003;275:1081101. doi:10.1002/ar.a.10119.
[6]
Leong K, Cheah CM, Chua CK. Solid freeform fabrication of three-dimensional scaffolds
for engineering replacement tissues and organs. Biomaterials 2003;24:236378.
doi:10.1016/S0142-9612(03)00030-9.
[7]
Yang S, Leong KF, Du Z, Chua CK. The design of scaffolds for use in tissue engineering.
Part I. Traditional factors. Tissue Eng 2001;7:67989. doi:10.1089/107632701753337645.
[8]
Yang S, Leong K-F, Du Z, Chua C-K. The design of scaffolds for use in tissue
engineering. Part II. Rapid prototyping techniques. Tissue Eng 2002;8:111.
doi:10.1089/107632702753503009.
Ac
ce
p
te
[1]
19
Page 19 of 22
Yeong W-Y, Chua C-K, Leong K-F, Chandrasekaran M, Lee M-W. Indirect fabrication of
collagen scaffold based on inkjet printing technique. Rapid Prototyp J 2006;12:22937.
doi:10.1108/13552540610682741.
[10]
[11]
Shanjani Y, De Croos JNA, Pilliar RM, Kandel RA, Toyserkani E. Solid freeform
fabrication and characterization of porous calcium polyphosphate structures for tissue
engineering purposes. J Biomed Mater Res B Appl Biomater 2010;93:5109.
doi:10.1002/jbm.b.31610.
[12]
[13]
Vorndran E, Klarner M, Klammert U, Grover LM, Patel S, Barralet JE, et al. 3D Powder
Printing of -Tricalcium Phosphate Ceramics Using Different Strategies. Adv Eng Mater
2008;10:B6771. doi:10.1002/adem.200800179.
[14]
Lu K, Reynolds WT. 3DP process for fine mesh structure printing. Powder Technol
2008;187:118. doi:10.1016/j.powtec.2007.12.017.
[15]
Suwanprateeb J. Influence of printing parameters on the transformation efficiency of 3Dprinted plaster of paris to hydroxyapatite and its properties. Rapid Prototyp J
2012;18:4909. doi:10.1108/13552541211272036.
[16]
Hsu TJ, Lai WH. Manufacturing parts optimization in the three-dimensional printing
process by the taguchi method. J Chinese Inst Eng 2010;33:12130.
[17]
[18]
[19]
[20]
Ac
ce
p
te
an
us
cr
ip
t
[9]
20
Page 20 of 22
Gbureck U, Hlzel T, Doillon CJ, Mller FA, Barralet JE. Direct printing of bioceramic
implants with spatially localized angiogenic factors. Adv Mater 2007;19:795800.
doi:10.1002/adma.200601370.
[22]
Habibovic P, Gbureck U, Doillon CJ, Bassett DC, Blitterswijk CA, Barralet JE.
Osteoconduction and osteoinduction of low-temperature 3D printed bioceramic implants.
Biomaterials 2008;29:94453. doi:10.1016/j.biomaterials.2007.10.023.
[23]
[24]
[25]
[26]
[27]
Shanjani Y, Hu Y, Pilliar RM, Toyserkani E. Mechanical characteristics of solidfreeform-fabricated porous calcium polyphosphate structures with oriented stacked layers.
Acta Biomater 2011;7:178896. doi:10.1016/j.actbio.2010.12.017.
[28]
Pilliar RM, Filiaggi MJ, Wells JD, Grynpas MD, Kandel RA. Porous calcium
polyphosphate scaffolds for bone substitute applications - in vitro characterization.
Biomaterials 2001;22:96372.
[29]
Pilliar RM, Kandel R a, Grynpas MD, Hu Y. Porous calcium polyphosphate as loadbearing bone substitutes: in vivo study. J Biomed Mater Res B Appl Biomater
2013;101:18. doi:10.1002/jbm.b.32832.
[30]
Ac
ce
p
te
an
us
cr
ip
t
[21]
21
Page 21 of 22
Pilliar RM, Hong J, Santerre PJ. Method of manufacture of porous inorganic structures,
Patent Number 7494614. 7494614, 2009.
[32]
[33]
[34]
[35]
[36]
[37]
Lin ASP, Barrows TH, Cartmell SH, Guldberg RE. Microarchitectural and mechanical
characterization of oriented porous polymer scaffolds. Biomaterials 2003;24:4819.
[38]
Porter JR, Ruckh TT, Popat KC. Bone tissue engineering: a review in bone biomimetics
and drug delivery strategies. Biotechnol Prog 2009;25:153960. doi:10.1002/btpr.246.
[39]
[40]
[41]
Ac
ce
p
te
an
us
cr
ip
t
[31]
22
Page 22 of 22