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SR0183,BoltonBrothSelectiveSupplement|OxoidProductDetail
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Campylobacter

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CultureMediaSupplements
BOLTONSELECTIVEENRICHMENTBROTH
BOLTONBROTH
CODE:CM0983
AmediumfortheselectivepreenrichmentofCampylobacterorganismsinfoodsamples.
TypicalFormula*

gm/litre

Meatpeptone

10.0

Lactalbuminhydrolysate

5.0

YeastExtract

5.0

ForthisOrganism

Sodiumchloride

5.0

Otherproductsusedinthe
isolationofCampylobacter:
AG0025
AnaeroJar

Alphaketoglutaricacid

1.0

Sodiumpyruvate

0.5

Sodiummetabisulphite

0.5

CN0020
CampyGenCompact
CN0025
CampyGen2.5litre
CN0035
CampyGen3.5litre
CM0271
BloodAgarBaseNo2
CM0689
CampylobacterAgarBase
CM0739
BloodFreeCampylobacter
SelectiveAgarBase
CM0935
CampylobacterAgarBase
(Karmali)
CM0983
BoltonBroth
SR0048
LakedHorseBlood
SR0069
CampylobacterSelective
Supplement(Skirrow)
SR0085
CampylobacterSelective
Supplement(Butzler)
SR0098
CampylobacterSelective
Supplement(BlaserWang)
SR0117
PrestonCampylobacter
SelectiveSupplement
SR0155
CCDASelective
Supplement.
SR0167
CampylobacterSelective
Supplement(Karmali)
SR0174
C.A.T.Supplement

Business
Continuity

Sodiumcarbonate

0.6

Haemin

0.01

pH7.40.2@25C

*Adjustedasrequiredtomeetperformancestandards

BOLTONBROTHSELECTIVESUPPLEMENT
CODE:SR0183
Vialcontents(eachvialissufficientfor500mlofmedium)

pervial

perlitre

Cefoperazone

10.0mg

20.0mg

Vancomycin

10.0mg

20.0mg

Trimethoprim.

10.0mg

20.0mg

Cycloheximide

25.0mg

50.0mg

Directions
Add13.8gofBoltonBrothto500mlofdistilledwater.Sterilisebyautoclavingat121Cfor15minutes.
Coolto50C.Asepticallyadd25mlLakedHorseBloodSR0048and1vialofBoltonBrothSelective
SupplementSR0183,reconstitutedasdirected.Mixwellanddistributeintosterilescrewtopcontainers.
Description
BoltonSelectiveEnrichmentBrothisintendedforthepreenrichmentofCampylobacterinfoodsamples.
CampylobacterareGramnegative,spirallyshapedmicroaerophilicorganismswhichmaybepresentin
rawmilk,untreatedwater,improperlyhandledfoodandundercookedmeats,poultryandshellfish.
Humanconsumptionoftheseorganismscanresultinarangeofclinicalillnessesfromtransient
asymptomaticcolonisationtoseveredysentery.ThesymptomsofCampylobacterenteritisinclude
diarrhoea,stomachpain,nausea,fever,headacheandmusclepain.Complicationsofinfectionby
Campylobacterjejunimayincludeunnecessaryappendectomiesasaresultofabdominalpain,reactive
arthritisorGuillianBarrsyndrome1.Campylobacterinfectionisrecognisedasoneofthemostcommon
causesofbacterialgastroenteritisinhumans,andtheminimuminfectivedosemaybeaslowas500800
cells1.
SinceawarenessoftheapparentroleofCampylobacterinhumandiseasewasheightenedbySkirrowin
19772,agreatnumberofculturemediahaveevolvedinresponsetotheneedtooptimiseperformance.
Therewasearlyrecognitionoftheneedforenrichmentculturewhenexaminingfoodsamplesto
overcomethedamagingeffectsthatfoodprocessingandpreservationtechniquescanhaveon
Campylobactercells.Useoflowerincubationtemperaturesintheearlystagesofenrichmentisnow
widelyestablishedasanaidtocellrecovery3.ThisprinciplewasemployedbyBoltoninthedevelopment
ofhisenrichmentbroth4.
Campylobactercanbeinjuredbyfoodprocessingandpreservationprocedures3.Thismakesthem
susceptibletoselectiveagentswhicharetoleratedbyundamagedcells.Falsenegativeresultsare
avoidedthroughuseofrecoverymediumsuchasBoltonSelectiveEnrichmentBrothwhichincreasesthe
numberofcellsavailableforculture,firstbyresuscitatinginjuredorganismsandthenencouragingthem

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SR0183,BoltonBrothSelectiveSupplement|OxoidProductDetail
SR0183
BoltonBrothSelective
Supplement

allgenius

SR0204
ModifiedPreston
CampylobacterSelective
Supplement

RelatedSearches

SR0205
ModifiedKarmaliSelective
Supplement
SR0208
ModifiedBoltonBroth
SelectiveSupplement
?

DrugDevelopment
ThermoFisher
ChromatographyAnd

SR0232
CampylobacterGrowth
Supplement(Liquid)
LT0504
FoodbornePathogens,
MonographNumber3
Campylobacter

MassSpectrometry

DR0150
CampylobacterTestKit

Pharmaceutical

QB0003
DuPontQUALICONBAX
SYSTEMQ7

Research

QB3449
BAXCAMPYLOBACTER
JEJUNI/COLI/LARIKIT(96
tests)
PB0118
CAMPYLOBACTER
SELECTIVEAGAR
(SKIRROW)
PB0295
CAMPYLOBACTER
SELECTIVEAGAR
(PRESTON)
PO0839
CAMPYLOBACTERCAT
AGAR
PO1185
BrillianceCampyCount
AGAR

tomultiply.
BoltonSelectiveEnrichmentBrothcontainsnutrientstoaidresuscitationofsublethallyinjuredcells,and
isformulatedtoavoidtheneedforamicroaerobicatmosphere.Initialincubationiscarriedoutat30
37C,dependingonthetypeoffoodtobeexamined.Afterthepreenrichment,theincubation
temperatureisraisedto42Ctoincreasetheselectivepressuresoncompetingorganisms.
InclusionofsodiummetabisulphiteandsodiumpyruvateinBoltonBrothquenchestoxiccompoundsthat
mayformintheculturemedium.Theseadditionsalsoincreasetheaerotoleranceoftheculture.The
antibioticscontainedinBoltonBrothSelectiveSupplementSR0183optimiseselectivityfor
Campylobacterspp.VancomycinactiveagainstGrampositives.Cefoperazonepredominantlyactive
againstGramnegatives.TrimethoprimactiveagainstawidevarietyofGramnegativeandGram
positiveorganisms.Cycloheximideactiveagainstyeasts.
Technique
Onemethodofuseisasfollows:
Place25goffoodsamplein225mlBoltonSelectiveEnrichmentBroth(preparedasdescribedabove)
andhomogenisethemixtureusingaStomacher(orsimilardevice).BoltonSelectiveEnrichmentBroth
doesnotrequireincubationinamicroaerobicenvironment,butmustbeusedinscrewtoppedcontainers
whicharefilledtowithin20mmofthetop4.Incubatefor4hoursat37C,followedbyfurtherincubation
at42C.Thebrothcanbesubculturedafter24hoursand48hoursontoeitherModifiedCCDA
(CM0739+SR0155)orPrestonAgar(CM0689+SR0117+SR0048)4.
ForothermethodspleaserefertoBAM5.
StorageconditionsandShelfLife
Storethedehydratedmediumat1030Candusebeforetheexpirydateonthelabel.
Storethepreparedmediumat28Cforupto2weeks.
Appearance
Dehydratedmedium:Strawcoloured,freeflowingpowder
Preparedmedium:Broth:Strawcolouredsolutioncontainingsmallblackparticles
Qualitycontrol
Positivecontrol:

Expectedresults:whensubculturedonmodifiedCCDA

CampylobacterjejuniATCC29428*

Goodgrowthgreycolouredcolonies

Negativecontrol:

EscherichiacoliATCC25922*

Nogrowth

*ThisorganismisavailableasaCultiLoop.

References
1.NationalAdvisoryCommitteeonMicrobiologicalCriteriaforFoods(1994).JournalofFoodProtection
57(12):11011121.
2.Skirrow,M.B.(1977).BritishMedicalJournal2:911.
3.Post,D.E.(1995).FoodBornePathogensMonographNumber3Campylobacter.
4.Bolton,F.J.(1995)Personalcommunication.
5.Hunt,J.M.(1998)Campylobacter.In:F.D.A.BacteriologicalAnalyticalManual,8thEdition(Revision
A)7.017.27.AOAC,ArlingtonVa.

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