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Product Information

PrimeQ KSOM Mouse Embryo Culture Medium (1X) w/ AA and Phenol


Mouse embryo culture medium. Sterile-filtered.
Catalog No. GSM-5140
Unit Size
50 mL / 1X
Shipping
Frozen on dry ice
Storage
Store frozen at -20C to -80 C
Do not use a frost-free freezer. The product should be used immediately after
thawing, but can be stored at 2-8C for 1 week.
Shelf Life
1.5 Year at -20C to -80 (frozen); 1 week at 4C (thawed)
Mouse embryo assay (MEA ) for assessing toxicity and ability of the media to support
Quality
the development of one- and two-cell mouse embryos to expanded blastocysts;
Testing
Independently tested by Charles River Laboratories

Safety
Information

BioSafety Level: 1
Safety conditions pertaining to well characterized agents not known to cause disease
in healthy adult humans and of minimal potential hazard to laboratory personnel and
the environment should be used. Lab coats and gloves should be worn at all times.
Face protection is recommended when thawing a frozen vial stored in a liquid nitrogen
freezer.

Intended Use
KSOM mouse embryo medium is commonly used for in-vitro mouse embryo culturing KSOM and has
been shown to support the development of mouse preimplantation embryos in culture. Additional
studies have also shown that KSOM supplemented with amino acids increases the rate of blastocyst
stage development.1,2,3 For Research Use Only (RUO). Caution: Not intended for human or animal
diagnostic or therapeutic uses.
Directions for Use
Thaw frozen bottle of medium in a refrigerator (2C to 8C) overnight or slowly at room temperature.
Gently swirl thawed medium to mix and warm to 37C prior to use. Perform all actions using aseptic
culture conditions.
Once thawed, the medium can be stored for 1 week at 2C to 8C. Do not use past expiration date.
Medium used past the recommended expiration date may not perform optimally.
Note: The pH of sodium bicarbonate (NaHCO3) only buffered formulations will rise upon exposure to air
and should be adjusted as needed prior to use.
Equivalent to Millipore Cat. No. MR-021-D
References:
1JA Lawitts and JD Biggers, (1993) Culture of preimplantation embryos. Methods Enzymol., 225, 153-164.
2GT Erbach, et al., (1994) Differential growth of the mouse preimplantation embryo in chemically defined media. Biol. Reprod.,
50, 10271033.
3Y Ho, et al., (1995) Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis

of gene expression. Mol. Reprod. Dev., 41, 232238


CONTACT / ORDERING INFORMATION
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