Professional Documents
Culture Documents
to accompany
Terry R. Martin
Kishwaukee College
HUMAN ANATOMY AND PHYSIOLOGY LABORATORY MANUAL, FETAL PIG DISSECTION, THIRD EDITION.
Published by McGraw-Hill Higher Education, an imprint of The McGraw-Hill Companies, Inc.,
1221 Avenue of the Americas, New York, NY 10020. Copyright 2007 by The McGraw-Hill
Companies, Inc. All rights reserved.
The contents, or parts thereof, may be reproduced in print form solely for classroom use with
HUMAN ANATOMY AND PHYSIOLOGY LABORATORY MANUAL, FETAL PIG DISSECTION, SECOND EDITION BY TERRY R.
MARTIN, provided such reproductions bear copyright notice, but may not be reproduced in any other form or for any other purpose without the
prior written consent of The McGraw-Hill Companies, Inc., including, but not limited to, in any network or other electronic storage or
transmission, or broadcast for distance learning.
Acid-free paper
1 2 3 4 5 6 7 8 9 0 QPD/QPD 0 3 2 1 0
ISBN 0-07-282734-3
www.mhhe.com
ii
CONTENTS
PREFACE ....................................................................................................................................................................................................vi
AN OVERVIEW .......................................................................................................................................................................................... vii
INSTRUCTIONAL APPROACHES........................................................................................................................................................... viii
CORRELATION OF TEXTBOOK SYSTEMS AND SUPPLEMENTAL FROG LABORATORY EXERCISES.........................................ix
SUGGESTED TIME SCHEDULE ............................................................................................................................................................... x
Cells
Laboratory Exercise 5 Cell Structure and Function ................................................................................................................................ 6
Laboratory Exercise 6 Movements Through Cell Membranes ............................................................................................................... 7
Laboratory Exercise 7 Cell Cycle ............................................................................................................................................................ 8
Tissues
Laboratory Exercise 8 Epithelial Tissues ................................................................................................................................................ 9
Laboratory Exercise 9 Connective Tissues ........................................................................................................................................... 10
Laboratory Exercise 10 Muscle and Nervous Tissues ......................................................................................................................... 11
Integumentary System
Laboratory Exercise 11 Integumentary System .................................................................................................................................... 12
Skeletal System
Laboratory Exercise 12 Bone Structure and Classification .................................................................................................................. 13
Laboratory Exercise 13 Organization of the Skeleton .......................................................................................................................... 14
Laboratory Exercise 14 Skull ................................................................................................................................................................. 15
Laboratory Exercise 15 Vertebral Column and Thoracic Cage ............................................................................................................ 17
Laboratory Exercise 16 Pectoral Girdle and Upper Limb ..................................................................................................................... 19
Laboratory Exercise 17 Pelvic Girdle and Lower Limb ......................................................................................................................... 21
Laboratory Exercise 18 Joints ............................................................................................................................................................... 23
Muscular System
Laboratory Exercise 19 Skeletal Muscle Structure ............................................................................................................................... 24
Laboratory Exercise 20 Muscles of the Face, Head, and Neck ........................................................................................................... 25
Laboratory Exercise 21 Muscles of the Chest, Shoulder, and Upper Limb ......................................................................................... 26
Laboratory Exercise 22 Muscles of the Deep Back, Abdominal Wall, and Pelvic Outlet .................................................................... 27
Laboratory Exercise 23 Muscles of the Hip and Lower Limb .............................................................................................................. 28
Laboratory Exercise 24 Fetal Pig Dissection: Musculature .................................................................................................................. 29
Nervous System
Laboratory Exercise 25 Nervous Tissue and Nerves ........................................................................................................................... 30
Laboratory Exercise 26 Meninges and Spinal Cord ............................................................................................................................. 31
Laboratory Exercise 27 Reflex Arc and Reflexes ................................................................................................................................. 32
Laboratory Exercise 28 Brain and Cranial Nerves................................................................................................................................ 33
Laboratory Exercise 29 Dissection of the Sheep Brain ........................................................................................................................ 35
iii
Endocrine System
Laboratory Exercise 36 Endocrine Histology and Diabetic Physiology................................................................................................ 42
Cardiovascular System
Laboratory Exercise 37 Blood Cells ...................................................................................................................................................... 43
Laboratory Exercise 38 Blood Testing A Demonstration ................................................................................................................... 44
Laboratory Exercise 39 Blood Typing ................................................................................................................................................... 45
Laboratory Exercise 40 Heart Structure ................................................................................................................................................ 46
Laboratory Exercise 41 Cardiac Cycle .................................................................................................................................................. 48
Laboratory Exercise 42 Blood Vessels.................................................................................................................................................. 49
Laboratory Exercise 43 Pulse Rate and Blood Pressure ..................................................................................................................... 50
Laboratory Exercise 44 Arteries and Veins ........................................................................................................................................... 51
Laboratory Exercise 45 Fetal Pig Dissection: Cardiovascular System ................................................................................................ 53
Lymphatic System
Laboratory Exercise 46 Lymphatic System ........................................................................................................................................... 54
Digestive System
Laboratory Exercise 47 Digestive Organs............................................................................................................................................. 55
Laboratory Exercise 48 Fetal Pig Dissection: Digestive System.......................................................................................................... 57
Laboratory Exercise 49 Action of a Digestive Enzyme ......................................................................................................................... 58
Respiratory System
Laboratory Exercise 50 Respiratory Organs ......................................................................................................................................... 59
Laboratory Exercise 51 Fetal Pig Dissection: Respiratory System ...................................................................................................... 60
Laboratory Exercise 52 Breathing and Respiratory Volumes and Capacities ..................................................................................... 61
Laboratory Exercise 53 Control of Breathing ........................................................................................................................................ 62
Urinary System
Laboratory Exercise 54 Kidney Structure.............................................................................................................................................. 63
Laboratory Exercise 55 Urinalysis ......................................................................................................................................................... 64
Laboratory Exercise 56 Fetal Pig Dissection: Urinary System ............................................................................................................. 65
iv
PREFACE
This instructors manual is designed to assist those who are using the Human Anatomy and Physiology Laboratory Manual, Fetal
Pig Dissection, third edition by Terry R. Martin. It describes the purpose of the laboratory manual and its special features, and
provides suggestions for presenting the laboratory exercises to students. The instructors manual also parallels the laboratory
manual, exercise by exercise, providing labels for unlabeled diagrams and answers to questions that appear in the laboratory
reports. For some exercises, special instructional suggestions that propose alternative procedures, laboratory equipment, or
laboratory techniques are provided.
vi
AN OVERVIEW
The Human Anatomy and Physiology Laboratory Manual, Fetal Pig Dissection, third edition, was written to accompany any
human anatomy and physiology textbook. The laboratory manual is planned for students pursuing careers in allied health fields
who have minimal backgrounds in the physical and biological sciences.
The manual contains sixty-four laboratory exercises that are closely integrated with the content of a textbook (three of
these exercises only appear online at www.mhhe.com/shier11.) The exercises are designed to review and illustrate various
anatomical and physiological facts and principles presented in the textbook and to help students investigate some of these ideas in
more detail.
The laboratory exercises include a variety of special features that are designed to stimulate student interest in the
subject matter, to involve students in the learning process, and to guide them through the planned experiences. These features
include the following:
Materials Needed. The laboratory materials listed are those that students require to complete the exercise and to perform the
demonstrations and learning extensions.
Safety. If the laboratory exercise requires special safety guidelines, this section is included. General safety guidelines also appear
inside the front cover.
Some institutions might have committees for reviewing labs using live animals, biohazards, and physiology
experiments performed on students. Be sure your labs conform to their guidelines. You might find the forms available in
Appendixes 3 and 4 useful at your school.
Introduction. The introduction briefly describes the subject of the exercise or the ideas that will be investigated.
Purpose of the Exercise. The purpose provides a statement concerning the intent of the exercisethat is, what will be
accomplished.
Learning Objectives. The learning objectives list in general terms what a student should be able to do after completing the
exercise.
Procedure. The procedure provides a set of detailed instructions for accomplishing the planned laboratory activities. Usually
these instructions are presented in outline form so that a student can proceed through the exercise in stepwise fashion. Frequently,
the student is referred to particular sections of a textbook for necessary background information or for review of subject matter
presented in some previous part of the course.
The procedures include a wide variety of laboratory activities and, from time to time, direct the student to complete
various tasks in the laboratory reports.
Demonstrations. Demonstrations appear in separate boxes. They describe specimens, specialized laboratory equipment, or other
materials of interest that the instructor may want to display to enrich the students laboratory experience.
Learning Extensions. Learning extensions also appear in separate boxes. They are planned to encourage students to extend their
laboratory experiences. Some of these activities are open-ended in that they suggest how a student can plan an investigation or
experiment and carry it out after receiving approval from the laboratory instructor.
Illustrations. Diagrams are used as aids for reviewing subject matter. Other illustrations provide visual instructions for
performing steps in procedures or are used to identify parts of instruments or specimens. Micrographs often are included to help
students identify microscopic structures or to evaluate student understanding of tissues.
Some figures, such as those involving the skull, are presented so that they are suitable for coloring. You may want to
have your students use colored pencils to highlight various parts of these illustrations. This activity should enhance their ability to
observe the figures more carefully and help them locate and identify important anatomical features.
Laboratory Reports. Immediately following each exercise, there is a laboratory report to be completed by the student. These
reports include various types of review activities, spaces for sketches of microscopic objects, tables for recording observations
and experimental results, and questions dealing with the analysis of such data.
As a result of these laboratory exercises, students should develop a better understanding of the structural and functional
characteristics of their bodies. In addition, their skills in gathering information by observation and experimentation should
increase.
vii
INSTRUCTIONAL APPROACHES
Exercise Selection
Although the laboratory manual contains sixty-four separate exercises, it may not be possible to include all of them in any one
program. However, because many of the exercises are relatively short and because the procedures of others are divided into
sections, an instructor can easily select those exercises or parts of exercises that best meet the needs of a particular class.
These exercises also vary in the quantities of equipment needed to complete them; if necessary, an instructor can make
some selection based upon the amount of laboratory equipment available for use by a class.
Animal Dissection
In the laboratory manual, the fetal pig is used as the major animal to be dissected. Detailed instructions for dissecting certain
organs, such as the sheep brain, sheep heart, pig kidney, and mammalian eye are also included.
A laboratory option is to obtain a cadaver as a demonstration specimen. If this is not possible, consider a field trip to a
location that has a prosected cadaver. A minimum of two viewings is recommendedone during muscle study and the other near
the end of the course.
The Use of Animals in Biology Education*
The National Association of Biology Teachers (NABT) believes that the study of organisms, including nonhuman animals, is
essential to the understanding of life on Earth. NABT recommends the prudent and responsible use of animals in the life science
classroom. NABT believes that biology teachers should foster a respect for life. Biology teachers also should teach about the
interrelationship and interdependency of all things.
Classroom experiences that involve nonhuman animals range from observation to dissection. NABT supports these
experiences so long as they are conducted within the long-established guidelines of proper care and use of animals, as developed
by the scientific and educational community.
As with any instructional activity, the use of nonhuman animals in the biology classroom must have sound educational
objectives. Any use of animals, whether for observation or dissection, must convey substantive knowledge of biology. NABT
believes that biology teachers are in the best position to make this determination for their students.
NABT acknowledges that no alternative can substitute for the actual experience of dissection or other use of animals
and urges teachers to be aware of the limitations of alternatives. When the teacher determines that the most effective means to
meet the objectives of the class do not require dissection, NABT accepts the use of alternatives to dissection including models
and the various forms of multimedia. The Association encourages teachers to be sensitive to substantive student objections to
dissection and to consider providing appropriate lessons for those students where necessary.
To implement this policy, NABT endorses and adopts the Principle and Guidelines for the use of Animals in
Precollege Education of the Institute of Laboratory Animals Resources (National Research Council). Copies of the Principle
and Guidelines may be obtained from the ILAR (2101 Constitution Avenue, NW, Washington DC 20418; 202-334-2590).
*Adopted by the Board of Directors in October 1995. This policy supersedes and replaces all previous NABT statements
regarding animals in biology education.
Background Information
The procedures of many exercises begin by suggesting that students review specific sections of the textbook. If the subject matter
involved in a particular exercise has been covered recently in lecture, the students may be able to accomplish such a review rather
quickly. On the other hand, if the material has not been presented previously, this part of a procedure may be used as a means of
introducing information needed to understand the ideas presented in the exercise.
When the procedure is used to introduce new material, an instructor may ask students to complete the first section before coming
to the laboratory. Following this, some portion of the laboratory time may be needed for class discussion of the new material.
viii
Muscular System
Ex. 62
Nervous System
Ex. 63
Cardiovascular System
Ex. 64
ix
Laboratory Exercise
Minimal Time
Ex. 33 Equilibrium
1 hr.
Ex. 34 Eye Structure
3 hr.
Ex. 35 Visual Tests and Demonstrations
2 hr.
Ex. 36 Endocrine Histology and Diabetic Physiology 2 hr.
Ex. 37 Blood Cells
2 hr.
Ex. 38 Blood TestingA Demonstration
2 hr.
Ex. 39 Blood Typing
1 hr.
Ex. 40 Heart Structure
2 hr.
Ex. 41 Cardiac Cycle
3 hr.
Ex. 42 Blood Vessels
1 hr.
Ex. 43 Pulse Rate and Blood Pressure
2 hr.
Ex. 44 Arteries and Veins
2 hr.
Ex. 45 Fetal Pig Dissection: Cardiovascular System 3 hr.
Ex. 46 Lymphatic System
1 hr.
Ex. 47 Digestive Organs
2 hr.
Ex. 48 Fetal Pig Dissection: Digestive System
2 hr.
Ex. 49 Action of a Digestive Enzyme
2 hr.
Ex. 50 Respiratory Organs
2 hr.
Ex. 51 Fetal Pig Dissection: Respiratory System
2 hr.
Ex. 52 Breathing and Respiratory Volumes and
Capacities
1 hr.
Ex. 53 Control of Breathing
1 hr.
Ex. 54 Kidney Structure
2 hr.
Ex. 55 Urinalysis
3 hr.
Ex. 56 Fetal Pig Dissection: Urinary System
1 hr.
Ex. 57 Male Reproductive System
2 hr.
Ex. 58 Female Reproductive System
2 hr.
Ex. 59 Fetal Pig Dissection: Reproductive Systems
2 hr.
Ex. 60 Fertilization and Early Development
2 hr.
Ex. 61 Genetics
2 hr.
Ex. 62 Skeletal Muscle Contraction
3 hr.
Ex. 63 Nerve Impulse Stimulation
3 hr.
Ex. 64 Factors Affecting the Cardiac Cycle
3 hr.
LABORATORY EXERCISE 1
SCIENTIFIC METHOD AND MEASUREMENTS
LABORATORY EXERCISE 2
BODY ORGANIZATION AND TERMINOLOGY
Instructional Suggestions
If a dissectible human torso model (manikin) is not available, you might want to have students consult the figures in various
sections of a textbook, particularly the body sections in the reference plates, to gain some understanding of the organizational
pattern of the human body.
Figure Labels
FIG. 2.1
1. Thoracic cavity
2. Abdominal cavity
3. Abdominopelvic cavity
4.
5.
6.
Pelvic cavity
Cranial cavity
Vertebral canal (spinal cavity)
FIG. 2.2.a
1. Visceral pleura
2. Pleural cavity
3. Parietal pleura
4.
5.
6.
FIG. 2.2b
1. Visceral peritoneum
2. Peritoneal cavity
3.
Parietal peritoneum
FIG. 2.5
1. Sagittal plane (midsagittal plane)
2. Coronal (frontal) plane
3.
FIG. 2.6a
1. Epigastric region
2. Right hypochondriac region
3. Right lumbar region
4. Umbilical region
5. Right iliac region (right inguinal region)
6.
7.
8.
9.
12.
13.
FIG. 2.6b
10. Right upper quadrant (RUQ)
11. Right lower quadrant (RLQ)
FIG. 2.7a
1. Nasal
2. Oral
3. Cervical
4. Acromial
5. Axillary
6. Mammary
7. Brachial
8.
9.
10.
11.
12.
13.
14.
Antecubital
Abdominal
Antebrachial
Carpal
Palmar
Digital
Gential
15.
16.
17.
18.
19.
20.
21.
Crural
Tarsal
Cephalic
Frontal
Orbital
Buccal
Mental
22.
23.
24.
25.
26.
27.
28.
FIG. 2.7b
29. Otic
30. Occipital
31. Acromial
32. Vertebral
33.
34.
35.
36.
Brachial
Dorsum
Cubital
Lumbar
37.
38.
39.
40.
Sacral
Gluteal
Perineal
Femoral
41. Popliteal
42. Sural (calf) or crural (leg)
43. Plantar
Sternal
Pectoral
Umbilical
Inguinal
Coxal
Patellar
Pedal
4.
5.
6.
a
b
c
7.
8.
9.
a
c
d
10. e
11. d
12. a
PART B
1. c
2. d
3. h
4.
5.
6.
g
j
i
7.
8.
9.
e
f
k
10. b
11. a
PART C
1. Inferior
2. (Correct)
3. (Correct)
4.
5.
6.
Anterior
(Correct)
(Correct)
7.
8.
9.
Distal
(Correct)
Superficial
10. (Correct)
11. Contralateral
12. Deep
PART D
1. e
2. k
3. b
4.
5.
6.
i
l
j
7.
8.
9.
g
c
d
10. h
11. f
12. a
PART E
1. h
2. c
3. i
4.
5.
6.
j
l
f
7.
8.
9.
k
g
d
10. a
11. b
12. e
4.
5.
6.
RUQ
LUQ or LLQ
LUQ
LABORATORY EXERCISE 3
CHEMISTRY OF LIFE
Instructional Suggestions
1. The 7 assorted common liquids for the pH tests could include among the following: orange juice, lemon juice, milk, bottled
water, baking soda solution, aspirin solution, borax solution, ammonia, vinegar, antacid, liquid soap, cola, and diluted
catsup. Any beverage can be used, and any household product, even solids if they can be dissolved.
2.
The unknown compounds could include among the following: diluted milk, diluted corn syrup, chicken or beef broth,
diluted potato soup, diluted pudding, salad dressing, egg substitute, and diluted yogurt. It is very important to use some
numbering code to keep track of which unknown is which. The unknowns often look alike and are easily confused. The
students need to be instructed to take care not to contaminate their unknown. They need to be reminded to perform all tests,
as students often think if they get one positive result they can stop. Be aware that different brands of food products can
contain different ingredients. Inform the students when heating the samples using Benedicts solution, if there is a protein
present it may denature or become solid, which should not be interpreted as a positive result.
5.
6.
7.
8.
f
i
c
d
4.
Various substances
dissolved in tap water
will influence the pH
3.
4.
(experimental results)
(experimental results)
9.
10.
11.
12.
k
g
a
l
5.
6.
Ionic
(sketches)
5.
(experimental results)
LABORATORY EXERCISE 4
CARE AND USE OF THE MICROSCOPE
Instructional Suggestions
1. To stimulate student interest in use of the microscope, you may want to have students prepare wet mounts of pond water and
observe the various forms of life present. A plankton net is a helpful device to concentrate pond organisms. Students can be
encouraged to bring samples of pond water to class in preparation for this experiment.
2.
You may want to provide students with prepared slides of major human organs to examine as a way of increasing their
experience with using the microscope.
3.
If oil-immersion objectives are available, you may want to provide students with prepared slides of various forms of bacteria
to observe using these objectives.
2. 1,000
PART B
1. (sketch)
2. About 4.5 mm for scanning power (using
4 objective)
3. About 4,500 micrometers
PART C
1. (sketch)
2. About 1.7 mm (using a 10 objective)
3. The diameter of the scanning-power field of view is
about 2.6 times greater than that of the low-power
field of view.
4. Student is unable to see two adjacent mm lines on the
scale in a high-power field of view.
PART D
1. f
6. j
2. i
7. d
3. c
8. b
4. a
9. g
5. h
10. e
4.
5.
About 2.2 mm
About 2,200 micrometers
5.
6.
7.
8.
9.
PART E
(sketches)
LABORATORY EXERCISE 5
CELL STRUCTURE AND FUNCTION
Instructional Suggestions
1. Instead of preparing cheek cell slides, you may want to have students prepare slides of plant cells using Elodea leaves or
onion skin.
2. If live frogs are available, you may want to pith the frogs and have students prepare wet mounts using small samples of the
ciliated epithelium that lines the oral cavity. They also can prepare smears of frog blood and stain the cells with methylene
blue, and prepare wet mounts of sperm cells from the testes of the male frogs. You then might provide students with
prepared slides of human ciliated epithelium, blood, and sperm cells and have the students compare the frog cells with the
human cells.
Figure Labels
FIG. 5.1
1. Flagellum
2. Centrioles
3. Golgi apparatus
4. Smooth endoplasmic reticulum
5. Nucleus
6.
7.
8.
9.
10.
Nuclear envelope
Mitochondrion
Ribosomes
Cell membrane
Cilia
5.
6.
7.
8.
i
f
c
b
9.
10.
11.
12.
PART B
1. (sketch)
3.
2. The wet-mount cells look like shells or ghosts.
The stained cells made the nucleus and other cellular
components more clearly visible.
PART C
1. (sketches)
2. They should always notice cytoplasm, nucleus,
nuclear envelope, and cell membrane
3.
d
e
h
j
Yes. The stained cheek cells are essentially the same size
and shape; however, the process of cell removal may cause
many of the cells to become folded and distorted.
LABORATORY EXERCISE 6
MOVEMENTS THROUGH CELL MEMBRANES
Instructional Suggestion
Instead of using human blood for Procedure C, you may want to substitute some other type of animal blood obtained from a meat
packing house, a veterinarian, or a biological supplier. The hemolysis experiment, using RBCs from a safe source, demonstrates
concepts of osmosis, tonicity, and membrane characteristics.
Laboratory Report Answers
PART A
1. (experimental results)
2. (experimental results)
3. Answers will vary.
4.
4.
5.
No
Yes
4.
4.
5.
Yes
Yes
3.
4.
5.
6.
4.
5.
No
Yes
Yes
Yes
No
PART B
1. Answers will vary.
2. Answers will vary.
3. A greater volume of fluid is in the thistle tube.
Yes
Yes
No
PART C
1. (sketches)
2. Tube 3. There was a net movement of water out of
the cells.
PART D
1. Water, glucose, and starch.
2. The tests for glucose and starch were positive.
3. Gravity
4. Charcoal
No
Yes
No
LABORATORY EXERCISE 7
CELL CYCLE
Figure Labels
FIG. 7.2
1. Chromosome (chromatid)
2. Centromere
3.
4.
Centriole
Spindle fiber (microtubules)
PART B
(sketches)
PART C
1. Each new cell contains identical chromosomes.
2. They may be slightly different in size and numbers
of organelles.
3.
c.
d.
Prophase
Anaphase
4.
5.
6.
Nuclear envelope
Centrioles/centrosome
Spindle fibers (microtubules)
LABORATORY EXERCISE 8
EPITHELIAL TISSUES
Laboratory Report Answers
PART A
1. f
2. d
3. c
4.
5.
6.
d
c
d
7.
8.
9.
e
f
b
10. a
11. a
12. e
PART B
(sketches)
LABORATORY EXERCISE 9
CONNECTIVE TISSUES
Laboratory Report Answers
PART A
1. e
2. a
3. b
4. c
5.
6.
7.
8.
i
d
a
h
9.
10.
11.
12.
g
c
f
j
PART B
(sketches)
10
LABORATORY EXERCISE 10
MUSCLE AND NERVOUS TISSUES
Laboratory Report Answers
PART A
1. b
2. a
3. d
4. a
5.
6.
7.
8.
c
b
c
a
9. d
10. b
PART B
(sketches)
11
LABORATORY EXERCISE 11
INTEGUMENTARY SYSTEM
Figure Labels
FIG. 11.1
1. Epidermis
2. Dermis
3. Subcutaneous layer (hypodermis)
4. Hair shaft
5.
6.
7.
8.
Stratum corneum
Stratum basale
Sebaceous gland
Arrector pili muscle
9. Hair follicle
10. Sweat gland (eccrine gland)
11. Blood vessels
FIG. 11.2
1. Stratum corneum
2. Stratum lucidum
3.
4.
Stratum granulosum
Stratum spinosum
5.
6.
Stratum basale
Basement membrane
5.
6.
7.
8.
c
d
e
i
9.
10.
11.
12.
g
k
f
b
PART B
1. Answers will vary.
2. Answers will vary.
3. Epidermal cells at the base of the hair follicle
divide and grow, pushing older cells outward;
as these cells die, they become keratinized
parts of the hair.
4. The pigment melanin is produced by melanocytes.
PART C
1. Epidermis is the outer layer of the skin, whereas
dermis is the inner layer; the subcutaneous layer binds the
dermis to the underlying organs.
2. Cells of the stratum basale are living and reproduce actively;
cells of the stratum corneum are dead and keratinized
and form the surface layer of the skin.
PART D
1. Hair root
2. Dermis
3.
4.
3.
4.
PART E
(sketch)
12
LABORATORY EXERCISE 12
BONE STRUCTURE AND CLASSIFICATION
Figure Labels
FIG. 12.1
1. Articular cartilage (hyaline cartilage)
2. Spongy bone (red marrow)
3. Medullary cavity
4. Yellow marrow
5. Compact bone
FIG. 12.2
1. Spongy bone
2. Compact bone
3. Osteon
4. Periosteum
5. Central canal
6.
7.
8.
9.
Periosteum
Proximal epiphysis
Diaphysis
Distal epiphysis
6.
7.
8.
9.
10.
Perforating canal
Blood vessels
Nerve
Canaliculus
Osteocyte
4.
5.
6.
13
LABORATORY EXERCISE 13
ORGANIZATION OF THE SKELETON
Figure Labels
FIG. 3.1a
1. Cranial bones (cranium)5.
2. Facial bones (face)
6.
3. Skull
7.
4. Clavicle
Sternum
Rib
Vertebral column
(vertebra)
8.
9.
10.
11.
Coxa (hipbone)
Carpals
Metacarpal
Phalanx
12.
13.
14.
15.
FIG. 13.1b
1. Scapula
2. Humerus
3. Ulna
Radius
Femur
Tibia
7.
8.
Fibula
Vertebral column
(vertebra)
9. Sacrum
10. Coccyx
4.
5.
6.
Patella
Tarsals
Matatarsal
Phalanx
6.
7.
8.
9.
10.
Sternum
Twelve
Pectoral girdle
Ulna
Carpals
11.
12.
13.
14.
15.
Sacrum
Pelvis
Patella
Tarsals
Phalanges
PART B
1. c
2. f
3.
4.
a
e
5.
6.
g
b
7.
PART C
1. c
2. a
3.
4.
g
e
5.
6.
b
d
7.
4.
5.
6.
7.
Fibula
Ulna
Scapula
Radius
8.
9.
10.
11.
Clavicle
Humerus
Patella
Femur
12. Tibia
13. Rib
14
LABORATORY EXERCISE 14
SKULL
Instructional Suggestion
You might want to have the students use colored pencils to color the bones in figures 14.1 and 14.2. They should use a different
color for each of the individual bones in the series. This activity should cause the students to observe the figures more carefully
and help them to locate the various bones that are shown from different views in the figures. The students can check their work
by referring to the corresponding full-color figures in the textbook.
Figure Labels
FIG. 14.1
1. Parietal bone
2. Frontal bone
3. Coronal suture
4. Temporal bone
5. Perpendicular plate (of ethmoid bone)
6. Infraorbital foramen
7. Vomer bone
8. Mandible
9.
10.
11.
12.
13.
14.
15.
16.
Supraorbital foramen
Nasal bone
Sphenoid bone
Zygomatic bone
Middle nasal concha (of ethmoid bone)
Inferior nasal concha
Maxilla
Mental foramen
12.
13.
14.
15.
16.
17.
18.
19.
20.
Coronal suture
Frontal bone
Sphenoid bone
Lacrimal bone
Nasal bone
Zygomatic bone
Maxilla
Mandible
Coronoid process
FIG. 14.3
1. Maxilla
2. Zygomatic bone
3. Sphenoid bone
4. Vomer bone
5. Zygomatic arch
6. Styloid process
7. Mastoid process
8.
9.
10.
11.
12.
13.
14.
Occipital condyle
Temporal bone
Palatine process (of maxilla)
Palatine bone
Foramen magnum
Lambdoid suture
Occipital bone
FIG. 14.4
1. Ethmoid bone
2. Foramen magnum
3. Crista galli
4. Cribriform plate (olfactory foramina)
5. Frontal bone
6.
7.
8.
9.
10.
Sphenoid bone
Temporal bone
Sella turcica
Parietal bone
Occiptal bone
FIG. 14.5
1. Coronal suture
2. Frontal bone
3. Sphenoid bone
4. Frontal sinus
5. Nasal bone
6. Maxilla
7. Parietal bone
8. Temporal bone
9.
10.
11.
12.
13.
14.
15.
16.
Squamous suture
Lambdoid suture
Occipital bone
Sella turcica
Styloid process
Sphenoidal sinus
Vomer bone
Mandible
FIG. 14.2
1. Parietal bone
2. Squamous suture
3. Lambdoid suture
4. Temporal bone
5. Occipital bone
6. Temporal process (of zygomatic bone)
7. External acoustic meatus
8. Mastoid process
9. Styloid process
10. Mandibular condyle
11. Zygomatic process (of temporal bone)
15
4.
5.
6.
f
c
f
PART B
1. Coronal
2. Sagittal
3. Lambdoid
7.
8.
9.
f
a
c
10. e
11. f
12. b
4.
5.
6.
Squamous
Frontal, ethmoid, sphenoid
Maxilla
PART C
1. e
2. c
3. c
4.
5.
6.
h
d
g
7.
8.
9.
h
a
d
10. c
11. f
12. b
PART D
1. c
2. a
3.
4.
g
f
5.
6.
d
b
7.
6.
7.
8.
9.
Mandible
Middle nasal concha (of ethmoid bone)
Inferior nasal concha
Mental foramen
8.
9.
10.
11.
12.
13.
14.
Mandibular condyle
Coronal suture
Frontal bone
Zygomatic process (of temporal bone)
Zygomatic bone
Maxilla
Mandible
7.
8.
9.
10.
11.
5.
6.
7.
8.
Ethmoid bone
Sphenoid bone
Sella turcica
Foramen magnum
5.
6.
7.
Maxilla
Frontal bone
Mandible
16
LABORATORY EXERCISE 15
VERTEBRAL COLUMN AND THORACIC CAGE
Figure Labels
FIG. 15.1
1. Cervical vertebrae
2. Thoracic vertebrae
3. Lumbar vertebrae
4. Sacrum
FIG. 15.4
1. Sacral promontory
2. Anterior sacral foramen
3. Coccyx
4. Sacral canal
5.
6.
7.
Coccyx
Intervertebral foramina
Intervertebral discs
5.
6.
7.
8.
6.
7.
8.
9.
Xiphoid process
Sternum
Costal cartilage
Floating ribs
9.
10.
11.
12.
13.
14.
15.
Atlas
Dens
Lumbar
Five
Sacroiliac
Sacral promontory
Sacral hiatus
17
PART B
Vertebra
Number
Size
Body
Spinous Process
Cervical
Smallest
Smallest
Thoracic
12
Intermediate
Intermediate
Lumbar
Largest
Largest
C2 through C5 are
forked
Pointed and angled
downward
Short, blunt, and
nearly horizontal
PART C
1. 206
2. Floating
3. Transverse
4. Hyaline cartilage
PART D (FIG. 15.6)
1. Spinous process
2. Atlas
3. Axis
Transsverse
Foramina
Present
Absent
Absent
5.
6.
Clavicles
a. Supports shoulder girdle and upper limbs
b. Protects visceral organs
c. Functions in breathing
4.
5.
6.
Transverse process
Intervertebral disc
Body (of sixth cervical vertebra)
18
LABORATORY EXERCISE 16
PECTORAL GIRDLE AND UPPER LIMB
Figure Labels
FIG. 16.1
1. Clavicle
2. Rib
3. Sternum
4. Costal cartilage
5. Scapula
6. Humerus
7.
8.
9.
10.
11.
Ulna
Radius
Acromion process
Head of humerus
Coracoid process
FIG. 16.2
1. Acromion process
2. Coracoid process
3. Spine
4. Glenoid cavity (fossa)
5.
6.
7.
Acromion process
Coracoid process
Glenoid cavity
8.
9.
10.
11.
12.
13.
14.
Capitulum
Trochlea
Greater tubercle
Anatomical neck
Surgical neck
Medial epicondyle
Olecranon fossa
5.
6.
7.
Trochlear notch
Coronoid process
Head of ulna
FIG. 16.5
1. Olecranon process
2. Humerus
3. Olecranon fossa
4.
5.
6.
Head of radius
Radius
Ulna
FIG. 16.6
1. Scaphoid
2. Capitate
3. Trapezoid
4. Trapezium
5. Carpals (carpus)
6. Metacarpals (metacarpus)
7.
8.
9.
10.
11.
12.
Phalanges
Lunate
Triquetrum
Proximal phalanx
Middle phalanx
Distal phalanx
5.
6.
7.
8.
Spine
Acromion process
Coracoid process
Head
FIG. 16.4
1. Head of radius
2. Radial tuberosity
3. Styloid process of radius
4. Olecranon process
19
PART B
1. a
2. b
3. b
4.
5.
6.
b
c
d
7.
8.
9.
b
a
b
9.
10.
11.
12.
13.
14.
15.
16.
Clavicle
Scapula
Rib
Phalanges
Metacarpals
Carpals
Distal phalanx
Proximal phalanx
7.
8.
9.
10.
11.
12.
Trapezoid
Trapezium
Metacarpals
Proximal phalanges
Middle phalanges
Distal phalanges
20
10. e
11. a
12. f
LABORATORY EXERCISE 17
PELVIC GIRDLE AND LOWER LIMB
Figure Labels
FIG. 17.1
1.
2.
FIG. 17.2
1. Ilium
2. Posterior superior iliac spine
3. Greater sciatic notch
4. Ischial spine
5. Lesser sciatic notch
6. Ischium
3.
Coccyx
7.
8.
9.
10.
11.
12.
Ischial tuberosity
Iliac crest
Anterior superior iliac spine
Acetabulum
Pubis
Obturator foramen
5.
6.
7.
8.
Lateral epicondyle
Lesser trochanter
Lateral condyle
Medial condyle
FIG. 17.4
1. Head of fibula
2. Fibula
3. Lateral malleolus
4. Medial condyle
5.
6.
7.
Tibial tuberosity
Tibia
Medial malleolus
FIG. 17.5
1. Calcaneus
2. Talus
3. Cuboid
4. Navicular
5. Lateral cuneiform
6. Intermediate cuneiform
7. Medial cuneiform
8.
9.
10.
11.
12.
13.
Proximal phalanx
Middle phalanx
Distal phalanx
Tarsals (tarsus)
Metatarsals (metatarsus)
Phalanges
4.
5.
6.
Ischial spines
Symphysis pubis
Iliac crest
7.
8.
9.
Tuberosity
Pubic arch
Obturator foramen
10. Sacroiliac
PART B
1. e
2. a
3. g
4.
5.
6.
a
f
f
7.
8.
9.
g
f
a
10. b
11. d
12. c
21
22
10.
11.
12.
13.
14.
15.
16.
17.
18.
Lateral condyle
Head of fibula
Fibula
Tibia
Talus
Calcaneus
Metatarsal
Proximal phalanx
Distal phalanx
7.
8.
9.
10.
11.
Navicular
Talus
Middle phalanges
Cuboid
Calcaneus
LABORATORY EXERCISE 18
JOINTS
4.
5.
e
a
6.
7.
8.
9.
Pivot (synovial)
Condyloid/ellipsoid (synovial)
Saddle (synovial)
Ball-and-socket (synovial)
PART C
1. a
2. b
3. e
4. d
5. d
6.
7.
8.
9.
10.
a
d (b and c also present)
f
c
c
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
Extension
Flexion
Extension
Flexion
Flexion
Extension
Flexion
Extension
Flexion
Extension
Flexion
Extension
Dorsiflexion
Plantar flexion
PART E
Table:
Type of Joint
Ball-and-socket
Hinge, gliding, and pivot
Ball-and-socket
Hinge (modified),
condyloid, and gliding
Bones Included
Humerus, scapula
Humerus, radius, ulna
Femur, coxa
Femur, tibia, patella
23
LABORATORY EXERCISE 19
SKELETAL MUSCLE STRUCTURE
Figure Labels
FIG. 19.4
1. Coracoid process
2. Origins of biceps brachii (tendons)
3. Biceps brachii
4.
5.
6.
Radius
Insertion of biceps brachii (tendon)
Ulna
4.
5.
6.
f
g
b
3.
4.
Biceps brachii
Prime mover
7.
8.
9.
h
a
j
3.
4.
I band (light)
A band (dark)
5.
6.
Synergists
Prime movers
24
10. k
11. e
12. d
LABORATORY EXERCISE 20
MUSCLES OF THE FACE, HEAD, AND NECK
Figure Labels
FIG. 20.1
1. Temporalis
2. Occipitalis (epicranius)
3. Masseter
4. Sternocleidomastoid
5. Platysma
6.
7.
8.
9.
10.
Frontalis (epicranius)
Orbicularis oculi
Zygomaticus major
Orbicularis oris
Buccinator
FIG. 20.2
1. Lateral pterygoid
2.
Medial pterygoid
3.
Splenius capitis
PART A
1. Zygomaticus major
2. Buccinator
3. Orbicularis oris
4. Lateral pterygoid
5. Raise the jaw/elevate mandible (as in biting)
6. Medial
7.
8.
9.
10.
11.
12.
Lateral
Orbicularis oculi
Sternocleidomastoid
Splenius capitis
Platysma
Zygomaticus
PART B
1. Epicranius
2. Zygomaticus major
3. Masseter
4. Lateral pterygoid
5. Sternocleidomastoid
6.
7.
8.
9.
10.
Buccinator
Platysma
Temporalis
Splenius capitis
Semispinalis capitis
4.
5.
Orbicularis oris
Platysma
FIG. 20.3
1. Longissimus capitis (erector spinae)
2. Semispinalis capitis
Laboratory Report Answers
25
LABORATORY EXERCISE 21
MUSCLES OF THE CHEST, SHOULDER, AND UPPER LIMB
Figure Labels
FIG. 21.1
1. Trapezius
2. Deltoid
3. Latissimus dorsi
4. Levator scapulae
5. Supraspinatus
6.
7.
8.
9.
Infraspinatus
Teres minor
Teres major
Rhomboid major
FIG. 21.2
1. Pectoralis minor
2. Internal intercostal
3. Pectoralis major
4.
5.
Deltoid
Serratus anterior
FIG. 21.3a
1. Levator scapulae
2. Supraspinatus
3. Deltoid
4.
5.
6.
Teres minor
Teres major
Triceps brachii
FIG. 21.3b
1. Deltoid
2. Subscapularis
3.
4.
Biceps brachii
Brachialis
FIG. 21.4a
1. Pronator teres
2. Brachioradialis
3.
4.
FIG. 21.4b
1. Flexor carpi ulnaris
2. Extensor carpi ulnaris
3.
4.
4.
5.
6.
i
h
j
7.
8.
9.
a
b
l
PART B
1. Rhomboid major
2. Serratus anterior
3. Pectoralis minor
4. Coracobrachialis
5. Teres major
6. Subscapularis
10. k
11. d
12. f
7.
8.
9.
10.
11.
12.
Teres minor
Brachialis
Pronator teres
Brachioradialis
Flexor carpi radialis
Palmaris longus
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
Biceps brachii
Triceps brachii
Latissimus dorsi
Pectoralis major
Serratus anterior
Biceps brachii
Trapezius
Deltoid
Triceps brachii
Brachioradialis
LABORATORY EXERCISE 22
MUSCLES OF THE DEEP BACK, ABDOMINAL WALL, AND PELVIC OUTLET
Figure Labels
FIG. 22.1
1. Longissimus (intermediate group)
2. Spinalis (medial group)
3.
FIG. 22.2
1. External oblique
2. Internal oblique
3.
4.
Transversus abdominis
Rectus abdominis
FIG. 22.3
1. Bulbospongiosus
2. Ischiocavernosus
3. Superficial transversus perinei
4.
5.
6.
Levator ani
Gluteus maximus
External anal sphincter
FIG. 22.4
1. Ischiocavernosus
2. Bulbospongiosus
3. Superficial transversus perinei
4.
5.
6.
Levator ani
Gluteus maximus
External anal sphinchter
4.
PART B
1. Pelvic diaphragm
2. Anal canal and vagina
3. Support the pelvic viscera and provide sphincterlike
action in the anal canal and vagina (aid the levator ani)
4. Bulbospongiosus
5. Constrict the vagina
27
5.
6.
6.
7.
8.
9.
10.
Ischial tuberosity
Levator ani
Support the pelvic viscera
Ischial spine
Sphincter urethrae
LABORATORY EXERCISE 23
MUSCLES OF THE HIP AND LOWER LIMB
Figure Labels
FIG. 23.1
1. Tensor fasciae latae
2. Sartorius
3. Rectus femoris
4. Vastus lateralis
5. Vastus medialis
6.
7.
8.
9.
Psoas major
Iliacus
Adductor longus
Gracilis
4.
5.
6.
FIG. 23.3
1. Adductor magnus
2. Gracilis
3. Gluteus medius
4. Gluteus maximus
5.
6.
7.
Biceps femoris
Semitendinosus
Semimembranosus
FIG. 23.4
1. Fibularis (peroneus) longus
2. Tibialis anterior
3.
FIG. 23.5
1. Gastrocnemius
2. Soleus
3. Fibularis (peroneus) longus
4.
5.
Tibialis anterior
Extensor digitorum longus
2.
Soleus
PART A
1. e
2. b
3. f
4. h
5. c
6.
7.
8.
9.
10.
d
j
a
g
i
PART B
1. Gluteus medius and gluteus minimus
2. Adductor magnus
3. Sartorius
4. Gastrocnemius
5. Tensor fasciae latae
6.
7.
8.
9.
10.
Vastus lateralis
Semitendinosus
Vastus medialis
Flexor digitorum longus
Tibialis anterior
5.
6.
7.
8.
Vastus medialis
Tibialis anterior
Gastrocnemius
Soleus
FIG. 23.2
1. Gluteus medius
2. Gluteus maximus
3. Biceps femoris
FIG. 23.6
1. Gastrocnemius
Laboratory Report Answers
28
LABORATORY EXERCISE 24
FETAL PIG DISSECTION: MUSCULATURE
Instructional Suggestions
1.
Due to changes from fewer small pig farms to large scale pig production operations, fewer pigs are available. The increased
costs can be reduced by dissecting smaller fetal pigs (musculature becomes more difficult), increasing the group size, and
reusing dissected pigs with future classes.
2.
You may want to reduce the amount of time spent in dissecting the fetal pig muscles by having the students dissect only
selected areas of the fetal pig. For example, you might have them complete Procedure E (muscles of the thorax), Procedure
F (muscles of the abdominal wall), Procedure G (muscles of the shoulder and back), and Procedure I (muscles of the hip and
hindlimb), omitting the other sections.
3.
Sternohyoid, mylohyoid
PART B
1. Pectoralis major
2. Pectoralis minor
3. Superficial pectoral
4. Posterior deep pectoral
5. Anterior deep pectoral
6.
7.
8.
9.
External oblique
Internal oblique
Transversus abdominis
Rectus abdominis
PART C
1. Clavotrapezius (ventral portion of brachiocephalic)
2. Acromiotrapezius
3. Spinotrapezius
4.
5.
Supraspinatus
Infraspinatus
PART D
1. Biceps brachii
2. Brachialis
3.
Triceps brachii
9.
10.
11.
12.
13.
14.
15.
16.
Infraspinatus
Teres major
Triceps brachii
Latissimus dorsi
Gluteus medius
Gluteus maximus
Biceps femoris
Gastrocnemius
29
LABORATORY EXERCISE 25
NERVOUS TISSUE AND NERVES
Figure Labels
FIG. 25.1
1. Nucleus
2. Nucleolus
3. Nodes of Ranvier
4. Schwann cell
FIG. 25.2
1. Schwann cell nucleus
2. Myelin (of Schwann cell)
5.
6.
7.
Dendrites
Cell body
Axon (nerve fiber)
3.
4.
3.
4.
h
f
5.
6.
e
d
7.
8.
a
b
PART B
1. d
2. f
3.
4.
h
g
5.
6.
e
a
7.
8.
c
b
PART C
1. (sketch)
2. (sketch)
3. (sketch)
PART D
1. (sketch)
2. (sketch)
30
LABORATORY EXERCISE 26
MENINGES AND SPINAL CORD
Figure Labels
FIG. 26.1
1. Subarachnoid space
2. White matter
3. Dorsal root of spinal nerve
4. Ventral root of spinal nerve
5.
6.
7.
Body of vertebra
Epidural space
Gray matter
FIG. 26.2
1. Posterior horn
2. Lateral funiculus
3. Anterior horn
4. Posterior funiculus
5. Posterior median sulcus
6.
7.
8.
9.
Central canal
Gray commissure
Anterior median fissure
Anterior funiculus
3.
4.
d
f
5.
6.
g
a
PART B
1. Spinal nerves
2. Cervical
3. Lumbar
4. Posterior median sulcus
5. Horns
PART C
1. b
2. e
3. a
PART D (FIG. 26.4)
1. Dorsal root of spinal nerve
2. White matter
3. Ventral root of spinal nerve
4. Posterior median sulcus
31
7.
6.
7.
8.
9.
10.
Anterior
Gray commissure
Central canal
Funiculi
Meninges
4.
5.
d
c
5.
6.
7.
8.
Gray matter
Dorsal root ganglion
Anterior median fissure
Central canal
LABORATORY EXERCISE 27
REFLEX ARC AND REFLEXES
Figure Labels
FIG. 27.1
5
3
4
1
2
7.
8.
9.
10.
11.
12.
PART B
1. Table:
Response Observed
Extension of leg at the knee joint
Plantar flexion of the foot
Flexion of forearm at the elbow joint or slight biceps twitch
Extension of forearm at the elbow joint or slight triceps twitch
Plantar flexion of the foot and flexion of toes
2.
Quadriceps femoris
Sensory
Skin
Flexor
Flexion
Babinski
Effector Involved
Quadriceps femoris
Gastrocnemius and soleus
Biceps brachii
Triceps brachii
Gastrocnemius, soleus, and flexor digitorium longus
The quadriceps femoris is stretched, stimulating stretch receptors (muscle spindles) within the muscle. As a result, impulses
pass along sensory neurons into the spinal cord and synapse with a motor neuron. Motor impulses travel out of the cord on
nerve fibers that lead to the quadriceps femoris. Muscle fibers contract, and the leg is extended at the knee joint.
32
LABORATORY EXERCISE 28
BRAIN AND CRANIAL NERVES
Figure Labels
FIG. 28.1
12
1
8
10
4
3
6
11
5
7
9
2
FIG. 28.2
1. Frontal lobe
2. Temporal lobe
3.
4.
Parietal lobe
Occipital lobe
FIG. 28.3
1. Motor area for voluntary muscle control
2. Motor speech area (Brocas area)
3. Auditory area
4.
5.
6.
FIG. 28.4
1. (I) Olfactory nerve
2. (II) Optic nerve
3. (III) Oculomotor nerve
4. (IV) Trochlear nerve
5. (V) Trigeminal nerve
6. (VI) Abducens nerve
7.
8.
9.
10.
11.
12.
7.
8.
9.
10.
11.
12.
i
l
h
e
g
b
Major Functions
Coordinates speech
Regulates heartbeat
Relays impulses to and from cerebellum
Provides motor pathways between cerebrum and lower parts
Contains visual and auditory reflex centers
Controls voluntary movements of eyes and eyelids
Regulates body temperature and many visceral activities
Controls emotional feelings
Regulates rate and depth of breathing
Activates state of wakefulness
Relays sensory impulses to cerebral cortex
Controls diameter of blood vessels and blood pressure
6.
7.
8.
9.
10.
Pons
Medulla oblongata
Brainstem
Cerebrum
Cerebellum
33
PART D
1. Vestibulocochlear (VIII)
2. Facial (VII), glossopharyngeal (IX)
3. Optic (II)
4. Olfactory (I)
5. Vestibulocochlear (VIII)
6. Trigeminal (V)
7. Trigeminal (V)
8. Oculomotor (III)
9.
10.
11.
12.
13.
14.
15.
Oculomotor (III)
Oculomotor (III)
Oculomotor (III), trochlear (IV), abducens (VI)
Facial (VII), glossopharyngeal (IX)
Accessory (XI)
Vagus (X), accessory (XI), hypoglossal (XII)
Glossopharyngeal (IX), vagus (X), accessory (XI),
hypoglossal (XII)
34
LABORATORY EXERCISE 29
DISSECTION OF THE SHEEP BRAIN
Instructional Suggestion
Rather than have students dissect sheep brains, you might want to provide the class with samples of whole sheep brains and
sectioned brains for examination. This should extend the use of the available specimens.
Laboratory Report Answers
PART A
1. The human cerebral hemispheres are relatively
5.
larger than those of the sheep.
2. There are more convolutions and sulci in the
6.
human cerebrum.
3. The human cerebrum with its larger size and
7.
greater number of convolutions is more complex
and thus able to carry on more complex functions.
4. The human cerebellum is divided in the midline (vermis)
into two hemispheres, whereas the sheep cerebellum is
not divided.
The olfactory bulbs of the sheep brain are larger than those of
the human brain.
The olfactory, optic, and trigeminal nerves seem to be most
highly developed in the sheep brain.
The senses of smell and sight and the sensory functions
associated with the trigeminal nerve are highly developed.
35
LABORATORY EXERCISE 30
RECEPTORS AND GENERAL SENSES
Laboratory Report Answers
PART A
1. Chemoreceptors
2. Pain
3. Thermoreceptors
4. Photoreceptors
5. Referred
PART B
1. (experimental results)
2. (experimental results)
PART C
1. (experimental results)
PART D
1. (experimental results)
2. (experimental results)
6.
7.
8.
9.
10.
Sensory
Touch
Pressure
Heat
Cold
3.
2.
3.
36
LABORATORY EXERCISE 31
SMELL AND TASTE
Figure Labels
FIG. 31.1
6
5
3
7
FIG. 31.3
6
1
4
1
2
4
3
5
2
6.
7.
8.
9.
Olfactory
Frontal
Sensory adaptation
Odorless
PART B
1. (experimental results)
2. (experimental results)
3.
Epithelial
Taste pore
Taste hairs (microvilli)
Water
5.
6.
7.
8.
Receptor
Acids
Salts
Bitter
PART D
1. (experimental results)
37
LABORATORY EXERCISE 32
EAR AND HEARING
Figure Labels
FIG. 32.1
1. Auricle
2. Malleus
3. Incus
4. Semicircular canals
5. Stapes
FIG. 32.2
4
6
6.
7.
8.
9.
10.
11.
5
3
Cochlea
Vestibulocochlear nerve
Oval window
Tympanic membrane (eardrum)
Auditory (Eustachian) tube
External acoustic meatus
1
2
FIG 32.3
1. Tectorial membrane
2. Hair cells (receptor cells)
3.
4.
4.
5.
6.
h
c
b
7.
8.
9.
l
i
e
4.
5.
Basilar membrane
Scala tympani
3.
4.
(experimental results)
Answers will vary.
38
10. f
11. a
12. g
LABORATORY EXERCISE 33
EQUILIBRIUM
6.
7.
8.
9.
10.
Ampulla
Crista ampullaris
Cupula
Inertia
Cerebellum
PART B
1. a. The eyes, inner ears, and proprioceptors provide information needed to maintain equilibrium when the eyes are open.
b. The inner ears and proprioceptors provide such information when the eyes are closed.
2. a. (experimental results)
b. Probably yes; this demonstrates the importance of visual information in maintaining equilibrium.
c. With the eyes closed, such a person would receive very little sensor information needed to maintain equilibrium.
3. a. (experimental results)
b. Answers will vary.
39
LABORATORY EXERCISE 34
EYE STRUCTURE
Figure Labels
FIG. 34.1
1. Lacrimal gland
2. Superior and inferior canaliculi
3.
4.
Lacrimal sac
Nasolacrimal duct
FIG. 34.2
1. Superior oblique
2. Superior rectus
3. Medial rectus
4.
5.
6.
Lateral rectus
Inferior rectus
Inferior oblique
FIG. 34.3
1. Pupil
2. Iris
3. Ciliary body
4. Suspensory ligaments
5. Lens
6. Cornea
7. Aqueous humor
8. Anterior cavity
9.
10.
11.
12.
13.
14.
15.
16.
Retina
Choroid coat
Sclera
Vitreous humor
Fovea centralis
Optic nerve
Optic disc
Posterior cavity
10.
11.
12.
13.
i
k
c
Cornea, aqueous humor, pupil of iris, lens, vitreous humor,
retina
14. More rods than cones; rods stimulated by dim light; cones
enable perception of color [other answers could be acceptable.]
5.
7.
The dark pigment absorbs excess light and keeps the eye dark
dark inside.
The lens is biconvex, flexible, and transparent. It may be firm
and opaque in a preserved eye.
The vitreous humor is a transparent, jellylike fluid.
7.
8.
9.
10.
11.
Vitreous humor
Optic disc
Optic nerve
Choroid coat/middle tunic
Sclera/outer tunic
6.
40
LABORATORY EXERCISE 35
VISUAL TESTS AND DEMONSTRATIONS
41
LABORATORY EXERCISE 36
ENDOCRINE HISTOLOGY AND DIABETIC PHYSIOLOGY
Instructional Suggestions
1. The responses and recovery to insulin shock work faster if the fish selected is very small (total length about 1). The species
of fish selected does not seem significant so try a fish species that is readily available.
2. HumulinR administered in 200 units usually will suffice to induce insulin shock within about 5 minutes. If the responses are
too slow, try an increase to 400 units. Consult with your local pharmacist or veterinarian to purchase insulin.
3. If the recovery from insulin shock is too prolonged using a 10% glucose solution, try a 20% glucose solution.
Figure Labels
FIG. 36.1
1. Hypothalamus
2. Pituitary gland
3. Parathyroid glands
4. Testis (male)
5. Pineal gland
6.
7.
8.
9.
10.
Thyroid gland
Thymus
Adrenal gland
Pancreas
Ovary (female)
Type 1 Diabetes
Early age or adult
Rapid
10-15%
Below normal
Destroyed
Present
Heredity
Insulin administration
Hyperglycemia
Type 2 Diabetes
Usually over 40
Slow
85-90%
Normal or increased
Not Destroyed
Absent
Heredity, obesity, lack of exercise
Avoid foods that stimulate insulin
production, weight control, exercise,
medications
Hyperglycemia
PART C
1. (experimental results)
2. (experimental results)
The amount of time until insulin shock occurs varies with the type of insulin used and the size of the fish selected for the
experiment. Rapid or irregular swimming, gill cover, and mouth movements usually are noted within about 5-10 minutes.
3. (experimental results)
Recovery from insulin shock usually occurs within about 5-10 minutes.
42
LABORATORY EXERCISE 37
BLOOD CELLS
Warning
Because of the possibility of blood-borne infections being transmitted from one student to another if blood slides are prepared in
the classroom, it is suggested that commercially prepared blood slides be used in this exercise. The instructor, however, may
wish to demonstrate the procedure for preparing such a slide. Mammal blood other than human or contaminant-free human
blood is suggested as a substitute for collected blood.
Laboratory Report Answers
PART A
1. Erythrocytes
2. Biconcave
3. Transporting and exchanging gases (O2 and CO2)
4. Hemoglobin
5. Oxyhemoglobin
6. Nucleus
7. Leukocytes
8. Granulocytes
9. Agranulocytes
10.
11.
12.
13.
14.
15.
16.
17.
18.
Neutrophil
Neutrophils
Eosinophils
Basopils
Monocytes
Lymphocytes
Collagen
Serotonin
Hemocytoblasts (hematopoietic stem cells)
2.
PART B
(sketches)
PART C
1. (experimental results)
43
LABORATORY EXERCISE 38
BLOOD TESTINGA DEMONSTRATION
Warning
Because of the possibility of blood-borne infections being transmitted from one student to another during blood-testing
procedures, it is suggested that the following demonstrations be performed by the instructor. Mammal blood other than human or
contaminant-free human blood is suggested as a substitute for collected blood.
Laboratory Report Answers
PART A
(demonstration results)
PART B
1. Answers will vary.
3.
2. Various forms of anemia and severe hemorrhage will
produce a decreased red blood cell percentage.
PART C
1. Answers will vary.
3.
2. Iron-deficiency anemia, lack of certain amino acids or
vitamin B12, pregnancy, severe hemorrhage, excessive
menstrual flow, or excessive fluid intake may cause a
decreased hemoglobin content.
PART D
1. Answers will vary.
3.
2. Anemia, leukemia, and severe hemorrhage may cause
a decreased red blood cell count.
PART E
1. Answers will vary.
3.
2. Aplastic anemia and adverse drug reactions may cause
a decreased white blood cell count.
44
LABORATORY EXERCISE 39
BLOOD TYPING
Warning
Because of the possibility of blood-borne infections being transmitted from one student to another if blood-testing is preformed in
the classroom, it is suggested that commercially prepared blood-typing kits, containing virus-free human blood, be used for ABO
blood typing. The instructor may wish to demonstrate Rh blood typing. Simulated blood-typing kits are suggested as a substitute
for collected blood.
5.
6.
7.
8.
PART B
1. (experimental results)
PART C
1. Rhesus monkey
2. Antigen D
3. Rh-negative
4.
5.
6.
PART D
1. (demonstration results)
Anti-B
Anti-A
AB
O
Rh-negative
Clump (agglutinate)
Rh-positive
45
LABORATORY EXERCISE 40
HEART STRUCTURE
Instructional Suggestions
1. You may want to have the students use colored
3.
pencils to color the features of the heart and blood
vessels in figure 40.3. This activity should help them
observe the illustrations more carefully and locate the 4.
various features shown from different views in the
figures. They can check their work by referring to the
corresponding figures in the textbook, which are
presented in full color.
2. Instead of using preserved sheep hearts, you might
want to provide fresh pig hearts for dissection.
Figure Labels
FIG. 40.1
1. Aorta
2. Superior vena cava
3. Right atrium
4. Right coronary artery
5. Right ventricle
6. Inferior vena cava
FIG. 40.2
1. Aorta
2. Left pulmonary artery
3. Left pulmonary veins
4. Left atrium
5. Left ventricle
FIG. 40.3
1. Aorta
2. Superior vena cava
3. Aortic valve
4. Right atrium
5. Tricuspid valve
6. Chordae tendineae
7. Inferior vena cava
8. Left pulmonary artery
9. Pulmonary trunk
7.
8.
9.
10.
11.
6.
7.
8.
9.
10.
Apex
Superior vena cava
Right atrium
Inferior vena cava
Right ventricle
10.
11.
12.
13.
14.
15.
16.
17.
7.
8.
9.
10.
11.
12.
e
g
i
b
c
a
7.
8.
9.
10.
11.
Interventricular septum
Left atrium
Mitral (biscupid) valve
Left ventricle
Apex
46
PART C
1. The tricuspid valve is composed of three relatively
4.
large cusps that contain chordae tendineae; the
pulmonary valve is made up of three smaller pocketlike cusps that lack chordae tendineae.
2. The cusps of the tricuspid valve move upward into a
horizontal position and close the opening between the 5.
right atrium and the right ventricle.
3. The chordae tendineae and papillary muscles prevent the
cusps of the tricuspid and mitral (bicuspid) valves from
swinging into the atria when the ventricles contract.
47
LABORATORY EXERCISE 41
CARDIAC CYCLE
Laboratory Report Answers
PART A
1. Systole
2. Diastole
3. Closed
4. Open
5.
6.
7.
8.
Ventricles
A-V valves
Pulmonary and aortic valves (semilunar valves)
Murmur
PART B
1. (experimental results)
2.
(experimental results)
7.
8.
9.
Ventricles
Ventricles
Atrial repolarization occurs at the same time that the
ventricular fibers depolarize. The QRS complex indicating
ventricular depolarization obscures any recording of the atrial
repolarization.
5.
PART C
1. S-A (sinoatrial; sinu-atrial)
2. A-V (atrioventricular)
3. A-V bundle (bundle of His)
4. Purkinje fibers
5. Electrocardiogram
6. Atria
PART D
1. (labeled ECG recordings)
2. Answers will vary.
3. Normal is 0.12-0.20 sec.
4. The P-Q (P-R) interval indicates the time it takes
for the atria to depolarize and the cardiac impulse
to reach the A-V node.
6.
48
LABORATORY EXERCISE 42
BLOOD VESSELS
Instructional Suggestions
If live frogs are not available for the microscopic observation of blood vessels, you might want to provide small goldfish. The
head of a fish can be wrapped loosely in wet cotton to keep its gills moist, and the fish can be placed on a glass plate on the stage
of a microscope. If its tail is spread out beneath the microscope slide, the blood vessels can be observed with low- and highpower magnification. However, if the fish is not returned to water within a few minutes, it will likely die.
Figure Labels
FIG. 42.1
1. Tunica interna
2. Tunica media
FIG. 42.2
1
4
3.
Tunica externa
3
2
7.
8.
9.
10.
11.
12.
Sphincters
Diffusion
Hydrostatic
Osmotic
Lymphatic
Valves
3.
The tunica interna and tunica externa are similar in the artery
and vein. The tunica media of the artery contains relatively
greater amounts of smooth muscle and elastic tissue than that of
the vein.
49
LABORATORY EXERCISE 43
PULSE RATE AND BLOOD PRESSURE
Instructional Suggestions
The following suggestions should be considered when trying to obtain an accurate blood pressure:
1.
2.
3.
The room environment should have a moderate temperature and be quiet (no talking).
The client needs to be relaxed and comfortable. A temporary increase in blood pressure could exist from smoking, pain,
anxiety, or a full urinary bladder.
Palpate the pulse first so that you are certain to pump the cuff high enough to not miss the first tapping sound. It also assures
that you do not pump the cuff so high that we alter the blood pressure when releasing air.
5.
6.
7.
8.
Sphygmomanometer
Millimeters of mercury (mm Hg)
Systolic
Brachial
PART B
1. (test results)
2.
PART C
1. (test results)
2. (test results)
3.
4.
50
LABORATORY EXERCISE 44
ARTERIES AND VEINS
Figure Labels
FIG. 44.1
1. Superior vena cava
2. Pulmonary trunk
3. Inferior vena cava
4.
5.
6.
Pulmonary veins
Pulmonary artery
Aorta
7.
8.
9.
10.
11.
FIG. 44.3
1. Superficial temporal artery
2. Internal carotid artery
3. External carotid artery
4. Vertebral artery
5.
6.
7.
8.
Subclavian artery
Facial artery
Common carotid artery
Brachiocephalic artery
FIG. 44.4
1. Subclavian artery
2. Axillary artery
3. Deep brachial artery
4.
5.
6.
Brachial artery
Radial artery
Ulnar artery
FIG. 44.5
1. Common iliac artery
2. External iliac artery
3. Deep femoral artery
4. Popiteal artery
5. Abdominal aorta
6.
7.
8.
9.
FIG. 44.6
1. External jugular vein
2. Subclavian vein
3. Internal jugular vein
4.
5.
Vertebral vein
Brachiocephalic vein
5.
6.
7.
8.
Brachiocephalic veins
Subclavian vein
Superior vena cava
Azygos vein
FIG. 44.8
1. Subclavian vein
2. Right brachiocephalic vein
3. Axillary vein
4. Cephalic vein
5. Basilic vein
6.
7.
8.
9.
Brachial vein
Median cubital vein (antecubital vein)
Radial vein
Ulnar vein
FIG. 44.9
1. Hepatic portal vein
2. Superior mesenteric vein
3. Gastric vein (right)
4.
5.
Splenic vein
Inferior mesenteric vein
FIG. 44.7
1. Internal jugular vein
2. Axillary vein
3. Cephalic vein
4. External jugular vein
51
FIG. 44.10
1. Common iliac vein
2. External iliac vein
3. Interior vena cava
4. Internal iliac vein
5.
6.
7.
8.
Femoral vein
Great saphenous vein
Popliteal vein
Anterior tibial vein
7.
8.
9.
10.
11.
12.
k
h
e
f
l
i
5.
6.
7.
Brachial artery
External iliac artery
Left and right pulmonary arteries
PART C
1. a
2. b
3. d
4. e
5.
6.
7.
8.
h
c
g
f
PART D
1. Right brachiocephalic vein
2. Popliteal vein
3. Common iliac vein
4. Basilic vein
5.
6.
7.
8.
Femoral vein
Hepatic portal vein
Pulmonary veins
Renal vein
9.
10.
11.
12.
13.
14.
15.
Subclavian vein
Pulmonary vein
Inferior vena cava
Aorta
Common iliac vein
Common iliac artery
Femoral artery
52
LABORATORY EXERCISE 45
FETAL PIG DISSECTION: CARDIOVASCULAR SYSTEM
Laboratory Report Answers
PART A
1. The parietal pericardium forms a relatively thick, tough sac that encloses the heart. It is attached to the large blood vessels at
the base of the heart and to the diaphragm.
2. The walls of the atria are much thinner than those of the ventricles. The wall of the left ventricle is much thicker than that of
the right ventricle.
3. Wall thickness is related to the force of its contraction and the amount of pressure it imparts to the blood inside a heart
chamber. The left ventricle has the thickest wall, contracts with the greatest force, and creates the greatest amount of blood
pressure in the heart chambers. The left ventricle is the pump for the systemic circuit.
4. In the human, the right common carotid artery branches from the brachiocephalic artery, whereas the left common carotid
artery comes directly from the aortic arch. In the fetal pig, both common carotid arteries branch from the brachiocephalic
artery.
5. In the human, the aorta divides to form the two common iliac arteries, which in turn give rise to external and internal iliac
arteries. In the fetal pig, the aorta divides to form the external iliac arteries, and the internal iliac arteries branch from the
aorta independently. (The umbilical arteries originate from the internal iliac arteries.)
PART B
1. In the human, the internal jugular vein is somewhat larger than the external jugular vein. In the fetal pig, the external jugular
vein and the internal jugular vein are a similar size.
2. Answers will vary.
53
LABORATORY EXERCISE 46
LYMPHATIC SYSTEM
Figure Labels
FIG. 46.1
6
3
2
4
8
1
5
7
FIG. 46.2
1
2
4
6
3
5
4.
5.
6.
Valves
Lymph nodes
Thoracic (left lymphatic) duct
6.
7.
8.
9.
Tonsils
Peyers patches
Scalp, face, nasal cavity, and pharynx
Inguinal
5.
6.
7.
8.
Blood
White pulp
Red pulp
Macrophages
PART D
(sketches)
54
LABORATORY EXERCISE 47
DIGESTIVE ORGANS
Figure Labels
FIG. 47.1
1. Lip
2. Hard palate
3. Soft palate
4. Uvula
5.
6.
7.
Palatine tonsil
Tongue
Vestibule
FIG. 47.2
1. Parotid gland
2. Masseter muscle
3. Tongue
4.
5.
Sublingual gland
Submandibular gland
FIG. 47.3
1. Enamel
2. Dentin
3. Root
4.
5.
6.
Crown
Gingiva
Root canal
FIG. 47.5
1. Pharyngeal tonsils (adenoids)
2. Opening of auditory tube (Eustachian tube)
3. Nasopharynx
4. Palatine tonsils
5.
6.
7.
8.
Oropharynx
Lingual tonsils
Epiglottis
Laryngopharynx
FIG. 47.7
1. Esophagus
2. Rugae
3. Pyloric sphincter
4. Duodenum
5. Lower esophageal sphincter (cardiac sphincter)
6.
7.
8.
9.
Fundic region
Cardiac region
Body region
Pyloric region
FIG. 47.8
4
2
5
6
1
3
7
FIG. 47.10
1. Cystic duct
2. Gallbladder
3. Duodenum
4. Hepatic duct (common)
5.
6.
7.
FIG. 47.11
10
1
3
7
2
6
9
8
5
4
55
4.
5.
6.
a
h
k
PART B
1. Nasopharynx
2. Oropharynx
3. Laryngopharynx
4. Nasopharynx
PART C
1. Cardiac, fundic, body, and pyloric regions
2. Pyloric sphincter
3. Chief cells
4. Parietal cells
5. Pepsin
6. Gastrin
PART D
1. d
2. b
3. e
4.
5.
6.
a
f
i
7.
8.
9.
i
d
l
10. e
11. a
12. b
5.
6.
7.
Trachea
Mucus
The esophagus provides a passageway for food from the
pharynx to the stomach.
7.
8.
Chyme
The stomach receives food from the esophagus, mixes it with
gastric juice, initiates the digestion of protein, does limited
amount of absorption, and moves food (chyme) into the small
intestine.
7.
8.
9.
c
h
g
6.
7.
8.
PART E
(sketch)
PART F
1. Duodenum, jejunum, ileum
2. A mesentery supports and suspends organs. It
contains blood vessels, lymphatic vessels, and nerves
that supply the organs.
3. Lacteal
4. Peptidases, sucrase, maltase, lactase, intestinal lipase,
enterokinase
5. Ascending colon, transverse colon, descending colon,
sigmoid colon
9.
56
LABORATORY EXERCISE 48
FETAL PIG DISSECTION: DIGESTIVE SYSTEM
Laboratory Report Answers
PART A
1. The major salivary glands (parotid, submandibular, and sublingual) in the human and the pig occupy similar locations.
2. The jaw of the baby pig has six incisors, two canines, eight premolars, and zero molars; the jaw of the human child has four
incisors, two canines, zero premolars, and four molars.
3. The pigs canine teeth are adapted for an omnivorous diet as in humans. The pig has larger canine teeth than in the human
that aid in grasping food.
4. The uvula is missing in the pig.
5. The transverse ridges help to hold food.
6. Many papillae are near the tip and base of the tongue in the pig. The papillae are sensory and function in food manipulation.
PART B
1. The peritoneum is the membrane that lines the abdominal cavity and covers the abdominal organs. Double-layered folds in
this membrane form the mesentery that supports the abdominal organs.
2. The inner lining of the stomach is folded to form many ridges called rugae.
3. The pigs liver has five lobes; the human liver has four.
4. The pigs large intestine is on the left side and is composed of a predominant spiral colon. The human large intestine has an
ascending colon on the right side which is connected to a descending colon on the left side by a transverse colon.
5. The appendix is missing in the pig.
57
LABORATORY EXERCISE 49
ACTION OF A DIGESTIVE ENZYME
Instructional Suggestion
For this experiment to work, it is very important to obtain amylase that is free of sugar. Some of the amylase sold by laboratory
suppliers in 2005 contained sugar, as can be determined by the control in tube 1 of this experiment. Wards Natural Science does
handle amylase (alpha amylase from Bacillus subtilis; catalog #944W4602) that is free of sugar, and several other companies
plan to add this product to their catalogs. If in doubt, call the supply company and consult with a person in technical support.
Keep any of the unused amylase frozen.
Laboratory Report Answers
PART A
1. Expected experimental results:
Tube
Starch
Sugar
1
(varies)
2.
a. Testing the amylase solution for the presence of starch and sugar demonstrates the negative results of the tests.
b. Tube 2 demonstrates that starch will not change to sugar when warmed to 37C (98.6F).
c. The change of starch to sugar is a result of the action of the amylase in tube 3.
PART B
1. Expected experimental results:
Tube
Starch
Sugar
4
(varies)
(varies)
2.
a. Amylase is slow to act or inactive in low temperature and high temperature. Its optimum temperature is near 37C
(98.6F).
b. The tubes in which digestion failed to occur could be placed in the 37C (98.6F) water bath. If digestion occurred at this
temperature, the enzyme was not destroyed by the previous treatment.
58
LABORATORY EXERCISE 50
RESPIRATORY ORGANS
Figure Labels
FIG. 50.1
1. Nostril (external naris)
2. Oral cavity
3. Epiglottis
4. Larynx
5. Right primary bronchus
6. Right lung
7.
8.
9.
10.
11.
Frontal sinus
Nasal cavity
Pharynx
Trachea
Left lung
FIG. 50.2
1. Frontal sinus
2. Nostril (external naris)
3. Auditory (Eustachian) tube opening
4. Uvula
5. Palatine tonsil
6. Epiglottis
7. Trachea
8.
9.
10.
11.
12.
13.
14.
FIG. 50.3
1. Epiglottis (epiglottic cartilage)
2. Thyroid cartilage
3. Cricoid cartilage
4.
5.
6.
FIG. 50.4
1. Epiglottis
2. Glottis
3.
4.
7.
8.
9.
j
f
g
4.
5.
6.
a
e
d
10. c
PART B
(sketches)
PART C
1. The sticky mucus is secreted into the upper and
3.
lower respiratory tract, which will trap particles of
dust and microorganisms.
2. The cilia create a current of mucus toward the pharynx.
The mucus contains entrapped particles that are usually
swallowed.
59
LABORATORY EXERCISE 51
FETAL PIG DISSECTION: RESPIRATORY SYSTEM
60
LABORATORY EXERCISE 52
BREATHING AND RESPIRATORY VOLUME AND CAPACITIES
3.
4.
e
f
5.
6.
7.
Surfactant
Internal intercostal
Contracts
5.
6.
d
h
PART C
1. (experimental results).
2. a. Answers will vary.
b. Answers will vary.
c. A measurement of residual volume is needed.
3. Answers will vary.
61
7.
8.
c
b
LABORATORY EXERCISE 53
CONTROL OF BREATHING
Laboratory Report Answers
PART A
1. Pons
2. Dorsal
3. Ventral
4. Carbon dioxide
5. Increases
6.
7.
8.
9.
Carbon dioxide
Bicarbonate ions
Reduced
Increased
PART B
1. (experimental results)
2. a. Hyperventilation lowers blood carbon dioxide levels, and the breathing center is stimulated to a lesser degree.
b. Air that is rebreathed has a higher concentration of carbon dioxide, and thus the breathing center is stimulated to a greater
degree.
c. Breath holding causes the blood carbon dioxide concentration to increase, stimulating the breathing center to a greater
degree.
d. Exercise causes an increase in the breathing rate. This is due in part to the joint reflex and in part to an increasing
concentration of blood carbon dioxide.
3. a. It is difficult for a person to breathe normally while thinking about it.
b. Depth of breath can be measured by breathing into an instrument that measures air volumes.
62
LABORATORY EXERCISE 54
KIDNEY STRUCTURE
Figure Labels
FIG. 54.1
1. Kidney
2. Ureter
3.
4.
Urinary bladder
Urethra
FIG. 54.2
1. Minor calyx
2. Major calyx
3. Renal pelvis
4. Renal papilla
5. Ureter
6.
7.
8.
9.
10.
Renal pyramid
Renal column
Renal capsule
Renal medulla
Renal cortex
7.
8.
9.
10.
11.
Interlobular vein
Afferent arteriole
Distal convoluted tubule
Peritubular capillary
Collecting duct
6.
7.
8.
9.
c
d
g
b
FIG. 54.4
1. Glomerular capsule
2. Proximal convoluted tubule
3. Glomerulus
4. Efferent arteriole
5. Descending limb of the nephron loop
6. Ascending limb of the nephron loop
Laboratory Report Answers
PART A
1. e
2. a
3. h
4. f
5. i
PART B
1. A renal corpuscle is the cluster of capillaries (glomerulus) and the saclike structure (glomerular capsule) that surrounds it; a
renal tubule is the coiled tube that leads away from the glomerular capsule and empties into a collecting duct.
2. 3 5 2 4 1 6
3. 1 3 2 4 5
4. Blood enters the glomerulus through the afferent arteriole and leaves through the efferent arteriole. Because the afferent
vessel has a somewhat greater diameter than the efferent one, blood pressure is increased in the glomerulus.
5. The juxtaglomerular apparatus is a structure composed of densely packed epithelial cells of the distal convoluted tubule, and
smooth muscle cells within the walls between afferent and efferent arterioles, near the glomerulus. It regulates the flow of
blood through certain renal blood vessels and the secretion of renin.
PART C
(sketch)
PART D
(sketch)
63
LABORATORY EXERCISE 55
URINALYSIS
Instructional Suggestion
Because most students urine will produce negative results for glucose, protein, ketones, bilirubin, and hemoglobin, you may
want to provide samples of artificial urine (distilled water that contains weak concentrations of some of these substances). By
performing the urinalysis test on such samples, students will be able to obtain some positive results. Normal and abnormal
simulated urine specimens are suggested as a substitute for collected urine.
Laboratory Report Answers
PART A
1. (test results)
2. Answers will vary.
64
LABORATORY EXERCISE 56
FETAL PIG DISSECTION: URINARY SYSTEM
Laboratory Report Answers
1. In the human, the left kidney is usually higher than the right one; in the pig, the kidneys are at similar positions on both
sides. In both cases, the kidneys are retroperitoneal.
2. In the human, the adrenal glands are located on the superior ends of the kidneys; in the fetal pig, the adrenal glands are
located anteriorly and medially to the kidneys.
3. During fetal development the allantoic bladder eliminates wastes via the allantoic stalk. As a result, the mother eliminates
the metabolic wastes for the fetus until birth.
4. The wall of the urinary bladder is relatively thick, tough, and somewhat elastic. Its inner surface is irregular.
5. The termination of the urethra of a female pig is the urogenital sinus a short distance from the urogenital orifice. The human
urethra has its own external opening, the external urethral orifice (urinary meatus), just anterior to the vaginal orifice.
65
LABORATORY EXERCISE 57
MALE REPRODUCTIVE SYSTEM
Figure Labels
FIG. 57.1
1. Ductus deferens (vas deferens)
2. Urethra
3. Penis
4. Glans penis
5. Prepuce
6. Testis
7.
8.
9.
10.
11.
Scrotum
Seminal vesicle
Prostate gland
Bulbourethral gland
Epididymis
7.
8.
9.
10.
11.
12.
23
Acrosome
Epididymis
Alkaline
Glans penis
Ejaculation
FIG 57.2
6
1
4
3
7
5
2
Laboratory Report Answers
PART A
1. Spermatic
2. Testosterone
3. Gubernaculum
4. Inguinal canal
5. Epididymis
6. Meiosis (spermatogenesis)
PART B
1. (sketch)
2. (sketch)
3. (sketch)
4. a. Sustentacular cells support, nourish, and regulate the spermatogenic cells.
b. Spermatogenic cells give rise to sperm cells by meiosis (spermatogenesis).
c. Interstitial cells produce and secrete male sex hormones.
d. The epididymis stores sperm cells while they mature and propels them into the ductus deferens.
e. The corpora cavernosa and corpus spongiosum form three columns of erectile tissue that contain vascular spaces
(sinuses) that become engorged with blood during an erection. They also form most of the body of the penis.
66
LABORATORY EXERCISE 58
FEMALE REPRODUCTIVE SYSTEM
Figure Labels
FIG. 58.1
1. Uterine tube
2. Ovary
3. Uterus
4. Clitoris
5. Labium minus
6.
7.
8.
9.
10.
Labium majus
Vaginal orifice
Fimbriae
Cervix
Vagina
FIG. 58.2
1. Clitoris
2. Urethral orifice (meatus)
3. Vaginal orifice
4. Mons pubis
5.
6.
7.
8.
Labium majus
Labium minus
Perineum
Anus
FIG. 58.3
5
10
9
4
3
8
1
2
7
6
FIG. 58.4
1. Areola
2. Nipple
3. Lacriferous duct
4. Alveolar glands (mammary glands)
5. Adipose tissue
Laboratory Report Answers
PART A
1. Pelvic
2. Broad
3. Oogenesis
4. Follicular
5. First polar body
6. Ovulation
7.
8.
9.
10.
11.
12.
Infundibulum
Cervix
Endometrium
Smooth muscle
Hymen
Mons pubis
PART B
1. (sketch)
2. (sketch)
3. (sketch)
4. (sketch)
5. a. A mature follicle swells and ruptures under the influence of certain hormones. As this happens, the secondary oocyte
(egg cell) and follicular fluid escape from the ovary.
b. The cilia that line the uterine tube beat toward the uterus and help draw the secondary oocyte into the infundibulum of the
tube and continue to move it toward the uterus.
c. The uterine lining thickens and then it becomes glandular and vascular. If fertilization does not occur, the lining
disintegrates and sloughs away, creating the menstrual flow.
67
LABORATORY EXERCISE 59
FETAL PIG DISSECTION: REPRODUCTIVE SYSTEMS
Laboratory Report Answers
PART A
1. The fetal pig uterine tubes are small, coiled tubes near the ovaries that travel relatively short distances to join the uterine
horns. The human uterine tubes are much larger and longer, traveling from the region of the ovaries to the uterine body.
2. The human uterus contains a single chamber in which the offspring develop. The fetal pig uterus is Y-shaped with branches,
forming right and left uterine horns.
3. The uterine horns of the fetal pig provide room for many offspring to develop at one time.
4. In the fetal pig, the vagina and urethra open into a common urogenital sinus; in the human each of these organs has a
separate opening to the outside.
PART B
1. The ovaries and testes are homologous structures that originate near the dorsal abdominal wall posterior to the kidneys. In
the male fetus, the testes migrate through the inguinal canal by means of the gubernaculum until they reach the scrotum
which provides an environment better for spermatogenesis and sperm storage. Because fetal pigs were not full term
(gestation), the testes are located at some point within the inguinal canal.
2. In the fetal pig, the bulbourethral glands are large along the urethra near the proximal end of the penis; in the human, the
bulbourethral glands are the smallest of the accessory glands located also along the urethra near the proximal end of the
penis.
3. The prepuce of the fetal pig encloses the entire penis for protection. In the human, the prepuce or foreskin is vestigial and
only covers the glans of the penis. A circumcision is commonly performed on humans to remove the prepuce for sanitation
and other reasons.
68
LABORATORY EXERCISE 60
FERTILIZATION AND EARLY DEVELOPMENT
Instructional Suggestions
Sea urchin egg and sperm suspensions can be obtained from ripe sea urchins. The Sea Urchin Embryology Kit from Carolina
Biological Supply Company is one source of these materials.
Figure Labels
FIG. 60.1
1. Ovulation
2. Zygote
3.
4.
Morula (late)
Blastocyst
4.
5.
6.
Mitosis
Cleavage
Cilia
7.
8.
9.
Blastocyst
Embryo
Fetus
4.
5.
6.
f
d
b
7.
PART B
(sketches)
PART C
(sketches)
PART D
1. e
2. a
3. g
PART E
1. Amnion
2. Umbilical arteries
3. Amniochorionic membrane
4. Yolk sac
5. Allantois
6. Eighth
7. Embryonic
8. Amniotic
69
LABORATORY EXERCISE 61
GENETICS
Laboratory Report Answers
PART A
1. (experimental results)
2. (experimental results); Answers will vary.
PART B
1. Probability: 10/20 heads; 10/20 tails
(experimental results)
2. Probability: 8/24 number below 3
(experimental results)
3. Probability: 8/32 of two heads; 16/32 of a heads and a tails; 8/32 of two tails
(experimental results)
4. Probability: 8/32 of two odd numbers; 16/32 of an odd and an even number; 8/32 of two even numbers
(experimental results)
5. O = odd numbers
E = even numbers
6.
O
OO
E
OE
OE
EE
a. Yes
The results are closer to expected probabilities as trial numbers increase.
b. No
Each toss of a coin is an independent event.
c. or 50%
Each fertilization is a separate independent event.
d. 1/16
Each fertilization has the probability of for a girl. Therefore, the chances are = 1/16
PART C
1. a. Rr x rr
b and c.
2.
3.
R
Rr
r
rr
Rr
rr
IAIB
IAIB
IA i
IAi
70
4.
a. 1Ai x IBi
b and c.
B
I
i
5.
IA
A B
IBi
ii
I I
IAi
d. Genotypes: 25% IAIB; 25% IAi, 25% IBi, 25% ii (1:1:1:1 genotypic ratio)
Phenotypes: 25% AB; 25% A; 25% B; 25% O (1:1:1:1 genotypic ratio)
a. XHXh x XHY
b and c.
XH
Xh
H
H
H
X
X X
XHXh
Y
XHY
Xh Y
d. Genotypes: 25% XHXH; 25% XHXh; 25% XHY; 25% XhY (1:1:1:1 genotypic ratio)
Phenotypes: 50% normal females; 25% normal males; 25% males with hemophilia (2:1:1 phenotypic ratio). Note that all
females will have normal blood clotting.
Genotypes 1 WWEE; 2 WWEe; 2 WwEE; 4 WwEe; 1 WWee; 2 Wwee; 1 wwEE; 2 wwEe; 1 wwee (1:2:2:4:1:2:1:2:1
genotypic ratio)
Phenotypes: 9 widows peak, free earlobe; 3 widows peak, attached earlobe; 3 straight hairline, free earlobe; 1 straight
hairline, attached earlobe (9:3:3:1 phenotypic ratio)
71
LABORATORY EXERCISE 62
SKELETAL MUSCLE CONTRACTION
Laboratory Report Answers
PART A
1. h
2. a
3. c
4. i
5. b
6.
7.
8.
9.
f
g
d
e
PART B
1. Answers will vary.
2. Answers will vary.
3.
PART D
1. (Attached myogram)
2. (Labels for myogram)
3. Answers will vary.
4. Answers will vary.
5. Answers will vary.
6. Answers will vary.
72
LABORATORY EXERCISE 63
NERVE IMPULSE STIMULATION
Laboratory Report Answers
PART A
1. Potassium
2. Sodium
3. Potassium
4. Resting
5. Summation
6. Depolarization
7.
8.
9.
10.
11.
Refratory
Lipid
Schwann cells
Saltatory
Faster
PART B
1. Answers will vary.
2. Answers will vary.
3. Answers will vary.
4. Answers will vary.
73
LABORATORY EXERCISE 64
FACTORS AFFECTING THE CARDIAC CYCLE
Laboratory Report Answers
PART A
1. Pump the blood to the lungs and body cells
2. S-A node
3. Vagus
4. Acetylcholine
5. Norepinephrine (noradrenalin)
PART B
1. The atria contract as a unit followed by the
contraction of the ventricle (single in frogs)
2. (experimental results)
3. (experimental results)
6.
7.
8.
9.
10.
Medulla oblongata
Blood pressure (stretch)
Sympathetic (accelerator)
Increase
Potassium (K+)
4.
PART C
1. The force (amplitude) of the contractions normally
2.
increases as calcium ions help initiate muscle
contractions. There is danger of prolonged contraction.
74
Apparatus/Supplies/Equipment
Safety equipment (first aid kit, disposable latex and vinyl gloves,
safety glasses, laboratory coats, and disinfectant solution)
Compound light microscopes
Micrometer scale on microscope*
Oil immersion objective on compound microscope*
Stereomicroscopes (dissecting microscopes)
Lens paper
Microscope slides
Depression microscope slides
Coverslips
Transparent plastic millimeter ruler
Medicine dropper
Dissecting needle (needle probe)
Toothpicks (flat)
Single-edged razor blade*
Petri dish
Forceps
Thistle tube
Molasses (or Karo dark corn syrup)
Bone shears
Glass rod
Glass plate
Bunsen burner
Marking pen (washable)
Forceps (fine points)
Blunt metal probes
Watch that ticks
Tuning fork (128 or 256 cps)
Sterile cotton
Meterstick
Audiometer*
Ophtalmoscope*
Snellen eye chart
3 x 5 cards
Astigmatism chart
Pen flashlight
Ichikawas color plates or other colorblindness test
Small fish net
75
Laboratory thermometer
Cotton swabs (sterile; disposable)
Paper cups (small)
Swivel chair
Bright light
Sterile disposable blood lancets*
Slide staining rack and tray*
Heparinized microhematocrit capillary tube*
Sealing clay (or Critocaps)*
Microhematocrit centrifuge*
Microhematocrit reader*
Hemoglobinometer*
Hemolysis applicator*
Hemocytometer*
Unopette system (Becton Dickinson) for counting red
blood cells* (see Appendix 2 for a supplier of
Unopette systems)
Unopette system (Becton Dickenson) for counting white
blood cells* (see Appendix 2 for a supplier of
Unopette systems)
Hand counter (tally)*
ABO blood-typing kit
Anti-D serum*
Slide warming box (Rh blood-typing box or view box)*
Electrocardiograph (or other instrument for recording an
ECG)
Stethoscope
Cot or table
Electrode cream (paste)
Plate electrodes and cables
Glass tubing*
Rubber tubing*
Rubber stoppers, two-hole*
Pneumograph*
Disposable urine-collecting container
Urinometer cylinder
Urinometer hydrometer
pH test paper
Reagent strips (individual or combination) to test for the
presence of glucose, protein, ketones, bilirubin, and
hemoglobin/occult blood in the urine
Centrifuge
Centrifuge tubes
Normal and abnormal simulated urine specimens*
Paper cups
Vaseline*
Pennies (or other coins)
Dice
Syringe for U-100 insulin
Models/Skeletons
Dissectible human torso model with musculature
Animal cell
Animal mitosis
Human long bone, sectioned longitudinally
Articulated human skeleton
Disarticulated human skull (Beauchene)
Human skull, sagittal section
Fetal skull*
Disarticulated human skeleton
Vertebrae (cervical, thoracic, and lumbar examples)
Male and female pelves*
Synovial joints (shoulder, elbow, hip, and knee)
Skeletal muscle fiber model
Muscular model of the upper limb
Muscular model of the lower limb
Neuron
Spinal cord
Dissectible human brain
Dissectible ear
Dissectible eye
Dissectible human heart
Teeth, sectioned
Tooth model, sectioned
Larynx model
Thoracic organs model
Kidney model
Model of male reproductive system
Model of female reproductive system
Lung function model*
Models of human embryos
76
77
78
79
APPENDIX 3
Signature
Date
80
APPENDIX 4
Signature
Date
81