Code No: R05422301 Set No.

1
IV B.Tech II Semester Regular Examinations, Apr/May 2009
ANIMALS CELL SCIENCE AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Compare and contrast the principle and operation of:

(a) Bionocular microscope and

(b) Inverted microscope. [8+8]

2. Explain how you would:

(a) Prepare sterile media and reagents.

(b) Test the sterility of media. [8+8]

3. Define the principle of “Flow Cytometry” and comment on its potential applications
in:

(a) Monitoring growth.

(b) Cell cycle Analysis. [4+6+6]

4. Describe the principle of operation of flow cytophotometer (Fluorescence Activated

Cell Sorter) and its applications in cell separation and characterization. [16]

5. What is clonal yield and number of doubling of cells? Explain. [16]

6. Write in detail of the therapeutic applications of embryonic stem cell technology

by cell transplantation. [16]

7. Explain:

(a) Gas and nutrient exchange ,

(b) Structural integrity in organ cultures. [16]

8. Write the detailed explanation about the bone tissue engineering. [16]

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Code No: R05422301 Set No. 2
IV B.Tech II Semester Regular Examinations, Apr/May 2009
ANIMALS CELL SCIENCE AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Compare and contrast the principle and operation of:

(a) Bionocular microscope and

(b) Inverted microscope. [8+8]

2. Describe the essential features of media employed in:

(a) CO2 - gassed incubators.

(b) Non-gassed incubators. [8+8]

3. Describe the principle and method of operation of the following methods of cell
counting:

(a) Particle counter.

(b) Online cell concentration determination by ”Biomass Monitor”. [8+8]

4. Describe the principle of operation of flow cytophotometer (Fluorescence Activated

Cell Sorter) and its applications in cell separation and characterization. [16]

5. What is clonal yield and number of doubling of cells? Explain. [16]

6. Write in detail of the therapeutic applications of embryonic stem cell technology

by cell transplantation. [16]

7. Explain:

(a) Gas and nutrient exchange ,

(b) Structural integrity in organ cultures. [16]

8. What are the implications of tissue engineering in the bone metabolism? [16]

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Code No: R05422301 Set No. 3
IV B.Tech II Semester Regular Examinations, Apr/May 2009
ANIMALS CELL SCIENCE AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain how:

(a) You will prevent the spillages and

(b) Check and decontaminate, the spillages if any, in the cell culture laboratory.
[8+8]

2. Discuss the importance and functions of the following in the minimum essential
medium?
2+ 2+
(a) Ca and Mg
2+
(b) Fe
(c) NaCl
(d) Phosphates. [4×4]

3. List out any four methods of direct cell counting and comment on the relative
merits of each method. [16]

4. Define:

(a) Cell line.

(b) Cell strain.
(c) Passaging.
(d) Finite cell lines. [4×4]

5. What is plating efficiency? Explain the stimulation of plating efficiency. [16]

6. What are the methods of gene therapy on adult stem cells? [16]

7. Why cell should undergo apoptosis? What is its importance in cancer biology?[16]

8. How tissue engineering in oral and maxillofacial surgery? [16]

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Code No: R05422301 Set No. 4
IV B.Tech II Semester Regular Examinations, Apr/May 2009
ANIMALS CELL SCIENCE AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. List any four of the essential equipments, inside the cell culture laboratory and
mention their key features. [16]

2. Discuss the importance and functions of the following in the minimum essential
medium?
2+ 2+
(a) Ca and Mg
2+
(b) Fe
(c) NaCl
(d) Phosphates. [4×4]

3. Draw a typical cell growth and mark the following and explain their features:

(a) Lag Phase

(b) Exponential phase
(c) Stationary phase
(d) Decline or death phase. [4×4]

4. What are the important steps involved in primary cell culture? [16]

5. Explain the protocols involved in cell cloning. [16]

6. What are human embryonic stem cells? Explain its prospects for gene therapy.[16]

7. What is histotypic culture? Explain different techniques involved in this type of

culture. [16]

8. How tissue engineering can help in the process of invitro differentiation of mes-
enchymal cells? [16]

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