Annexure CD 01

FORMAT FOR COURSE CURRICULUM

L T P/S SW/F TOTAL

Course Title: W CREDIT Immunology and
Immunotechnology UNITS
3 - 1 - 4
Course Code: Credit
Units: 04
Course Objectives: Concepts of immunology and application of immunological techniques which can
help the students to understand the immunological aspects related to human health

Pre-requisites: Undergraduate in any branch of Science with Course in Life Sciences (for students with
No biology at School Level)

Student Learning Outcomes:

1. Basic Understanding of Immunology and its applications
2. Importance of Immunology in human health
3. Principles and hand-on experience of immunoassays

Course Contents/Syllabus:
Weightage (%)
Module I: Introduction to Immunology 15%
Types of immunity- active and passive, Innate and acquired immunity; Clonal nature of
Immune Response; Organization and structure of lymphoid organs; Cells of Immune System;
Hematopoeisis
Module II: Antigens and Antibodies 20%
Nature and Biology of antigens and super antigens; Antigen processing and presentation
Antibody structure and function generation of antibody diversity
Major Histocompatibility Complex
Module III: Mechanisms of Immune Responses 20%
Humoral and cellular immune responses; Complement System; Hypersensitivity; Regulation of
immune response; Immunological tolerance and autoimmunity
Module IV: Vaccines 20%
General Considerations; Live and Killed Vaccines; Adjuvant-Need, types; Other kinds of
Vaccines
Module V: Immunological Techniques 25%
Production of polyclonal antibodies; Hybridoma technology for production of monoclonal
antibodies; Agglutination; Immunodiffusion-types; ELISA-types, Immunoblotting;
 Immunofluorescence; Immunohistochemistry; Flow Cytometry

Pedagogy for Course Delivery: Interactive Classroom teaching using Audiovisual Aids
Lectures: 30 (including 2 Quizzes of 10 minutes each)
Class Test: 1
Total: 31

Lab/ Practicals details, if applicable:

List of Experiments:
1. Blood film preparation and identification of cells.
2. Bloog grouping by agglutination.
3. Isolation of serum from whole blood.
4. Microscopic examination of lymphoid organs
5. Radial Immunodiffusion test-SRID and DRID
6. WIDAL Test
7. ELISA-Dot and Sandwich
8. Immunohistochemistry
9. IgG purification by affinity chromatography

Assessment/ Examination Scheme:

Theory L/T (%) Lab/Practical/Studio (%) End Term Examination
60% 40% 70

Theory Assessment (L&T):

Continuous Assessment/Internal Assessment End Term
Examination
Components Class Quiz 1 Quiz 2 Home Attendance
(Drop down) Test Assignment

Weightage (%) 10 5 5 5 5 70

Lab/ Practical/ Studio Assessment:

Continuous Assessment/Internal Assessment

Components (Drop Class Test (Practical Practical Records Viva Attendance Practica
down) Based) Perform
Weightage (%) 33.2% 16.7% 33.3% 16.7% 6
Text:
1. Basic Immunology 4th Edition, by A.K. Abbas and A.H. Lichtman, Elsevier Publishers (2012)
2. Fundamentals of Immunology, Edited by W. Paul, Lippincot Williams and Wilkins Publishers (2008)
3. F.C. Hay, O.M.R. Westwood, Practical Immunology, 4th Edition-, Blackwell Publishing, 2002
4. S. Hockfield, S. Carlson, C. Evans, P. Levitt, J. Pintar, L. Silberstein, Selected Methods for Antibody
and Nucleic Acid probes, Volume1, Cold Spring Harbor Laboratory Press,1993.
5. Ed Harlow, David Lane, Antibodies Laboratory Manual, Cold Spring Harbor, Laboratory Press, 1988.
References:
1. Kuby Immunology 7th Edition, by Judy Owen, Jenni Punt, Sharon Stanford. WH Freeman Publishers
(2012).
Any other Study Material: None