TEST METHOD REAGENT USE; END PRODUCT NORMAL CONVERT S. I.

CLINICAL SIGNIFICANCE REFERENCE

SUBSTRATE MEASURE REFERENCE, ION METHOD
COLOR; CONVENTIONAL FACTOR
WAVELENGTH OR
TRADITIONAL
Trinders method -Glucose(Gl.) - Pink color - 70-110 mg/dL 0.05551 mmol/L Hyperglycemia Hexokinase
GLUCOSE enzyme reagent; - @510 nm Or Hypoglycemia
DETERMINATI ON -Gl. Buffer - 3.9-6.1 mmol/L To screen for, diagnose, and monitor
Folin-wu method high blood glucose (hyperglycemia)
Ortho-Toulidine by or low blood glucose
Dubowski (hypoglycemia), diabetes, and pre-
Nelsons Sumogyi diabetes
method
BLOOD UREA Modified Barthelot -Urea N-base - green color - 8-23 mg/dL 0.357 mmol/L Pre-ana Azotemia Diacetyl
NITROGEN Reagent - @630 nm Or Renal azotemia Monoxime by
-Urea N-zyme - 2.9-8.2 mmol/L Post-anal azotemia Fearon
reagent; To evaluate kidney function in a
-Urea N-color wide range or circumstances, to help
reagent diagnose kidney disease, and to
monitor patients with acute or
chronic kidney dysfunction or
failure.
Trinder-uricase Uri-color reagent; - Yellow-orange Male 0.05948 mmol/L Hyperucemia Phosphotungstic
BLOOD URIC ACID Uri-zyme buffer - @520 nm 4-8.5 mg/dL Lesh nyan syndrome Acid by caraway
Blauch and Koch -uricase - @290-300 nm 0.24-0.51 mmol/L Gout
method The uric acid blood test is used to
Catalase system -uricase - @410 nm detect high levels of this compound
Bittner method -copper - Deeply colored Female 2.7-7.3 in the blood in order to help
neocuprione substance mg/dL diagnose gout.
TPTZ (2,4,6- (2,4,6-tripyrid-5- - blue
tripyridyl-5- triazine) - @590 nm 0.16-0.43 mmol/L
triazine) by morin
PAP(4- -PAP - Red quinonemine
aminophenazon) -DHBS derivative
by Trinder - @480-550 nm
CREATANINE Folin-wu method Alkaline picrate - yellow color -0.6-1.2 mg/dl 88.40 mol/L GFR Jaffe reaction
solution - @520 nm -53-106 mol/L Hyperthryoidism
Jaffe Reaction -alkaline picrate - creatinine picrate Diabetic acidosis
solution orange red color Puerperium
To determine if your kidneys are
functioning normally and to monitor
treatment for kidney disease
Enzymati/Trinder Cholesterol - pink color -150-250 mg/dL 0.0256 mmol/L Hypercholestenemia: Liebermann-
CHOLESTEROL enzyme reagent; - @510 nm -3.88-6.47 mmol/L CAD burchard reagent
Cholesterol buffer CVD
Salkowski reaction -HAc - Hypocholesteronemia
-Fe(III) Choloestapolyene Hemolytic anemia
carbonium ion To screen for risk of developing
- red color heart disease
Liebermann- -acetic anhydrite - choloestapolyene
Burchard reagent -H2SO4 sulfonic acid
-green color
HDL-C Enzymatic/Trinder Cholesterol - Pink color Male .26 mmol/L The test for HDL cholesterol (HDL-C) Dextran sulfate -
enzyme reagent; - @520 nm 23-63 mg/dL is used along with other lipid tests to magnesium
Cholesterol buffer 6.76-16.38 mol/L screen for unhealthy levels of lipids
and to determine your risk of
Female developing heart disease.
35-75 mg/dl
8.58-19.5 mmol/l
TRIGLYCERIDE GPO-PAP Cholesterol - Pink color -10-190 mg/dL 0.0114 mmol/L Hypertriglycedemia Van Handell
METHOD-RANDOX enzyme reagent; - @500 nm -0.11-2.15 mmol/L Hypotriglycedemia Zilmersmith
Cholesterol buffer Blood tests for triglycerides are method
usually part of a lipid profile used to
identify the risk of developing heart
disease.
LDL-C Friedewald LDL-C Optimal .0259 mmol/L LDL cholesterol is used to predict Friedewald
method = T.choles.-HDL- <100 mg/dl risk of developing heart disease method
TG/5 mg/dl
<2.59 mmol/l
= T.choles.-HDL-
TG/2.175 mmol/L Near/above
 optimal
- 100-129mgdl
- 2.59-
3.34mmol
Borderline high
- 130-159mg/dl
-3.37-4.12mmol/l

Very high
- >189
- >4.9mol/l
TOTAL PROTEIN Biuret method Bromcresol blue - Purple color - 6-7.8 g/dL 10 g/L Hyperproteinemia Biuret method
- @545 nm - 60-78 g/L Hypoproteinemia
Total protein measurements can
reflect nutritional status may be
used to screen for and help diagnose
kidney disease, liver disease, and
many other conditions.
ALBUMIN Bromcresol green Bromcresol green - Lime green - 3.2-4.5 g/dL 10 g/L Hyperalbuminuria Bromcresol green
color Or Hypoalbuminuria
- @628 nm - 32-45 g/L To screen for a liver disorder or
kidney disease to evaluate
nutritional status, especially in
hospitalized patients, along with or
instead of a pre-albumin test.
BILIRUBIN Malloy and Evelyn Diazo reagent - Azobiliburin -Direct 17.10 mol/L Pre-hepatic Malloy and Evelyn
method Red violet - <0.3 mg/dL; Post-hepatic method
color or Hepatic jaundice
- @ 530 nm - <5 mol/
Bilirubin is measured to diagnose
-Indirect and/or monitor liver diseases, such
- 0.1-1 mg/dL as cirrhosis, or gallstones
or
- 2-17 mol/L

-Total
- 0.1-1.2
 mg/dL or
- 2-21 mol/L

Sources for reference values:

Mc Person, R. A., Pincus, M. R. 2011. Henrys Clinical Diagnosis and Management by Laboratory Methods. Saunders.

Burtis, C. A. & Ashwood, E. R. 2001. Tietz Fundamental of Clinical Chemistry. W. B. Saunders Company.