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Tiempo en
minutos
1
5
10
15
30
vel inicial
tiempo 0 1 5
concentracion 0 0.05 0.23
Concentracin de 1
f(x) = 0
glucos en mol/ml R = 0
0.8
0.05
0.6
0.23 Concentracion (mmol/ml)
0.38 0.4
0.52 0.2
f(x) = 0
1.03 0 R = 0.9
0 5
1
f(x) = 0.033026835x + 0.0390726125
0.8 R = 0.9973355794 Concentracin de glucos en mol/ml
Linear (Concentracin de glucos en
0.6 mol/ml)
mmol/ml)
0.4 vel inicial
Linear (vel inicial )
0.2
f(x) = 0.0457142857x + 0.0019047619
0 R = 0.9996745851
0 5 10 15 20 25 30 35
Tiempo (Min)
jssj
lineweaber-burk
t(min) Cs(mol/L) v(mol/L.min) 1/v
0 0.025 0.86 1.1627907
3 0.0227 0.79 1.26582278
6 0.0184 0.7 1.42857143
9 0.0135 0.65 1.53846154
12 0.0125 0.58 1.72413793
15 0.0073 0.46 2.17391304
20 0.0046 0.41 2.43902439
25 0.00204 0.2 5
30 0.00189
40 0.00185
lineweaber-burk
6
5
f(x) = 0.0081708463x + 0.9302592207
4 R = 0.9888050234
3 1/s
Axis Title
Linear ( 1/s)
2
0
0 100 200 300 400 500 600
Axis Title
lineweaber-burk Eadie Hofstee Lagmuir
1/s v v/s s/v s
40 0.86 34.4 0.02906977 0.025
44.0528634 0.79 34.8017621 0.02873418 0.0227
54.3478261 0.7 38.0434783 0.02628571 0.0184
74.0740741 0.65 48.1481481 0.02076923 0.0135
80 0.58 46.4 0.02155172 0.0125
136.986301 0.46 63.0136986 0.01586957 0.0073
217.391304 0.41 89.1304348 0.01121951 0.0046
490.196078 0.2 98.0392157 0.0102 0.00204
0.00189
0.00185
Eadie Hofstee
1
0.8
f(x) = - 0.0082841924x + 1.0492827829
0.6 R = 0.8965302778
1/s v/s
Linear ( 1/s) Axis Title
0.4 Linear ( v/s)
0.2
0
30 40 50 60 70 80 90 100 110
Axis Title
0 0.025 0.86
3 0.0227 0.79
6 0.0184 0.7
9 0.0135 0.65
12 0.0125 0.58
15 0.0073 0.46
20 0.0046 0.41
25 0.00204 0.2
30 0.00189
40 0.00185
Lagmuir
0.035
0.03
f(x) = 0.8786159528x + 0.008816407
0.025 R = 0.9718398317
0.02
v/s s
Linear ( v/s) Axis Title 0.015 Linear ( s)
0.01
0.005
0
0 0.005 0.01 0.015 0.02 0.025 0.03
Axis Title
s
Linear ( s)
jssj
lineweaber-burk
S(mg/ml) r 1/v 1/s
12.56 101 0.00990099 0.07961783
11.24 98.2 0.01018330 0.08896797
9 92.4 0.01082251 0.11111111
8.12 90 0.01111111 0.12315271
6.33 82.7 0.01209190 0.15797788
5.61 79.1 0.01264223 0.17825312
4.28 70.9 0.01410437 0.23364486
3.56 65 0.01538462 0.28089888
2.34 51.7 0.01934236 0.42735043
1 28.8 0.03472222 1.00000000
lineweaber-burk
0.04000000
0.03500000
f(x) = 0.0269271817x + 0.0078114509
0.03000000
R = 0.999986653
0.02500000
0.02000000 1/s
Axis Title
0.01500000 Linear ( 1/s)
0.01000000
0.00500000
0.00000000
0.00000000 1.00000000 2.00000000
Axis Title
Eadie Hofstee Lagmuir
v v/s s/v s
101 8.04140127 0.12435644 12.56
98.2 8.7366548 0.11446029 11.24
92.4 10.2666667 0.0974026 9
90 11.0837438 0.09022222 8.12
82.7 13.0647709 0.07654172 6.33
79.1 14.0998217 0.07092288 5.61
70.9 16.5654206 0.06036671 4.28
65 18.258427 0.05476923 3.56
51.7 22.0940171 0.04526112 2.34
28.8 28.8 0.03472222 1
Eadie Hofstee
120
100
f(x) = - 3.4632382219x + 128.2786803538
80 R = 0.9998307023
1/s 60 v/s
Linear ( 1/s)
Axis Title
Linear ( v/s)
40
20
0
5 10 15 20 25 30
Axis Title
Lagmuir
0.14
0.12
f(x) = 0.0077677898x + 0.027157617
0.1 R = 0.9999520256
0.08
v/s s
Axis Title 0.06
Linear ( v/s) Linear ( s)
0.04
0.02
0
0 2 4 6 8 10 12 14
Axis Title
s
Linear ( s)
jssj
Sin inhibicion
Metodo grafico
S V
3.7 10
13 30
39 63
79 87
230 116
400 122
a.
140
120
100
80
Axis Title 60
40
20
0
0 50 100 150 200 250 300 350 400 450
Axis Title
Vmax 120
Km 40
B.
GRAFICA. LINEWEAVER-BURK
0.2
f(x)
f(x)==0.7165559777x
1.7564195672x++0.0038957836
0.0043234238
0.18 R
R==0.9963064803
0.9968335177
0.16
0.14
0.12
0.1
1/V f(x) = 0.3433682403x + 0.0071334618
R = 0.9999849754
0.08
0.06
0.04
0.02
0
-0.02 0.03 0.08 0.13 0.18 0.23 0.28
1/S
0.06
0.04
0.02
0
-0.02 0.03 0.08 0.13 0.18 0.23 0.28
1/S
inhibicion 1 inhibicion 2
acetofenona 3,7 x10-4M acetofenona 1,58X10-3M
V (nmol/min) V (nmol/min)
5
20
45 20
70 40
102 80
122 110
lineweaber-burk
0.12
0.1
f(x) = 0.3433682403x + 0.0071334618
0.08 R = 0.9999849754
0.06
Axis Title
0.04
0.02
0
300 350 400 450 0 0.05 0.1 0.15 0.2 0.25 0.3
Axis Title
Vmax 140.84507
Km 48.366197
inhibicion 1 inhibicion 2
acetofenona 3,7 x10-4M acetofenona 1,58X10-3M
V (nmol/min) V (nmol/min)
5
20
45 20
70 40
102 80
122 110
inhibicion 1 inhibicion 2
acetofenona 3,7 x10-4M acetofenona 1,58X10-3M
1/V 1/V
0.2
0.05
0.0222222222 0.05
0.0142857143 0.025
0.0098039216 0.0125
0.0081967213 0.0090909091
Eadie Hofstee
140
120
f(x) = - 47.1305080736x + 138.3874253584
100 R = 0.9992324467
80
1/v
Linear ( 1/v)
Axis Title 60
40
20
0
25 0.3 0 0.5 1 1.5 2 2.5
Axis Title
Vmax 138.39
Km 46.131
v 2
7317065x + 0.3327524172
759271
s/v
Linear ( s/v)
Axis Title
jssj
Graficas Linewaver-burk
sin inibicion
0.04
0.035
f(x) = 0.0269271817x + 0.0078114509
0.03
R = 0.999986653
0.025
0.02
Axis Title
0.015
0.01
0.005
0
0 0.2 0.4 0.6 0.8 1 1.2
Axis Title
Axis Title
0.015
0.01
0.005
0
0 0.2 0.4 0.6 0.8 1 1.2
Axis Title
Vmax 128.21
Km 3.45
Tipo de inhibicion
0.04
0.03
1/V 0.02
0.01
1.21430643318377E-017
0 0.2 0.4 0.6 0.8 1 1.2
-0.01
1/S
Inhibicion 2
1/s 1/v
0.03003003 0.00862069
0.05 0.00917431
0.07751938 0.00980392
0.1 0.01169591
0.1650165 0.01398601
0.27472527 0.01798561
0.35460993 0.0210084
0.54347826 0.02808989
Inhibicion
0.035
0.03
f(x) = 0.0325340303x + 0.0097715194
0.025
R = 0.9999421889
0.02
Axis Title 0.015
0.01
0.005
0
1.2 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7
Axis Title
Axis Title 0.015
0.01
0.005
0
1.2 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7
Axis Title
Vmax 102.04
Km 3.32
con maltosa
0.05
0.04
0.03
1/V 0.02
0.01
1.21430643318377E-017
.8 1 1.2 -0.1 0.1 0.3 0.5 0.7 0.9 1.1
-0.01
1/S
0.015
Axis Title
0.01
0.005
0
0.6 0.7 0 0.1 0.2 0.3 0.4 0.5 0.6
Axis Title
Axis Title
0.01
0.005
0
0.6 0.7 0 0.1 0.2 0.3 0.4 0.5 0.6
Axis Title
Vmax 135.14
Km 5.16
maltosa
1/S