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Report of Fundamental of Microbiology Lab

Gram Stain and Simple Stain


Supporting Lecturer: Ajarn. Zubaidah

International Biology Education 2015


Diqna Nur Annisa (405952045)
Ratna Dyah Hartanti (405952046)
Lintang Faradhiba (405952047)
Mala Antika Suri (405952048)
Zahra Noor Ayuna (405952049)

TRANSFER CREDIT PROGRAM


DEPARTMENT OF BIOLOGY EDUCATION
FACULTY OF MATHEMATIC AND NATURAL SCIENCE
YOGYAKARTA STATE UNIVERSITY
2017
Apendix

Bacillus sp. Simple Stain Bacillus sp. gram positif (Gram Stain)

Stapilococcus aureus gram positif (Gram Stain) Bacillus sp. (Simple Stain)

E. coli (Gram Stain) Streptobacil aureus (Gram Stain)


S. aureus (Simple Stain) E. coli. gram (Simple Stain)
Table of Result

No Bacteria Result (Color)


Streptococcus aureus blue violet
Streptobacil aureus blue violet
1 Gram Stain
Bacillus sp blue violet
E, coli red
Bacillus sp bacilli or rod-shaped
bacteria
2 Simple Stain E, coli bacilli or rod-shaped
bacteria
Staphylococcus aureus cocci or spherical

Gram Stain

The Gram stain procedure uses 3 different stains. These are crystal violet, Gram's iodine, and
safranin. The cells are first stained with crystal violet, then Gram's iodine. Following a rinse in
alcohol, to de-colorize the cells, the cells are then stained with safranin.

From the result, show that Streptococcus aureus, Streptobacil aureus and Bacillus sp. get the blue
violet color stain. It is show that Streptococcus aureus, Streptobacil aureus and Bacillus sp. are
Gram-positive bacteria. But E. coli get red color stain, its show that E. coli is gram-negative
bacteria. Bacteria take up the Gram stain differently because they differ in cell wall composition.
Gram-positive bacteria have a thick cell wall layer (peptidogycan). Alcohol does not readily
penetrate to decolorize the cell wall of the previously applied crystal violet stain. Gram-negative
cells have a thinner cell wall (peptidogycan) through which the alcohol readily penetrates. The
crystal violet is removed from these cell walls that are then stained with the safranin counterstain.

Simple Stain

A simple stain consists of a solution of a single dye. Some of the most commonly used dyes are
methylene blue, basic fuchsin, and crystal violet. Simple stains allow one to distinguish the shape
(morphology) of the bacteria.
From the result, show that E. coli and Bacillus sp. are bacilli or rod-shaped bacteria. Based on
picture that we have observed, show that Bacillus sp. connect together forming a chains. E. coli
show bacilli or rod-shaped occur singularly. Bacilli very greatly in length and diameter.
Staphylococcus aureus show that it is cocci or spherical bacteria in clusters (as in Staphylococcus
aureus).

Conclusion
Based on result, we can conclude that Streptococcus aureus, Streptobacil aureus and
Bacillus sp. are Gram-positive bacteria that show with blue violet color. And E, coli is Gram-
negative bacteria that show with red color that use by Gram Stain test. In Simple stain, show that
Bacillus sp. and E.coli are bacilli or rod-shaped bacteria, and Staphylococcus aureusis cocci oe
spherical shaped bacteria.

References

http://www.waksman-foundation.org/labs/rochester/grmstain.htm
https://quizlet.com/19072763/simple-stain-and-gram-stain-flash-cards/

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