HARLECO Dyes & Stains

Microscopy products to
support your success
For over 90 years, Harleco brand dyes and stains
have offered our customers outstanding contrast,
color, and clarity in their sample specimens. Harleco
stain solutions offer not only the classical formula-
tions, but also many new and improved modifications
developed from years of experience. We use Certified
Biological Stains and cGMP manufacturing for all
Harleco products to provide you with the highest
quality stain solutions. In addition, we offer a MIDAS
III Automated Stainer and a complete line of products
for sample preparation.
Hematology
Reagents to identify diseases of the blood and blood forming organs

The Harleco product line offers ready to use staining solutions for blood and bone marrow samples that
yield intense and reproducible results. Reproducibility can be enhanced by the use of phosphate buffer
solution, which stabilizes diluted staining solutions. We offer buffers with different pH levels, so that you
may obtain optimal staining results. For higher slide staining throughput or for preparing urgent lab
samples, Hemacolor, the fast staining kit, can give very clean results in less than one minute.

HARLECO Giemsa Stain Solution, Azure B 619-71:500mL

HARLECO Giemsa Stain Solution, Modified Azure Blend 620-75:1L
HARLECO Giemsa Stain Solution, Original Azure Blend 620G-75:1L
Giemsa Stain Solution R03055-74:500mL; R03055-76:1L; R03055-82:4L
HARLECO Jenner Stain Solution 642-75:1L
HARLECO May-Grunwald Solution 660-75:1L; 660-85: 4L
HARLECO Wright Stain Solution 740-75:1L; 740-85:4L; 740-86:10L
HARLECO Wright Giemsa Solution, APHA 742-75:1L; 742-86:10L
HARLECO Wright Giemsa Solution, Fucillo 64571-95:500mL; 64571-75:1L; 64571-85:4L
HARLECO Wright Giemsa Stain Pack 65042-93:1PC
HARLECO Wright Stain Pack 65043-93:1PC
Leishman Stain Solution R03087-76:1L
HARLECO Hemacolor Stain Set 65044-93:1PAC
HARLECO Hemacolor Solution I 65044A-85:4L
HARLECO Hemacolor Solution II 65044B-85:4L
HARLECO Hemacolor Solution III 65044C-85:4L
HARLECO Buffer, Phosphate pH 6.4 1217-75:1L; 1217-85:4L
HARLECO Buffer, Phosphate pH 6.8 1218-75:1L; 1218-85:4L
HARLECO Buffer, Phosphate pH 7.0 1219-75:1L; 1219-85:4L

Wright staining-blood smear

Cytology
Reagents for brilliant and highly reproducible sensitive results

To stain gynecological and clinical specimens, use standard Papanicolaou sample staining with hematoxylin
Gills solution. In addition, you can rely on our high quality alcohol and the xylene substitute, Neo-Clear,
to further improve your results.

HARLECO Hematoxylin, Gill I 65065-85:4L

HARLECO Hematoxylin, Gill II 65066-85:4L
HARLECO Hematoxylin, Gill III 65067-85:4L
HARLECO Hematoxylin, Harris Alum, Hg Free 638A-71:500mL; 638A-85: 4L
HARLECO EA Solution, Gill (with Eosin and Light Green) 65068-85:4L
HARLECO Orange G Solution, Gill 65069-85:4L
HARLECO Papanicolaou, OG-6 7052X-75:1L; 7052X-85:4L; 7052X-86:10L
HARLECO Papanicolaou, EA-65 7054X-85:4L; 7054X-86:10L
HARLECO Papanicolaou, EA-36 7058X-75:1L; 7058X-85:4L
HARLECO Papanicolaou, EA-50 7062X-75:1L; 7062X-85:4
HARLECO Blueing Reagent 65354-85:4L
HARLECO Acid Alcohol, 70% (3% HCl) 752X-75:1L
HARLECO Acid Alcohol, 95% (5% HCl) 753X-75:1L
HARLECO Alcohol, 100% 65347-85:4L
HARLECO Alcohol, 95% 65348-85:4L
HARLECO Alcohol, 80% 65349-85:4L
HARLECO Alcohol, 70% 65350-85:4L
Neo-Clear Xylene Substitute 65351-85:4L

Papanicolaou stain gynecological sample

Histology
Reagents for brilliant and stable results from the first to the last step in the process

Precise specimen preparation is a necessity to get good slides for diagnostic work. We offer all of the
products needed for preparing histology samples, including top quality fixing media and high purity
solvents. The Harleco stain results are color stable, reproducible and offer sufficient sensitivity to reveal
all structural details.

HARLECO Eosin Y Solution, Alcoholic Saturated 586X-75:1L

HARLECO Eosin Y Solution, Aqueous, 1% PDC 588X-75:1L
HARLECO Eosin Y Solution, Aqueous, 1% PDC 592-75:1L
Eosin Stain Solution, 5% R03040-74:500mL
HARLECO Schiff Reagent 2818-71:500mL
HARLECO Schiff Reagent, Hotchkiss/McManus 6073-71:500mL
HARLECO Gomori Trichrome 623-71: 500mL
Formalin 10% W/V, Neutral Buffered R04586-76:1L; R04586-82:4L; R04586-86:20L; R04586-90:200L
HARLECO Formalin, Neutral Buffered 10% 65346-85:4L; 65346-88:20L
Formalin 10%, Neutral Buffered R04586B-82:4L
Formalin 20%, Phosphate Buffered R4587-01:1CV; R4587-86:20L
Formalin 10%, Acetate Buffered R04588-86:20L
Formalin Neutral 10%, Carbonate Buffered R03379-82:4L; R03379-86:20L
HARLECO Formalin, Carsons 65115-88:20L
SAF Fixative (Acetate Buffered Formalin) R04650-90:200L
SAF Fixative with Triton (Acetate Buffered Formalin) R04561-90:200L
Formaldehyde Kit Concentrates to make NBF R04669-01:1PC
HARLECO Bouin Fixation Fluid 7831-85:4L; 7831-88:20L
HARLECO Fixative, Haupts 785-71:500mL
Albumin-Glycerol Fixative R03203-70:100mL; R03203-74:500mL
HARLECO Blueing Reagent 65354-85:4L
Mollifex Tissue Softener 65039-71:500mL
HARLECO Acid Alcohol, 70% (3% HCl) 752X-75:1L
HARLECO Acid Alcohol, 95% (5% HCl) 753X-75:1L
HARLECO Alcohol, 100% 65347-85:4L
HARLECO Alcohol, 95% 65348-85:4L
HARLECO Alcohol, 80% 65349-85:4L
HARLECO Alcohol, 70% 65350-85:4L
Neo-Clear Xylene Substitute 65351-85:4L
HARLECO Krystalon Mounting Medium 64969-95:300mL; 64969-71:500mL

H + E Stain - Liver tissue stained with

eosin Y solution
Microbiology
Reagents for standard stains and reliable results with manual or automated staining

Harleco bacteriological standard stains for Gram and TB detection offer easy application and reproducible,
stable results. The staining solutions can be used for manual as well as for automated staining procedures
in the Midas III automated stainer.

HARLECO Gram Staining Set, 4 x 500mL 65092-93:1PAC

HARLECO Crystal Violet, Gram Solution 65092A-95:1PAC; 65092A-85:4L
HARLECO Safranin Solution, Gram 65092B-95:1PAC; 65092B-85:4L
HARLECO Iodine Solution, Gram (Stabilized) 65092D-95:1PAC; 65092D-85:4L
HARLECO Decolorizer, Gram Solution 65092E-95:1PAC; 65092E-85:4L
HARLECO Iodine Lugol's 624-71:500mL
Safranin Solution, 1% R03131-74:500mL
HARLECO Fuchsin, Basic Solution 6039X-75:1L
HARLECO Fuchsin, Carbol Solution, Ziehl-Neelson 6051X-75:1L
HARLECO Fuchsin, Carbol Solution, Kinyoun 6033X-75:1L
Methylene Blue Stain Solution, Loeffler`s R03101-74:500mL
HARLECO Methylene Blue, Loeffler's 687X-75:1L
Nigrosine Solution, Aqueous R03418-70:100mL
Lactophenol Solution R03266-74:500mL
Lactophenol, Cotton Blue R03465-70:100mL

Gram stain - mixture of E. coli and cereus bacillus culture

Midas III Automated Slide Stainer

The Midas III Automated Stainer is designed to work with Harleco stains and is flexible enough to adapt to your
current staining protocols. With a small footprint to allow use on the smallest benchtops, the Midas III
Automated Stainer yields staining results that are more reproducible than manual staining.

The six station unit holds either 20 or 30 slides. Up to 10 programs may be introduced, with each program
comprised of as many as 29 steps. Save up to 50% on stain consumption as compared versus flood or spray
techniques.

Midas III Stainer 64000H-94: 1 pc

20 position slide carrier each 64052-94: 1 pc
30 position slide carrier each 64052E-94: 1 pc
Water Bath each 64000WB-94: 1 pc
Water Bath Filters Package of two 64000WBF-95: 1 pc
Vessels & Lids White Package of Eight 64054W-95: 1 pc
Operators Manual 64000OP-94: 1 pc
Service Manual 64000SM-94: 1 pc
Multi-tergedetergent 65058-85: 4L
Harleco Water, distilled ASTM type II 6442-88:4L; 6442-88: 20L

Midas III Automated Slide Stainer

For more information, or to order product
Contact us at:
Phone : 1-800-645-5476
Email: nacustomerservice@merckgroup.com

EMD Millipore
290 Concord Road
Billerica, MA 01821

www.emdchemicals.com
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