Disinfectant Generation System

Preparation Of Sterisol
Sterisol super oxidized water is a high level disinfectant, which is produced on site using
Steri-gen disinfectant generation system.

Pre-installation requirements for demo installation:

1. A well ventilated area around 40-50 sq. feet.

2. Water point for regular supply of potable water (with low TDS).
3. Power connection 220 V, 15 Amp.
4. Drainage outlet.
5. Plastic containers for collection of Sterisol.

Sterisol generation parameters:

1. Sterisol should be produced using Sterisol-C electrolyte solution supplied by Faith

Biotech Pvt. Ltd.. Sterisol-C is a specially prepared electrolyte solution with the
required concentration of various salts at pre determined pH.
2. The current on the ammeter at the machine panel should be maintained between 8-
10 Amp.
3. The catholyte tap should be in closed position.

WARNING:

Steri-gen system should be operated only by trained personnel.

 PROTOCOL – Application of Sterisol
1. AERIAL FUMIGATION:

Aerial Disinfection is to be carried out with Fogging machine with a droplet

size of 5-10 micron to ensure a safe and sterile environment.

CONCENTRATION: Undiluted
APPLICATION PROCEDURE:

1. Clean the room thoroughly before fumigation.

2. Do the Surface / Walls Disinfection with Sterisol using a clean mop
dipped in Sterisol (As per protocol for house keeping surface
disinfection).
3. Start Fumigation.
4. AHU should be kept on for the first 10 minutes of fumigation. Switch
off the AHU after 10 minutes.
5. Fumigation should be done for 25 minutes after switching off the
AHU.
6. After fumigation the room is to be closed for 45 minutes.
7. The AHU can now be switched on and the room is ready for use.

Notes :
• For optimum result the droplet size should be between 5-10 micron and
20 ml /m3 (560 ml /1000 CFT) volume to be used for fogging.
• The fumigation machine to be mounted 2 feet from the floor and it
should be moved to various areas of the room.
• The angle of the Pump to be kept at 45 degrees.
• Ensure that a proper mist is created in the area so that Sterisol is
suspended in the atmosphere, thereby giving complete aerial
Disinfection.
• After every fumigation, add plain water to the fumigation machine and
run it for 2 min. This will help in keeping the fogger clean.
• Supply correct voltage to the machine as recommended.
• Thoroughly wipe / clean the outer body and the tank of Fogger with
plane cloth after each fumigation.
 EVALUATION PROCEDURE:

Collection of Pre-treatment samples:

1. Mark at least 3 areas each on the walls, floor and surfaces.
2. Collect at least 1 sample from each of these areas after Step – 1.
3. Collect at least 3 Air Samples after Step – 1.
Collection of Post-treatment samples:
4. Collect at least 1 sample from the previously marked areas after
Step – 6.
5. Collect at least 3 Air Samples after Step – 6.

2. MEDICAL EQUIPMENT SURFACE DISINFECTION (e.g. hemodialysis

machines, x-ray machines, instrument carts, Dental Units etc.)

CONCENTRATION: Diluted in a ratio of 1:2 with normal water (1 part

sterisol and 1 part of water).

APPLICATION PROCEDURE:

1. Clean the surface thoroughly.

2. Remove dirt, stain, blood or other body fluids, using mild detergent
and/or enzymatic cleaner.
3. Add 1 sachet of Corrosion Inhibitor (supplied by FBPL) in 5 Lit. of
SteriSol.
4. Use soft cloth dipped in Sterisol for disinfecting.
5. Change the Sterisol Solution, when it becomes dirty.
6. Repeat every day or whenever there is spillage.
NOTE: Kindly dip the mop/cloth in Sterisol for 5 minutes prior to use.

EVALUATION PROCEDURE:

Collection of Pre-treatment samples:

6. Determine at least 3 areas each on Surface of the equipment.
7. Collect at least 1 sample from each of these areas after Step – 2.
Collection of Post-treatment samples:
8. Collect at least 1 sample from the previously marked areas 5
minutes after Step – 3.
3. HOUSE KEEPING SURFACES (e.g., walls, tabletops, shelves, racks
etc.).

CONCENTRATION: Diluted in a ratio of 1:2 with normal water (1 part

sterisol and 1 part of water)
.
APPLICATION PROCEDURE:
1. Use clean, washed Mopping Head (Cloth).
2. Before starting the mopping, dip the mop for 5 minutes in Sterisol.
3. Mop all surfaces.
4. Change the Sterisol Solution, when it becomes dirty.
5. Sterisol mopping to be done 1-2 times a day.

EVALUATION PROCEDURE:

Collection of Pre-treatment samples:

1. Determine at least 3 areas each on Surface.
2. Collect at least 1 sample from each of these areas before
mopping.
Collection of Post-treatment samples:
1. Collect at least 1 sample from the previously marked areas 5
minutes after Step – 3.

4. FLOOR MOPPING NON SENSITIVE AREAS (eg. Wards, corridors, etc.)

CONCENTRATION: Diluted in a ratio of 1:8 with normal water (1 part

sterisol and 7 parts water)

APPLICATION PROCEDURE:
1. Use clean, washed Mopping Head (Cloth).
2. Before starting the mopping, dip the mop for 5 minutes in Sterisol.
3. Mop the floors as per house keeping protocols.
4. Change the Sterisol Solution, when it becomes dirty.
5. Sterisol mopping to be done as per house keeping protocols.

EVALUATION PROCEDURE:

Collection of Pre-treatment samples:

3. Determine at least 3 areas each on Surface.
4. Collect at least 1 sample from each of these areas before
mopping.
 Collection of Post-treatment samples:
2. Collect at least 1 sample from the previously marked areas 5
minutes after Step – 3.

5. FLOOR MOPPING SENSITIVE AREAS (eg. ICUs, ORs, etc.)

CONCENTRATION: Diluted in a ratio of 1:3 with normal water (1 part

sterisol and 2 parts water)

APPLICATION PROCEDURE:
1. Use clean, washed Mopping Head (Cloth).
2. Before starting the mopping, dip the mop for 5 minutes in Sterisol.
3. Mop the floors as per house keeping protocols.
4. Change the Sterisol Solution, when it becomes dirty.
5. Sterisol mopping to be done as per hospital protocols ( 2 times a
day).
EVALUATION PROCEDURE:

Collection of Pre-treatment samples:

5. Determine at least 3 areas each on Surface.
6. Collect at least 1 sample from each of these areas before
mopping.
Collection of Post-treatment samples:
3. Collect at least 1 sample from the previously marked areas 5
minutes after Step – 3.

6. HAND/ FEET DISINFECTION:

CONCENTRATION: Undiluted
APPLICATION PROCEDURE:

Non Surgical
1. Take 5 ml Sterisol in Hand, using a dispenser
2. Rub Sterisol on your hands thoroughly, till it becomes dry (1-2 Min)

Surgical
1. Clean/scrub hands thoroughly, using standard technique
2. Take 10 ml Sterisol in Hand
3. Rub Sterisol on your hands thoroughly, till it becomes dry (3 min)
 4. Alternatively, dip your hand in Sterisol Bath for 3 minutes. Use Sterile
Towel to Dry.

EVALUATION PROCEDURE:

Collection of Pre-treatment samples:

1. Collect at least 3 samples before application of Sterisol.
Collection of Post-treatment samples:
2. Collect at least 3 samples after drying of hands.

7. VEGETABLE/FRUIT WASHING

CONCENTRATION: Diluted in a ratio of 1:10 (1 part Sterisol and 9

parts of water)

APPLICATION PROCEDURE:

1. Clean the fruits/vegetables manually to remove any mud/dust etc.

2. Wash the fruits/vegetables thoroughly using normal tap water.
3. Dip the fruits/vegetables in diluted Sterisol for 5 minutes and use for
cooking/making salads etc.

EVALUATION PROCEDURE:

Collection of Pre-treatment samples:

1. Collect at least 3 samples from each vegetable surface after Step
– 2.
Collection of Post-treatment samples:
2. Collect at least 3 samples after Step – 3.

8. INSTRUMENT DISINFECTION

CONCENTRATION: Diluted in a ratio of 1:2 (1 part sterisol and 1 part

of water)
APPLICATION PROCEDURE:
1. Mechanically clean the surfaces and lumens thoroughly.
2. Remove dirt, stain, blood or other body fluids, using mild detergent
and/or enzymatic cleaner.
3. Add 1 sachet of Corrosion Inhibitor (supplied by FBPL) in 5 Lit.
of SteriSol. This is required to reduce corrosive activity.
4. Flush the lumens of instruments if any (using syringe) with Sterisol
thoroughly. During the last flush leave Sterisol in the lumen.
 5. Dip the instruments for 10 minutes in Sterisol to achieve High Level
Disinfection.
Do not leave instruments in Sterisol for more than 30 minutes, as
corrosion might occur in inferior grade steel.

EVALUATION PROCEDURE:

Collection of Pre-treatment samples:

1. Collect at least 3 samples from each instrument after Step – 2.
Collection of Post-treatment samples:
2. Collect at least 3 samples after Step – 4.

9. BIOFILM REMOVAL

CONCENTRATION: Diluted in a ratio of 1:2 (1 part sterisol and 1 part

of water)

APPLICATION PROCEDURE:
1. Mechanically clean the surfaces and lumens thoroughly.
2. Remove dirt, stain, blood or other body fluids, using mild detergent or
enzymatic cleaner.
3. Add 1 sachet of Corrosion Inhibitor (supplied by FBPL) in 5 Lit.
of SteriSol. This is required to reduce corrosive activity.
4. Flush the lumens of instruments if any (using syringe) with Sterisol
thoroughly. During the last flush leave Sterisol in the lumen.
5. For the removal of Biofilm, dip in Sterisol (Surface and lumen) for 20
min.
6. Dry the instrument in clean area.
7. Repeat Step 4 for stubborn Biofilm.
For maintaining Biofilm Free Surfaces, follow Instrument Disinfection
Guidelines.

Note: Biofilm removal may take more than one treatment cycle.
Confirm Biofilm removal using standard microbiology practices.

Collection of Pre-treatment samples:

1. Determine at least 3 areas each on Surface & lumen of the
instrument.
2. Collect at least 3 samples from each of these areas after Step – 2.
Collection of Post-treatment samples:
1. Collect at least 3 samples from the same areas after Step – 4.
Application Dilution Protocol
Aerial Fumigation Undiluted Start Fumigation
5-10 micron droplet size Keep AHU on for 10 min
20ml/ cubic meter (560ml/ Fumigate for another 25
1000 CFT) mins
Keep the room closed
for 45 minutes
Switch on the AHU
Medical Equipment 1 : 2 (1 part SteriSol mixed Clean Surface
Surface Disinfection with 1 part water) Dip the cloth for 5
minutes, prior to use.
(Hemodylasis Add 1 sachet of Corrosion Spray Sterisol using a
machine, X-Ray Inhibitor (supplied by FBPL) in spray bottle and wipe
Machine, Instrument 5 Lit. of SteriSol. witrh a soft cloth or mop
Carts, Dental Units with a soft cloth dipped
etc.) in SteriSol.
Change SteriSol when
the water becomes dirty.
House Keeping 1 : 5 (1 part SteriSol mixed Use Clean, washed
Surfaces with 4 parts water) mopping cloth
Dip the cloth for 5
(Walls, table tops, minutes, prior to use.
shelves, racks etc.) Spray Sterisol using a
spray bottle and wipe
witrh a soft cloth or mop
with a soft cloth dipped
in SteriSol.
Change SteriSol when
the water becomes dirty.
Floor Mopping Non 1 : 8 (1 part SteriSol mixed Use Clean, washed
Sensitive Areas with 7 parts water) mopping cloth
(Wards & Corridors Dip the cloth for 5
etc.) minutes, prior to use.
Mop the floors as per
housekeeping protocol
Application Dilution Protocol
Floor Mopping 1 : 3 (1 part SteriSol mixed Use Clean, washed
Sensitive Areas with 2 parts water) mopping cloth
(ICU, ORs, Burns Dip the cloth for 5
Ward, Labour minutes, prior to use.
Room, Transplant Mop the floors as per
Unit etc.) housekeeping protocol

Hand/ Feet Undiluted Take 5 ml Sterisol in

disinfection hand, using a dispenser
Rub Sterisol on your
Non Surgical hands thoroughly, till it
dries (1-2 min)
Hand/ Feet Undiluted Clean/ Scrub Hands
disinfection using standard
technique.
Surgical Take 10 ml Sterisol in
hand, using a dispenser
Rub Sterisol on your
hands thoroughly, till it
dries (3 min)

Alternatively dip your

hands in SteriSol bath
for 3 min. Use Sterile
Towel to dry.
Vegetable/ Fruit 1 : 10 (1 part SteriSol and 9 Clean to remove mud/
Washing parts of water) dust etc.
Wash thoroughly using
normal tap water.
Dip in diluted sterisol for
5 minutes
Application Dilution Protocol
Instrument 1 : 2 dilution (1 Mechanically clean, surface and
Disinfection part SteriSol & 1 lumen
part water) Remove dirt, stain, blood or other
body fluids using detergent/ enzymatic
Add 1 sachet of cleaner.
Corrosion Flush the lumen with SteriSol using
Inhibitor (supplied syringe
by FBPL) in 5 Lit. During the lasy flush leave SteriSol in
of SteriSol. the lumen.
Dip instrument in Sterisol for 10 min.
Do not leave the instrument for more
than 30 min in SteriSol.
BioFilm 1 : 2 dilution (1 Mechanically clean, surface and
Removal part SteriSol & 1 lumen
part water) Remove dirt, stain, blood or other
body fluids using detergent/ enzymatic
Add 1 sachet of cleaner.
Corrosion Flush the lumen with SteriSol using
Inhibitor (supplied syringe
by FBPL) in 5 Lit. During the last flush leave SteriSol in
of SteriSol. the lumen.
For removal of biofilm, dip in Sterisol
(Surface & Lumen) for 20 min.
Dry the instrument in clean area.

For any questions, contact

Faith Innovations
E Mail : faithinnovations@gmail.com