Lampiran

Rumus :

m = 5 – a (1)

b= ∑xy – 1/n (∑x. ∑y) (2)

∑x2 – 1/n (∑x)2

a = 1/n (∑y – b . ∑x) (3)

Daphnia sp

(BENZENA)

b = ∑xy – 1/n (∑x. ∑y)

∑x2 – 1/n (∑x)2

= 275,0637461 – 1/12 (37,45561707)( 88,16)

118,0955403 – 1/12(37,45561707)2
= -0,58

a = 1/n (∑y – b . ∑x)

= 1/12 (88,16 – (88,16)( 37,45561707)

= 9,31

m = (5 – a)/b

= (5 - 9,31)/ -0,58

= 7,431034483

(FENOL)

b = ∑xy – 1/n (∑x. ∑y)

∑x2 – 1/n (∑x)2

= 181,4089974 – 1/12 (25,45561707)( 84,85)

55,18430617 – 1/12(25,45561707)2
= 2,412427734

a = 1/n (∑y – b . ∑x)

 = 1/12 (84,85 – (2,412427734)( 25,45561707
= 2,327513616

m = (5 – a)/b

= (5 - 2,327513616)/(2,412427734)

= 1,107799561

(KLOROFORM)

b = ∑xy – 1/n (∑x. ∑y)

∑x2 – 1/n (∑x)2

= -31,53270179 – 1/12 (-4,640388672)( 76,26)

3,580549848 – 1/12 (-4,640388672)2
= -0,102

A = 1/n (∑y – b . ∑x)

= 1/12 (76,26 – (-0,102)( -4,640388672)

= 5,052

m = (5 – a)/b

= (5 - 5,052)/ -0,102

= 0,509803922

(CRUDE OIL)

b = ∑xy – 1/n (∑x. ∑y)

∑x2 – 1/n (∑x)2

=8,139660099 – 1/12 (1,455617068)( 75,43)

1,361837897 – 1/12 (1,455617068)

= -0,177226358

a = 1/n (∑y – b . ∑x)

= 1/12 (75,43 – (-0,177226358)( 1,455617068)

= 6,244005928

m = (5 – a)/b

= (5 - 6,244005928)/-0,177226358
 = 7,01930538

(OLI BEKAS)

B = ∑xy – 1/n (∑x. ∑y)

∑x2 – 1/n (∑x)2

= 314,5931417 - 1/12 (40,64510738)( 92,61)

138,0368048 – 1/12 (40,64510738)2
= 0,68

a = 1/n (∑y – b . ∑x)

= 1/12 (92,61 – (0,68)( 40,64510738)

= 3,92

m = (5 – a)/b

= (5 - 3,92)/( 0,68)

= 1,588235294

Arthemia sp

(BENZENA)
Σ XY – 1/ n (ΣX ΣY) 280,243−1/13(37,45×90,01)
𝑏= = =−0,58
ΣX2−1/n(ΣX)2 118,09−1/13(37,45)2

1(𝛴𝑌−𝑏𝛴𝑋) 1(90,01−(−0,58)×(37,45))
𝑎= = =9,31
𝑛 13

5−𝑎
𝑚= =7,43
𝑏

LC50=26979536,4

(FENOL)
Σ XY – 1/ n (ΣX ΣY) 192,37−1/13(25,45×89,34)
𝑏= = =2,41
ΣX2−1/n(ΣX)2 55,18−1/13(25,45)2

1(𝛴𝑌−𝑏𝛴𝑋) 1(89,34−𝑏×25,45)
𝑎= = =2,32
𝑛 13

5−𝑎
𝑚= =1,1
𝑏

LC50=12,81
(KLOROFORM)
Σ XY – 1/ n (ΣX ΣY) 48,47−1/13(10,14×61,67)
𝑏= = ==−0,102
ΣX2−1/n(ΣX)2 102,93−1/13(10,14)2

1(𝛴𝑌−𝑏𝛴𝑋) 1(61,67−𝑏×10,14)
𝑎= = =5,052
𝑛 13

5−𝑎
𝑚= =0,509
𝑏

Lc50=3,23

(CRUDE OIL)
Σ XY – 1/ n (ΣX ΣY) 55,53−1/13(10,14×73,13)
𝑏= = =−0,17
ΣX2−1/n(ΣX)2 44,08−1/13(10,14)2

1(𝛴𝑌−𝑏𝛴𝑋) 1(73,13−𝑏×10,14)
𝑎= = =6,24
𝑛 13

5−𝑎
𝑚= =7,01
𝑏

Lc50=10454550

(OLI BEKAS)
Σ XY – 1/ n (ΣX ΣY) 254,19−1/13(40,64×74,73)
𝑏= = =0,68
ΣX2−1/n(ΣX)2 138,03−1/13(40,64)2

1(𝛴𝑌−𝑏𝛴𝑋) 1(74,73−𝑏×40,64)
𝑎= = =3,92
𝑛 13

5−𝑎
𝑚= =1,58
𝑏

Lc50=38,74