Dexamethasone

NF-kB and MAPK inhibitor

Catalog # tlrl-dex

For research use only

Version # 13K15-MM

PrODucT iNFOrMATiON METHODS

content: Preparation of stock solution (1 mg/ml)
100 mg Dexamethasone To obtain a 1 mg/ml stock solution:
1. Weigh 5 mg of dexamethasone.
Storage and stability: 2. Add 5 ml ethanol to 5 mg dexamethasone. This provides a
- Dexamethasone is shipped at room temperature. Store solution at 1 mg/ml (2.55 mM).
at 4°C. Product is stable for 1 year when properly stored. 3. Vortex until complete solubilization.
- Solutions of dexamethasone are stable for 1 month at 4°C. 4. Aliquote and store at 4˚C.
Protect from light. 5. Once dexamethasone is solubilized, dilutions can be
prepared by adding sterile culture medium. To obtain a
solution at 20 mg/ml (51 mM), add 49 ml of culture medium
DEScriPTiON to 1 ml of the stock solution. Aqueous solutions should not be
Dexamethasone is a synthetic glucocorticoid compound with stored for more than one day.
potent anti-inflammatory activities. It represses a large set of
pro-inflammatory genes by blocking NF-kB and MAPK Working concentration: 100 nM (40 ng/ml)
activation during TLR engagement inhibits NF-kB and
specific MAPKs depending on the TLR ligand and whether the NF-kB and MAPK inhibition:
adapters MyD88 and TRIF are activated individually or To assess the role of NF-kB and MAPK, pretreat cells, such as
coincidently1. macrophages or HEK293 cells transfected with one or several
TLR genes, for 1 hour with or without dexamethasone
1. Bhattacharyya S. et al., 2010. TAK1 targeting by glucocorticoids at 100 nM and then incubate at 37°C with the appropriate TLR
determines JNK and IkappaB regulation in Toll-like receptor- ligand for 1 to 6 hours. Detect NF-kB and MAPK inhibition
stimulated macrophages. Blood. 115(10):1921-31. using the appropriate detection system.

cHEMicAl PrOPErTiES rElATED PrODucTS

cAS number: 50-02-2
Synonym: Prednisolone F Product catalog code
Formula: C22H29FO5
Molecular weight: 392.46
HO
OH
Molecular Weight: 228,24
Chemical Formula: C14H12O3
pUNO1-hTLR2 (plasmid with human TLR2 gene) puno1-htlr2
Solubility:Dexamethasone
OH

n° CAS 501-36-0
Resveratrol

Molecular Weight: 331,34

Piceatannol
Chemical Formula: C20H14FN3O
F

N
OH
N
H
N
n° CAS 152121-30-7

SB202190

pUNO-mTLR2 (plasmid with mouse TLR2 gene) puno-mtlr2

Molecular Weight: 475,62
Chemical Formula: C26H41N3O5

O O
H H
O N N
N H
H
O O

n° CAS 133407-82-6
MG 132

Molecular Weight: 244,24

Chemical Formula: C14H12O4
O OH
F H HO
H3 C H CH3 OH
HO OH
CH3
O

OH OH
n° CAS 10083-24-6
n° CAS 50-02-2

Dexamethasone Piceatannol

approximately 25 mg/ml in ethanol

Pam3CSK4 (TLR2 ligand) tlrl-pms
Appearance: White powder
n° CAS 50-02-2 pUNO-hTLR3 (plasmid with human TLR3 gene) puno-htlr3
pUNO-mTLR3 (plasmid with mouse n°
TLR3 gene)
CAS puno-mtlr3
10083-24-6
OH OH
Poly(I:C) HMW (TLR3 ligand) tlrl-pic

O
CH3
HO OH
H3 C H CH3 OH

F H HO
O OH

Chemical Formula: C14H12O4

Molecular Weight: 244,24

TECHNICAL SUPPORT
MG 132
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InvivoGen USA (International): +1 (858) 457‑5873
n° CAS 133407-82-6
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E‑mail: info@invivogen.com
O O
H
N H
O N N