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V.

HASIL DAN PERHITUNGAN

1. Perhitungan Dosis

 Mencit I Sianida (Kontrol Positif) Mencit II ( sianida + Na. Nitrit)


Larutan stok 1,5% = 1500 mg/100 ml Larutan stok 2% = 2000 mg/100 ml
Larutan sianida dosis 15 mg/kg BB tikus Larutan Na. Nitrit dosis 20 mg/kg BB tikus
BB mencit 18 g BB mencit 18 g
200 𝑔 200 𝑔
Dosis tikus = 1000 𝑔 𝑥 15 mg = 3 mg Dosis tikus = 1000 𝑔 𝑥 20 mg = 4 mg
= 3 mg x 0,14 = 4 mg x 0,14
= 0,42 mg = 0,56 mg
18 𝑔 18 𝑔
Dosis mencit = 20 𝑔 𝑥 0,42 𝑚𝑔 = 0,378 mg Dosis mencit = 20 𝑔 𝑥 0,56 𝑚𝑔 = 0,504 mg
0,378 𝑚𝑔 0,504 𝑚𝑔
Volume = 𝑥 100 𝑚𝑙 = 0,025 ml Volume = 𝑥 100 𝑚𝑙 = 0,025 ml
1500 𝑚𝑔 2000 𝑚𝑔

Mencit III ( sianida + Na. Nitrit) Mencit IV (Sianida + Na. Thiosulfat)


Larutan stok 2% = 2000 mg/100 ml Larutan stok 25% = 25000 mg/100 ml
Larutan Na. Nitrit dosis 20 mg/kg BB tikus Larutan sianida dosis 125 mg/kg BB tikus
BB mencit 18 g BB mencit 18 g
200 𝑔 200 𝑔
Dosis tikus = 1000 𝑔 𝑥 20 mg = 4 mg Dosis tikus = 1000 𝑔 𝑥 125 mg = 25 mg
= 4 mg x 0,14 = 25 mg x 0,14
= 0,56 mg = 3,5 mg
18 𝑔 18 𝑔
Dosis mencit = 20 𝑔 𝑥 0,56 𝑚𝑔 = 0,504 Dosis mencit = 20 𝑔 𝑥 3,5 𝑚𝑔 = 3,15 mg
mg 3,15 𝑚𝑔
Volume = 25000 𝑚𝑔 𝑥 100 𝑚𝑙 = 0,0126 ml
0,504 𝑚𝑔
Volume = 𝑥 100 𝑚𝑙 = 0,025 ml
2000 𝑚𝑔

Mencit V (Sianida + Na. Thiosulfat)


Larutan stok 25% = 25000 mg/100 ml
Larutan sianida dosis 125 mg/kg BB tikus
BB mencit 18 g
200 𝑔
Dosis tikus = 1000 𝑔 𝑥 125 mg = 25 mg
= 25 mg x 0,14
= 3,5 mg
18 𝑔
Dosis mencit = 20 𝑔 𝑥 3,5 𝑚𝑔 = 3,15 mg
3,15 𝑚𝑔
Volume = 25000 𝑚𝑔 𝑥 100 𝑚𝑙 = 0,0126 ml
2. Data Hasil Percobaan

Waktu (detik)
Kelompok No Sianosis Kegagalan Kejang Hilang Mati
Pernafasan Kesadaran
1. Sianida 1 315 125 105 185 262
2 65 170 150 248 315
3 98 63 35 80 68
4 - - - - -
2. Sianida + 1 201 50 34 63 121
Na. Nitrit 2 75 120 95 130 297
3 71 57 27 59 60
4 138 54 29 60 70
3. Sianida + 1 249 39 52 150 -
Na. Thiosulfat 2 97 67 39 83 225
3 149 84 50 84 90
4 500 56 32 60 86
4. Sianida + 1 190 47 45 49 110
Na. Nitrit 2 - - - - -
3 - 531 - - -
4 385 59 77 66 160
5. Sianida + 1 198 23 15 45 130
Na. Thiosulfat 2 90 54 24 57 151
3 161 70 34 - 76
4 90 44 6 35 112
Rata-Rata 153,6 85,65 42,45 72,7 116,65

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