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J. Env. Bio-Sci., 2014: Vol.

28 (2): 231-232 ISSN 0973-6913 (Print), ISSN 0976-3384 (On Line)

EFFICACY OF DIFFERENT BIO-CONTROL AGENTS AGAINST ROOT-KNOT NEMATODE


IN OKRA (ABELMOSEKUS ESCULENTUS)
Hemlata Pant
Society of Biological Sciences and Rural Development
10/96, Gola Bazar, New Jhusi, Allahabad-211019 (U.P.)
e-mail: pant_hemlata@yahoo.co.in

Received: 04-09-2014 Accepted: 15-10-2014


Various bio-control agents viz. Trichoderma viride, Paecilomyces lilacinus, Beauveria bassiana and Aspergillus niger
were taken for the experimental purpose. The result showed that higher growth parameters were observed in T. viride followed by
P. lilacinus, B. bassiana and A. niger respectively. Maximum reduction of galls were also recorded in T. viride, P. lilacinus, B.
bassiana and A. niger respectively.

Root-knot nematode (Meloidogyne incognita) is a serious plant. Data of observation was computed as per the analysis
invisible soil inhabiting pest and limitation in increasing the of variance test of completely randomized block design.
production of fruit yield in okra. Losses in yield to the extent of
RESULTS AND DISCUSSION
20-90% have been reported. Several workers have studies the
effect of different bio-control agents for management of root- In both the trails, all the treatments viz. T. viride, P. lilacinus,
knot nematode. in okra. Jatala1-5. Keeping this in view, the B. bassiana and A. niger, showed significantly higher plant
present study was undertaken on efficacy of different bio-control growth parameters and reduce number of root-knot over control.
agents against root-knot nematode in Okra. T. viride was found to be the most effective among all the
treatments. Higher plant growth parameters and minimum
MATERIALS AND METHODS number of galls were recorded in T. viride, P. lilacinus, B.
Pot as well as field trials were conducted at National Academy bassiana and A. niger respectively (Table-1 & 2). Higher number
of Biological Sciences and Rural Development (Research Wing of galls were noted under field conditions in the comparison to
of SBSRD), Allahabad farm in the year 2013-14. Fifteen pots pot conditions. Maximum number of galls were recorded in
and five micro-plots (1m2) were taken for experimental purpose. fields, this may be due to the M. incognita juveniles getting
1.5cm. diameter earthen pots were sterilized in 4% formalin optimum conditions for their multiplication and development
and filled with sterilized soil. Okra Cv. 'Kareena' were sown in (Table-2).
each pot and micro-plot. At 10 days after sowing 1000 newly The better plant growth in amended with T. viride may
hatched larva from egg masses of Meloidogyne incognita be due to ability to control many soil borne pathegen, reduce
(extracted from tomato plants) were pipetted and inoculated the root-knot infection. T. viride reducing the galls of root-knot
into three inch deep hole near the base of the plant in each pot nematode, this might be attributed to mycoparasitism and
and micro plots. 5000 spores of T. viride, P. lilacinus, B. excretion of cellular metabolites enzymes, produced antibiotics
bassiana and A. niger were also inoculated with the help of like 'viridin', that destroyed nematode population and reduced
pipet in plant zone. Plants were irrigated normally. At the vigour or aggressiveness of the inoculum4,6,8,9.
senescence stage of the crop observation was taken viz. Shoot P. lilacinus also reduce root-galls and increase plant
length, root length, fresh shoot weight, number of fruits and growth parameters due to production of 'Paecilatoxin' antibiotic
number of galls. All the bio-control agents were isolated from and egg parasitize nature of M. incognita7-9,11-12.
the local soil condition and maintained on potato dextrose
Reduction of Root-knot and increase plant growth parameters
agar slant. Fungal met along with culture filtrate was sterilized
were also observed in B.bassiana due to secretion of toxic
and 500ml. sterilized distilled water was added to make them
chemical viz. 'Beauverin' and may be also its egg parasitic
suspension. The suspension was used inoculation in okra
nature3. A. niger was also effective for minimize the number of
Efficacy of Different Bio-control Agents Against Root-knot (232)

Table-1. Efficacy of different bio-control agents against root-knot nematode in Okra (Pot condition)

Table-2. : Efficacy of different bio-control agents against root-knot nematode in Okra (Field condition)

root-galls and increase the plant growth parameters, it may management of root-knot diseases under IPM of chick-
be due to nature of toxic metabolism and release of pea. Project report submitted in DST, New Delhi (PN-SP/
'phytotoxin' chemical5,9. WS/028/2000) pp 30.
ACKNOWLEDGEMENT 6. Dennis, C. and Webster, J. (1971): Trans. Br. Mycol Soc.
The first author is grateful to Department of Science and 57: 25.
Technology, Govt. of India, New Delhi for providing financial 7. Lorito, M., Harman, G.E., Hayes, C.K., Broadway, R.M.,
assistance (Project No. WOS-B/SEED/DISHA/019/2012). Transmo, A., W oo, S.L. and Di Pietro, A. (1993):,
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