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1. General Objectives
This study tested the Plecanthus scutellarioides roots crude bark extract for its anti-pyretic
properties against the induced fever in the mice with administered with Brewer’s yeast.
2. Specific Objectives
The study will only be using Plecanthus scutellarioides crude bark extract as the treatment group in
the experiment. Th albino mice subjects, were sold from University of San Carlos, Talamban branch,
assured that each group were of the same age, sex, no deformities, healthy, and without any disease.
This is a temperture-sensitive study and its delimitation will be due to body’s own physiologic response
with external stimuli.
1. Anti-pyretic property – Any synthetic drug (processed) or herbal extracts that would have an
anti-pyretic effect or lowering of temperature back to normal. It can be tested in vivo ( Awaad et
al., 2011)
2. Acclimatization – a period of time to have the subject gain homeostasis and stability with new
environment. This will ensure proper physiologic response upon exposure to any experimental
stress (TOLEDO, 2011)
3. Body Score – observes the amount of flesh that covers the bony protuberances, which states the
variable as to its condition range from 1 (emaciation) to 5 (obesity) (Ullman and Foltz, 1999).
4. Body Weight – Though considered as an impractical tool for some study, it is still considered a
standard protocol especially for weight sensitive study (OSU, 2014)