Anti-Aging Topical Study

Using ph²™ Catechin Complex

OBJECTIVE OF THE TEST

To compare the effects of Formulas A and B on the skin moisture content, elasticity and surface
parameters of 35 female Caucasian volunteers, 14, 28 and 42 days after the daily application of
both the formulas.

SUMMARY OF PROTOCOL
The study was carried out on the inner volar aspect of the non-dominant forearm of 35 Caucasian females, in
compliance with the principles established by the World Medical Association in the Declaration of Helsinki.
The aim was to evaluate in a crossover double blind study, the effects on skin moisture content, skin surface
parameters as well as skin elasticity of a formula containing active ingredients of a natural origin in comparison with
a reference formula.
Thirty five healthy female volunteers aged from 35 to 65 years took part in the study. The washout period
started 7 days before the study commenced. During the washout and study periods, the volunteers used only
Dove soap and no other skin care products. A recording area of (2cm x 6cm =12cm²) was marked on the non-
dominant inner forearm

SKIN HYDRATION
• Both Formulas A and B hydrated 50.00

the skin at all recording time 40.00

intervals and can be regarded as
%CHANGE IN THE SKIN MOISTURE

moisturisers. 30.00

• The hydrating effect of Formula A 20.00

was superior to that of Formula B

10.00
at all recording time intervals but
this effect was statistical significant 0.00

only 28 days post application.

-10.00
• Formula A when compared to
-20.00
Formula B, showed optimum T14Days T28Days T42Days
hydrating effect at 28 days post Formula A 9.42 15.17 15.11

application and maintained the Formula B 7.83 0.90 7.84

% Change in skin moisture as a function of time after application

effect up to 42 days

anti-aging.indd 1 2014/05/09 3:36 PM

 SKIN ROUGHNESS 60.00

50.00
• Formula A reduced-, while 40.00

Formula B enhanced skin

%CHANGE IN ROUGHNESS
30.00

roughness at all recording intervals 20.00

post application. 10.00

0.00
• The difference in the effects
-10.00
of Formulas A and B on skin -20.00
roughness was statistical -30.00
significant at all recording intervals -40.00

post application. -50.00

T14Days T28Days T42Days
Formula A -5.56 -14.60 -10.56

Formula B 12.67 5.80 8.68

% Change in skin roughness as a function of time after application

SKIN SCALINESS
• Formula A reduced the skin 40.00

scaliness at all recording intervals 30.00

with the maximum effect recorded
20.00
28 days post application and
10.00
maintained the effect up to 42 days
%CHANGE IN SCALINESS

post application. 0.00

-10.00
• When compared to Formula
A, Formula B had less effect -20.00

on the reduction of scaliness. -30.00

The difference in the effects of -40.00

Formulas A and B on the reduction -10.00

of skin scaliness was statistical

-20.00
significant at all post application T14Days T28Days T42Days
Formula A -18.649 -20.360 -19.293
recording intervals.
Formula B 1.386 -8.087 -7.627

% Change in skin scaliness as a function of time after application

SKIN ELASTICITY
15.000
• Formula A showed a trend towards
enhancing skin elasticity increasing 10.000

from 14 days to 42 days post

application. 5.000
%CHANGE IN ELASTICITY

• Formula B had no significant effect 0.000

on enhancing skin elasticity and

-5.000
the trend, moving from 14 days to
42 days post application, was in a -10.000
negative direction.
-15.000
• Due to the small observed effect,
and the huge inter subject variation, -20.000
T14Days T28Days T42Days
the differences in the effects of
Formula A 0.750 0.213 1.098
Formulas, A and B was not statistical
Formula B 0.77 -1.03 0.10
significant (p values > 0.05).
% Change in skin elasticity as a function of time after application

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