Immunotec Patents

For USA Distribution only

Dear Entrepreneur,

My distinguished colleagues and I have worked, what often seems

a lifetime, to accomplish our goal; To provide you, the Immunotec
Entrepreneur, with a product (documented by these patents)
which allow us all to experience a healthier future.

It is with great pride that I extend my heartiest wishes to you for a

healthy and successful future.

Best Regards,

Dr. Gustavo Bounous

Immunotec Medical Director
 Immunotec US PATENT

U.S. Patent 5,230,902

A method of improving the active sys- enhanced resistance of target cells

temic humoral immune response in a
mammal as measured by sheep red
blood cell injection (SRBC), compris-
ing administering orally to the mammal
an effective amount of undenatured
whey protein concentrate (WPC)
obtained from bovine, goat, or sheep
milk and containing substantially all
the whey protein present in the raw
milk, administered as a daily replace-
ment for up to all the protein consumed
by the mammal, wherein the improved
active systemic humoral immune
response is based on the overall amino
acid and associated small peptides pat-
tern resulting from the contribution of
substantially all the WPC protein
components, the daily amount of WPC
not substantially exceeding the daily
protein requirement for the mammal.
The method of claim 1, wherein the
improved active systemic humoral
immune response is associated with
against the mutagenic and carcinogenic
effect of dimethlylhydrazine.
A method of producing a sustained
increase of tissue concentration level
of glutathione in a mammal, which
comprises orally administering to the
mammal an amount of undenatured
whey protein concentrate obtained
from bovine, goat, or sheep milk and
containing substantially all the whey
protein present in the raw milk as a
daily replacement for up to all the
protein consumed by the mammal,
wherein the sustained increase of the
tissue concentration level of glu-
tathione in the mammal is based on the
overall amino acid and associated
small peptides pattern resulting from
the contribution of substantially all the
WPC protein components, the daily
amount of WPC not substantially
exceeding the daily protein requirement
for the mammal.

United States Patent Patent Number: 5,230,902

Bounous et al. Date of Patent: July 27, 1993

UNDENATURED WHEY PROTEIN ABSTRACTS

CONCENTRATE TO IMPROVE
ACTIVE SYSTEMIC HUMORAL
IMMUNE RESPONSE
Inventors: Gustavo Bounous et al.
Montreal, Canada
Assignee: Immunotec Research
Corporation,
Montreal, Canada
This invention provides a method of improving the humoral
immune response or increasing the concentration levels of
glutathione in mammals, which comprises administering
orally to a mammal a therapeutically or a prophylactically
effective amount of undenatured whey protein concentrate
which has a biological activity based on the overall amino
acid and associated small peptides pattern resulting from
the contribution of all its protein components. A method for
improving the humoral immune response in mammals also
is disclosed which comprises administering orally to a
mammal the combination of a vitamin supplement contain-
ing vitamin B2 in an amount in excess of minimum daily
requirements and an effective amount of undenatured whey
protein concentrate. This invention further provides a
dietary supplement for a mammal which comprises an
effective amount of Vitamin B1, and B2 and a therapeutically
or prophylactically effective amount of whey protein
supplement.
 Immunotec US PATENT

U.S. Patent 5,290,571

A method of improving the immune response in

mammals as measured by sheep red blood cells
injection comprising administering orally to the
mammal an undenatured whey protein concentrate
obtained from bovine, goat, or sheep milk containing
substantially all the heat labile whey protein present
in the raw milk, the whey protein being present in an
amount sufficient to provide an amount of from
about 18 g to about 28 g of whey protein per 100 g of
composition, Vitamin B1 in an amount of at least 1.5
mg per 100 grams of composition, the Vitamin B1 and
Vitamin B2 being administered in amounts in excess
of minimum daily requirements.

United States Patent Patent Number: 5,290,571

Bounous et al. Date of Patent: Mar. 1, 1994

BIOLOGICALLY ACTIVE WHEY ABSTRACTS

PROTEIN CONCENTRATE
The present invention is concerned with a whey protein
composition comprising a suitable concentration of whey
Inventors: Gustavo Bounous et al. protein concentrate wherein the whey protein concentrate
Montreal, Canada contains proteins which are present in an essentially unde-
natured state and wherein the biological activity of the whey
Assignee: Immunotec Research protein concentrate is dependent on the overall amino acid
Corporation, and small peptides pattern resulting from the contribution of
Montreal, Canada all its proteins components and a method of producing said
whey protein composition. The invention also relates to
several applications of said composition.

The portion of the term of this patent subsequent to Jul. 27, 2010 has been disclaimed.
 Immunotec US PATENT
U.S. Patent 5,451,412

A method of increasing the rate of therapeutically, or a prophylactically

synthesis, rate of replenishment, and effective amount of undenatured
concentration levels of glutathione whey protein concentrate containing
in animal organs, comprising the step substantially all the heat labile whey
of administering to an animal a protein present in milk.

United States Patent Patent Number: 5,451,412

Bounous et al. Date of Patent: Sept. 19, 1995

BIOLOGICALLY ACTIVE ABSTRACTS

UNDENATURED WHEY PROTEIN
CONCENTRATE AS FOOD The present invention is concerned with a whey protein
SUPPLEMENT composition comprising a suitable concentration of whey
protein concentrate wherein the whey protein concentrate
Inventors: Gustavo Bounous et al. contains proteins which are present in an essentially unde-
Montreal, Canada natured state and wherein the biological activity of the whey
protein concentrate is dependent on the overall amino acid
Assignee: Immunotec Research and small peptides pattern resulting from the contribution
Corporation, of all its protein components and a method of producing
Montreal, Canada said whey protein composition. The invention also relates to
several applications of said composition.
The portion of the term of this patent subsequent to Jul. 27, 2010 has been disclaimed.

U.S. Patent 5,456,924

A method of treating HIV-seropositive wherein the substantially undenatured

individuals so as to increase their
blood mononuclear cell glutathione
concentration and to maintain or
increase body weight which comprises
administering to HIV-seropositive
individuals a substantially undena-
tured whey protein concentrate,
whey protein concentrate comprises
substantially all the heat labile whey
protein contained in raw milk, in an
amount effective to increase their
blood mononuclear cell glutathione
concentration and maintain or
increase body weight.

United States Patent Patent Number: 5,456,924

Bounous et al. Date of Patent: Oct. 10, 1995

METHOD OF TREATMENT OF ABSTRACTS

HIV-SEROPOSITIVE INDIVIDUALS
WITH DIETARY WHEY PROTEINS
Inventors: Gustavo Bounous et al.
Montreal, Canada
Assignee: Immunotec Research
Corporation,
Montreal, Canada
Undenatured whey protein concentrate is administered to
HIV-seropositive individuals to elevate their blood mononu-
clear cells, glutathione (GSH) level, body weight and sense
of well being. In addition T-helper cells concentration and
their T-helper cells/T-suppressor cells ratio are slightly
elevated.

The portion of the term of this patent subsequent to Jul. 27, 2010 has been disclaimed.
 Immunotec CANADIAN PATENT
Canadian Patent 1338682

A dietary supplement for a mammal cysteine thereby raising cell glu-

comprising a substantially undena-
tured whey protein isolate mixture
containing the bovine serum albumin,
alpha-lactalbumin and lactoferrin pro-
teins, which are cystine rich, said
bovine serum albumin and lactoferrin
also being rich in glutamyl cysteine,
in an amount effective to replenish
depleted cellular glutathione and
enhance the immune response.
A whey protein concentrate compris-
ing cystine to act as a precursor for
glutathione synthesis and including
heat labile protein components provid-
ing an amino acid and associated small
peptide pattern adapted significantly
to enhance the bioavailabality of the
tathione content and improving
humoral immune response.
Use of a whey protein composition for
improving the immune response in
mammals, wherein the whey protein
concentrate comprises a substantially
undenatured whey protein isolate
mixture which is cystine rich and rich
in glutamyl cysteine, in an amount
effective to replenish depleted cellular
glutathione and enhance the immune
response.

Canadian Patent No.: 1338682

BIOLOGICALLY ACTIVE UNDENATURED

WHEY PROTEIN CONCENTRATE AS FOOD
SUPPLEMENT

Inventors: Gustavo Bounous et al.

Montreal, Canada

Assignee: Immunotec Research

Corporation,
Montreal, Canada
Printed in Canada