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Food Safety Online Ordering

Foodborne Bacteria www.neogen.com/foodsafetyorder.htm

NEO-GRID™ / ISO-GRID™
For Enumeration of Total E. coli and E. coli O157:H7

Durable Results
ISO-GRID
Disposable
System
NEO-GRID
System

SD-39 Agar
Intended Use Proteose Peptone SM47 5 g/L
Using SD-39 Agar, the ISO-GRID and NEO-GRID Membrane Filter Yeast Extract 3 g/L
Systems are used to provide a method for enumeration of E. coli and Sodium Chloride 5 g/L
E. coli O157:H7. L-Lysine Monohydrochloride 10 g/L
Dextrose 2.5 g/L
The Test
Sorbitol 20 g/L
The ISO-GRID and NEO-GRID systems are based on the principle Magnesium Sulfate 7-Hydrate 1.5 g/L
of hydrophobic grid membrane filtration. A sample’s total E. coli and Monensin 0.038 g/L
E. coli O157:H7 is enumerated through the use of a unique membrane Sodium Glucuronate 0.5 g/L
filter containing a grid of 1,600 squares. First, a diluted sample is
Novobiocin 0.0075 g/L
prefiltered to eliminate any food particles present. The sample is then
Phenol Red 0.12 g/L
filtered through a hydrophobic membrane, and the membrane is placed
on SD-39 Agar. SD-39 Agar is specifically formulated to enumerate X-glucose 0.05 g/L
a sample’s total E. coli and E. coli O157:H7. Agar 15 g/L
Final pH: 7.2 ± 0.2
After incubation the membrane filter is examined, and all squares
containing one or more pink and/or green colonies are counted. The
total number of positive squares is converted to the corresponding most
probable number (MPN) using one of the methods described in the Ordering Information
ISO-GRID Methods Manual, and the total E. coli MPN per gram is
Prod.# Product description
calculated. A presumptive E. coli O157:H7 MPN per gram is calcu-
For Use With Either the NEO-GRID or ISO-GRID Test System
lated by counting all the squares containing one or more pink colonies
6907 SD-39 Agar
and following the same procedure. The presence of E. coli O157:H7
6810 1-Place Filtration Manifold
can be confirmed using the confirmation procedures described in the
6800 3-Place Filtration Manifold
Methods Manual.
6801 6-Place Filtration Manifold
6821 Rubber Tubing (10 feet)
Test Procedure
6822 Side-Arm Flask Assembly (1 L)
1. Prepare a sample homogenate in a specified diluent. 6824 Vacuum Pump - ¼ hp (Gast)
2. Filter 1 mL of the homogenate through the ISO-GRID or 6828 Forceps
NEO-GRID membrane filter. For Use With The NEO-GRID Test System Only
3. Place the membrane filter on the surface of SD-39 Agar. 6848 NEO-GRID Test System - Contains 48 individually
4. Incubate inverted plate for 24 hours at 44.5°C. packaged membrane filtration systems
Refer to the Methods Manual for complete instructions, and other avail- 6825 NEO-GRID Silicone Stopper
able test methods. 6826 Filta Tips (box of 100)
6827 Filter Bags - 24 oz Whirl-Pak (box of 250)
Product Specifications 6829 1.0 mL Sterile Serological Pipettes (bag of 50)
Approvals: AOAC Official Method #997.11 For Use With The ISO-GRID Test System Only
Please see page 95 for the required ISO-GRID equipment and
accessories.

38 Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com

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