KOVA GLASSTIC SLIDE 10 WITH GRIDS

INSTRUCTIONS FOR USE

The KOVA Glasstic Slide 10 with quantitative grid is designed to be used with the standardized hygienic KOVA Microscopic Urinalysis System:

Fill the KOVA Tube to 12mL and firmly attach the KOVA Cap. Centrifuge at 400 rcf (-1500 rpm) for 5 minutes.

Insert the KOVA Petter firmly and decant. 1.0mL of sediment will be trapped by the KOVA 1 Petter.

Gently resuspend using the KOVA Petter. If desired, add 1 drop of KOVA Stain prior to resuspension.

Using the KOVA Petter, transfer the sample to the notch on the slide chamber. Carefull addition of samples insures the hygienic handling properties of the KOVA System.

By capillary action 6.6 L of the sample will be drawn into the KOVA Slide2 10 chamber resulting in a homogenous suspension of the sediment.

Quantitate the casts at low power (100x). Quantitate all cells at high power (400x). Count the cells within the lines of the small 0.33 mm square grid (as shown). Refer to the value table for the cell count per L of patient sample.

VALUE TABLE
Low Cell Count Samples: Count the total cells of a specific type contained in 10 small grids within different quadrants of the counting grid. Total Cells 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Cells / L 1 2 2 3 4 5 5 6 7 8 8 9 10 11 11 12 13 14 15 15 16 17 18 18 19 20 21 21 Higher Cell Count Samples: Count the total cells of a specific type contained in 5 small grids within different quadrants of the counting grid. Total Cells 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 30 35 40 45 50 60 70 Cells / L 8 9 11 12 14 15 17 18 20 21 23 24 26 28 29 31 32 34 35 37 38 46 54 61 69 77 92 107

KOVA Glasstic Slide 10 with Grid Chamber CAT/REF: 87144 Chamber Volume: 6.6L Chamber Depth: 0.1 mm Outer Grid Dimension: 3 mm x 3 mm Volume within Grid: 0.9L Small Grid Size: 0.33 mm x 0.33 mm Small Grid Volume: 0.01111L

NOTE: For samples that are less than 12mL, reduce the centrifuged quantity to 6mL and double the results obtained before using the table (above). Cell Type Leukocytes Erythrocytes Normal 0-4/L 0-2/L Borderline 4-6/L 2-3/L Pathological* > 6/L > 3/L

Alternative Calculation: Determine the average number of cells per small grid and then use the following multiplication factor to calculate the cells per L.

To calculate cells / L using KOVA Glasstic Slide 10 with Grid:

For uncentrifuged or neat samples, multiply the average cells obtained per grid x 90. For 10mL samples concentrated to 1mL, multiply the average cells obtained per grid x 9. For 10mL samples concentrated to 0.5mL, multiply the average cells obtained per grid x 4.5. For 12mL samples concentrated to 1mL (KOVA System), multiply the average cells obtained per grid x 7.5.

Calculation example (Using KOVA System 12mL to 1mL method): Cells Leukoytes Erythrocytes Grids Counted 10 10 Total Cells 5 14 Average Cells / Grids 0.5 1.4 Multiple x Factor (7.5) 0.5 x 7.5 1.4 x 7.5 Cells per L of Samples 3.8 10.5

* Reference: Aiken, C.D. and Sokeland, J. (1983). Urologie. Thiems, Stuttgart, Ninth Edition, p.79

KOVA GLASSTIC SLIDE 10 WITH GRIDS

VALUE TABLE UNDILUTED, UNCENTRIFUGED URINE OR BODY FLUID SPECIMENS
LOW CELL COUNT SAMPLES Count the total cells of a specific type contained in 36 small grids or 4 complete quadrants of the counting grid. Total Cells 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 25 30 40 50 Cells/L 3 5 8 10 13 15 18 20 23 25 28 30 33 35 38 40 43 45 48 50 63 75 100 126 Cells/mL 2,500 5,000 7,500 10,000 12,500 15,000 17,500 20,000 22,500 25,000 27,500 30,000 32,500 35,000 37,500 40,000 42,500 45,000 47,500 50,000 62,500 75,000 100,000 125,500 HIGH CELL COUNT SAMPLES Count the total cells of a specific type contained in 10 small grids in different quadrants of the counting grid. Total Cells 1 2 3 4 5 6 7 8 9 10 20 25 30 35 40 50 60 70 80 90 100 150 200 250 Cells/L 9 18 27 36 45 54 63 72 81 90 180 225 270 315 360 450 540 630 720 810 900 1350 1800 2250 Cells/mL 9,000 18,000 27,000 36,000 45,000 54,000 63,000 72,000 81,000 90,000 180,000 225,000 270,000 315,000 360,000 450,000 540,000 630,000 720,000 810,000 900,000 1,350,000 1,800,000 1,250,000

Alternative Calculation: Multiply the average number of cells per small grid by 90 to obtain cells per L; multiply by 90,000 to obtain cells per mL.

Alternative Calculation: Multiply the average number of cells per small grid by 90 to obtain cells per L; multiply by 90,000 to obtain cells per mL.

DILUTED BODY FLUIDS CALCULATION METHOD: Cells / L = Average number of cells per small grid x 90 (multiplication factor) x dilution e.g., Spinal fluid diluted 1:10; a total of 50 RBCs counted in 10 small grids RBC/L = 50 cells 10 grids x 90 (factor) x 10 (dilution)

= 5 x 900 = 4,500 RBCs/L e.g., Semen diluted 1:20; a total of 150 sperm counted in 5 small grids Sperm/L = 150 5 x 90 (factor) x 20 (dilution)

= 30 x 1800 = 54,000 sperm/L TOTAL CELL COUNT NORMAL RANGES (1) FLUID Urine (2) CSF (Adult Range) Seminal CELL TYPE Leukocytes Erythrocytes Leukocytes Sperm NORMAL 0-6/L 0-3/L 0-5/L 40,000/L - 160,000/L ABNORMAL > 6/L > 3/L > 5/L < 40,000/L FLUID Synovial Pleural Pericardial Pertoneal CELL TYPE Leukocytes Erythrocytes Leukocytes Leukocytes Leukocytes Erythrocytes NORMAL < 200/L < 2,000/L < 1,000/L < 1,000/L < 300/L < 100,000/L ABNORMAL > 200/L > 2,000/L > 1,000/L > 1,000/L > 300/L > 100,000/L

References: (1) Strasinger, S.K. (1985) Urinalysis and Body Fluids, F.A. Davis, Philadelphia (2) Alken, C.D., and Sokeland, J. (1983) Urologie, Thiems, Stutgart, Nineth Edition, pg. 79

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1. U.S. Patent Number: 4,563,332 2. U.S. Patent Numbers: 4,997,268 5,128,802 KOVA and GLASSTIC are registered trademarks of Hycor Biomedical Inc.

PN 91064-09 Rev. 7/05