Professional Documents
Culture Documents
Bioprocess Systems
Biochemical Engineering
Introduction
In the biochemical engineering profession, there are various
bioprocesses actively involved in the synthesis and production of
biological products.
Understanding of all the processes may require basic knowledge
of biology, biochemistry, biotechnology, and real knowledge
of engineering processes.
Transfer of oxygen is a major concern in many bioprocesses that
require air for microbial growth such as single cell protein and
production of antibiotics.
Agitation in a fermentation unit is directly related to
oxygen transported from the gas phase to liquid phase followed
by oxygen uptake by the individual microbial cell.
The activities of microorganisms are monitored by the utilization
of oxygen from the supplied air and the respiration quotient.
The primary and secondary metabolites in a bioprocess can be
estimated based on projected pathways for production of
intracellular and extracellular by-products.
Solubility
The solubility of air in water at 10 C and under atmospheric
conditions is 11.5 ppm; as the temperature is increased to 30 C,
the solubility of air drops to 8 ppm. The solubility of air decreases
to 7 ppm at 40 C.
Availability of oxygen in the fermentation broth is higher than the
air, if pure oxygen is used. The solubility of pure oxygen in water
at 10 C and 1 atm pressure is 55 ppm.
As the temperature increases to 30 C, the solubility of pure
oxygen drops to 38.5 ppm. The solubility of pure oxygen
decreased to 33.7 ppm at 40 C.
The above data show that in case of high oxygen demand for SCP
(microbial protein) production, oxygen drastically depletes in 12
24 hours of incubation.
Therefore pure oxygen is commonly used to enhance oxygen
availability in the fermentation media.
Measurement of DO
The concentration of dissolved oxygen in a fermenter is
normally measured with a dissolved oxygen electrode,
known as a DO probe.
There are two types in common use:
galvanic electrodes and polarographic electrodes.
where NA is the oxygen flux in kmolm2 s1, kL is the liquid side mass
transfer coefficient
in ms1, CAL * is the oxygen concentration in equilibrium with the
liquid phase at the interface in kmol/m3, CAL is the oxygen
concentration in the bulk of liquid in kmol/m3, and a is the interfacial
area in surface area of bubbles per unit volume of broth (m2/ m3).
RESPIRATION QUOTIENT
(RQ)
Measurements of inlet and outlet gas compositions of a culture vessel
have been considered as an indicator for cell activities in the
fermentation broth.
The continuous monitoring of gas analysis would lead us to understand
the oxygen consumption rate and carbon dioxide production, which
originate from catabolism of carbon sources.
Respiration is a sequence of biochemical reactions resulting in electrons
from substances that are then transferred to an exogenous electron
accepting terminal.
Respiration in a cell is an energy-delivery process in which electrons are
generated from oxidation of substrate and transferred through a series of
oxidationreduction reactions to electron acceptor terminals.
In biosynthesis, the end products result from a respiration process. Since
oxidation of carbonaceous substrate ends with carbon dioxide and water
molecules, the molar ratio of carbon dioxide generated from oxidation
reduction to oxygen supplied is known as the respiration quotient:
Problem
EFFECT OF AGITATION ON DO
Aerobic bacteria are easily grown at a small scale in tubes and flasks by
incubating the media under normal atmospheric conditions.
In large-scale operations, the media has to be exposed to air, and
sufficient air must be present for respiration of all living microorganisms.
The indication of availability of oxygen in the liquid phase is to measure
the amount of dissolved oxygen.
DO probes are available on the market, and most fermenters are
equipped with a DO meter.
For aerobic fermentation, the bioreactor must be equipped with a DO
meter.
The level of DO in the media is a function of temperature. Higher
operating temperatures decrease the level of DO.
To have sufficient oxygen, an air sparger is required to purge
compressed air or pressured air to be bubbled into the media.
The availability of oxygen is a major parameter to be considered for
effective microbial cell growth rate.
AIR SPARGER
Air under pressure is supplied through a tube end consists of an O ring with very
fine holes or orifices.
The size of bubbles depends on the size of hole and type of sparger.
For very fine bubbles with effective gas dispersion, a micro-sparger is used in the
fermenter.
A micro-sparger is in fact a highly porous ceramic material and is used instead of
a gas sparger.
The size of bubbles affects the mass transfer process. Smaller bubble size
provides more surface area for gas exposure, so a better oxygen transfer rate is
obtained.
The size of gas bubbles and their dispersion throughout the tank are critical to
bioreactor performance.
Although a sparging ring will initially provide smaller size and better gas
distribution with sufficient agitation, micro-spargers are often used because the
porous media provides an extensive number of fine and uniform bubbles.
They are also resistant to plugging of biomass on the outer surface of the sparger.
Gas dispersion is not mainly related to the sparger, but rather it is dependent on
the type of impeller used for agitation.
Agitation creates uniformity of gas bubbles in the entire media by placing the
agitator in the appropriate position.
where
NA is the oxygen flux in kmolm2 s1, kL is the liquid side mass transfer coefficient in m/s,
CAL * is the oxygen concentration in equilibrium with the liquid phase at the interface
in kmol/m3,
CAL is the oxygen concentration in the bulk of liquid in kmol m3,
a is the interfacial area in surface area of bubbles per unit volume of broth (m2 m3).
OTR
In the large
bioreactor, the
partial pressure of
oxygen in the gas
will fall as it passes
through the
fermentation broth.
The equilibrium
concentration is
evaluated from
Henrys law.
The equilibrium
concentration of
oxygen is calculated
by the ratio of mean
value of pressure
over Henrys law
constant, H.
ANTIFOAM
Gas Hold Up