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Weil, Bolton, and Wertz, 1994, Electron Paramagnetic Resonance, New York: Wiley Interscience.
No hyperfine
10 Gauss
H
O.
HC CH2
NHR
H
Gunther, M.R., Sturgeon, B.E., and Mason, R.P., Free Radic. Biol. Med. 28:709-719, 2000
H3C
H3C
N
O
H3C
H3C
H
N
O.
PBN/4-POBN, phenyl-N-t-butylnitrone
C N C(CH3)3
H O
R.
R
C N C(CH3)3
H O.
C N C(CH3)3
H O.
13CR
O N
2 0 G a u s s
C N C(CH3)3
H O.
R.
.O N C(CH3)3
R
3,5-dibromo-4-nitrosobenzene sulfonate
Br
Br
SO3-
O N
R. .
O N
SO3-
R
Br
Br
40 Gauss
CH3
CH3
H
N
O.
CH2R
17O-labeled tyrosine
O.
R
O.
R
CH3
C
CD3
N
C
CH3
CH3
CD3
CD3
H
H NH2
MNP/.tyrosine
.O
C CH
H
H H
MNPd9/ tyrosine
.O
OH
(CH3)3C
N
O.
CH2R
H CO2-
H NH2
C CH
H CO2-
H
N
O
ROH
H3C
Men+
H3C
H
N
O
H
OR
- H+ H3C
- eH3C
H
N
O.
OR
Summary
The main feature of EPR spectra that is useful for
assignment to a particular free radical structure is
hyperfine splitting
Direct EPR spectra can provide a wealth of structural
information
Highly unstable free radicals can, in many cases, be
stabilized for EPR characterization by spin trapping
The increased stability of the detected free radical comes with a
loss of structural information
The adduct may undergo chemistry between formation and
detection
Adduct assignment is assisted by selective isotope labeling and
EPR analysis of an independent preparation of the suspected
adduct
The performance of appropriate controls is essential