Vit Ting Anh Hc Thut

T chc lp vit bo khoa hc ng trn tp ch

quc t (2)
(Biotechnology)
Kha Thi c
i hc Y Dc TP H Ch Minh Gim c trung tm vit bo khoa hc bng
ting Anh

http://www.chineseowl.idv.tw

Tiu s c nhn
Kha Thi c (Ted Knoy) dy vit ting Anh k
thut trong cc trng i hc i Loan hn hai
mi nm. ng l tc gi ca mi bn cun sch
v vit ting Anh k thut v chuyn nghip. ng
thnh lp mt trung tm vit ting Anh ti trng i
hc Y Yunpei ng thi cng l ging vin ton thi
gian ti trng. ng chnh sa trn 55,000 bi
vit cho vic ng bo nghin cu khoa hc t nm
1989. ng l cng nh bin tp ting anh cho mt s
tp ch v khoa hc, k thut v y hc ca i Loan.

A. Nn tng (Background)

Thit lp cc xut nghin cu (Setting of research proposal): M t

mt xu hng ph bin, pht trin hoc hin tng trong lnh vc ca bn
ngi c c th hiu c bi cnh m bn xut nghin cu ang
c thc hin .

Vn nghin cu (Research problem) : M t cc hn ch chnh hoc

tht bi ca cc nghin cu trc y hoc cc phng php nghin
cu khi gii quyt cc xu hng, pht trin hoc hin tng nu .

c im k thut nh lng ca vn nghin cu (Quantitative

specification of research problem): nh lng hoc a ra mt v d v
vn nghin cu c trch dn trong ti liu tham kho trc .

Tm quan trng ca vn nghin cu (Importance of research

problem) : M t cc hu qu v mt l thuyt v thc t nu khng gii
quyt vn nghin cu.

B. Thc hin (Action)

Mc tiu nghin cu (Research objective) : M t mc tiu ca nghin

cu xut ca bn v bao gm cc c im chnh ring bit ca
nghin cu t c mc tiu nghin cu , iu m khng c
thc hin trong nghin cu trc y ( mt cu )

Phng php t c mc tiu nghin cu (Methodology to

achieve research objective) : M t ba hoc bn bc chnh t
c mc tiu nghin cu ca bn .

Kt qu d kin ( Anticipated results) : M t cc kt qu nh lng

m bn hy vng s t c trong nghin cu ca bn.

ng gp trong lnh vc l thuyt v thc tin (Theoretical and

practical contribution to field) : M t cch thc phng php hoc
kt qu nghin cu xut ca bn s ng gp v mt l thuyt trong
lnh vc nghin cu, quy lut v cng ng gp thit thc trong sn
xut, ngnh cng nghip dch v.

V d 1: Biotechnology
Thit lp cc xut nghin cu As a steroid hormone, cortisol is the main
peripheral secretory product of the hypothalmic pituitary adrenal stress
neuroendocrine axis. The cortisoldiurnal pattern fluctuates constantly, necessitating
the search for a fast reacting and commercially available cortisol.
Vn nghin cu While cortisol is normally administered as a serum,
conventional sampling methods are invasive. Additionally, using the ELISA kit for
examination is expensive.
c im k thut nh lng ca vn nghin cu For instance, using the
commercially available ELISA kit to examine the serum is markedly more expensive
than using a customized procedure to examine the saliva. Moreover, examining the
serum requires more than two days.
Tm quan trng ca vn nghin cu The inability to examine the cortisol with a
customized ELISA kit will make the cost prohibitively high; in addition, the sampling
procedure must be an invasive one. Moreover, the inability to reduce the sampling
time may lead to hematolysis and interference with the serum during examination.

V d 1 (cont.)
Mc tiu nghin cu Based on the above, we should develop an
accurate and non-intrusive diagnosis method to examine human
diseases efficiently.
Phng php t c mc tiu nghin cu To do so, saliva can
be sampled using a non-intrusive diagnosis method. An accurate and
feasible diagnosis produced can then be adopted that is compatible
with the customized ELISA kit. Next, the steroid examination value can
be derived to diagnose the disease accurately and efficiently.
Kt qu d kin As anticipated, the customized and non-intrusive
ELISA kit can diagnose diseases rapidly and accurately, subsequently
reducing the likelihood of a hemolytic sample by roughly 30%.
ng gp trong lnh vc l thuyt v thc tin Importantly, the
steroid examination value derived by the proposed diagnosis method
can provide a valuable reference for clinically diagnosing human
diseases.

V d 2: Biotechnology
Thit lp cc xut nghin cu A disposable microbial based sensor
attempts to control the quality of urea in milk. The biocomponent part of the
area biosensor is an immobilized urease yielding a bacterial cell biomass
isolated from soil, and is coupled to the ammonium ion selectie electrode of a
potentiometric transducer. The membrane potential of all potentiometric cell
based probes is related not only to the activity of not only electrochemically
detected product, but also to the substrate of the Nernst equation.
Vn nghin cu Given the growing public concern over urea in milk
(commonly referred to as synthetic milk), urea functions as an adulterant since
it is not a natural constituent of milk. Urea in milk of dairy cattle leads to
excessive N uptake and rumen efflux of crude protein intake, thus reducing the
fertility rates among dairy cattle.
c im k thut nh lng ca vn nghin cu The inability of the
food sector to provide rapid, reliable and inexpensive analytical methods for
controlling the quality of urea in milk makes wet chemistries and analytical
practices time consuming, labor intensive and expensive.
Tm quan trng ca vn nghin cu Above limitations have led to
heightened consumer concern over food composition and safety.

V d 2 (cont.)
Mc tiu nghin cu Based on the above, we should develop a microbial
based biosensor to determine the presence of urea in milk.
Phng php t c mc tiu nghin cu To do so, urea biosensor
can be identified as an immobilized urease yielding bacterial cell biomass
isolated from soil, and can be coupled to the ammonium ion selective
electrode of a potentiometric transducer. The membrane potential of all types
of potentiometric cell based probes can be correlated with the activity of
electrochemically detected product, and thus to the activity of the substrate by
a form of the Nernst equation..
Kt qu d kin As anticipated, the proposed microbial based biosensor can
analyze milk samples for the presence of urea with a response time as long as
2 min. Those results can correlate well with those of a pure enzyme system.
ng gp trong lnh vc l thuyt v thc tin Importantly, the microbial
based biosensor has the advantages of a high specificity, portability, simplicity,
reliability and continuous real time analysis. Furthermore, an adequate
correlation of those results can be made between a pure enzyme system and
the spectrophotometric methods.

V d 3: Biotechnology
Thit lp cc xut nghin cu A wound dressing attempts to ensure optimal healing
by easing pain and preventing trauma infection. Composition of the wound dressing and
its adherence to the skin largely deteriorate its therapeutic effectiveness. Additionally,
composition of the medicine enables the wound to heal, while the medicine carrier
ensures the staying power of the wound dressing on the skin.
Vn nghin cu Wounded skin often destroys its collagen, necessitating that the
wound dressing contains collagen. Also, collagen extracted from an animal organism
often requires adding other materials to facilitate extraction, possibly causing the user to
become allergic to the wound dressing.
c im k thut nh lng ca vn nghin cu As is widely assumed, cysteine
protease is the predominant class of protease in plants. Its use in medicine can help to
remove wound bacterium and shorten treatment time, providing alternatives that
conventional methods are unable to.
Tm quan trng ca vn nghin cu The inability to resolve the potential threat of
anaphylaxis may incur other adverse side effects and reduce the effectiveness of
therapeutic treatment.

V d 3 (cont.)
Mc tiu nghin cu Based on the above, we should develop a novel
composition from plants that is more effective than animal collagen in
reducing the likelihood of anaphylaxis. Additionally, the proper carrier can
be used to transport the composition in order to maintain its therapeutic
effectiveness.
Phng php t c mc tiu nghin cu To do so, cysteine
protease can be extracted and purified from sesame. Polysaccharide can
then be purified from the fruiting body of an edible Jews Ear mushroom.
Next, the complex can be applied to the wound through the interaction
between cysteine protease and polysccharide.
Kt qu d kin As anticipated, the stable formation of the novel
composition can function as an entrapping reservoir for active ingredients
that can be sustained to ensure a prolonged release.
ng gp trong lnh vc l thuyt v thc tin In addition to its
economic benefits, the novel composition proposed herein can not only
treat wounds but, more importantly, reduce the incidence rate of
anaphylaxis.

V d 4: Biotechnology
Thit lp cc xut nghin cu Recent changes in the daily diets of
Taiwanese towards foods with high cholesterol contents and high energy,
along with sedentary life styles, have increased the annual rate of thrombosis.
Anti-platelet aggregation and development of an anti-coagulation drug are of
priority concern in combating thrombosis.
Vn nghin cu However, given the many categories of anticoagulation
drugs, the adverse effects of commonly administered drugs is too high, while
the sensitivity of traditional platelet function assays is too low.
c im k thut nh lng ca vn nghin cu For instance, using
radioimmunossay determination, only PF4 basal levels of 10-50 ng/ml are
obtained. However, a highly sensitive determination of PF4 by ELISA could
detect as little as 0.6 ng/ml of PF4 basal levels.
Tm quan trng ca vn nghin cu The inability to alleviate these
adverse effects will lead to hemorrhaging or allergies following administration
of anticoagulation drugs. Moreover, the inability to increase the sensitivity of
determination makes it impossible to detect the lowest levels of platelet
formation.

V d 4 (cont.)
Mc tiu nghin cu Based on the above, we should develop a highly
sensitive determination method, capable of assessing the lowest levels of
secretion from an activated platelet and facilitating efforts to develop a new
anticoagulation drug.
Phng php t c mc tiu nghin cu To do so, anti-platelet
aggregation compounds can be isolated from Chinese herbal medicine.
Secretion from an activated platelet can then be assessed, demonstrating
several biological activities in these compounds, including antioxidant activity
and anti-inflammation. Next, the role of inhibited stimulators in inducing platelet
aggregation to developing an anticoagulation drug can be assessed.
Kt qu d kin As anticipated, the proposed determination method can
facilitate the use of Chinese herbal medicine as an anticoagulation drug by
reducing adverse effects by at least 80%.
ng gp trong lnh vc l thuyt v thc tin In addition to contributing to
the effectiveness of Chinese herbal medicine in preventing thrombosis-related
complications and myocardial infarction, the proposed determination method
can assess the lowest levels of secretion from an activated platelet and detect
inherited deficiencies.

Ti liu tham kho

Knoy, T (2002) Writing Effective Work
Proposals. Taipei: Yang Chih Publishing

Further details can be found at

http://www.chineseowl.idv.tw