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HEMATOCYTOMETER or NEUBABUER
CHAMBER
NEUBAUERS SLIDE
- Thick slide with two ruling
area on center
- Ruled area are separated by
H- shaped
gutter/trough/depression
- Beyond the two vertical arm
of trough there are two raised
shoulder (ridge) which
support cover glass
- Lining is coated by shining
metal or rhodium
HEMATOCYTOMETER or NEUBABUER
CHAMBER
Consists of TWO CHAMBERS (COUNTING CHAMBER)
One chamber is divided into 9 large squares with
dimensions of 1x1mm
A cover glass (0.1mm) is supported over these squares
TOTAL VOLUME OF EACH SQUARES
1.00 mm x 0.1mm or
0.1mm3 or
10-4 cm3
COUNTING RULES
DO NOT COUNT CELLS
TOUCHING:
Bottom line
Right line
This is to avoid double counting
Guide Questions:
2.
5.
REFERENCES
http://www.slideshare.net/GovardhanJoshi/hemocytomet
er?qid=5ddd9dcc-48cb-4d15-b930c3a4188658e5&v=&b=&from_search=2
https://en.wikipedia.org/wiki/Hemocytometer#/media/Fil
e:Haemocytometer_Grid.png
Laboratory Manual in Cell Molecular Biology