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Examine color of
slant and tube:
reaction that
took place
Incubate at
35C for 24
hours
Examine
tubes:
bubbles and
cracks = CO2/
H2
Examine
tubes:
blackening =
production of
H2S
Interpretation of Results
Reaction
Production of H2S
Production of CO2/ H2
bubbles or cracks
no bubbles or cracks
Interpretation
K/NC
K/K
K/A
A/A
Experimental
E. coli
no black spots in
medium
(-) H2S
(-) H2S
no
breakage/bubbles
A/A
fermentation of
lactose/sucrose and
glucose
A/A: glucose,
lactose /sucrose
fermenter gas
no black spots in
medium
(-) H2S
(-) H2S
presence of bubbles
A/A
fermentation of
lactose/sucrose and
glucose
K/NC: glucose,
lactose and
sucrose nonfermenter
P.
auruginosa
Theoretical
D. Production of acetyl-methyl-carbinol
(Voges-Proskauer Test) and mixed acids
(Methyl Red Test) from Glucose
(MR-VP Test)
Principle
MR Test
Principle
VP test
Procedure
Inoculate
organisms into
MR-VP broth
(replicates)
Incubate at
35C for 48
hours
Set 2: VP test
+12drps of 5% alpha
naphthol in absolute ethyl
alcohol
+4drps of 40% KOH
Set 1: MR test
+5drps
methyl red
Interpretation of Results
Test
(+)
Positive
Result
(-) Negative
Result
Positive and
negative results
for MR test
MR: mixedacid
remains
turns red
fermentatio
yellow
n
VP:
remains
presence of turns red
yellow
diacetyl
Positive and
negative results
for VP test
E. coli
P.
aeruginos
a
MR test
VP test
EXP
EXP
THE
O
THEO
+ + -