Professional Documents
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Creatinine and
residual nitrogen
Dr Tehani Silva
Creatine
Enzyme
In
Creatine
Creatinine vs Creatine
Importance of creatinine
Importance of creatinine
Muscle mass
Amount of protein in the diet
1.
2.
Objectives
Creatinine clearance test
Determination of residual nitrogen in the
blood
Jaffe reaction
Procedure
Creatinine + Picric acid (yellow colour)
Procedure
Standard Test
(ml)
(ml)
Blank
(ml)
Serum
0.5ml
Water
1.5ml
1.5ml
2ml
Standard
for serum
0.5ml
Picric acid
6ml
6ml
6ml
Calculation
Serum creatine = Absorbance of test X conc. of standard
Absorbance of standard
Increase amount
Renal failure
Advance liver failure
Tissue damage (e.g: Gangrene, malignant
tumors, compartment syndrome)
Heamodylisis
2.
Determination of residual
nitrogen of blood
The interaction of ammonia sulfate with the
Nessler Reagent, which contains the
mercury salt, leads to the appearance of
the yellow-orange product.
The intensity of its color directly
proportional to the concentration of
residual nitrogen.