DNA must be replicated before a cell divides to ensure each new cell receives a full copy of genetic information. DNA replication involves unwinding the double helix at the replication fork, adding RNA primers, and synthesizing new strands with DNA polymerase in the 5' to 3' direction. Once replication is complete, primers are removed and DNA ligase seals the gaps. Transposable elements can cause mutations by inserting into genes and altering DNA sequences through deletion or inversion during transposition and recombination.
DNA must be replicated before a cell divides to ensure each new cell receives a full copy of genetic information. DNA replication involves unwinding the double helix at the replication fork, adding RNA primers, and synthesizing new strands with DNA polymerase in the 5' to 3' direction. Once replication is complete, primers are removed and DNA ligase seals the gaps. Transposable elements can cause mutations by inserting into genes and altering DNA sequences through deletion or inversion during transposition and recombination.
DNA must be replicated before a cell divides to ensure each new cell receives a full copy of genetic information. DNA replication involves unwinding the double helix at the replication fork, adding RNA primers, and synthesizing new strands with DNA polymerase in the 5' to 3' direction. Once replication is complete, primers are removed and DNA ligase seals the gaps. Transposable elements can cause mutations by inserting into genes and altering DNA sequences through deletion or inversion during transposition and recombination.
DNA is the genetic material that defines every cell.
Before a cell duplicates and is divided into new daughter cells through either mitosis or meiosis, biomolecules and organelles must be copied to be distributed among the cells. DNA, found within the nucleus, must be replicated in order to ensure that each new cell receives the correct number of chromosomes. The process of DNA duplication is called DNA Replication. DNA Structure
DNA or deoxyribonucleic acid is a type of molecule
known as a nucleic acid. It consists of a 5-carbon deoxyribose sugar, a phosphate, and a nitrogenous base. Double-stranded DNA consists of two spiral nucleic acid chains that are twisted into a double helix shape. This twisting allows DNA to be more compact. In order to fit within the nucleus, DNA is packed into tightly coiled structures called chromatin. Chromatin condenses to form chromosomes during cell division. Prior to DNA replication, the chromatin loosens giving cell replication machinery access to the DNA strands. Preparation for Replication
1. Replication Fork Formation
2. Primer Binding 3. Elongation 4. Termination 1.Replication Fork Formation Before DNA can be replicated, the double stranded molecule must be “unzipped” into two single strands. DNA has four bases called adenine (A), thymine (T), cytosine (C) and guanine (G) that form pairs between the two strands. Adenine only pairs with thymine and cytosine only binds with guanine. In order to unwind DNA, these interactions between base pairs must be broken. This is performed by an enzyme known as DNA helicase. DNA helicase disrupts the hydrogen bonding between base pairs to separate the strands into a Y shape known as the replication fork. This area will be the template for replication to begin. 2.Primer Binding The leading strand is the simplest to replicate. Once the DNA strands have been separated, a short piece of RNA called a primer binds to the 3' end of the strand. The primer always binds as the starting point for replication. Primers are generated by the enzyme DNA primase. 3. Elongation Enzymes known as DNA polymerases are responsible creating the new strand by a process called elongation. There are five different known types of DNA polymerases in bacteria and human cells. In bacteria such as E. coli, polymerase III is the main replication enzyme, while polymerase I, II, IV and V are responsible for error checking and repair. DNA polymerase III binds to the strand at the site of the primer and begins adding new base pairs complementary to the strand during replication. In eukaryotic cells, polymerases alpha, delta, and epsilon are the primary polymerases involved in DNA replication. Because replication proceeds in the 5' to 3' direction on the leading strand, the newly formed strand is continuous. 4.Termination Once both the continuous and discontinuous strands are formed, an enzyme called exonuclease removes all RNA primers from the original strands. These primers are then replaced with appropriate bases. Another exonuclease “proofreads” the newly formed DNA to check, remove and replace any errors. Another enzyme called DNA ligase joins Okazaki fragments together forming a single unified strand. The ends of the linear DNA present a problem as DNA polymerase can only add nucleotides in the 5′ to 3′ direction. The ends of the parent strands consist of repeated DNA sequences called telomeres. Transposition is related to replication, recombination and repair. The process of moving from one place to another involves a type of recombination, insertions of transposable elements can cause mutations, and some transpositions are replicative, generating a new copy while leaving the old copy intact. However, this ability to move is a unique property of transposable elements, and warrants treatment by itself. DNA AND RNA • DNA (Deoxyribonucleate) is a chemical substance that plays a role in the succession of information passed down through generations. • In a coded DNA structure / encoded information for the synthesis of all cell proteins • Segments that have their own characteristics in DNA or chromosomes are called GENs • Information from DNA is transmitted from cell to cell through DNA replication
• RNA (ribonucleic acid), resembling DNA but
not the same, • RNA work is processing information encoded in DNA for protein synthesis through transcription and translation DNA AND RNA STRUCTURE • DNA, has a long structure resembling a string of two threads / twins that are wrapped around each other
• Each helical thread of DNA consists of
nucleotides joined to form polynucleotide • Each nucleotide consists of 3 parts, namely: • Cyanin-containing compounds containing nitrogen: purine and pyrimidine • Purines consist of adenine (A) and guanine (G) • Pyrimidine consists of cytosine (S) and thymine (T) • Five-carbon sugar group (pentose): deoxyribose and • One phosphate molecule (P • The three parts are linked together in order: • alkaline-deoxyribose-phosphate base • In DNA there are 4 types of nucleotides (A, G, S, T) • In DNA there are 2 complementary bases of A- T or G-S, with a ratio always 1: 1 • Other base comparisons are not always 1: 1, such as A-S, S-T, A-T or G-S, the comparison values are very specific / specific for each type of organism, so the value is used to identify and group bacterial taxon • It is these two base pairs A-T and G-S that unite the two helices through hydrogen bonds, between A and T are connected by 2 H bonds, while in G-S by 3 H bonds
• In the VIRUS there is no comparison of 1: 1
between A-T or G-S, so that the DNA in the virus is single-stranded, which is not found in other organisms • RNA, ribonucleic acid, is different from DNA, namely: • RNA, in general, is single
• The sugar component in RNA is ribose not
deoxyribose • Ribose is similar to deoxyribose except for the presence of a hydroxyl group on number 2 carbon atom
• The pyrimidine-based base found in RNA is Urasil (U)
not Timin BIOSYNTHESIS DNA • In general microbes / bacteria can synthesize nucleotides from simple nutrients such as glucose, ammonium sulfate, minerals • In certain bacteria, nucleotides must be supplied in a medium in a finished form • kinase • Nucleotide + ATP nucleotide-P + ADP • kinase • Nucleotide-P + ATP nucleotide-in P + ADP DNA REPLICATION • The bacterial chromosome is a double-molecule DNA molecule, • The molecular weight is 2.5 x 106 Dalton (1 Dalton = mass of 1 H atom) • 1.25 mm (1250 mm) chromosome length • Amount of DNA on chromosomes: 4 x 106 • DNA replication starts from the growing point, where DNA strands separate at that point, forming Y, replicating in sequence from that point in one direction and two directions • The growth point is attached to the cell membrane • In DNA replication, one pacing is needed, namely an RNA primer synthesized by RNA polymerase, in the presence of the primer, the DNA polymerase can synthesize deoxyribonucleotide. • DNA replication, which is not continuous, but in small segments called Okazaki fragments, these segments are joined by DNA ligase enzymes • When the growing point has moved throughout the length of the mol. DNA is formed 2 moles of intact DNA BIOSYNTHESIS PROTEIN • Nucleotide is a constituent of DNA • Ad amino acid protein constituent • DNA is composed by 4 nucleotides • Proteins are composed by> 20 types of axes. Amino • Each microbe is different in its ability to synthesize as. amino for pt synthesis, there are those that are directly from nutrition and there must be amino acids added to the medium • Pt biosynthesis occurs in large ribosomes / RNA particles (rRNA) GENOTIP CHANGE / MUTATION • Genotypic changes / gene mutations can occur in all microbes • Mutations are changes in the nucleotide sequence of a gene that give rise to new genetic characteristics, or other genotypes. The cell / organism is called a mutant • Mutations can occur spontaneously, RECOMBINATION • Genetic recombination is the formation of a new genotype after the exchange of genetic material between 2 different chromosomes • Gene recombination is produced by 3 types of gene transfer, namely: • Conjugation, transfer of genes between cells that physically contact each other • Transduction, transfer of bacteriophage host cell genes into chrome. new host cell • Transformation, transfer of pure DNA from one cell to another, DNA-giving cells will experience lysis
• In bacterial recombination, the cell does not
melt, usually part of the donor cell chromosome is transferred to the recipient cell. Then this recipient cell becomes merozygous, a partially diploid zygote GENETIC OF MICROORGANISM CREATED BY: GROUP 5 INDAH SITANGGANG ENNI D. SIHOTANG NURLAINI NASUTION GHUFRAN ARISYI Figure 9.1. Possible effects of movement of a transposable element in the function and expression of the target gene. The transposable element is shown as a red rectangle, and the target gene (X) is composed of multiple exons. Protein coding regions of exons are green and untranslated regions are gold. The angled arrow indicates the start site for transcription. Transposable elements can cause deletions or inversions of DNA. When transposition generates two copies of the same sequence in the same orientation, recombination can delete the DNA between them. If the two copies are in the opposite orientations, recombination will invert the DNA between them. As part of the mechanism of transposition, additional DNA sequences can be mobilized. DNA located between two copies of a transposable element can be moved together with them when they move. In this manner, transposition can move DNA sequences that are not normally part of a transposable element to new locations. Indeed, "host" sequences can be acquired by viruses and propagated by infection of other individuals Transposition occurs by insertion into a staggered break in a chromosome Since the FDRs are distinctive for each copy, they are not part of the transposable element themselves. Some families of transposable elements do have repeated sequences at their flanks that are identical for all members of the family, but these are integral parts of the transposable element. The variation in sequence of the FDRs indicates that they are generated from the target sites for the transposition events. If the transposable element inserted into a break in the chromosome that left a short overhang (one strand longer than the other), and this overhang were filled in by DNA polymerase as part of the transposition, then the sequence of that overhang would be duplicated on each side of the new copy. Such a break with an overhang is called a staggered break. The size of the staggered break would determine the size of the FDR. REFERENCE