Professional Documents
Culture Documents
ANDHRA PRADESH
Name : J Srinivasa Rao
Designation : Lecturer
Branch : D.A.E.I.E
Institute : Govt. Polytechnic, Kothagudem
Year/Semester : VI Sem
Subject : Analytical Instrumentation
Subject Code : AEI-603
Topic : Introduction to Analytical
Instrumentation and
spectrophotometers
Duration : 100 Min
Sub Topic : Beer Lambert’s Law
Teaching Aids : PPT, Pictures
AEIE-603.3 TO 4 1
Objectives
AEIE-603.3 TO 4 2
Beer-Lambert Law
AEIE-603.3 TO 4 3
Interaction mater with radiation
AEIE-603.3 TO 4 4
Beers Law
A = abc
Where
A is absorbance
a is the absorbtivity (L mol-1 cm-1)
b is the path length of the sample - that is, the path length of
the cuvette in which the sample is contained. (cms)
c is the concentration of the compound in solution, (mol L-1)
AEIE-603.3 TO 4 5
Relationship between absorbance and
transmittance
AEIE-603.3 TO 4 6
• If all the light passes through a solution without
given by A = log10 1 / %T
AEIE-603.3 TO 4 7
%T Vs Path Length
AEIE-603.3 TO 4 8
Absorbance Vs Path length
AEIE-603.3 TO 4 9
A Vs Concentration
AEIE-603.3 TO 4 10
Limitations of Beer’s Law
AEIE-603.3 TO 4 11
Limitations of Beer’s Law
AEIE-603.3 TO 4 12
Limitations (contd)
AEIE-603.3 TO 4 13
Quiz
1. Which of the following relationships between absorbance
and %Transmittance is correct ?
a) A = log10 100 / %T
b) A = 2 - log10 %T
c) A = log10 1 / %T
AEIE-603.3 TO 4 14
Quiz
a) Absorbtivity
b) Molar absorbtivity
c) Path length
Ans. b
AEIE-603.3 TO 4 15
Quiz
3. Why is it generally preferable to use absorbance as a
measure of absorption rather than % Transmittance?
a) Because %T cannot be measured as accurately as
absorbance
b) Because %T is dependant on the power of the
incident radiation
c) Because absorbance is proportional to the
concentration of the analyte, whereas %T is not.
Ans. C
AEIE-603.3 TO 4 16
Quiz
Ans. a
AEIE-603.3 TO 4 17
Summary
• The Beer-Lambert law is the linear relationship between
absorbance and concentration of an absorber of
electromagnetic radiation.
• Beers law is given by the equation A=abc
AEIE-603.3 TO 4 18
Frequently asked questions
AEIE-603.3 TO 4 19